Article
Biochemistry & Molecular Biology
Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski, Roger A. Sunde
Summary: By using HPLC and ICP-MS, it has been found that selenium (Se) primarily exists as selenosugar in adequate turkey liver, while in rats, selenium is present as selenocysteine and selenosugar. Selenosugar-decorated proteins become the major Se-containing protein species in the liver with high Se supplementation.
Article
Biochemistry & Molecular Biology
Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski, Roger A. Sunde
Summary: This study found that the metabolism and accumulation of selenomethionine in rats is different from that of selenocysteine. At the selenium requirement for maximal glutathione peroxidase activity, selenocysteine is the major selenium species, while at higher intakes of selenomethionine, it is metabolized into low molecular weight selenosugars and selenosugar-decorated proteins. Additionally, compared to selenite, selenomethionine leads to less binding of inorganic selenium to liver proteins.
Article
Biochemistry & Molecular Biology
Hui Zhu, Katarzyna Bierla, Jun Tan, Joanna Szpunar, Daijie Chen, Ryszard Lobinski
Summary: This study investigated the influence of the fermentation process on selenite metabolism and selenium enrichment in the probiotic Bifidobacterium longum DD98. The study evaluated the effects of sodium selenite concentration, feeding time, and fermentation stage on the distribution of selenium compounds and the speciation of selenium in the metabolite fraction. The results showed that the fermentation process significantly affected the incorporation of selenium into metabolites and proteins in B. longum DD98, offering a tool for optimizing the production of selenium-enriched probiotics with desired molecular speciation.
Article
Agriculture, Multidisciplinary
L. Ferrari, V Sele, M. Silva, P. Bonilauri, F. De Filippo, F. Selmin, R. Ornsrud, L. Pinotti, M. Ottoboni
Summary: This study investigated the accumulation and speciation of selenium in black soldier fly larvae reared on different substrates. The results showed that the larvae were able to convert inorganic selenium to organic selenium, leading to significantly higher selenium levels in the larvae reared on a selenium-fortified diet. This suggests that black soldier fly larvae could be a potential future source of selenium in feed.
JOURNAL OF INSECTS AS FOOD AND FEED
(2022)
Article
Biochemical Research Methods
Martin Braun, Sarka Ryglova, Tomas Suchy
Summary: This study presents a sensitive reversed-phase gradient HPLC method combined with fluorescence detection for quantifying glycosaminoglycans (GAGs) in collagen-based biomaterials. The method allows for the identification and separation of GAGs from other components with a low detection limit, making it suitable for laboratory analysis.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Environmental Sciences
Lin Chen, Shun Kong, Guodong Wang, Xiaoju Yan, Xuemei Zhang, Xiangji Kong, Yuanqing Bu
Summary: This study developed an analytical method using reversed-phase high-performance liquid chromatography to quantify glufosinate-P-ammonium (GLUF-P) in farmland soil. The method showed high sensitivity and could be used for the determination of GLUF-P residues in farmland soil.
Article
Agriculture, Multidisciplinary
Hui Zhu, Katarzyna Bierla, Xueli Jin, Joanna Szpunar, Daijie Chen, Ryszard Lobinski
Summary: This study analyzed the Se metabolites of a Se-enriched probiotic and identified gamma-glutamyl-selenomethionine as the major Se metabolite. It also discovered 11 minor Se metabolites, 8 of which were not previously reported in probiotics. These findings demonstrate the potential of this probiotic as an alternative source of organic Se for food and feed supplementation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemical Research Methods
Taylor Battellino, Kosuke Ogata, Victor Spicer, Yasushi Ishihama, Oleg Krokhin
Summary: In this study, the sensitivity of peptide analysis and chromatographic behavior were compared between formic acid and acetic acid in reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) applications. The results showed that acetic acid resulted in a 2.2-2.5 times increase in MS signal and a slight decrease in retention compared to formic acid. The number of identified peptides was up to 60% higher under acetic acid conditions, depending on the sample type. The retention shift was found to be dependent on peptide net charge and demonstrated the hydrophilicity of acetic acid.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Analytical
P. Montoro-Leal, J. C. Garcia-Mesa, I Morales-Benitez, A. Garcia de Torres, E. Vereda Alonso
Summary: An automatic MSPE method combining MGO-based magnetic nanomaterial with HPLC-ICP-MS for arsenic speciation was developed, achieving automatic preconcentration and separation/determination of arsenic species with low LODs and high precision. The method was validated by analyzing Certified Reference Materials and recovery analysis of arsenic species in various samples, showing potential for routine monitoring of arsenic species in environmental waters and biological fluids.
Article
Biochemical Research Methods
Qian Ma, Qinghe Zhang, Xiao Li, Yan Gao, Chao Wei, Hongmei Li, Hui Jiao
Summary: An enzyme-assisted extraction and ion pairing reversed phase chromatography method coupled to inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) was developed for simultaneous analysis of five selenium species in rice. The study successfully detected different selenium species in eleven rice samples from China and found that SeMet was the dominant selenium form.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Polymer Science
Aya M. Mostafa, Stephen J. Barton, Stephen P. Wren, James Barker
Summary: This study developed a new and highly selective magnetic molecularly imprinted polymers (MIPs) for the extraction of salivary pepsin. The developed MIPs provide a stable, cost-effective, and reusable method for pepsin extraction. The MIPs were successfully used for dispersive solid phase extraction of pepsin and a sensitive HPLC-SEC method was developed for the quantitation of extracted pepsin fragments. The system shows potential as an efficient and reliable testing method for pepsin.
Article
Chemistry, Medicinal
Paulina Hebda, Lilianna Wisniowska, Przemyslaw W. Szafranski, Marek Cegla
Summary: The study presented a method for obtaining optically pure chiral alcohols through lipase-catalyzed hydrolytic kinetic resolution, and investigated parameters of the preparative-scale process. Additionally, an efficient derivatization protocol was developed for chiral compounds enabling their determination by chiral reversed-phase high-performance liquid chromatography (RP-HPLC).
Article
Biochemistry & Molecular Biology
Pawel Zagrodzki, Pawel Pasko, Agnieszka Galanty, Malgorzata Tyszka-Czochara, Renata Wietecha-Posluszny, Pol Salvans Rubio, Henryk Barton, Ewelina Prochownik, Bozena Muszynska, Katarzyna Sulkowska-Ziaja, Katarzyna Bierla, Ryszard Lobinski, Joanna Szpunar, Shela Gorinstein
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2020)
Article
Chemistry, Applied
Ghaya Alchoubassi, Katarzyna Kinska, Katarzyna Bierla, Ryszard Lobinski, Joanna Szpunar
Summary: Coconut water has been shown to contain essential elements such as iron, copper, manganese, and zinc, with concentrations varying depending on the origin and maturity of the coconut. Speciation analysis revealed the presence of various metal species in coconut water, each contributing differently to the total element concentrations.
Article
Biochemistry & Molecular Biology
Marek Kieliszek, Katarzyna Bierla, Javier Jimenez-Lamana, Anna Maria Kot, Jaime Alcantara-Duran, Kamil Piwowarek, Stanislaw Blazejak, Joanna Szpunar
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Plant Sciences
Edith Cruzado-Tafur, Katarzyna Bierla, Lisard Torro, Joanna Szpunar
Summary: The study evaluated the capability of native plant species grown in polluted post-mining soils to accumulate metals for phytoremediation. Different native plants were found suitable for remediation of specific metals, with some being effective for both Cd and Zn. However, none of the studied plants were suitable for phytoremediation of Pb, Cu, As, and Ag.
Article
Biochemistry & Molecular Biology
Marc Dauplais, Katarzyna Bierla, Coralie Maizeray, Roxane Lestini, Ryszard Lobinski, Pierre Plateau, Joanna Szpunar, Myriam Lazard
Summary: The study identified metabolic pathways of MeSeH in Saccharomyces cerevisiae and deciphered its toxicity mechanism, showing that MeSeH is metabolized into selenomethionine which induces toxic protein aggregation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Geosciences, Multidisciplinary
Edith Cruzado-Tafur, Lisard Torro, Katarzyna Bierla, Joanna Szpunar, Esperanca Tauler
Summary: The article evaluates the environmental quality of soils in Mining Environmental Liabilities (MEL) areas in the Peruvian Andes, finding high soil acidity and high concentrations of potentially toxic elements exceeding permissible limits for agricultural soils. It also inventories native flora in the contaminated sites, identifying species that could be used for phytoremediation purposes.
JOURNAL OF SOUTH AMERICAN EARTH SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jordan Sonet, Anne-Laure Bulteau, Zahia Touat-Hamici, Maurine Mosca, Katarzyna Bierla, Sandra Mounicou, Ryszard Lobinski, Laurent Chavatte
Summary: Selenoproteins, which contain the rare amino acid selenocysteine with selenium atom, play crucial roles in cell homeostasis, antioxidant defense, and cell signaling in mammals. The expression of the selenoproteome, comprised of 25 selenoprotein genes, is tightly regulated by the selenium status of the body. The study presents an alternative strategy for characterizing selenoproteome regulation using radioactive Se-75 isotope and non-radioactive selenium isotopes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Pawel Zagrodzki, Pawel Pasko, Enrique Dominguez-Alvarez, Noemi Salardon-Jimenez, Clotilde Sevilla-Hernandez, Carmen Sanmartin, Katarzyna Bierla, Ryszard Lobinski, Joanna Szpunar, Jadwiga Handzlik, Claus Jacob, Urszula Bieniek, Agnieszka Galanty, Shela Gorinstein
Summary: Novel selenorganic compounds were successfully applied to fortify kale sprouts in selenium, resulting in significantly higher selenium content compared to unfortified sprouts. Different selenium compounds delivered varying doses of selenium to the sprouts, with only a small percentage being incorporated as seleno-L-methionine, Se(IV) and Se-methylselenocysteine.
MICROCHEMICAL JOURNAL
(2022)
Article
Agriculture, Multidisciplinary
Hui Zhu, Katarzyna Bierla, Xueli Jin, Joanna Szpunar, Daijie Chen, Ryszard Lobinski
Summary: This study analyzed the Se metabolites of a Se-enriched probiotic and identified gamma-glutamyl-selenomethionine as the major Se metabolite. It also discovered 11 minor Se metabolites, 8 of which were not previously reported in probiotics. These findings demonstrate the potential of this probiotic as an alternative source of organic Se for food and feed supplementation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Cell Biology
Gabrielle Schanne, Martha Zoumpoulaki, Geraldine Gazzah, Amandine Vincent, Hugues Preud'homme, Ryszard Lobinski, Sylvie Demignot, Philippe Seksik, Nicolas Delsuc, Clotilde Policar
Summary: Oxidative stress is important in the pathogenesis of inflammatory bowel diseases (IBDs). Superoxide dismutase (SOD) defenses are weakened in IBD patients. A Mn(II) complex SOD mimic (Mn1) based on an open-chain diaminoethane ligand has antioxidant and anti-inflammatory effects. Metal exchanges occur between the manganese center and metal ions in the biological environment. New Mn(II) complexes with cyclohexyl and/or propyl groups show improved SOD activity and kinetic inertness in metal ion exchange processes. They provide anti-inflammatory and antioxidant effects in cells at lower doses than Mn1.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Chemistry, Analytical
Pawel Pasko, Agnieszka Galanty, Pawel Zagrodzki, Pawel Zmudzki, Urszula Bieniek, Ewelina Prochownik, Enrique Dominguez-Alvarez, Katarzyna Bierla, Ryszard Lobinski, Joanna Szpunar, Jadwiga Handzlik, Monika Marcinkowska, Shela Gorinstein
Summary: This study investigates the effect of novel selenium organic compounds on the biological activity of kale sprouts. The results show that fortification of kale sprouts with these compounds enhances antioxidant properties and exhibits chemoprotective effects in various cancer types.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Pawel Zagrodzki, Agnieszka Wiesner, Monika Marcinkowska, Marek Jamrozik, Enrique Dominguez-Alvarez, Katarzyna Bierla, Ryszard Lobinski, Joanna Szpunar, Jadwiga Handzlik, Agnieszka Galanty, Shela Gorinstein, Pawel Pasko
Summary: Due to selenium deficiency in humans, the search for new organic molecules containing this element in plant biofortification is important. This study evaluated selenium organic esters based on benzoselenoate scaffolds, with additional halogen atoms and various functional groups. The study aimed to discover the relationships between molecular characteristics of these compounds and the amount of sulfur phytochemicals in kale sprouts. The statistical model revealed a correlation structure between the molecular descriptors of selenium compounds and the biochemical features of the sprouts.
Article
Biochemistry & Molecular Biology
Hui Zhu, Katarzyna Bierla, Jun Tan, Joanna Szpunar, Daijie Chen, Ryszard Lobinski
Summary: This study investigated the influence of the fermentation process on selenite metabolism and selenium enrichment in the probiotic Bifidobacterium longum DD98. The study evaluated the effects of sodium selenite concentration, feeding time, and fermentation stage on the distribution of selenium compounds and the speciation of selenium in the metabolite fraction. The results showed that the fermentation process significantly affected the incorporation of selenium into metabolites and proteins in B. longum DD98, offering a tool for optimizing the production of selenium-enriched probiotics with desired molecular speciation.
Article
Chemistry, Analytical
Katarzyna Kinska, Ghaya Alchoubassi, Luluil Maknun, Katarzyna Bierla, Ryszard Lobinski, Joanna Szpunar
Summary: A quantification method based on the in situ formation of isotopically-labelled iron species as internal standards was developed for the analysis of iron species in plant fluids. The method does not require prior knowledge of the molecular structures of the iron species, eliminating the need for molecular standards, and can effectively correct for on-column phenomena that may affect quantitative analyses.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2022)
Article
Chemistry, Analytical
J. Lamarche, K. Bierla, L. Ouerdane, J. Szpunar, L. Ronga, R. Lobinski
Summary: The reactivity of selenoprotein P with two metallodrugs (auranofin and cisplatin) was investigated through a series of purification techniques and sequencing, showing specific interactions between the protein and the drugs.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Michelle Buoso, Beatrice Campanella, Massimo Onor, Emanuela Pitzalis, Alessandro D'Ulivo
Summary: The use of BH intermediates as derivatization reagents significantly improves the tolerance to interference from transition metal ions in the generation of SbH3 and BiH3, and shows high tolerance in a wide range of acidity. The method has been successfully applied to the determination of Sb and Bi in complex samples, providing further understanding of the chemical behavior of borohydrides.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2024)
Article
Chemistry, Analytical
Chao-Feng Li, Xuan-Ce Wang, Zhu-Yin Chu, Peng Peng
Summary: Thermal ionization mass spectrometry (TIMS) is the standard method for obtaining precise Sr-87/Sr-86 isotopic ratios. However, our developed hydrofluoric acid coprecipitation method offers a low-cost, rapid, simple, and high-throughput alternative for analyzing red wine samples, effectively eliminating isobaric interference and matrix element inhibition.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2024)
Article
Chemistry, Analytical
Ivan B. Krstic, Bratislav M. Obradovic, Milorad M. Kuraica
Summary: This paper presents the spatio-temporal dynamics of a Grimm-type microsecond pulsed glow discharge (mu PGD) in argon at 6 mbar. The occurrence of a high current peak during the discharge ignition is analyzed and the importance of displacement current is emphasized. The development of electron excitation temperature for argon atoms and ions at various distances from the cathode is measured. The spatio-temporal evolution of selected spectral lines of argon and copper atoms and ions is analyzed during different phases of the discharge. The results suggest that the behavior of spectral line emissions differs between the pre-peak and afterglow periods, with different lines reaching maximum intensity during these phases. The appearance time and decay time of the afterpeak are shorter for ions than for atoms. These findings are explained in terms of three-body recombination processes and ion mobilities.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2024)
Article
Chemistry, Analytical
Qiuyun Wang, Anmin Chen, Xun Gao
Summary: In this paper, the combination of Tesla coil discharge (TCD) and laser-induced breakdown spectroscopy (LIBS) was used to enhance the emission of Al atomic lines and AlO molecular bands. The optimized distance between the TCD tip and the sample surface was found to be 5 mm, and the optimal laser energy was 25.1 mJ. Furthermore, TCD-SELIBS showed lower limits of detection (LoDs) for Cr and Pb elements compared to surface enhanced LIBS (SELIBS). The combination of TCD and LIBS is a promising technology for high-sensitivity analysis of heavy metals in water, with the advantages of simplicity, low cost, ease of operation, industrialization, and commercialization.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2024)
Article
Chemistry, Analytical
Yanwen Tang, Tingguang Lan, Jianfeng Gao, Zhongjie Bai, Xiaowen Huang, Junjie Han, Na Liu
Summary: Titanium is an important metal widely used in various industries. Ilmenite minerals are the main source of titanium metal and can be found in igneous and metamorphic rocks as well as different types of deposits. To accurately determine U-Pb ages of ilmenite, a new calibration method and appropriate reference materials are urgently needed. Laser ablation sector field inductively coupled plasma mass spectrometry has been used to minimize matrix effects and zircon 91500 has been identified as a suitable reference material for measuring the Pb/U ratio in ilmenite. The direct calibration method using zircon 91500 as an external standard has been found to be effective for in situ U-Pb dating of ilmenite samples under different ablation settings.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2024)
