Article
Food Science & Technology
D. R. Michael, J. Kerry-Smith, T. S. Webberley, K. R. Murphy, S. F. Plummer, L. Parry, J. R. Marchesi
Summary: Conventional tissue culture models often lack physiological relevance. In this exploratory study, dynamic flow conditions were applied to Caco-2 intestinal epithelial cells and their response compared with statically cultured counterparts. The results showed that flow conditions improved the viability, mucin production, and pH tolerance of Caco-2 cells, and altered their response to Gram positive bacteria compared to static conditions.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Microbiology
Pudi Wang, Songbiao Chen, Chengshui Liao, Yanyan Jia, Jing Li, Ke Shang, Jian Chen, Pinghua Cao, Wang Li, Yuanxiao Li, Zuhua Yu, Ke Ding
Summary: This study investigated the adhesion abilities and survival capabilities of six chicken-derived probiotic strains to Caco-2 cells and under adverse conditions. Pediococcus pentosaceus 2-5 and Lactobacillus reuteri L-3 showed strong adhesion abilities and inhibition of enteropathogenic bacteria, making them potential candidates for probiotics.
Article
Food Science & Technology
Perihan Kubra Akman, Gorkem Ozulku, Fatih Tornuk, Hasan Yetim
Summary: This study isolated lactic acid bacteria strains with probiotic properties from traditional Turkish fermented beverages, showing high resistance to low pH and bile salts, as well as variable antimicrobial effects on pathogens. The bacterial cells also exhibited high adhesive ability on intestinal epithelial cells. These findings suggest that the LAB strains have high probiotic potential and could be used in developing functional food products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Radhika Khera, Ahmad R. Mehdipour, Jani R. Bolla, Joerg Kahnt, Sonja Welsch, Ulrich Ermler, Cornelia Muenke, Carol Robinson, Gerhard Hummer, Hao Xie, Hartmut Michel
Summary: Bacteria use AI-2 as a communication molecule to coordinate behaviors, but little is known about its export mechanism. This study reveals the structures and functions of AI-2 exporters through cryogenic electron microscopy and other experiments, providing new insights into the biological activity of AI-2.
Article
Chemistry, Multidisciplinary
Maria Teresa Rocchetti, Pasquale Russo, Giuseppe Spano, Letizia De Santis, Ilenia Iarusso, Nicola De Simone, Samira Brahimi, Daniela Fiocco, Vittorio Capozzi
Summary: This study reversed the traditional approach for probiotic screening by evaluating the in vitro probiotic properties of microbes already commercialized in the dairy sector. The analyzed strains showed good survival under stressful conditions, strong inhibitory activity against harmful bacteria, high adhesion rates, and anti-inflammatory effects.
APPLIED SCIENCES-BASEL
(2022)
Review
Microbiology
Vladimir I. Trukhachev, Victor K. Chmykhalo, Anna A. Belanova, Darya K. Beseda, Michael L. Chikindas, Anzhelika B. Bren, Alexey M. Ermakov, Irina M. Donnik, Marya M. Belousova, Peter V. Zolotukhin
Summary: The development of probiotics for animal farming requires thorough testing of various properties, and using human-derived in vitro models can improve screening efficiency and reduce costs.
VETERINARY MICROBIOLOGY
(2021)
Article
Food Science & Technology
Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu
Summary: In this study, it was found that Ganoderma atrum polysaccharide (PSG-1) indirectly affects dendritic cells' immune function in the intestinal-like Caco-2/DCs co-culture model. The interaction between Caco-2 and DCs was regulated by PSG-1 through TNF-alpha and NF-kappa B signaling pathways of Caco-2 in the model. This suggests a potential mechanism for immune modulation in the gut environment through interactions between intestinal epithelial cells and immune cells.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Engineering, Environmental
Boyan Xu, Qi An Celine Cho, Tze Chiang Albert Ng, Shujuan Huang, How Yong Ng
Summary: This study enriched a facultative QQ consortium for AI-2-based QS disruption and discovered its quorum quenching performance in an AnMBR for membrane fouling retardation. The QQ consortium was able to degrade DPD and prolong membrane filtration operation in treating domestic wastewater. QQ reduced the content of proteins and carbohydrates and altered the composition of EPS and SMP. Moreover, QQ decreased the quantity of organics and planktonic microorganisms in the supernatant and enhanced methane production while reducing sulfate reduction.
Article
Food Science & Technology
Sulaiman F. Aljasir, Dennis J. D'Amico
Summary: Commercially produced protective bacterial cultures (PCs) have the potential to survive human gastrointestinal conditions and exhibit anti-infective properties against L. monocytogenes. They can reduce adhesion, invasion, and cytotoxicity of L. monocytogenes, as well as translocation in Caco-2 cells. PCs also decrease DextranFITC permeability through Caco-2 cells and reduce L. monocytogenes-induced mortality in Caenorhabditis elegans, suggesting probiotic effects beyond inhibiting pathogen growth and virulence in foods.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Hassan Barzegar, Behrooz Alizadeh Behbahani, Fereshteh Falah
Summary: This study investigated probiotic, antimicrobial, technological, and safety properties of lactobacillus strains isolated from local Iranian cheese made from raw milk. Results revealed that L. acidophilus (B14) exhibited the best resistance to low pH and proteolytic activity, suggesting its suitability for use as a coculture in the dairy industry.
FOOD SCIENCE & NUTRITION
(2021)
Article
Food Science & Technology
S. Rodriguez-Sanchez, P. Fernandez-Pacheco, S. Sesena, C. Pintado, M. Ll Palop
Summary: A total of 98 Lactobacillus strains were screened for their antimicrobial and probiotic properties, with 12 strains showing potential in both aspects. These strains, particularly Lb. paracasei Lb38 and Lb. brevis Lb99, could be considered as biocontrol agents in the food industry.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Julie S. Valastyan, Christina M. Kraml, Istvan Pelczer, Thomas Ferrante, Bonnie L. Bassler
Summary: Quorum sensing is a communication process used by bacteria to coordinate collective behaviors through the production, release, and detection of autoinducers. A new study shows that mammalian cells and yeast Saccharomyces cerevisiae can produce an AI-2 mimic called MHF, which can facilitate interdomain signaling through the protein Cff1p. This discovery expands the scope of organisms and molecules involved in quorum sensing interactions.
Article
Biology
Anjali Sharma, Meeta Lavania, Raghvendar Singh, Banwari Lal
Summary: The study isolated a total of 80 presumed lactic acid bacteria from camel milk, which showed potential for producing functional foods and industrial curd formulations. The selected LAB exhibited strong tolerance, antimicrobial activity, adhesion potential, and were non-toxic for industrial applications, making them valuable substitutes in various food and feed industries.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Engineering, Environmental
Yirong Liu, Huizhi Hu, Feng Luo
Summary: The AI-2 mediated QS system has the potential to regulate bacterial behavior and is promising for real-world applications in different WWT systems.
WATER SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Huan Chen, Cheng-Hai Yan, Yu-Fan Zhan, Li-Tian Geng, Lin-Lin Zhu, Lu-Chan Gong, Jun Wang
Summary: Boron derivatives promote biofilm formation in E. coli by enhancing AI-2 activity, increasing EPS secretion, and upregulating the expression levels of biofilm-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Allergy
Mi Kyeong Lee, Annah B. Wyss, Megan U. Carnes, Marie Richards, Christine G. Parks, Laura E. Beane Freeman, Peter S. Thorne, David M. Umbach, M. Andrea Azcarate-Peril, Shyamal D. Peddada, Stephanie J. London
Summary: The study found that the microbial composition of house dust may influence allergic outcomes in adults. The diversity of bacterial communities in house dust may differ by asthma, atopy, or hay fever status.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Immunology
Johanna M. Smeekens, Brandi T. Johnson-Weaver, Andrew L. Hinton, M. Andrea Azcarate-Peril, Timothy P. Moran, Robert M. Immormino, Janelle R. Kesselring, Erin C. Steinbach, Kelly A. Orgel, Herman F. Staats, A. Wesley Burks, Peter J. Mucha, Martin T. Ferris, Michael D. Kulis
Summary: The study found that CC027/GeniUnc mice developed antigen-specific IgE responses to peanuts, walnuts, and eggs, but not milk, while C3H/HeJ mice were not sensitized to any antigen. Sensitized CC027/GeniUnc mice showed significant differences in gut microbiome composition and numbers of immune cells. These results suggest that there are multiple factors associated with enteric sensitization, including decreased fecal IgA levels, increased allergen absorption, and altered gut microbiome composition.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Nutrition & Dietetics
Jason W. Arnold, Hunter D. Whittington, Suzanne F. Dagher, Jeffery Roach, M. Andrea Azcarate-Peril, Jose M. Bruno-Barcena
Summary: GOS, resistant to digestion, selectively stimulates microbial growth in the colon. A novel enzymatic method for generating humanized GOS has been developed, showing the ability to modulate the gut microbiome in vivo, promoting the growth of beneficial microorganisms.
FRONTIERS IN NUTRITION
(2021)
Article
Multidisciplinary Sciences
Johanna R. Jahnke, Jeffrey Roach, M. Andrea Azcarate-Peril, Amanda L. Thompson
Summary: Early life exposure to adverse environments, particularly maternal stress, has been linked to increased risk for metabolic diseases and neurobehavioral disorders. This study examined the relationships among maternal precarity, HPA axis dysregulation, infant gut microbiome composition, and infant HPA axis functioning. The findings suggest that maternal precarity and HPA axis dysregulation are associated with changes in infant gut microbiome composition and HPA axis functioning.
Article
Multidisciplinary Sciences
Alexander L. Carlson, Kai Xia, M. Andrea Azcarate-Peril, Samuel P. Rosin, Jason P. Fine, Wancen Mu, Jared B. Zopp, Mary C. Kimmel, Martin A. Styner, Amanda L. Thompson, Cathi B. Propper, Rebecca C. Knickmeyer
Summary: Experimental manipulation of the gut microbiome in animal models impacts fear behaviors. This pilot study suggests associations between features of the human infant gut microbiome and non-social fear behaviors. Understanding the role of the gut microbiome in human fear behavior development requires further validation with a larger sample size.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Shan Sun, Xiangzhu Zhu, Xiang Huang, Harvey J. Murff, Reid M. Ness, Douglas L. Seidner, Alicia A. Sorgen, Ivory C. Blakley, Chang Yu, Qi Dai, M. Andrea Azcarate-Peril, Martha J. Shrubsole, Anthony A. Fodor
Summary: The study reveals differences in microbial communities among stool, rectal swab, and rectal mucosal tissue samples, and the inference of host phenotypes varies when using different sample types. Inference using stool or rectal swab generally gives significantly correlated results, while inference using mucosal tissue samples is generally less consistent with stool or swab.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Lucia Guadamuro, M. Andrea Azcarate-Peril, Rafael Tojo, Baltasar Mayo, Susana Delgado
Summary: Isoflavones from soy can modulate the gut microbiota composition and activity in menopausal women. This study found that supplementation with isoflavones led to changes in fecal microbiota, with an increase in certain microbial populations in equol-producing women. The results suggest a potential beneficial health effect of isoflavones through modulation of the gut microbiota.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Rheumatology
Richard F. Loeser, Liubov Arbeeva, Kathryn Kelley, Anthony A. Fodor, Shan Sun, Veronica Ulici, Lara Longobardi, Yang Cui, Delisha A. Stewart, Susan J. Sumner, M. Andrea Azcarate-Peril, R. Balfour Sartor, Ian M. Carroll, Jordan B. Renner, Joanne M. Jordan, Amanda E. Nelson
Summary: This study investigated the role of altered gut microbiota in obesity-associated osteoarthritis (OA). The results showed no differences in gut microbiota composition between patients and controls, but increased serum lipopolysaccharide (LPS) levels in patients. This suggests that increased intestinal permeability may contribute to the development of obesity-associated OA.
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Nutrition & Dietetics
Victoria Sanborn, Mashael Aljumaah, M. Andrea Azcarate-Peril, John Gunstad
Summary: The prevalence of dementia is expected to increase with the growing older adult population, and prevention strategies are urgently needed. Physical activity (PA) and probiotic supplementation are two promising interventions, with combined use potentially providing greater cognitive benefits. However, this study found that probiotic supplementation may not improve cognitive function in healthy, physically active older adults.
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM
(2022)
Article
Veterinary Sciences
Tanner S. Slead, Benjamin J. Callahan, Megan E. Schreeg, Gabriela S. Seiler, Devorah M. Stowe, Maria Andrea Azcarate-Peril, Megan E. Jacob, Jody L. Gookin
Summary: This study aimed to determine if gallbladder bile in apparently healthy cats contains a core microbiota composed of bacterial taxa common to many individuals and compare the results of bile cytology, bile culture, and 16S rRNA gene amplicon sequencing in healthy cats and cats with suspected hepatobiliary disease.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2023)
Meeting Abstract
Cell Biology
M. Andrea Azcarate-Peril
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2021)
Meeting Abstract
Oncology
Lei Fan, Danxia Yu, Xiangzhu Zhu, Xuehong Zhang, Xiang Huang, Harvey J. Murff, M. Andrea Azcarate-Peril, Martha J. Shrubsole, Qi Dai
Article
Gastroenterology & Hepatology
M. A. Azcarate-Peril, J. Roach, A. Marsh, William D. Chey, William J. Sandborn, Andrew J. Ritter, Dennis A. Savaiano, T. R. Klaenhammer
Summary: The study confirmed the significant symptom reduction and alteration of fecal microbiome in lactose intolerant patients with the administration of pure galacto-oligosaccharides. The analysis further suggested long-term benefits when individuals continued consumption of dairy products after the treatment.
Article
Microbiology
Thamirys da Costa Rosa, Aline de Almeida Neves, M. Andrea Azcarate-Peril, Kimon Divaris, Di Wu, Hunyong Cho, Kevin Moss, Bruce J. Paster, Tsute Chen, Liana B. Freitas-Fernandes, Tatiana K. S. Fidalgo, Ricardo Tadeu Lopes, Ana Paula Valente, Roland R. Arnold, Apoena de Aguiar Ribeiro
Summary: This experimental study investigated longitudinal changes in bacterial microbiome and metabolome associated with enamel caries lesion progression and arrest. The results revealed the association of specific microbes and metabolites with caries progression and arrest, providing new insights into the role of biofilm in caries development. These findings offer promising candidate biomarkers for future validation studies.
JOURNAL OF ORAL MICROBIOLOGY
(2021)
Meeting Abstract
Neurosciences
Mary Kimmel, Shirin Ataei Kachouei, Karen Grewen, Andrea Azcarate-Peril, Alkistis Skalkidou, Emma Fransson, Janet Cunningham, Samantha Meltzer-Brody
BIOLOGICAL PSYCHIATRY
(2021)
