Article
Developmental Biology
Daniel Castranova, Bakary Samasa, Marina Venero Galanternik, Aniket Gore, Allison E. Goldstein, Jong S. Park, Brant M. Weinstein
Summary: The zebrafish has become a widely used animal model due to its accessibility to and usefulness for high-resolution optical imaging. Although historically focused on early development, it is increasingly being used to study processes in juvenile and adult animals. However, imaging of live adult zebrafish is extremely challenging.
Article
Medicine, General & Internal
Michel Paques, Nathaniel Norberg, Celine Chaumette, Florian Sennlaub, Ethan Rossi, Yse Borella, Kate Grieve
Summary: Time-lapse imaging of GA progression revealed extensive changes in the pattern of marginal hyperreflective spots, suggesting a centrifugal motion ahead of atrophy. This process likely reflects the underlying inflammatory and degenerative process. Multimodal time-lapse imaging is valuable for documenting cell-scale dynamics during GA progression.
FRONTIERS IN MEDICINE
(2022)
Review
Biotechnology & Applied Microbiology
Paige Allard, Fotini Papazotos, Laurent Potvin-Trottier
Summary: Cells are dynamic, and the development of microfluidic methods allows for tracking and long-term observation of bacterial cells. This review focuses on the recent developments of a microfluidic device known as the mother machine, and discusses the associated analysis methods and modifications to the experimental setup.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Romain Fernandez, Cedric Moisy
Summary: The increasing interest in biomedical 3D imaging devices from animal and plant research communities has led to new research topics. Combining outputs from imaging devices such as X-ray CT and MRI scans allows non-destructive observation of tissue anatomy, structure, and function in time-lapse multimodal imaging experiments.
Living samples cannot remain in these devices for long periods, leading to variations in shape, position, and orientation in the acquired images. A preprocessing step of 3D registration is required to address these variations before analysis. Fijiyama, a Fiji plugin built upon biomedical registration algorithms, aims to facilitate automatic alignment of 3D images for non-specialists.
Article
Environmental Sciences
Ana Luisa Pires Moreira, Juliana Alves Costa Ribeiro Souza, Jessica Ferreira de Souza, Joao Paulo Medeiros Mamede, Davi Farias, Ana Carolina Luchiari
Summary: Exposure to BP-3 during embryonic development in zebrafish resulted in anxiety-like behavior and reduced social preference, with no change in aggressiveness. Overall, BP-3 exposure led to altered enzymatic activity, persisting into adulthood. GST activity increased in embryos and adults, while CAT activity decreased in both life stages. AChE activity was enhanced only in the larval stage (96 hpf).
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Biochemistry & Molecular Biology
Tongchao Li, Tian-Ming Fu, Kenneth Kin Lam Wong, Hongjie Li, Qijing Xie, David J. Luginbuhl, Mark J. Wagner, Eric Betzig, Liqun Luo
Summary: The study investigates the dynamic cellular processes by which olfactory receptor neurons target axons to specific glomeruli in the olfactory circuit of Drosophila. Through time-lapse imaging and fast imaging techniques, the researchers identified the mechanisms involved in ipsilateral targeting and revealed the dynamics of axon branching upon approaching the target. These observations provide insights into the cellular basis of wiring specificity establishment.
Article
Chemistry, Physical
Bikei Ryu, Yoshikazu Okada, Nobuko Fujita, Yasuko Nagasaka
Summary: We developed an MRI-compatible titanium (Ti) reinforced endotracheal tube (ET) to overcome the artifacts and heat produced by the standard stainless steel (SS) helical wire during MRI. This Ti-alloy-reinforced ET was successfully used in six patients undergoing neurosurgery, allowing them to safely undergo MRI without notable adverse effects.
Article
Biotechnology & Applied Microbiology
Xinyang Li, Yixin Li, Yiliang Zhou, Jiamin Wu, Zhifeng Zhao, Jiaqi Fan, Fei Deng, Zhaofa Wu, Guihua Xiao, Jing He, Yuanlong Zhang, Guoxun Zhang, Xiaowan Hu, Xingye Chen, Yi Zhang, Hui Qiao, Hao Xie, Yulong Li, Haoqian Wang, Lu Fang, Qionghai Dai
Summary: DeepCAD-RT is a real-time denoising method for fluorescence time-lapse images, which uses self-supervised deep learning for noise suppression. Compared to traditional methods, DeepCAD-RT significantly reduces the number of network parameters, memory consumption, and processing time, and achieves high imaging signal-to-noise ratio with one-tenth of the photons. In various photon-limited experiments, DeepCAD-RT demonstrates its utility in morphological and functional interrogation of biological dynamics.
NATURE BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Yingjie Qiao, Jiayi He, Ping Han, Jiangbo Qu, Xubo Wang, Jun Wang
Summary: In this study, adult zebrafish were exposed to triclosan for 150 days, and it was found that long-term exposure to triclosan caused endocrine disruption, reduced germ cell count, and decreased hatching rate and survival rate of offspring. This research provided important insights into the potential diseases caused by triclosan exposure in the aquatic environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Developmental Biology
Hessel Honkoop, Phong D. Nguyen, Veronique E. M. van der Velden, Katharina F. Sonnen, Jeroen Bakkers
Summary: In this study, a cardiac slice culture system was developed to enable real-time imaging of native cardiomyocyte dynamics, allowing for the discovery of cellular mechanisms during heart regeneration. This system provides a new research method for studying cardiomyocyte proliferation and allows for interventions with drugs.
Article
Obstetrics & Gynecology
Carla Gimenez, Laura Conversa, Lucia Murria, Marcos Meseguer
Summary: Time-lapse imaging is a non-invasive method that allows continuous monitoring of embryo development. It has provided new markers for embryo selection and can be used to create predictive models of in vitro fertilization outcomes. The review of 47 articles shows that morphokinetics of the embryo during in vitro development can predict the ability to reach blastocyst stage, implantation and pregnancy rates, live birth outcome, and embryo ploidy.
FERTILITY AND STERILITY
(2023)
Review
Oncology
Maria F. Ramirez, Juan P. Cata
Summary: Surgery is crucial for solid tumor treatment, but it can also elevate the risk of cancer metastasis and recurrence. Clinical evidence suggests that regional anesthesia may not have a significant impact on oncologic outcomes, and ongoing randomized controlled trials aim to clarify the influence of different anesthesia techniques on cancer progression.
FRONTIERS IN ONCOLOGY
(2021)
Article
Engineering, Chemical
Paulo Lopez, Travis Mitchel, Christopher Leonardi, Italo Onederra
Summary: Solution flow has a significant impact on metal recovery and kinetics in leaching processes, especially in heap/dump/insitu leaching. However, most of the research has focused on inter-particle fluid flow, neglecting the capillary rise behavior within rock samples. This study presents a simple methodology using Time Lapse Digital Imaging to estimate capillary action in different rock types. The method is sensitive to pixel variation, which indicates changes in wettability. It can be combined with other technologies to better understand how pore size distribution influences capillary response and fluid movement in ore.
MINERALS ENGINEERING
(2023)
Article
Environmental Sciences
Utsav N. Patel, Urvesh D. Patel, Aniket Khadayata, Rahul K. Vaja, Chirag M. Modi, Harshad B. Patel
Summary: The toxicity of a binary mixture of cadmium and mercury on the ovary of adult zebrafish was evaluated. The mixture exposure caused lipid peroxidation and damage to different stages of oocytes in the ovary.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Laura Hermans, Murat Kaynak, Jonas Braun, Victor Lobato Rios, Chin-Lin Chen, Adam Friedberg, Semih Guenel, Florian Aymanns, Mahmut Selman Sakar, Pavan Ramdya
Summary: Understanding biological networks requires minimally invasive methods to repeatedly record neural circuits in behaving animals. The authors have developed devices for long-term optical recordings in adult Drosophila ventral nerve cord, showing minimal impact on animal behavior and survival, and demonstrating the importance of tracking neural activity.
NATURE COMMUNICATIONS
(2022)
Review
Cardiac & Cardiovascular Systems
Andrea Caporali, Magnus Back, Mat J. Daemen, Imo E. Hoefer, Elizabeth A. Jones, Esther Lutgens, Christian M. Matter, Marie-Luce Bochaton-Piallat, Arndt F. Siekmann, Judith C. Sluimer, Sabine Steffens, Jose Tunon, Cecile Vindis, Jolanda J. Wentzel, Seppo Yla-Herttuala, Paul C. Evans
CARDIOVASCULAR RESEARCH
(2018)
Review
Hematology
Wade W. Sugden, Arndt F. Siekmann
CURRENT OPINION IN HEMATOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Kristy Red-Horse, Arndt F. Siekmann
Article
Cardiac & Cardiovascular Systems
Olga Bondareva, Roman Tsaryk, Vesna Bojovic, Maria Odenthal-Schnittler, Arndt F. Siekmann, Hans-J. Schnittler
CARDIOVASCULAR RESEARCH
(2019)
Article
Cell Biology
Massimo M. Santoro, Monica Beltrame, Daniela Panakova, Arndt F. Siekmann, Natascia Tiso, Marina Venero Galanternik, Hyun Min Jung, Brant M. Weinstein
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2019)
Article
Hematology
Cao Nguyen Duong, Astrid F. Nottebaum, Stefan Butz, Stefan Volkery, Dagmar Zeuschner, Martin Stehling, Dietmar Vestweber
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2020)
Article
Hematology
Laura J. Braun, Rebekka I. Stegmeyer, Kerstin Schaefer, Stefan Volkery, Silke M. Currie, Birgit Kempe, Astrid F. Nottebaum, Dietmar Vestweber
Article
Cell Biology
Peng Gao, Changya Chen, Elizabeth D. Howell, Yan Li, Joanna Tober, Yasin Uzun, Bing He, Long Gao, Qin Zhu, Arndt F. Siekmann, Nancy A. Speck, Kai Tan
GENES & DEVELOPMENT
(2020)
Article
Biochemistry & Molecular Biology
William Benman, Erin E. Berlew, Hao Deng, Caitlyn Parker, Ivan A. Kuznetsov, Bomyi Lim, Arndt F. Siekmann, Brian Y. Chow, Lukasz J. Bugaj
Summary: The study introduces single-component optogenetic probes with activation dynamics dependent on both light and temperature. The BcLOV4 photoreceptor was utilized to stimulate signaling in mammalian cells, showing tunable membrane translocation dynamics in response to light and temperature. Quantitative modeling predicted the activation dynamics of BcLOV4 and demonstrated its versatility in generating widely applicable optogenetic tools.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Developmental Biology
Elvin Leonard, Ricardo J. Figueroa, Jeroen Bussmann, Nathan D. Lawson, Julio D. Amigo, Arndt F. Siekmann
Summary: The study reveals the important role of Pdgfrb signaling in mural cell recruitment and arterial development. Mural cells have different expression characteristics and origins in different locations. Moreover, newly formed mural cells during tissue regeneration do not derive from pre-existing cells.
Article
Developmental Biology
Martin Lange, Nils Ohnesorge, Dennis Hoffmann, Susana F. Rocha, Rui Benedito, Arndt F. Siekmann
Summary: Studies in zebrafish show that vegfaa and vegfab have different effects on endothelial cell migration and proliferation. Mutations in vegfaa impact both migration and proliferation, while mutations in vegfab more strongly affect the proliferation of endothelial cells in specific blood vessels, potentially through the PI3K signaling pathway.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Dermatology
Silke M. Currie, Rebekka Stegmeyer, Karina Mildner, Leonhard Breitsprecher, Dagmar Zeuschner, Olympia Ekaterini Psathaki, Kerstin Schafer, Markus Wilkens, Stefan Volkery, Bernhard Nieswandt, Dietmar Vestweber
Summary: Platelets prevent neutrophil-induced hemorrhage in immune complex-mediated skin inflammation, preserving vascular integrity. By studying the single-cell dynamics of this process in real-time, researchers gained further insights into platelet recruitment and bleeding events.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Roman Tsaryk, Nora Yucel, Elvin Leonard, Noelia Diaz, Olga Bondareva, Maria Odenthal-Schnittler, Zoltan Arany, Juan M. Vaquerizas, Hans Schnittler, Arndt F. Siekmann
Summary: This study investigates the transcriptional regulation of endothelial cells (ECs) in response to shear stress. The researchers performed ChIP-Seq, ATAC-Seq, and RNA-Seq experiments on human umbilical vein endothelial cells (HUVEC) exposed to shear stress and characterized the shear stress response in zebrafish embryo ECs. The findings provide insights into shear stress-responsive elements in controlling EC biology.
SCIENTIFIC REPORTS
(2022)
Article
Developmental Biology
Elvin Leonard, Sana Safatul Hasan, Arndt F. Siekmann
Summary: Blood vessels in the zebrafish caudal fin form through a stepwise process involving different morphogenetic processes, including artery generation from vein-derived endothelial cells, production of veins by veins, formation of distinct vascular plexuses, and angiogenesis. These processes are regulated by a combination of signaling pathways and local cues. The study provides insights into the diverse mechanisms underlying the assembly of mature vasculature.
Proceedings Paper
Cardiac & Cardiovascular Systems
Robert Meissner, Wade W. Sugden, Arndt F. Siekmann, Cornelia Denz
DIAGNOSTIC AND THERAPEUTIC APPLICATIONS OF LIGHT IN CARDIOLOGY 2018
(2018)