4.7 Article

Differences in Pig Muscle Proteome According to HAL Genotype: Implications for Meat Quality Defects

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 57, 期 11, 页码 4913-4923

出版社

AMER CHEMICAL SOC
DOI: 10.1021/jf900286x

关键词

2-DE gels; pig muscle; hierarchical clustering analysis; malignant hyperthermia; meat quality; energetic metabolism; heat shock proteins; antioxidant proteins

资金

  1. Agence Nationale de la Recherche, Programme Alimentation-Nutrition Humaine [ANR-06PNRA-025-05]

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Bidimensional electrophoresis was used to compare sarcoplasmic protein profiles of early postmortem pig semimembranosus muscles, sampled from pigs of different HAL genotypes (RYR1 mutation 1841T/C): 6 NN, 6 Nn, 6 nn. ANOVA showed that 55 (18%) of the total of 300 matched protein spots were influenced by genotype, and hierarchical clustering analysis identified 31 (10% of the matched proteins) additional proteins coregulated with these proteins. Fold-changes of differentially expressed proteins were between 1.3 and 21.8. Peptide mass fingerprinting identification of 78 of these 86 proteins indicates that faster pH decline of nn pigs was not explained by higher abundance of glycolytic enzymes. Results indicate further that nn muscles contained fewer proteins of the oxidative metabolic pathway, fewer antioxidants, and more protein fragments. Lower abundance of small heat shock proteins and myofibrillar proteins in nn muscles may at least partly be explained by the effect of pH on their extractability. Possible consequences of lower levels of antioxidants and repair capacities, increased protein fragmentation, and lower extractability of certain proteins in nn muscles on meat quality are discussed.

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