Article
Chemistry, Medicinal
Emily E. Williford, Caitlin M. DeAngelo, Kevin S. Blake, Hirdesh Kumar, Kendrick K. Lam, Katherine V. Jones, Niraj H. Tolia, Gautam Dantas, Timothy A. Wencewicz
Summary: Tetracyclines (TCs) are a group of important antibiotics that are facing a new resistance mechanism called enzymatic inactivation. To overcome this resistance, combination therapies using TDase inhibitors and TC antibiotics are considered effective. In this study, we designed, synthesized, and evaluated bifunctional TDase inhibitors derived from anhydrotetracycline (aTC). The bisubstrate inhibitors showed enhanced interactions with TDases and blocked TC binding and the reduction of FAD by NADPH, thereby inhibiting the TDases.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Environmental Sciences
Zhong Liu, Huiyun Zou, Zouran Lan, Xuewen Li
Summary: Antibiotic pollution from animal breeding is a serious issue that may lead to environmental antibiotic resistances, posing a threat to human health. A study in Shandong Province, China found prevalent antibiotic contamination in various environmental mediums on animal farms, with tetracyclines exhibiting higher concentrations. 11 antibiotics were prioritized for control, with a shift in focus needed towards extra antibiotic addition in animal breeding processes for local antibiotic management.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Immunology
Christine Roubert, Evelyne Fontaine, Anna M. Upton
Summary: Despite efforts in TB drug discovery, few drugs have shown clear utility against drug resistant TB. Recent developments have allowed for re-evaluation of previously discovered compounds and targets, leading to new possibilities for treatment. These efforts are complemented by research on new chemical matter and targets, enhancing the chances of discovering effective regimens against drug-resistant TB.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Hannah K. Lembke, Erin E. Carlson
Summary: Bacteria form complex communities in our bodies, with most being beneficial but a small percentage being pathogenic. Immediate action is needed to combat the development of antibiotic-resistant bacterial strains, as current therapeutics are not effective. Activity-based probes (ABPs) are a method to investigate proteins in pathogens and can help identify new drug targets or visualize antibiotic resistance and bacterial virulence-associated proteins. Strategies to determine the specificity of ABPs within a targeted enzyme class are also introduced.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Article
Immunology
Li Wang, Michael A. Crackower, Hao Wu
Summary: Inflammasome proteins are important drug targets for many diseases, but their drug discovery has been difficult due to challenges in solving high-resolution structures. Recent advances in the structural biology of NLRP3 and NLRP1 offer hope for structure-based drug design.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Environmental Sciences
Fuyang Huang, Linpeng Chen, Chong Zhang, Fei Liu, Hui Li
Summary: A new method was established to rank priority antibiotics, identifying 26 high priority antibiotics for immediate attention, primarily used in animal husbandry in China. Urgent action is needed to limit the use of these top priority antibiotics in the animal industry.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Physical
Jiayi Wang, Haoxiang Xu, Chunxia Che, Jiqin Zhu, Daojian Cheng
Summary: Researchers have revealed the relationship between surface structure and catalytic performance of PdAg bimetal catalysts using density functional theory calculations and microkinetic models. They have also developed a screening strategy based on structure descriptors to design the surface structure of Pd-based catalysts.
Article
Biochemical Research Methods
Xiang Li, Xu Liu, Ziqing Yu, Yang Luo, Qixin Hu, Zhenye Xu, Jia Dai, Nannan Wu, Feng Shen
Summary: Bacterial antimicrobial resistance is a global threat, and a rapid high-throughput phenotypic antimicrobial susceptibility testing (AST) method is needed. The cs-SlipChip, containing 192 nanoliter-sized compartments, allows for high-throughput AST by monitoring bacterial growth and analyzing changes in bright-field imaging. The chip can measure the minimum inhibitory concentration (MIC) of Escherichia coli against four antibiotics within 3 hours and has been validated against a VITEK 2 automated system. This technology provides a practical and rapid method for AST and screening antibiotic combinations for treating multiple antibiotic-resistant bacteria.
Article
Microbiology
Lea Weber, Anna Hagemann, Jila Kaltenhaeuser, Manuela Besser, Patrick Rockenfeller, Anja Ehrhardt, Ewa Stuermer, Hagen Sjard Bachmann
Summary: FTIs have been widely used in the treatment of various diseases, and studies have shown their potential effectiveness in the treatment of infectious diseases. The impact of FTIs on common human pathogens, especially on the geranyltranstransferase IspA, remains largely unknown.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Hitesh K. Sharma, Puneet Gupta, Dheeraj Nagpal, Monalisa Mukherjee, Virinder S. Parmar, Viney Lather
Summary: Staphylococcus aureus is a severe bacterial pathogen with widespread antibiotic resistance. This study identified potential molecules with antibacterial activity, particularly curcumin which demonstrated the lowest MIC values, highlighting its exceptional antibacterial activity, especially in combination with amoxicillin.
Article
Environmental Sciences
Feifei Li, Yingyu Bao, Lyujun Chen, Zhiguo Su, Yushi Tang, Donghui Wen
Summary: Antibiotics as emerging pollutants have detrimental effects on organisms in the environment. A comprehensive PBTR framework that incorporates resistance risk with persistence, bioaccumulation, and toxicity was established to assess antibiotic-specific risk. The study evaluated 74 antibiotics in Chinese seawater and provided a feasible screening strategy and monitoring recommendations for priority antibiotics.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Nanoscience & Nanotechnology
Sohini Basu Roy, Ahmed Nabawy, Aritra Nath Chattopadhyay, Yingying Geng, Jessa Marie Makabenta, Akash Gupta, Vincent M. Rotello
Summary: Antibiotic resistance is a critical threat to public health. Researchers have developed a high-throughput polymer-based sensor platform that can rapidly analyze the mechanisms of antibiotic activity and differentiate different generations of antibiotic resistance. This platform is trainable, simple, and allows for rapid screening, making it potentially suitable for practical applications.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Sohini Basu Roy, Ahmed Nabawy, Aritra Nath Chattopadhyay, Yingying Geng, Jessa Marie Makabenta, Akash Gupta, Vincent M. Rotello
Summary: Antibiotic resistance poses a threat to public health and requires the rapid development of new antibiotics and appropriate treatment methods to combat refractory bacterial infections. This study reports a high-throughput polymer-based sensor platform that can rapidly analyze antibiotic activity mechanisms and effectively discriminate different generations of antibiotic resistance.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
Cathrin Hauk, Mark Boss, Julia Gabel, Simon Schaefermann, Hendrik P. A. Lensch, Lutz Heide
Summary: Substandard and falsified medicines can be identified using a low-cost smartphone-based imaging software for thin-layer chromatography analysis. The performance evaluation of the tool shows its accuracy, precision, and robustness in analyzing active pharmaceutical ingredients. A locally producible wooden box is designed to ensure standardized TLC photography under controlled lighting conditions.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Rebecca L. Mattison, Roland Beffa, Guido Bojack, Birgit Bollenbach-Wahl, Christine Doernbrack, Nicole Dorn, Joerg Freigang, Elmar Gatzweiler, Rahel Getachew, Claudia Hartfiel, Ines Heinemann, Hendrik Helmke, Sabine Hohmann, Harald Jakobi, Gudrun Lange, Peter Luemmen, Lothar Willms, Jens Frackenpohl
Summary: This research reports a series of novel small molecular compounds with good herbicidal activity, which can effectively control weeds in corn and rice crops. Some of these compounds are more efficient than recently commercialized related herbicides. The results suggest that these compounds have the potential to be developed as new PPO-inhibitor herbicides to combat weed resistance to current market standards.
PEST MANAGEMENT SCIENCE
(2023)