Article
Biology
Lise M. Sjogaard-Frich, Andreas Prestel, Emilie S. Pedersen, Marc Severin, Kristian Kolby Kristensen, Johan G. Olsen, Birthe B. Kragelund, Stine Falsig Pedersen
Summary: Through various biophysical techniques, this study reveals the complex interaction mechanism between CaM and NHE1, identifying multiple states and structures of the NHE1:CaM complex that can be modulated by stoichiometries, calcium concentrations, and phosphorylation. Experimental results suggest that CaM may enhance NHE1 regulation and indicate that this structural diversity is relevant to other CaM complexes as well.
Article
Medicine, Research & Experimental
Laween Uthman, Xiaoling Li, Antonius Baartscheer, Cees A. Schumacher, Patricia Baumgart, Jeroen Hermanides, Benedikt Preckel, Markus W. Hollmann, Ruben Coronel, Coert J. Zuurbier, Nina C. Weber
Summary: Inflammation leading to oxidative stress in endothelial cells is linked to heart failure development. The study suggests that TNF-alpha can activate NHE to raise [Na+](c), triggering ROS production, and EMPA can reduce inflammation-induced ROS by inhibiting NHE activity.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Cardiac & Cardiovascular Systems
Yu Jin Chung, Kyung Chan Park, Sergiy Tokar, Thomas R. Eykyn, William Fuller, Davor Pavlovic, Pawel Swietach, Michael J. Shattock
Summary: Research indicates that cardiac NHE1 activity is not inhibited by EMPA (or other SGLT2i's) and EMPA has no effect on [Na+] over a wide range of concentrations, including the therapeutic dose.
CARDIOVASCULAR RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Xiuju Li, Tommy Tu, Sicheng Quan, Francisco J. Quintero, Richard Fahlman, Larry Fliegel
Summary: This study investigated the effects of mutating two amino acids in the regulatory tail of NHE1 protein. The mutations led to changes in protein activity, molecular weight, and conformation, indicating the importance of these amino acids in enzyme kinetics and protein structure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Kiem Vu, Eduardo Blumwald, Angie Gelli
Summary: The study demonstrates that the amiloride analog HMA shows potential as a candidate for combination therapy with various antifungal azole drugs, displaying synergy with posaconazole, voriconazole, and ketoconazole. Additionally, HMA exhibits limited potential as a monotherapy against Cryptococcus, indicating the need for further research on its potential additional targets in fungal cells.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ghassan Bkaily, Yanick Simon, Ashley Jazzar, Houssein Najibeddine, Alexandre Normand, Danielle Jacques
Summary: Our understanding of essential hypertension is extensive, and while its treatment is well-known, not all individuals with hypertension are salt-sensitive. Even normotensive individuals may face increased cardiovascular risks with a high salt diet. Research on the mechanisms and treatment of hypertension sensitive to high-sodium diets is crucial.
Article
Physiology
Alexander M. Clifford, Martin Tresguerres, Greg G. Goss, Chris M. Wood
Summary: During exposure to acidic water, zebrafish utilize a novel mechanism of Na+ uptake that relies on the outwardly directed K+ gradient as a counter-substrate for Na+ and is sensitive to tetraethylammonium (TEA).
Review
Chemistry, Medicinal
Huiting Xia, Aqeela Zahra, Meng Jia, Qun Wang, Yunfu Wang, Susan L. Campbell, Jianping Wu
Summary: In this review, the potential role of NHE1 in cardiac hypertrophy and heart failure is discussed, along with an investigation into the underlying molecular mechanisms.
Article
Nutrition & Dietetics
Ashley Jazzar, Danielle Jacques, Ghassan Bkaily
Summary: Insulin-induced cardiac hypertrophy is associated with changes in Na+ and Ca2+ homeostasis, with taurine pre-treatment preventing these effects. Additionally, chronic taurine treatment prevents insulin-induced morphological and ionic remodeling of cardiomyocytes.
Article
Biochemistry & Molecular Biology
Xiuju Li, Benjamin Buckley, Konstantin Stoletov, Yang Jing, Marie Ranson, John D. Lewis, Mike Kelso, Larry Fliegel
Summary: This study examined the role of NHE1 and uPA in prostate cancer cell migration and colony formation, finding that only uPA activity played a critical role in cell migration and colony formation, while the effect of NHE1 was minor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xu Liu, Yue Qiu, Ning Huang, Yan-Hua Liu, Huan-Huan Wang, Ya-Nan Yu, Yu-Ting Song, Guang-Rui Wan, Shuang-Xi Wang, Peng Li, Ya-Ling Yin
Summary: This study found that CT could alleviate DOX-induced cardiotoxicity in rats by regulating oxidative stress and apoptosis-related factors, as well as inhibiting NHE1 upregulation. The protective effects of CT were affected by the concurrent administration of LiCl.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Review
Physiology
Sarah M. Nwia, Xiao Chun Li, Ana Paula de Oliveira Leite, Rumana Hassan, Jia Long Zhuo
Summary: The sodium/hydrogen exchanger 3 (NHE3) is a vital antiporter involved in Na+/H+ exchange in the small intestines and proximal tubules. It plays key roles in intracellular pH regulation and acid-base balance. Recent studies have shown that NHE3 is responsible for absorbing external Na+ and excreting intracellular H+, contributing to salt and fluid balance, blood pressure homeostasis, and the development of hypertension.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Microbiology
Joao Paulo Ferreira Rodrigues, Leonardo Loch, Thiago Souza Onofre, Nobuko Yoshida
Summary: The involvement of NHE1 in the internalization of Trypanosoma cruzi metacyclic trypomastigotes was studied. NHE1 plays a role in maintaining the activation status of diverse protein kinases and preventing inappropriate F-actin arrangement, thus affecting lysosome distribution.
Article
Pharmacology & Pharmacy
Yixiang Xu, Chao Zhang, Kai Jiang, Xinchun Yang, Feng Chen, Zhiyang Cheng, Jinlong Zhao, Jiaxing Cheng, Xiaokang Li, Xin Chen, Luoyifan Zhou, Hao Duan, Yunyuan Huang, Yaozu Xiang, Jian Li
Summary: To address the issue of separating ant-heart failure (HF) activity from glucose-lowering side-effects, the researchers conducted structural repurposing of the SGLT2 inhibitor EMPA. The optimized derivative JX01 showed weaker SGLT2-inhibitory activity and lower glucose-lowering side-effects, but better NHE1-inhibitory activity and HF mice cardioprotective effect. Furthermore, JX01 demonstrated good safety profiles and pharmacokinetic properties. This study provides a paradigm for discovering novel anti-HF drugs and indirectly highlights the importance of SGLT2-independent molecular mechanisms in the cardioprotective effects of SGLT2 inhibitors.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Biology
Jungmi Choi, Naoki Matoba, Daiki Setoyama, Daiki Watanabe, Yuichiro Ohnishi, Ryuto Yasui, Yuichirou Kitai, Aki Oomachi, Yutaro Kotobuki, Yoichi Nishiya, Michael Paul Pieper, Hiromi Imamura, Motoko Yanagita, Masamichi Yamamoto
Summary: The SGLT2 inhibitor, Empagliflozin, increases mitochondrial ATP levels in a mouse model of diabetes, leading to cardioprotective effects. Empagliflozin, an inhibitor of sodium-glucose co-transporter 2, has been shown to reduce cardiovascular events in patients with type 2 diabetes and established cardiovascular disease. The mechanism behind these effects is still unclear.
COMMUNICATIONS BIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Juan I. Burgos, Alejandra M. Yeves, Jorge P. Barrena, Enrique L. Portiansky, Martin G. Vila-Petroff, Irene L. Ennis
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2017)
Article
Physiology
A. M. Yeves, J. I. Burgos, A. J. Medina, M. C. Villa-Abrille, I. L. Ennis
Article
Cardiac & Cardiovascular Systems
Eduardo Manuel Escudero, Alejandro Orlowski, Alejandro Diaz, Oscar Andres Pinilla, Irene Lucia Ennis, Ernesto Alejandro Aiello
CARDIOLOGY JOURNAL
(2014)
Article
Cell Biology
Carolina D. Garciarena, Juliana C. Fantinelli, Claudia I. Caldiz, Gladys Chiappe de Cingolani, Irene L. Ennis, Nestor G. Perez, Horacio E. Cingolani, Susana M. Mosca
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2011)
Article
Peripheral Vascular Disease
Carolina D. Garciarena, Oscar A. Pinilla, Mariela B. Nolly, Ruben P. Laguens, Eduardo M. Escudero, Horacio E. Cingolani, Irene L. Ennis
Article
Physiology
Carolina D. Garciarena, Claudia I. Caldiz, Maria V. Correa, Guillermo R. Schinella, Susana M. Mosca, Gladys E. Chiappe de Cingolani, Horacio E. Cingolani, Irene L. Ennis
JOURNAL OF APPLIED PHYSIOLOGY
(2008)
Article
Cardiac & Cardiovascular Systems
Alejandro M. Yeves, Maria C. Villa-Abrille, Nestor G. Perez, Andres J. Medina, Eduardo M. Escudero, Irene L. Ennis
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2014)
Article
Physiology
Maria V. Correa, Mariela B. Nolly, Claudia I. Caldiz, Gladys E. Chiappe de Cingolani, Horacio E. Cingolani, Irene L. Ennis
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2014)
Article
Pathology
Andres J. Medina, Oscar A. Pinilla, Enrique L. Portiansky, Claudia I. Caldiz, Irene L. Ennis
EXPERIMENTAL AND MOLECULAR PATHOLOGY
(2019)
Article
Cardiac & Cardiovascular Systems
Irene L. Ennis, Ernesto A. Aiello, Horacio E. Cingolani, Nestor G. Perez
CURRENT CARDIOLOGY REVIEWS
(2013)
Letter
Cardiac & Cardiovascular Systems
Eduardo M. Escudero, Oscar A. Pinilla, Irene L. Ennis
REVISTA ESPANOLA DE CARDIOLOGIA
(2012)
Article
Peripheral Vascular Disease
Nestor G. Perez, Martin R. Piaggio, Irene L. Ennis, Carolina D. Garciarena, Celina Morales, Eduardo M. Escudero, Oscar H. Cingolani, Gladys Chiappe de Cingolani, Xiao-Ping Yang, Horacio E. Cingolani
Review
Cardiac & Cardiovascular Systems
Horacio E. Cingolani, Irene L. Ennis
Article
Cardiac & Cardiovascular Systems
Irene L. Ennis, Horacio E. Cingolani, Carolina D. Garciarena, Maria C. Camilion de Hurtado, Maria C. Villa-Abrille, Ernesto A. Aiello, Nestor G. Perez
CURRENT CARDIOLOGY REVIEWS
(2007)