Article
Biochemistry & Molecular Biology
Romain Durand, Kevin T. Huguet, Nicolas Rivard, Nicolas Carraro, Sebastien Rodrigue, Vincent Burrus
Summary: IncC conjugative plasmids and the SGI1 family are common mobile genetic elements in Gammaproteobacteria, with SgaCD and AcaCD being key factors in their transfer process, activating promoters driving plasmid transfer functions. SgaCD is essential for eliciting the lifecycle of SGI1, including excision and replication, and destabilizing the helper IncC plasmid.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Xenia Vazquez, Javier Fernandez, Margarita Bances, Pilar Lumbreras, Miriam Alkorta, Silvia Hernaez, Elizabeth Prieto, Pedro de la Iglesia, Maria de Toro, M. Rosario Rodicio, Rosaura Rodicio
Summary: This study characterized all ciprofloxacin-resistant Salmonella enterica serovar Kentucky (S. Kentucky) isolates detected in five Spanish hospitals from 2009 to 2018 through whole genome sequencing. The isolates all belonged to sequence type 198 and exhibited resistances to multiple antibiotics mediated by various resistance genes, with a close genetic relationship observed between isolates from different hospitals.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Khyati Bhardwaj, Suchitra M. Shenoy, Shrikala Baliga, B. Unnikrishnan, B. Shantharam Baliga, Vasanth Kumar Shetty
Summary: The study conducted in poultry farms of coastal Southern Karnataka, India, found high levels of antibiotic resistance in Escherichia coli and Klebsiella pneumoniae isolates, with a focus on resistance to fluoroquinolones and tetracycline. The most common resistance genes detected were qnrB, qnrS, and qnrA, with most isolates showing high MAR indices reflecting widespread antibiotic use.
Review
Genetics & Heredity
Claire de Curraize, Eliane Siebor, Catherine Neuwirth
Summary: Salmonella Genomic Island 1 (SGI1) and its related elements are widely distributed in various bacteria, characterized by integrases belonging to the tyrosine-recombinase family and harboring multiple antibiotic resistance regions with AcaCD binding sites, playing a crucial role in antibiotic resistance.
Article
Biochemistry & Molecular Biology
Istvan Nagy, Monika Szabo, Anna Hegyi, Janos Kiss
Summary: The SGI1-family elements are important vehicles of antibiotic resistance among enteric bacteria, mobilized specifically by IncA and IncC-family plasmids. In addition to the basic mobilization module containing oriT and mobilization proteins MpsA and MpsB, SGI1 also relies on an essential transfer factor called sgm-sRNA, which is a small RNA deriving from the 3'-end of a primary RNA.
MOLECULAR MICROBIOLOGY
(2021)
Article
Infectious Diseases
Juan He, Changwei Lei, Cui Li, Xuechun Wang, Pengfei Cui, Hongning Wang
Summary: This study aimed to determine the genetic environment of antimicrobial resistance genes in Proteus mirabilis strain STP3 isolated from a diarrhoeic pig on a swine farm in Sichuan Province, China. The identification of two genomic islands (GIs) in this study suggested that genetic elements might be key mediators for resistance gene acquisition in P. mirabilis and that IS26-mediated rearrangements promote the diversity of GIs.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Eliane Siebor, Claire de Curraize, Veronique Varin, Arnaud Magallon, Catherine Neuwirth
Summary: This study aimed to investigate the integration of a VEB-1-encoding gene cassette into the integron of genomic islands harbored by Proteus mirabilis strains after antibiotic exposure. The results showed that under ceftazidime pressure, gene cassettes were inserted into the GIs, contributing to the acquisition, maintenance, and spread of antibiotic resistance genes.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
Xing-Wei Luo, Pei-Yi Liu, Qing-Qing Miao, Rong-Jia Han, Hua Wu, Jian-Hua Liu, Dan-Dan He, Gong-Zheng Hu
Summary: Mobile genetic elements (MGEs), especially IS26-bounded translocatable units, can serve as a reservoir for a variety of antimicrobial resistance genes in clinically important pathogenic bacteria. This study investigated a representative M. morganii isolate containing multidrug resistance genes, including the infrequent dfrA24, to provide insights into this significant genetic characteristic. The acquired resistance genes in M. morganii were predominantly carried by important MGEs flanked by IS26, such as the novel Tn7376 and the MMGI-4. The emergence of the extra gene dfrA24 in M. morganii was likely facilitated by various genetic events, including recombination, transposition, and integration mediated by IS26-related MGEs. Therefore, continuous surveillance of these MGEs in clinical pathogens is crucial for assessing the risk of emergence and development of novel resistance genes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jun Kwon, Myoung-Hwan Yang, Hyoung-Joon Ko, Sang-Guen Kim, Chul Park, Se-Chang Park
Summary: This study analyzed the antibiotic resistance pattern of Proteus mirabilis, an emerging pathogen, and found a high resistance-pattern ratio. Prudent and selective use of antibiotics is necessary to prevent antibiotic resistance.
Article
Immunology
Qiang Zhao, Yueyun Shen, Gang Chen, Yanping Luo, Shenghui Cui, Yaping Tian
Summary: The study analyzed the drug resistance prevalence and genetic characteristics of fecal E. coli isolated from healthy children at a kindergarten in Beijing, China. The results showed high resistance to multiple antibiotics and observed clonal spread of certain prevalent sequence types among the isolates. The findings highlight the seriousness of multidrug resistance and potential pathogenicity of E. coli in healthy children, emphasizing the need for increased surveillance and control measures.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Public, Environmental & Occupational Health
Anu Kantele, Tinja Laaveri
Summary: Antibiotic treatment for diarrhoeagenic Escherichia coli (DEC) in travellers' diarrhoea is becoming less effective, with increasing proportions of ESBL-producing DEC and resistance to various antibiotics.
JOURNAL OF TRAVEL MEDICINE
(2022)
Article
Infectious Diseases
Jiayu Shao, Hangdong Dai, Liwei Xu, Shuilong Zhu, Jufang Zhu, Hangyu Fu, Minxia Ge, Xianhong He
Summary: The purpose of this study was to investigate the genomic characteristics and antimicrobial resistance mechanisms of ESBL-producing E. coli isolates recovered from a district hospital in China. The results showed that these isolates exhibited resistance to multiple antibiotics, with the majority belonging to the ST131 serotype and having different virulence genes and sequence types. Therefore, continuous surveillance and control of the transmission of these multi-drug resistant bacteria in clinical and community settings are crucial.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Mustafa Sadek, Jose Manuel Ortiz de la Rosa, Mohamed Abdelfattah Maky, Mohamed Korashe Dandrawy, Patrice Nordmann, Laurent Poirel
Summary: This study identified mcr-1-positive Enterobacterales from retail raw chicken in Egypt, highlighting the diversity and multidrug resistance of these isolates.
Article
Chemistry, Medicinal
Yanshu Jia, Liyan Zhao
Summary: Bacterial infection is a common disease and fluoroquinolones are widely used antibacterial agents. However, the emergence of drug resistance and more virulent pathogens highlights the need for developing new drugs. Research aims to summarize recent advances in fluoroquinolone derivatives and structure-activity relationships.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Infectious Diseases
Lisa Praski Alzrigat, Douglas L. Huseby, Gerrit Brandis, Diarmaid Hughes
Summary: Experimental evolution did not identify any single pump-independent cost factor, but efficient fitness compensation occurred only by mechanisms that reduce MarA concentration, simultaneously reducing the drug resistance phenotype. This resistance/fitness trade-off serves as a barrier to the successful spread of MarR inactivation mutations in clinical isolates where growth fitness is essential.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
S. Simeon, V Le Moing, S. Tubiana, X. Duval, D. Fournier, J-P Lavigne, M-L Erpelding, C-A Gustave, S. Desage, C. Chirouze, F. Vandenesch, P. Tattevin, Catherine Chirouze, Elodie Curlier, Cecile Descottes-Genon, Bruno Hoen, Isabelle Patry, Lucie Vettoretti, Pascal Chavanet, Jean-Christophe Eicher, Sandrine Gohier-Treuvelot, Marie-Christine Greusard, Catherine Neuwirth, Andre Pechinot, Lionel Piroth, Marie Celard, Catherine Cornu, Francois Delahaye, Malika Hadid, Pascale Rausch, Audrey Coma, Florence Galtier, Philippe Geraud, Helene Jean-Pierre, Vincent Le Moing, Catherine Sportouch, Jacques Reynes, Nejla Aissa, Thanh Doco-Lecompte, Francois Goehringer, Nathalie Keil, Lorraine Letranchant, Hepher Malela, Thierry May, Christine Selton-Suty, Nathalie Bedos, Jean-Philippe Lavigne, Catherine Lechiche, Albert Sotto, Xavier Duval, Emila Ilic Habensus, Bernard Iung, Catherine Leport, Pascale Longuet, Raymond Ruimy, Eric Bellissant, Pierre Yves Donnio, Fabienne Le Gac, Christian Michelet, Matthieu Revest, Pierre Tattevin, Elise Thebault, Francois Alla, Pierre Braquet, Marie-Line Erpelding, Laetitia Minary, Sarah Tubiana, Michele Bes, Jerome Etienne, Taissia Lelekov-Boissard, Anne Tristan, Francois Vandenesch, Alex Van Belkum, Fernando Rivadeneira, Willem Vanwamel, Sandrine Barbas, Christine Delonca, Virginie Sussmuth, Anne Verchere
CLINICAL MICROBIOLOGY AND INFECTION
(2019)
Article
Infectious Diseases
Eliane Siebor, Claire de Curraize, Catherine Neuwirth
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Microbiology
Diane Pivot, Annlyse Fanton, Edgar Badell-Ocando, Marion Benouachkou, Karine Astruc, Frederic Huet, Lucie Amoureux, Catherine Neuwirth, Alexis Criscuolo, Serge Aho, Julie Toubiana, Sylvain Brisse
JOURNAL OF CLINICAL MICROBIOLOGY
(2019)
Article
Respiratory System
Lucie Amoureux, Juliette Sauge, Benoit Sarret, Matthieu Lhoumeau, Audrey Bajard, Jennifer Tetu, Julien Bador, Catherine Neuwirth, Jocelyne Caillon, Emilie Cardot-Martin, Vincent Cattoir, Anne Doleans-Jordheim, Agnes Ferroni, Helene Guet-Revillet, Genevieve Hery-Arnaud, Christine Segonds, Elise Thomas, Patrick Plesiat, Hoang Vu-Thien
JOURNAL OF CYSTIC FIBROSIS
(2019)
Article
Genetics & Heredity
Claire de Curraize, Eliane Siebor, Catherine Neuwirth, Ruth M. Hall
Article
Microbiology
Claire de Curraize, Eliane Siebor, Veronique Varin, Catherine Neuwirth, Ruth M. Hall
Correction
Microbiology
Claire de Curraize, Eliane Siebor, Veronique Varin, Catherine Neuwirth, Ruth M. Hall
Article
Infectious Diseases
Eliane Siebor, Catherine Neuwirth
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2020)
Review
Genetics & Heredity
Claire de Curraize, Eliane Siebor, Catherine Neuwirth
Summary: Salmonella Genomic Island 1 (SGI1) and its related elements are widely distributed in various bacteria, characterized by integrases belonging to the tyrosine-recombinase family and harboring multiple antibiotic resistance regions with AcaCD binding sites, playing a crucial role in antibiotic resistance.
Article
Infectious Diseases
Thomas Maldiney, Dorian Bonnot, Nelson Anzala, Sandrine Albac, Delphine Labrousse, Emmanuelle Varon, Lucie Amoureux, Angelique Chapuis, Julien Bador, Catherine Neuwirth, Delphine Croisier, Pascal Chavanet
Summary: The study aimed to evaluate the in vitro antimicrobial activity of daptomycin-based combinations against bacterial meningitis pathogens and found that the adjunctive use of daptomycin did not impact the antimicrobial activity of amoxicillin, cefotaxime, or rifampicin. For Gram-positive bacteria, the addition of daptomycin enhanced growth inhibition and bactericidal activity, particularly demonstrating a synergistic effect against Listeria monocytogenes.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Arnaud Magallon, Lucie Amoureux, Thomas Garrigos, Marine Sonois, Veronique Varin, Catherine Neuwirth, Julien Bador
Summary: The AxyABM efflux system plays a significant role in the acquired resistance of Achromobacter to commonly used antibiotics in CF patients, including ciprofloxacin, meropenem, and ceftazidime. Overproduction of AxyABM may also lead to increased minimum inhibitory concentration of the new promising antibiotic cefiderocol.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Immunology
Emmanuelle Gras, Emmanuelle Bergeron, Mathilde Puges, Mailys Ducours, Charlotte Leleux, Lucie Amoureux, Baptiste Jean, Imane Bendjelloul, Francois Camelena, Rachel Chenouard, Rafael Mahieu, Olivier Lemenand, Alexandre Toro, Alain Lecoustumier, Olivier Lortholary, Veronica Rodriguez Nava, David Lebeaux
Summary: This study investigated the clinical significance of Streptomyces isolates in human samples and the characteristics of patients with invasive Streptomyces infections. The findings showed that half of the clinical samples came from patients with invasive infections, and the recommended antimicrobial therapy included linezolid, amikacin, or imipenem.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Arnaud Magallon, Mathilde Roussel, Catherine Neuwirth, Jennifer Tetu, Anne-Charlotte Cheiakh, Baptiste Boulet, Veronique Varin, Victor Urbain, Julien Bador, Lucie Amoureux
Summary: In Achromobacter, fluoroquinolone resistance is primarily due to overproduction of the RND efflux system AxyEF-OprN, rather than target alteration. This was demonstrated in one in vitro mutant.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Letter
Immunology
Lucie Amoureux, Julien Bador, Catherine Neuwirth
EMERGING INFECTIOUS DISEASES
(2019)
Article
Medicine, General & Internal
Clement Auvens, Catherine Neuwirth, Lionel Piroth, Mathieu Blot