4.7 Article

Reversed Phase HPLC Analysis of Stability and Microstructural Effects on Degradation Kinetics of β-Carotene Encapsulated in Freeze-Dried Maltodextrin-Emulsion Systems

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 60, 期 38, 页码 9711-9718

出版社

AMER CHEMICAL SOC
DOI: 10.1021/jf303452c

关键词

freeze-drying; emulsion; beta-carotene; structure; particle size

资金

  1. Thailand Research Fund through the Royal Golden Jubilee Ph.D. program [PHD/0224/2549]
  2. Food Institutional Research Measure (FIRM) [08-RDC-695]
  3. Department of Agriculture, Food and Marine (DAFM) Ireland

向作者/读者索取更多资源

Degradation of dispersed lipophilic compounds in hydrophilic solids depends upon matrix stability and lipid physicochemical properties. This study investigated effects of solid microstructure and size of lipid droplets on the stability of dispersed beta-carotene in freeze-dried systems. Emulsions of beta-carotene in sunflower oil were dispersed in maltodextrin systems (M040/DE6, M100/DE11, and M250/DE25.5) (8% w/w oil) and prefrozen at various freezing conditions prior to freeze-drying to control nucleation and subsequent pore size and structural collapse of freeze-dried solids. The particle size, physical state, and beta-carotene contents of freeze-dried emulsions were measured during storage at various water activity (a(w)) using a laser particle size analyzer, differential scanning calorimeter, and high performance liquid chromatography (HPLC), respectively. The results showed that M040 stabilized emulsions in low temperature freezing exhibited lipid crystallization. Collapse of solids in storage at a(w) which plasticized systems to the rubbery state led to flow and increased the size of oil droplets. Degradation of beta-carotene analyzed using a reversed-phase C-30 column followed first-order kinetics. Porosity of solids had a major effect on beta-carotene stability; however, the highest stability was found in fully plasticized and collapsed solids.

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