Article
Cell Biology
Gea Cereghetti, Caroline Wilson-Zbinden, Vera M. Kissling, Maren Diether, Alexandra Arm, Haneul Yoo, Ilaria Piazza, Shady Saad, Paola Picotti, D. Allan Drummond, Uwe Sauer, Reinhard Dechant, Matthias Peter
Summary: The glycolytic metabolite fructose-1,6-bisphosphate is able to initiate the disassembly of inactive amyloids formed by Cdc19 during stress, leading to the restoration of ATP production and disassembly of stress granules (SGs) in yeast cells. This mechanism directly links cellular metabolism with SG dynamics, and plays a critical role in stress recovery by promoting Cdc19 re-solubilization and enhancing ATP production.
NATURE CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Benya Nontaleerak, Chatchakorn Eurtivong, Churat Weeraphan, Hansuk Buncherd, Daranee Chokchaichamnankit, Chantragan Srisomsap, Jisnuson Svasti, Rojana Sukchawalit, Skorn Mongkolsuk
Summary: This study found that oxidation of C104 in the NieR protein under NaOCl stress leads to the formation of a disulfide-linked dimer, altering the protein's conformation and thereby abolishing its DNA-binding ability and repressing the expression of target genes.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Danielle M. Beaupre, Richard G. Weiss
Summary: The review focuses on the properties and applications of synthetic thiol- and disulfide-based materials, particularly polymers. The emphasis is on soft and self-assembling materials, where interconversion of thiol and disulfide groups can trigger stimulus-responses and self-healing, for both biomedical and non-biomedical applications.
Article
Electrochemistry
Daria A. Burmistrova, Andrey Galustyan, Ivan V. Smolyaninov, Nadezhda T. Berberova
Summary: A range of substituted o-aminophenols have been investigated as redox mediators for thiol oxidation to disulfides. The electrooxidation of o-aminophenols leads to the formation of o-iminobenzoquinones, which react with thiols in solution to form disulfides. The use of 4,6-di-tert-butyl-2-(tert-butylamino)phenol as a redox mediator was found to reduce the overpotential of thiol oxidation, resulting in unsymmetrical disulfides with alkyl, aryl, and heteroaryl substituents.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Natalia Zamorano Cuervo, Nathalie Grandvaux
Summary: Protein disulfide isomerases (PDIs) are enzymes that catalyze redox reactions and have chaperone functions in protein folding. PDIs have catalytic thioredoxin (TRX)-like domains with CXXC motifs for redox reactions, as well as non-catalytic TRX-like domains. Strategies for identifying cysteine oxidative post-translational modifications (Cys ox-PTMs) have been optimized, and analysis of a Cys redoxome dataset suggests the presence of a non-catalytic thiol-disulfide motif in PDIs. Further structural analysis and exploration of other PDIs are needed to understand the role of these motifs.
Article
Biochemistry & Molecular Biology
Eshita Das, Shivcharan Prasad, Ipsita Roy
Summary: Peptidyl prolyl isomerases play a key role in accelerating protein folding, with larger multi-domain proteins having additional functions beyond isomerization. Yeast Fpr1 exhibits characteristics of a general chaperone in the proteostasis network, reducing protein aggregation and improving cell survival. Overexpression of Fpr1 protects cells against thermal shock, suggesting potential therapeutic implications in disease conditions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Rebecca L. Charles, Giancarlo Abis, Beatriz F. Fernandez, Sebastian Guttzeit, Roberto Buccafusca, Maria R. Conte, Philip Eaton
Summary: The study revealed that H2O2 activates sEH by forming an intra-disulfide bond, increasing its catalytic efficiency. This oxidative activation mechanism also sensitizes sEH to inhibition by lipid electrophiles. The renin-angiotensin system regulates the intra-disulfide bond activation of sEH, leading to a decrease in EET/DHET ratio and an increase in blood pressure.
Article
Biochemistry & Molecular Biology
Tianqi Wang, Yuqing Yang, Menghui Liu, Honglei Liu, Huaiwei Liu, Yongzhen Xia, Luying Xun
Summary: Elemental sulfur serves as a strong oxidant, entering yeast cells and reacting with glutathione to produce glutathione persulfide, H2S, and glutathione disulfide, leading to inhibited oxygen consumption and increased reactive oxygen species, ultimately inhibiting yeast growth.
Review
Chemistry, Analytical
Zilong Song, Chengwu Fan, Jintao Zhao, Lei Wang, Dongzhu Duan, Tong Shen, Xinming Li
Summary: The modulation of numerous signaling pathways is controlled by redox regulation. Maintaining dynamic redox homeostasis is crucial for human health due to the common occurrence of altered redox status in various pathological conditions. Mammalian thioredoxin reductase (TrxR), a vital component of the thioredoxin system, plays a crucial role in supporting various physiological functions, but its dysfunction disrupts redox balance and is closely associated with the pathogenesis of multiple diseases.
Article
Chemistry, Multidisciplinary
Jingwen Xu, Xiangji Yan, Yue Zhang, Kangsheng Tu, Wen Shen, Zhaoqing Tian, Guoliang Li, Wei Zhao, Mingzhen Zhang
Summary: A study was conducted to achieve tunable release of paclitaxel (PTX) by fabricating thermal and redox-responsive copolymer nanomicellar assemblies. The release behavior of the nanomicelles was found to be dependent on their structure and assembly ratio, which was validated through cellular uptake and in vivo anti-tumor experiments.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Article
Chemistry, Physical
Xun Zhang, Bin Yang, Wenlong Zhou, Zhenqi Zhu, Yujing Bian, Ruonan Zeng
Summary: This study utilized FT-Raman and THz-TDS spectroscopy techniques to measure the TRS, showing a good correlation between disulfide concentration and characteristic peak intensities, with correlation coefficients of 92.2% and 95.4% for FT-Raman and THz-TDS data, respectively. Solid-state density functional theory analysis revealed that both Raman and terahertz vibrational spectroscopy techniques can quantitatively analyze the ratio of cysteine/cystine, providing a new method for biological analysis of sulfur-containing amino acids and related mixtures in basic science.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Biophysics
Xia Liu, Lei Qin, Jie Yu, Wentao Sun, Jianhong Xu, Chun Li
Summary: During industrial fermentation, microbial cell factories often face environmental or metabolic stresses, resulting in imbalanced intracellular redox and decreased metabolic capacity. In this study, a genetically encoded redox biosensor system (RBS) based on redox-sensitive fluorescent proteins was constructed for detecting redox metabolites in Saccharomyces cerevisiae. The RBS allowed for the exploration of compartmental redox state diversity and real-time monitoring of the yeast production process, providing a reliable and effective approach for profiling bottlenecks in yeast cell factories.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Pharmacology & Pharmacy
Sonyabapu Yadav, Kalyan Ramesh, Obireddy Sreekanth Reddy, Viswanathan Karthika, Parveen Kumar, Sung-Han Jo, Seong Yoo, Sang-Hyug Park, Kwon Taek Lim
Summary: In this study, redox-responsive core-cross-linked (CCL) micelles were synthesized using diselenide (Se-Se) and disulfide (S-S) bonds, and their sensitivity to oxidation and reduction was compared. The micelles showed structural stability under physiological conditions but de-cross-linked in response to GSH and H2O2 treatments. DLS studies demonstrated that the Se-Se micelles were more responsive to changes in the redox environment compared to the S-S micelles. The developed micelles exhibited a pH-dependent drug release and showed cytotoxicity against cancer cells while remaining non-toxic to normal cells.
Article
Biotechnology & Applied Microbiology
Javier Garcia-Pardo, Aleksandra E. Badaczewska-Dawid, Carlos Pintado-Grima, Valentin Iglesias, Aleksander Kuriata, Sebastian Kmiecik, Salvador Ventura
Summary: This study presents the A3D yeast database (A3DyDB), which provides an extensive catalog of aggregation propensity predictions for the S. cerevisiae proteome, allowing for the evaluation of the influence of mutations on protein stability and solubility.
MICROBIAL CELL FACTORIES
(2023)
Article
Medicine, Research & Experimental
Jie Lei, Qian Zhang, Xuan Jin, Huiru Lu, Shuxiang Wang, Tingting Li, Yanmei Sheng, Fangyan Zhang, Yaxin Zheng
Summary: The drug release mechanism and rate of camptothecin-oleic acid prodrugs linked by disulfide bonds were found to be highly dependent on the reactivity between the disulfide bonds and thiol agents, affecting the release rate of the drug. The extracellular release of CPT was significantly faster than intracellular release, attributed to different release mechanisms.
MOLECULAR PHARMACEUTICS
(2021)
Review
Chemistry, Inorganic & Nuclear
Mikel Bernabeu de Maria, Jeremy Lamarche, Luisa Ronga, Luigi Messori, Joanna Szpunar, Ryszard Lobinski
Summary: This review critically discusses the actual location of Se-metal bonds and the involvement of selenol in proteins containing multiple SeCys residues. It also examines the applications of advanced mass spectrometric and atomic spectroscopic techniques in studying metal-binding sites.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Environmental Sciences
Sebastiano Gozzo, Samuel Moles, Katarzyna Kinska, Maria P. Ormad, Rosa Mosteo, Jairo Gomez, Francisco Laborda, Joanna Szpunar
Summary: A UPLC-MS/MS method was optimized for the analysis of antibiotics and their metabolites in surface and wastewaters. The method was applied to monitor the river basin of the POCTEFA territory, and 21 antibiotics and their metabolites were detected. Fluoroquinolones and sulfamethoxazole, as well as their metabolites, should be considered as target compounds in the monitoring of water resources in the POCTEFA territory.
Article
Chemistry, Analytical
Luluil Maknun, Katarzyna Kinska, Ivain Gonzalez-Alvarez, Laurent Ouerdane, Beiatrice Lauga, Atitaya Siripinyanond, Joanna Szpunar, Ryszard Lobinski
Summary: A method for quantifying iron-siderophore complexes by electrospray high-resolution accurate mass (HRAM) mass spectrometry without the use of authentic standards was developed. The complexes were purified by solid-phase extraction (SPE) and identified by fast size-exclusion chromatography (FastSEC)-Orbitrap MS(n) based on molecular mass and fragmentation. The ability of the complexes to exchange Fe-56 and Fe-58 was demonstrated by SEC with ICP MS and ESI MS detection. The method was applied to analyze peat samples from the French Pyrenean mountains and successfully identified and quantified 19 siderophores belonging to different classes.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
D. Kalampaliki, G. D. T. M. Jayasinghe, E. Avramiotis, I. D. Manariotis, D. Venieri, S. G. Poulopoulos, J. Szpunar, J. Vakros, D. Mantzavinos
Summary: In this study, valorized biomass was pyrolyzed and activated with potassium hydroxide to produce a biochar with a high specific surface area and significant amounts of surface oxygen species and carbonates. The activated biochar was used as an activator for sodium persulfate to induce the adsorption and oxidation of sulfamethoxazole in various water matrices. The degradation of sulfamethoxazole followed a pseudofirst order kinetic law, and the oxidation mechanism involved radical pathways and/or electron transfer.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2023)
Article
Chemistry, Inorganic & Nuclear
Luisa Ronga, Iogann Tolbatov, Ester Giorgi, Paulina Pisarek, Christine Enjalbal, Alessandro Marrone, Diego Tesauro, Ryszard Lobinski, Tiziano Marzo, Damiano Cirri, Alessandro Pratesi
Summary: A panel of four gold(I) complexes, inspired by auranofin, were reported by the research group. These complexes showed similarities to auranofin but exhibited distinct features, such as lower log P values. Experimental and theoretical studies revealed that structural modifications on the phosphine ligand of auranofin can impact the chemical properties and biological mode of action of the derivatives.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Pawel Zagrodzki, Agnieszka Wiesner, Monika Marcinkowska, Marek Jamrozik, Enrique Dominguez-Alvarez, Katarzyna Bierla, Ryszard Lobinski, Joanna Szpunar, Jadwiga Handzlik, Agnieszka Galanty, Shela Gorinstein, Pawel Pasko
Summary: Due to selenium deficiency in humans, the search for new organic molecules containing this element in plant biofortification is important. This study evaluated selenium organic esters based on benzoselenoate scaffolds, with additional halogen atoms and various functional groups. The study aimed to discover the relationships between molecular characteristics of these compounds and the amount of sulfur phytochemicals in kale sprouts. The statistical model revealed a correlation structure between the molecular descriptors of selenium compounds and the biochemical features of the sprouts.
Article
Fisheries
G. D. T. M. Jayasinghe, Joanna Szpunar, Ryszard Lobinski, E. M. R. K. B. Edirisinghe
Summary: A UPLC-MS/MS method was developed and validated for the quantitative confirmatory analysis of multi-class antibiotics residues in fish and shrimp. The method successfully detected 14 antibiotics belonging to different classes. The concentrations of antibiotics found in the samples complied with the EU regulation limits for veterinary drug residues in seafood and did not pose a risk in terms of food safety.
Article
Engineering, Environmental
Francisco Calderon Celis, Ivan Gonzalez-Alvarez, Magdalena Fabjanowicz, Simon Godin, Laurent Ouerdane, Beatrice Lauga, Ryszard Lobinski
Summary: Metallophores play a crucial role in metal scavenging and acquisition for many organisms. However, their identification in highly organic matter rich environments like peatlands is challenging due to low solubilization and metal scarcity. In this study, a novel two-dimensional solid-phase extraction-liquid chromatography-mass spectrometry (SPE-LC-MS) approach was developed for sensitive detection and identification of metallophores in native environments. By applying this method, 53 metallophores, including 36 bacterial ones, were identified in peat collected from a French peatland. The detection of characteristic metal isotope patterns also revealed potential complexes of iron and copper. Furthermore, a taxonomic-based inference method was implemented to associate the identified bacterial metallophores with potential producers, suggesting a new way to explore poorly documented environments for new metallophores and their producers.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Mikel Bernabeu de Maria, Diego Tesauro, Filippo Prencipe, Michele Saviano, Luigi Messori, Christine Enjalbal, Ryszard Lobinski, Luisa Ronga
Summary: Methylmercury, mercury (II), and mercury (I) chlorides were found to react with vasopressin and its mono- and diselenium analogues to form various mercury-peptide adducts. The replacement of Cys by SeCys in vasopressin increased reactivity towards methylmercury, resulting in the formation of -Se/S-Hg-Se-bridged structures and subsequent demethylation of methylmercury. Competitive experiments showed that CH3HgCl preferred to react with the diselenium analogue rather than vasopressin. The diselenium peptide also demonstrated the ability to displace CH3Hg moieties bound to S in vasopressin. These findings suggest the potential use of selenopeptides for methylmercury chelation and detoxification strategies.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Bierla, Simon Godin, Marta Ladanyi, Mihaly Dernovics, Joanna Szpunar
Summary: Automated and specific picking of selenium-containing molecular entities is achieved through a comprehensive pattern matching approach, and the reproducibility and selenium isotope selection are critically evaluated. Hydrophilic interaction liquid chromatography is used for one-step separation of water soluble metabolites, and absolute mass defect (MD) data are assessed by multivariate statistical tools for selenometabolite-specific screening.
Article
Biochemistry & Molecular Biology
Hui Zhu, Katarzyna Bierla, Jun Tan, Joanna Szpunar, Daijie Chen, Ryszard Lobinski
Summary: This study investigated the influence of the fermentation process on selenite metabolism and selenium enrichment in the probiotic Bifidobacterium longum DD98. The study evaluated the effects of sodium selenite concentration, feeding time, and fermentation stage on the distribution of selenium compounds and the speciation of selenium in the metabolite fraction. The results showed that the fermentation process significantly affected the incorporation of selenium into metabolites and proteins in B. longum DD98, offering a tool for optimizing the production of selenium-enriched probiotics with desired molecular speciation.
Article
Biochemistry & Molecular Biology
Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski, Roger A. Sunde
Summary: This study found that the metabolism and accumulation of selenomethionine in rats is different from that of selenocysteine. At the selenium requirement for maximal glutathione peroxidase activity, selenocysteine is the major selenium species, while at higher intakes of selenomethionine, it is metabolized into low molecular weight selenosugars and selenosugar-decorated proteins. Additionally, compared to selenite, selenomethionine leads to less binding of inorganic selenium to liver proteins.
Article
Chemistry, Analytical
Adam Sajnog, Katarzyna Bierla, Joanna Szpunar, Javier Jimenez-Lamana
Summary: This article discusses reliable quantification methods and sample preparation protocols for biogenic selenium nanoparticles. Ten different extraction approaches were compared and their efficiency was evaluated using single particle - ICP-MS. The results showed that enzymatic digestion resulted in the highest recoveries but also the broadest size distribution.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Chemistry, Multidisciplinary
Sergio Fernandez-Castillo Suarez, Cecile Courreges, Javier Jimenez-Lamana, Simon Godin, Sophie Nolivos, Regis Grimaud, Luisa Ronga, Joanna Szpunar, Joachim Allouche
Summary: The study of interaction mechanisms between nanomaterials and microbial cells at the nanoscale is crucial in various research fields. This paper demonstrates the biodegradation process of gelatin adsorbed on the surface of nanoparticles by Alteromonas macleodii extracellular proteases.
ENVIRONMENTAL SCIENCE-NANO
(2023)