期刊
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY
卷 18, 期 1-2, 页码 146-155出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10498850802584280
关键词
shrimp; species authentication; peptide mass fingerprint; MALDI-TOF MS
资金
- Instituto Nacional de Investigaciones Agrarias [CAL03-030-C2-2]
- PGIDIT Research Program [PGIDIT04RMA261004PR]
- Spanish Ministry of Education and Science [AP-2004-5826]
Tiger prawn, Penaeus monodon, and banana white Prawn, Fenneropenaeus indicus-two of the most important commercial shrimp species-share world distribution, habitat, and similar morphometric characters. Because of commercial regulations about labeling and traceability requirements, species authentication methods are of great interest to fisheries and seafood industries. In this study, peptide mass fingerprints of both species are compared, using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) after trypsin digestion of a protein resolved by two-dimensional gel electrophoresis. Though low variability among these species is observed, two peptides of mass 1077.6 and 1205.7 Da are found to be useful to differentiate them.
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