Article
Entomology
Aristotelis Moulistanos, Nikoleta Karaiskou, Konstantinos Gkagkavouzis, Styliani Minoudi, Elena Drosopoulou, Chrysanthi Ioannidou, Nikolas Panteli, Stella Zografou, Damianos Karaouglanis, Dimitrios Kotouzas, Dimitrios Kontodimas, Efthimia Antonopoulou, Alexandros Triantafyllidis
Summary: This study used a genetic method to detect the composition of insect meals, successfully identified insect individuals, and analyzed the composition of nine insect meals. Errors in labeling commercially available larvae were detected during this process. No other insect species or animal species than reported were found in commercially available insect meals, while fungal species were identified in non-processed insect meal. Through this study, the use of DNA has been validated as a tool that increases the safety and quality of insect products.
Article
Entomology
Jian-Zhao Jiang, Bo-Yu Huang, Qian Wu, Shi-Yu Li, Jun Gu, Li-Hua Huang
Summary: A rapid identification method based on PCR-RFLP was developed to distinguish the two strains of FAW and other lepidopteran pests. The unique digestion patterns of four species, including FAW, were revealed by sequencing their mitochondrial COI genes. Furthermore, a specific SNP site was found in the corn strain FAW, allowing its distinction from the rice strain. Testing 28 FAW samples collected from different locations and host plants in China confirmed the presence of the corn strain only, indicating that the rice strain has not invaded China yet. This method enables differentiation of FAW from other lepidopteran pests and identification of the two FAW host strains.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Environmental Sciences
Lise Helen Ofstad, Hjalmar Hatun, Torstein Pedersen, Petur Steingrund, Bjarni Mikkelsen
Summary: This study investigated the migration and distribution patterns of adult anglerfish in the Faroese waters. It was found that the fish displayed a clear seasonal offshore-onshore migration, occupying shallower waters during summer and deeper waters, particularly on the western side of the Faroe shelf, during winter. The fish showed vertical activity variations, with higher activity during winter and night time.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Food Science & Technology
Luca Maria Pennisi, Ambra Rita Di Rosa, Luca Nalbone, Filippo Giarratana
Summary: The present study updated the previously proposed quality index (QI) scheme for fresh and whole Lophius piscatorius stored in ice by including 3 characters, namely appearance, eye, and fin, for a total of 18 demerit points. A positive linear correlation between QI score and storage time was observed, and the proposed scheme offers a ready-to-use freshness assessment of the anglerfish.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Infectious Diseases
Muslimah N. Alsulami, Majed H. Wakid, Mohammed Al-matary, Rewaida Abdel-Gaber, Wafa Abdullah Al-Megrin, Areej O. Bakhraibah, Abdullah D. Alanazi, Hatem A. Elshabrawy, Asmaa M. El-Kady
Summary: This study investigated the genetic diversity of Fasciola spp. isolated from cattle in Saudi Arabia using sequence analysis of the COI gene. The results showed that F. hepatica was the only Fasciola species parasitizing cattle in Jeddah city, Saudi Arabia. Further studies with more samples from different regions in Saudi Arabia are needed to develop control measures against fascioliasis.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Agriculture, Multidisciplinary
A. Khalid, M. Imran, A. Ali, S. Muzammil, M. Badar, S. Hayat, I. R. Malik
Summary: This study demonstrates the use of molecular techniques, specifically PCR-RFLP, to identify different meat animal species. The results show that by utilizing the mitochondrial COI gene and TasI and TruI restriction enzymes, reliable species-specific profiles can be generated to distinguish and identify each species. The study suggests that PCR-RFLP is a quick and reliable method that can be widely employed in food control laboratories.
JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS
(2022)
Article
Agriculture, Dairy & Animal Science
V. Vigolo, M. Franzoi, F. Cendron, G. Salvadore, M. Penasa, M. Cassandro, M. De Marchi
Summary: This study compared different methods and technical engagement for identifying the A1 and A2 variants of 0-casein in milk. The findings showed that automated DNA extraction and chromatoallele-specific PCR techniques are fast and reliable options.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Plant Sciences
Shu Zhu, Qundong Liu, Jingyu He, Naomi Nakajima, S. P. Samarakoon, Swe Swe, Khin Zaw, Katsuko Komatsu
Summary: Salacia species have been traditionally used in Ayurvedic medicine for various purposes. This study aims to authenticate these plants and determine the sources of commercially available Salacia-derived products using genetic approaches. Results showed that S. reticulata and S. chinensis are major sources of these products, and a PCR-RFLP assay was established for their identification.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Bushra A. Kanaan, Mushtak T. S. Al-Ouqaili, Rafal M. Murshid
Summary: This study aimed to establish the frequency and pattern of chromosomal abnormalities in women with primary and secondary amenorrhea and detect genetic changes in specific genotypes. The results highlighted the importance of cytogenetic analysis for determining the genetic basis of amenorrhea and suggested genotyping for women with normal karyotype and high FSH serum levels for accurate diagnosis and appropriate management.
Article
Microbiology
Mercedes Fernandez-Escobar, Federica Giorda, Virgina Mattioda, Tania Audino, Fabio Di Nocera, Giuseppe Lucifora, Katia Varello, Carla Grattarola, Luis Miguel Ortega-Mora, Cristina Casalone, Rafael Calero-Bernal
Summary: This study investigated the genetic diversity of Toxoplasma gondii strains in stranded dolphins found in the Mediterranean coastlines of Italy. The results showed that dolphins were mainly infected with ToxoDB#3 and #2 genotypes.
Article
Veterinary Sciences
Gaetano Cammilleri, Stefano D'Amelio, Vincenzo Ferrantelli, Antonella Costa, Maria Drussilla Buscemi, Annamaria Castello, Emanuela Bacchi, Elisa Goffredo, Maria Emanuela Mancini, Serena Cavallero
Summary: Four dead Phalacrocorax carbo sinensis specimens from Southern Italy coasts were examined for the presence of Contracaecum sp. A total of 181 Contracaecum specimens, including larvae and adults, were found in the examined specimens. Contracaecum rudolphii A and B were detected, with a co-infestation of both in one of the specimens from Leporano Bay. This study provides the first report of Contracaecum sp. presence in P. carbo sinensis from Southern Italy.
VETERINARY SCIENCES
(2023)
Article
Marine & Freshwater Biology
Deirdre Brophy, Silvia Perez-Mayol, Roxanne Duncan, Karin Hussy, Audrey J. Geffen, Hans D. Gerritsen, Maria Ching Villanueva, Beatriz Morales-Nin
Summary: This study investigated the seasonal variation in trace element composition of white anglerfish otoliths and illicia and found that elemental concentrations at the edge of the illicium were primarily related to fish length, while Sr concentrations in otoliths were highest in anglerfish collected during wintertime. Although the seasonal differences in Sr were statistically significant but small, the seasonal pattern was consistently detected across all size classes.
ESTUARINE COASTAL AND SHELF SCIENCE
(2021)
Article
Fisheries
Haitao Ma, Hongmei Gao, Yang Zhang, Yanping Qin, Zhiming Xiang, Jun Li, Yuehuan Zhang, Ziniu Yu
Summary: This study demonstrates the utility of a multiplex species-specific PCR for identifying five giant clam species in the South China Sea based on mitochondrial COI gene markers. The approach allows for rapid, reliable, and cost-effective identification, improving the efficiency and quality of giant clam stock management, protection, and forensic identification by reducing the risk of misidentification.
Article
Biotechnology & Applied Microbiology
Ibai Nafarrate, Estibaliz Mateo, Katherine Miranda-Cadena, Amaia Lasagabaster
Summary: This study reported the isolation and characterization of 304 Campylobacter specific bacteriophage isolates from broiler and swine sources. The most promising 59 campylophage candidates were selected based on their lytic activity, and RAPD-PCR technique was found to be effective only for Group II campylophages. Bacteriophage isolates within the same genotype displayed different host ranges, leading to the differentiation of 13 campylophages from Group II and 8 from Group III.
Article
Biodiversity Conservation
Wanlada Klangnurak, Sirachai Arunrugstichai, Petch Manopawitr, Tassapon Krajangdara
Summary: This study examined the species composition and conservation statuses of shark fin products in Thailand using DNA-based species identification. At least 15 shark species were found in Thailand's markets, with the spottail shark and the night shark being the dominant species. There were 34% of identifiable samples that were previously unrecorded in the region, and 62% of species detected from the fins were classified as threatened on the IUCN Red List. The species composition of shark fin products can help guide conservation actions and raise awareness about the trade in elasmobranch products.
CONSERVATION GENETICS
(2023)