Article
Microbiology
Dessislava Staneva, Bela Vasileva, Petar Podlesniy, George Miloshev, Milena Georgieva
Summary: Mitochondria are important for various cellular functions and their regulation involves nuclear-encoded proteins and chromatin structure. This study investigated the impact of chromatin mutants on mtDNA copy number and mitochondrial membrane potential in Saccharomyces cerevisiae. The mutants showed strain-specific changes in mtDNA copy number, disrupted mitochondrial membrane potential, altered expression of nuclear genes involved in mitochondria regulation, and premature ageing phenotype, particularly in the double mutant arp4 hho1A.
Article
Pharmacology & Pharmacy
Huan Yang, Wanda van der Stel, Randy Lee, Caroline Bauch, Sam Bevan, Paul Walker, Bob van de Water, Erik H. J. Danen, Joost B. Beltman
Summary: Mitochondria are the main bioenergetic organelles of cells and exposure to chemicals targeting mitochondria generally results in toxicity. Different mitochondrial respiration inhibitors have varying effects on mitochondrial membrane potential, and mathematical modeling can help understand these dynamics. Additionally, incorporating pharmacokinetic decay into the model can improve the fit for certain compounds, showing a complex interplay between pharmacokinetics and MMP dynamics within mitochondria.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Siqi Liu, Shanshan Liu, Baiyu He, Lanlan Li, Lin Li, Jiawen Wang, Tao Cai, She Chen, Hui Jiang
Summary: The research reveals that yeast mutants deficient in certain oxidative phosphorylation complexes and mtDNA exhibit progressive reduction of mitochondrial membrane potential. These mutants activate adaptive responses, including remodeling of mitochondrial proteome and reshaping of cellular metabolism.
Review
Biochemistry & Molecular Biology
Hagai Rottenberg
Summary: It is widely reported that the mitochondrial membrane potential, increment ψm, is reduced in aging animals. It was recently suggested that the lower increment ψm in aged animals modulates mitochondrial bioenergetics and that this effect is a major cause of aging since artificially increased increment ψm in C. elegans increased lifespan. The activation of the voltage-gated mitochondrial permeability transition pore (mPTP) is the main cause of the increase in depolarized mitochondria in aged cells, inhibiting oxidative phosphorylation, releasing calcium and mROS, and depleting NAD(+), thereby accelerating degenerative diseases and aging. The reported lifespan extension by artificially generated increment ψm in C. elegans is best explained by inhibition of the voltage-gated mPTP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Amandine Rovini, Kareem Heslop, Elizabeth G. Hunt, Morgan E. Morris, Diana Fang, Monika Gooz, Akos A. Gerencser, Eduardo N. Maldonado
Summary: The study quantified intercellular differences in Delta psi m in human cancer cells and fibroblasts, showing that Delta psi m is more heterogeneous in cancer cells and maintained throughout the cell cycle. The effect of chemical inhibition of the respiratory chain and ATP synthesis varied between cells with different levels of Delta psi m.
Article
Radiology, Nuclear Medicine & Medical Imaging
Matthieu Pelletier-Galarneau, Yoann Petibon, Chao Ma, Paul Han, Sally Ji Who Kim, Felicitas J. Detmer, Daniel Yokell, Nicolas Guehl, Marc Normandin, Georges El Fakhri, Nathaniel M. Alpert
Summary: This study presents the first in vivo, non-invasive assessment of mitochondrial membrane potential in the myocardium of normal human subjects. The results showed excellent agreement with previous in vitro assessments. In vivo quantification of mitochondrial function may provide new diagnostic and prognostic information for cardiac diseases, as well as monitoring therapy.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2021)
Article
Toxicology
David S. Umbaugh, Nga T. Nguyen, Hartmut Jaeschke, Anup Ramachandran
Summary: Mitochondrial morphology changes play a critical role in acetaminophen (APAP) hepatotoxicity, with distinct morphologies corresponding to differences in mitochondrial respiratory function and polarization. These changes in morphology are dose-dependent and can be reversible or irreversible depending on the severity of APAP overdose.
TOXICOLOGICAL SCIENCES
(2021)
Article
Medicine, Research & Experimental
Yoshikazu Kuwahara, Kazuo Tomita, Mehryar Habibi Roudkenar, Amaneh Mohammadi Roushandeh, Yusuke Urushihara, Kento Igarashi, Akihiro Kurimasa, Tomoaki Sato
Summary: The study revealed that modulation of mitochondrial function is implicated in the radioresistance phenotype of cancer cells, and radioresistance of clinically relevant radioresistant cells is reversible. Further studies are needed to clarify the mechanisms underlying cancer cell radioresistance.
Article
Biochemistry & Molecular Biology
Nikita G. Nikiforov, Anastasia Ryabova, Marina V. Kubekina, Igor D. Romanishkin, Kirill A. Trofimov, Yegor S. Chegodaev, Ekaterina Ivanova, Alexander N. Orekhov
Summary: This study found changes in mitochondrial function in monocytes of patients with atherosclerosis, which were associated with chronic inflammation and atherosclerosis development.
Article
Chemistry, Multidisciplinary
Bo Lin, Yunfan Liu, Xiaoping Zhang, Li Fan, Yang Shu, Jianhua Wang
Summary: The study introduces a novel fluorescent probe TPE-NT for imaging Delta Psi(m) in live cells, providing valuable information about cell health and injury levels. The probe effectively eliminates false positive signals on non-mitochondria, exhibiting excellent photostability and low cytotoxicity for real-time imaging applications in various cell models of damage and disease.
Article
Andrology
Zohreh Talebi-Yazdabadi, Neda Jahanbakhsh, Kianoush Dormiani, Mahboobeh Forouzanfar, Liana Lachinani, Dina Zohrabi, Marziyeh Tavalaee, Mohammad Hossein Nasr-Esfahani
Summary: This study assessed sperm MSI1 and MSI2, as well as sperm functional tests in infertile men with varicocele and fertile men. While varicocele was associated with lower sperm concentration and motility, and higher abnormal sperm morphology, lipid peroxidation, DNA fragmentation and protamine deficiency, the expression of MSI1 and MSI2 genes in sperm cells was not significantly affected. The findings suggest that varicocele does not impact the relative expression of these genes in spermatozoa, despite their important roles in spermatogenesis.
Article
Biochemistry & Molecular Biology
Reut Hazan (Ben-Menachem), Dvora Lintzer, Tamar Ziv, Koyeli Das, Irit Rosenhek-Goldian, Ziv Porat, Hila Ben Ami Pilo, Sharon Karniely, Ann Saada, Neta Regev-Rudzki, Ophry Pines
Summary: Vesicular transport is a means of communication between cells, and this study focuses on organelle-to-organelle communication, specifically between mitochondria. The researchers discovered that functional mitochondria in yeast can release vesicles, which are about 100 nm in size and carry selective protein cargo, including ATP synthase subunits. These vesicles also contain a functional ATP synthase complex and have the ability to fuse with naive mitochondria, potentially delivering ATP and participating in organelle-to-organelle communication.
Article
Chemistry, Analytical
Jie Niu, Shuxian Yang, Minggang Tian, Xiaoqiang Yu
Summary: This study proposes a new strategy to alter the fluorescence lifetime of probes by changing their subcellular immigration and develops two viscosity-responsive isomeric probes (PQO and PQP) for monitoring changes in mitochondrial membrane potential (ΔΨm) under fluorescence lifetime imaging microscopy. The probes exhibit different fluorescence lifetime in cells with high and low levels of ΔΨm, enabling visualization of ΔΨm.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Andrology
Mohamad Reza Sadraei, Marziyeh Tavalaee, Mohsen Forouzanfar, Mohammad Hossein Nasr-Esfahani
Summary: The study found that both curcumin and nano-curcumin as natural antioxidants had significant effects on spermatogenesis and sperm function in varicocelised rats, with nano-curcumin showing more effectiveness. This suggests that nano-curcumin may be a preferable option for treating infertility in individuals with varicocele.
Article
Obstetrics & Gynecology
Francesco Ragonese, Lorenzo Monarca, Antonella De Luca, Loretta Mancinelli, Monica Mariani, Cristina Corbucci, Sandro Gerli, Rossana Giulietta Iannitti, Lucio Leonardi, Bernard Fioretti
Summary: The study showed that resveratrol can enhance mitochondrial activity and cell viability in human granulosa cells, while reducing potassium current, leading to an increase in intracellular Ca2? levels. These findings suggest potential therapeutic implications of resveratrol in improving female reproductive system physiology.
FERTILITY AND STERILITY
(2021)
Article
Medicine, General & Internal
Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero, Rossella Cannarella, Antonio Aversa
Summary: This study prospectively investigates the effects of liraglutide, a GLP1 analog, on reproductive and sexual function in men with metabolic hypogonadism. The results show that liraglutide significantly improves sperm parameters and erectile function, as well as increasing testosterone and sex hormone-binding globulin levels. This suggests that liraglutide is a useful pharmacological treatment for male patients with metabolic hypogonadism.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Claudio Manna, Federica Barbagallo, Francesca Sagnella, Ashraf Farrag, Aldo E. E. Calogero
Summary: The commonly used protocols in assisted reproductive technology (ART) involve long-term embryo culture up to the blastocyst stage, freezing of all embryos, and subsequent transfer one by one. These practices have the potential risks of obstetric and perinatal complications, adverse epigenetic consequences, and ethical-legal concerns regarding frozen embryo storage. To minimize risks, the study suggests selecting a limited number of oocytes for insemination and transferring embryos at the cleaving stage. The results showed a comparable live birth rate to the commonly used protocols in older women with reduced ovarian reserve.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Andrology
Andrea Crafa, Rosita Angela Condorelli, Sandro La Vignera, Aldo Eugenio Calogero, Rossella Cannarella
Summary: This article presents a clinical case of a globozoospermic patient with a new mutation of the DPY19L2 gene and provides recommendations based on a systematic literature review on gene mutations, assisted reproductive techniques, and the risk of genetic abnormalities transmission. The study highlights the importance of genetic evaluation and specific assisted reproductive techniques in managing globozoospermia patients.
WORLD JOURNAL OF MENS HEALTH
(2023)
Review
Andrology
Aldo E. Calogero, Rossella Cannarella, Ashok Agarwal, Taha Abo-Almagd Abdel-Meguid Hamoda, Amarnath Rambhatla, Ramadan Saleh, Florence Boitrelle, Imad Ziouziou, Tuncay Toprak, Murat Gul, Tomer Avidor-Reiss, Parviz Kavoussi, Eric Chung, Ponco Birowo, Ramy Abou Ghayda, Edmund Ko, Giovanni Colpi, Fotios Dimitriadis, Giorgio Ivan Russo, Marlon Martinez, Gokhan Calik, Hussein Kandil, Gianmaria Salvio, Taymour Mostafa, Haocheng Lin, Hyun Jun Park, Nazim Gherabi, Nguyen Ho Vinh Phuoc, Nguyen Quang, Ricky Adriansjah, Sandro La Vignera, Sava Micic, Damayanthi Durairajanayagam, Ege Can Serefoglu, Vilvapathy Senguttuvan Karthikeyan, Priyank Kothari, Widi Atmoko, Rupin Shah
Summary: Infertility affects nearly 186 million people worldwide, with male infertility accounting for about half of the cases. There has been a decline in sperm concentration and total count over the past four decades, emphasizing the need for further research in Andrology.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Medicine, General & Internal
Livia Basile, Rosita A. Condorelli, Aldo E. Calogero, Rossella Cannarella, Federica Barbagallo, Andrea Crafa, Antonio Aversa, Sandro La Vignera
Summary: Red wine is rich in nutrients and has been the focus of numerous scientific studies. It has been reported that moderate consumption of red wine, with its phenolic and antioxidant properties, can have positive effects on various diseases. Additionally, the ethanol content in red wine has shown various biological properties. However, little is known about the correlation between red wine consumption and male sexual function. This review aimed to evaluate the effects of moderate red wine consumption on erectile function and found potential benefits in patients with erectile dysfunction as well as positive influences on reproductive function through vasorelaxant and antioxidant mechanisms.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Francesca Privitera, Danilo Centonze, Sandro La Vignera, Rosita Angela Condorelli, Costanza Distefano, Rossella Gioco, Alba Ilari Civit, Giordana Riccioli, Ludovica Stella, Andrea Cavallaro, Matteo Angelo Cannizzaro, Massimiliano Veroux
Summary: This study evaluated the incidence and potential risk factors for postoperative hypoparathyroidism after thyroid surgical procedures in a single high-volume center. The results showed that young patients undergoing neck dissection and with incidental parathyroidectomy have the highest risk of postoperative hypoparathyroidism.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Andrea Crafa, Rossella Cannarella, Federica Barbagallo, Claudia Leanza, Roberto Palazzolo, Hunter Ausley Flores, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: Vitamin D deficiency and erectile dysfunction are common in males, especially in the elderly, suggesting a possible relationship between the two conditions. Studies indicate that vitamin D affects erectile function by regulating endothelial function and interfering with comorbidities associated with erectile dysfunction. Low levels of vitamin D may worsen erectile function through various mechanisms. However, the quality and heterogeneity of clinical trials evaluating the effects of vitamin D on erectile function and related comorbidities require further investigation.
Review
Biochemistry & Molecular Biology
Federica Barbagallo, Rossella Cannarella, Andrea Crafa, Sandro La Vignera, Rosita A. Condorelli, Claudio Manna, Aldo E. Calogero
Summary: This systematic review and meta-analysis found that a very short abstinence period (within 4 h) has a positive effect on assisted reproductive technique (ART) outcomes, significantly increasing the implantation rate, clinical pregnancy rate, and live birth rate. However, it does not have a significant impact on fertilization rate and miscarriage rate. It suggests that a second ejaculation shortly after the first one can be a simple strategy to improve ART outcomes, particularly in couples involving patients with abnormal sperm parameters.
Review
Pharmacology & Pharmacy
Rossella Cannarella, Cristina M. B. Petralia, Rosita A. Condorelli, Antonio Aversa, Aldo E. Calogero, Sandro La Vignera
Summary: This article provides an overview of the molecular mechanisms and usage of follicle-stimulating hormone (FSH) in the treatment of male infertility. It also evaluates the predictor factors of FSH administration response and the indications of the latest guidelines on the use of FSH in male infertility treatment.
EXPERT OPINION ON INVESTIGATIONAL DRUGS
(2023)
Article
Biochemistry & Molecular Biology
Alyssa J. J. Paganoni, Rossella Cannarella, Roberto Oleari, Federica Amoruso, Renata Antal, Marco Ruzza, Chiara Olivieri, Rosita A. Condorelli, Sandro La Vignera, Fationa Tolaj, Anna Cariboni, Aldo E. Calogero, Paolo Magni
Summary: Gonadotropin-releasing hormone (GnRH) neurons play a crucial role in regulating reproduction. AMH, GH, and IGF1 were found to enhance the migration and function of GnRH neurons in vitro. However, their involvement in the development and function of GnRH neurons in vivo remains unknown. This study evaluated the expression of AMHR2, GHR, and IGF1R during different developmental stages in mice, revealing differential protein expression patterns.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Andrology
Rossella Cannarella, Marta Marino, Rosita A. Condorelli, Sandro La Vignera, Aldo E. Calogero
Summary: Epidemiologists emphasize that male factors account for about half of couple infertility cases, yet the importance of andrologists or endocrinologists in ART centers is undervalued. A lack of comprehensive clinical evaluation for male partners, often limited to semen analysis, performed by laboratory technicians, leads to missed opportunities for identifying treatable causes, recognizing diseases requiring immediate treatment, and detecting genetic diseases in offspring. Integrating medical personnel with expertise in andrology and endocrinology in ART centers is the first step towards precision medicine. It can enhance the success rate of ART, reducing psychological and financial burdens on public health resources and infertile couples. Guideline of scientific societies should acknowledge this possibility.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Sabrina Bossio, Anna Perri, Raffaella Gallo, Anna De Bartolo, Vittoria Rago, Daniele La Russa, Michele Di Dio, Sandro La Vignera, Aldo E. Calogero, Giovanni Vitale, Antonio Aversa
Summary: Alpha-lipoic acid (ALA) has been shown to inhibit cell growth and reduce viability in prostate cancer cells by inhibiting autophagy and affecting the redox balance system. ALA also reduces cell migration and invasion by regulating signaling pathways. These findings contribute to a better understanding of the potential therapeutic effects of ALA in prostate cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Andrology
Rossella Cannarella, Claudia Leanza, Andrea Crafa, Federica Barbagallo, Sandro La Vignera, Rosita Angela Condorelli, Aldo Eugenio Calogero
Summary: This study examined the impact of MEST gene methylation pattern on sperm parameters in infertile patients and found that MEST gene methylation was higher in infertile patients compared to normal men.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Andrology
Rossella Cannarella, Matteo Bertelli, Rosita A. Condorelli, Marija Vilaj, Sandro La Vignera, Davor Jezek, Aldo E. Calogero
Summary: This study revealed that different mutations of the same gene can result in various testicular histological pictures. It suggests that it is the type of gene mutation/variation rather than the gene itself that determines the testicular histology. However, designing a genetic panel with prognostic value for testicular sperm extraction in patients with NOA remains challenging.
WORLD JOURNAL OF MENS HEALTH
(2023)
