Article
Plant Sciences
Suzuka Kikuchi, Takuya Sakamoto, Sachihiro Matsunaga, Akitoshi Iwamoto
Summary: Whole-mount fluorescent in situ hybridization (WM-FISH) is a useful technique for studying chromosome behavior in tissues or organs. However, it is challenging to obtain precise spatial information in roots due to the damage caused during processing. In this study, a new approach was developed to perform WM-FISH on intact roots of Arabidopsis thaliana, allowing for accurate analysis of nuclear size and centromere signals. The method involved direct hybridization on MAS-coated glass slides and optimized enzyme treatment conditions. 3D data processing was utilized to quantify parameters and determine spatial profiles.
JOURNAL OF PLANT RESEARCH
(2023)
Review
Plant Sciences
Jason Sims, Peter Schlogelhofer, Marie-Therese Kurzbauer
Summary: Visualization of meiotic chromosomes and proteins has become crucial for studying meiosis, and recent advances in super-resolution technologies have enabled observation of cells and nuclei at nanometer scale, allowing researchers to observe complex meiotic molecular processes in unprecedented detail.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Ling Cao, Sheng Wang, Lihua Zhao, Yuan Qin, Hong Wang, Yan Cheng
Summary: UBC22 is critical for normal chromosome segregation in female meiosis but not for male meiosis, and DMC1 distribution is abnormal in mutant female meiocytes, leading to frequent aneuploids among the mutant plants due to abnormal DNA content in female mutant gametes. These results provide important leads for studying the role of UBC22 and K11-linked ubiquitination.
Article
Multidisciplinary Sciences
Longfei Zhu, Nadia Fernandez-Jimenez, Maja Szymanska-Lejman, Alexandre Pele, Charles J. Underwood, Heidi Serra, Christophe Lambing, Julia Dluzewska, Tomasz Bieluszewski, Monica Pradillo, Ian R. Henderson, Piotr A. Ziolkowski
Summary: This study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants, showing the impact of SNI1 mutations and interactions with other genes on crossover distribution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Matteo Vitale, Jiangtao Liang, Igor Sharakhov, Federica Bernardini
Summary: Spermatogenesis is a complex biological process that is important for understanding and developing genetic technologies. Whole-mount fluorescence in situ hybridization (WFISH) is a method that allows researchers to study the changes in cell nuclear structure during spermatogenesis, while preserving the native cytological structure and enabling high signal detection.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemistry & Molecular Biology
Liang-Jian Chen, Na-Na Zhang, Chun-Xiang Zhou, Zhi-Xia Yang, Yan-Ru Li, Teng Zhang, Cong-Rong Li, Xin Wang, Yang Wang, Zi-Bin Wang, Zheng-Rong Xia, Zhen-Bo Wang, Cui-Lian Zhang, Yi-Chun Guan, Qing-Yuan Sun, Dong Zhang
Summary: This study reveals the critical role of Gm364 in female fertility, where its knockout results in decreased follicle numbers, impaired oocyte quality, reduced oocyte maturation, and increased aneuploidy. Mechanistically, Gm364 interacts with MIB2 to activate the Notch2-AKT signaling pathway, influencing oocyte meiosis and quality.
CELL DEATH AND DIFFERENTIATION
(2022)
Review
Cell Biology
Qiaojuan Mei, Huiying Li, Yu Liu, Xiaofei Wang, Wenpei Xiang
Summary: CDC42, a member of the Rho-GTPase family, plays a crucial role in establishing polarity and ensuring proper gametogenesis in female vertebrate oocytes. Through its local occupation of specific regions and synergistic regulation in a multilevel signalling network, CDC42 ensures precise assembly of the contractile ring at the right time and place for accurate gametogenesis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Mohammad Aslam, Beenish Fakher, Yuan Qin
Summary: In living organisms, sexual reproduction relies on the successful development of gametes, with flowering plants producing gametes in specialized organs of the flower, the gametophytes. Recent evidence suggests that small RNA-mediated pathways play important roles in the development and specification of the female gametophyte. Numerous protein complexes, molecular, and genetic mechanisms are involved in tightly regulating the development of the female gametophyte, known as the embryo sac, in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Genetics & Heredity
Jessica E. Fellmeth, Kim S. McKim
Summary: Drosophila has been a valuable model organism for studying meiosis, particularly in females, due to its genetic and cytological tools. It is also a good model for mammalian meiosis due to biological similarities.
Article
Cell Biology
Tereza Pauerova, Lenka Radonova, Adela Horakova, Jason G. G. Knott, Martin Anger
Summary: The study found that the expression of securin protein in oocytes during female meiosis is compartmentalized, accumulating on the spindle during meiosis I, possibly impacting the regulation of separase activity directly.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marina Martinez-Garcia, Charles I. White, F. Chris. H. Franklin, Eugenio Sanchez-Moran
Summary: TOPII plays an essential endogenous role in normal root growth, cell cycle regulation, mitotic DNA repair through homologous recombination, and meiotic DNA repair progression in Arabidopsis thaliana. While it is required for meiotic double-strand break repair, it does not affect crossover formation. It is proposed that TOPII may facilitate mitotic homologous recombination DNA repair by relieving stress necessary for strand invasion and D-loop formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Tereza Pauerova, Lenka Radonova, Adela Horakova, Jason G. Knott, Martin Anger
Summary: Chromosome segregation during female meiosis is crucial for proper cell division, with the study focusing on the role of the protein inhibitor securin in regulating separase activity. The research revealed compartmentalized expression of securin in oocytes, suggesting a potential impact on separase regulation near the chromosomes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Luping Yu, Huiru Zhang, Xuebing Guan, Dongdong Qin, Jian Zhou, Xin Wu
Summary: The study shows that the loss of Esrp1 leads to female infertility in mice, primarily affecting spindle organization, chromosome alignment, and oocyte meiosis. Single-cell RNA-seq analysis revealed 528 aberrant alternative splicing events associated with microtubule cytoskeletal organization.
Article
Biology
Lingli Huang, Wenqing Li, Xingxing Dai, Shuai Zhao, Bo Xu, Fengsong Wang, Ren-Tao Jin, Lihua Luo, Limin Wu, Xue Jiang, Yu Cheng, Jiaqi Zou, Caoling Xu, Xianhong Tong, Heng-Yu Fan, Han Zhao, Jianqiang Bao
Summary: This study identified and characterized novel biallelic variants in MAD2L1BP responsible for oocyte maturation arrest at MI, providing new therapeutic avenues for female primary infertility.
Article
Developmental Biology
Alicia McCarthy, Kahini Sarkar, Elliot T. Martin, Maitreyi Upadhyay, Seoyeon Jang, Nathan D. Williams, Paolo E. Forni, Michael Buszczak, Prashanth Rangan
Summary: The study reveals that Msl3, in cooperation with ATAC and Set2, regulates germ cell differentiation in the female reproductive system of Drosophila. Msl3 controls GSC differentiation by modulating translation of the key factor Rbfox1.
Article
Evolutionary Biology
Bernardo Perez-Zamorano, Miguel Vallebueno-Estrada, Javier Martinez Gonzalez, Angel Garcia Cook, Rafael Montiel, Jean-Philippe Vielle-Calzada, Luis Delaye
GENOME BIOLOGY AND EVOLUTION
(2017)
Article
Plant Sciences
Aurelie Chambon, Allan West, Daniel Vezon, Christine Horlow, Arnaud De Muyt, Liudmila Chelysheva, Arnaud Ronceret, Alice Darbyshire, Kim Osman, Stefan Heckmann, F. Chris H. Franklin, Mathilde Grelon
Article
Genetics & Heredity
Daniel Rodriguez-Leal, Cao Xu, Choon-Tak Kwon, Cara Soyars, Edgar Demesa-Arevalo, Jarrett Man, Lei Liu, Zachary H. Lemmon, Daniel S. Jones, Joyce Van Eck, David P. Jackson, Madelaine E. Bartlett, Zachary L. Nimchuk, Zachary B. Lippman
Article
Biochemistry & Molecular Biology
Marek Sirl, Tereza Snajdrova, Dolores Gutierrez-Alanis, Joseph G. Dubrovsky, Jean Phillipe Vielle-Calzada, Ivan Kulich, Ales Soukup
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Genetics & Heredity
Jia-Chi Ku, Arnaud Ronceret, Inna Golubovskaya, Ding Hua Lee, Chiting Wang, Ljudmilla Timofejeva, Yu-Hsin Kao, Ana Karen Gomez Angoa, Karl Kremling, Rosalind Williams-Carrier, Robert Meeley, Alice Barkan, W. Zacheus Cande, Chung-Ju Rachel Wang
Article
Biochemical Research Methods
Martina Juranic, Dilrukshi S. K. Nagahatenna, Rigel Salinas-Gamboa, Melanie L. Hand, Nidia Sanchez-Leon, Weng Herng Leong, Tracy How, Natalia Bazanova, Andrew Spriggs, Jean-Philippe Vielle-Calzada, Anna M. G. Koltunow
Article
Biology
Takayoshi Ishii, Martina Juranic, Shamoni Maheshwari, Fernanda de Oliveira Bustamante, Maximilian Vogt, Rigel Salinas-Gamboa, Steven Dreissig, Nial Gursanscky, Tracy How, Dmitri Demidov, Joerg Fuchs, Veit Schubert, Andrew Spriggs, Jean-Philippe Vielle-Calzada, Luca Comai, Anna M. G. Koltunow, Andreas Houben
COMMUNICATIONS BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Anat Hendelman, Sophia Zebell, Daniel Rodriguez-Leal, Noah Dukler, Gina Robitaille, Xuelin Wu, Jamie Kostyun, Lior Tal, Peipei Wang, Madelaine E. Bartlett, Yuval Eshed, Idan Efroni, Zachary B. Lippman
Summary: The study explores the functional divergence of WOX9 in tomato and reveals hidden pleiotropic roles through a comprehensive allelic series. Mimicking these alleles in Arabidopsis exposes new inflorescence phenotypes, indicating deeply conserved pleiotropy in gene function. The findings suggest that targeted cis-regulatory mutations can uncover conserved gene functions and reduce undesirable effects in crop improvement.
Article
Plant Sciences
Ping Che, Shujun Chang, Marissa K. Simon, Zhifen Zhang, Ahmed Shaharyar, Jesse Ourada, Dennis O'Neill, Mijael Torres-Mendoza, Yinping Guo, Kathleen M. Marasigan, Jean-Philippe Vielle-Calzada, Peggy Ozias-Akins, Marc C. Albertsen, Todd J. Jones
Summary: This study presents a highly efficient transformation system for cowpea using embryonic axis explants, which achieved efficient gene delivery, transgenic selection, and transgenic plant generation. By removing the shoot apical meristem from the explants, direct multiple shoot organogenesis from the cotyledonary node tissue was stimulated.
Article
Biochemistry & Molecular Biology
Nathalie Vrielynck, Katja Schneider, Marion Rodriguez, Jason Sims, Aurelie Chambon, Aurelie Hurel, Arnaud De Muyt, Arnaud Ronceret, Ondrej Krsicka, Christine Mezard, Peter Schlogelhofer, Mathilde Grelon
Summary: This study reveals the functions and interactions of different proteins in the process of meiotic recombination initiation, elucidating their critical roles in DSB formation and resection processes.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Sergio Alan Cervantes-Perez, Lenin Yong-Villalobos, Nathalia M. V. Florez-Zapata, Araceli Oropeza-Aburto, Felix Rico-Resendiz, Itzel Amasende-Morales, Tianying Lan, Octavio Martinez, Jean Philippe Vielle-Calzada, Victor A. Albert, Luis Herrera-Estrella
Summary: This study reveals that compared to other angiosperms, Utricularia gibba has an unusual distribution of small RNAs and reduced global DNA methylation levels. Moreover, key genes involved in the RdDM pathway may lack compensatory paralogs or contain truncated proteins, potentially impacting the developmental processes in U. gibba.
SCIENTIFIC REPORTS
(2021)
Review
Plant Sciences
Yared Gutierrez Pinzon, Jose Kenyi Gonzalez Kise, Patricia Rueda, Arnaud Ronceret
Summary: The segregation of homologous chromosomes depends on their recombination, and the absence of meiotic checkpoints in plants allows observation of subsequent meiotic events, aiding in the understanding of recombination and bivalent formation mechanisms in plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Cell Biology
Nahid Rafiei, Arnaud Ronceret
Summary: Plants have played a crucial role in our understanding of fundamental biological principles. Recent plant studies have shed new light on the mysterious mechanisms of meiotic crossover interference, heterochiasmy, obligatory crossover, and crossover homeostasis. These findings have contributed to our understanding of key biological concepts.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Plant Sciences
Xingang Wang, Lyndsey Aguirre, Daniel Rodriguez-Leal, Anat Hendelman, Matthias Benoit, Zachary B. Lippman
Summary: The study found that targeted mutations in conserved promoter sequences of the repressor of stem cell proliferation, SlCLV3, have a weak impact on fruit locule number, but pairwise combinations of these mutations mildly enhance this trait, revealing additive and synergistic relationships. Conversely, the positive regulator of stem cell proliferation, SlWUS, is more tolerant to promoter perturbations. The results suggest that complex interactions among cis-regulatory variants can shape quantitative variation in crop traits.
Article
Plant Sciences
Zachary H. Lemmon, Nathan T. Reem, Justin Dalrymple, Sebastian Soyk, Kerry E. Swartwood, Daniel Rodriguez-Leal, Joyce Van Eck, Zachary B. Lippman