Article
Chemistry, Applied
Tao Song, Xiaotao Wang, Minghao Wu, Kelei Zhao, Xinrong Wang, Yiwen Chu, Jiafu Lin
Summary: In this study, predicted agar polysaccharide utilization loci were used to identify six agarases, with two of them successfully expressed and analyzed. An enzyme cocktail showed the highest synergistic effect for NAOs production. Homogenization was proven to be an efficient way to release agar, resulting in maximum NAOs production of 90.2 mg per 9 g wet G. amansii. The proposed enzyme supported one-step process (ESOP) is potentially an environmentally friendly, time-saving, cost-efficient, and non-destructive method for industrial application in red algae utilization.
Article
Microbiology
Cheng Xue, Zhang-Xian Xie, Yuan-Yuan Li, Xiao-Huang Chen, Geng Sun, Lin Lin, Stephen J. Giovannoni, Da-Zhi Wang
Summary: The study focused on the growth and gene expression of the cultivated SAR92 strain HTCC2207 under different polysaccharide conditions. Results showed that xylan and laminarin supported the growth better, with different sugar substrates inducing specific GH genes expressions. The research provides insight into the complex polysaccharide utilization by HTCC2207 and reconstructs metabolic pathways based on PULs genes and other carbohydrate-active enzymes.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Microbiology
Xiaoyi Wang, Ziwei Wei, Hao Wu, Yujiao Li, Feng Han, Wengong Yu
Summary: This study identified a marine bacterium V. alginolyticus LWW-9 capable of degrading hyaluronic acid, and revealed that it harbors a gene cluster involved in the degradation of hyaluronic acid. Two novel PL8 family hyaluronate lyases were identified as key enzymes for this degradation. A model for the complete degradation of hyaluronic acid by V. alginolyticus LWW-9 was proposed based on genomic and functional analysis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Jiajing Li, Xiaojie Pei, Changhu Xue, Yaoguang Chang, Jingjing Shen, Yuying Zhang
Summary: A new alginate polysaccharide utilization locus (PUL) was discovered, containing four novel alginate lyases. These lyases demonstrated synergistic action and could completely degrade alginate to monosaccharides. These findings are of great significance for alginate degradation and biological fermentation.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Environmental Sciences
Edson Silva, Francois Counillon, Julien Brajard, Anton Korosov, Lasse H. Pettersson, Annette Samuelsen, Noel Keenlyside
Summary: Phytoplankton blooms play a crucial role in marine ecosystems by providing biomass, aiding carbon sequestration, and potentially posing risks when harmful species are involved. The seasonal blooms in the Barents, Norwegian, and North seas are influenced by factors such as mixed layer depth, sea surface temperature, wind speed, and suspended particulate matter. Variability in the timing and intensity of spring and summer blooms in these regions can be attributed to unique environmental conditions and oceanographic processes.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Limnology
Heather M. McNair, Francoise Morison, Jason R. Graff, Tatiana A. Rynearson, Susanne Menden-Deuer
Summary: Microzooplankton grazing has a significant impact on phytoplankton growth and abundance, leading to a decrease in phytoplankton quantity balanced by an increase in phytoplankton cell size. The grazing rate of microzooplankton greatly affects the growth rate of phytoplankton, aligning closely with the observed dynamics of phytoplankton biomass.
LIMNOLOGY AND OCEANOGRAPHY
(2021)
Article
Ecology
Ricardo Gonzalez-Gil, Neil S. Banas, Eileen Bresnan, Michael R. Heath
Summary: The study in a coastal region of the Scottish North Sea supports the validity of the Disturbance Recovery Hypothesis (DRH) for explaining the onset of phytoplankton biomass accumulation. The onset of accumulation is mainly influenced by light limitation after the winter solstice, and the transition from negative to positive biomass accumulation rates is driven by the rate of change in light availability rather than light itself.
Article
Multidisciplinary Sciences
Nastasia J. Freyria, Nathalie Joli, Connie Lovejoy
Summary: The study shows the seasonal dynamics of microbial communities in the North Water region, indicating that summer communities are more variable, while autumn communities favor undescribed dinoflagellates. Despite nutrient input, autumn chlorophyll concentrations remained low.
SCIENTIFIC REPORTS
(2021)
Article
Oceanography
C. Royer, A. V. Borges, J. Lapeyra Martin, N. Gypens
Summary: The study investigated the influence of abiotic and biotic factors on DMSP and DMSO concentrations in the Belgian coastal zone during two annual cycles in 2016 and 2018. While significant differences in Chl-a concentrations were observed between the two years, the DMSP and DMSO patterns remained consistent. This suggests that despite fluctuations in Chl-a levels, the overall DMSP and DMSO dynamics were stable over the two years.
CONTINENTAL SHELF RESEARCH
(2021)
Article
Microbiology
Greta Giljan, Carol Arnosti, Inga Kirstein, Rudolf Amann, Bernhard M. Fuchs
Summary: Marine heterotrophic bacteria play a significant role in global carbon cycling by utilizing phytoplankton-derived polysaccharides. The utilization patterns and rates of polysaccharides vary seasonally, along with changes in bacterial communities. This study found that the mode and speed of polysaccharide utilization differed between seasons, as did the bacterial clades involved.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Naeun Jo, Hyoung Sul La, Jeong-Hoon Kim, Kwanwoo Kim, Bo Kyung Kim, Myung Joon Kim, Wuju Son, Sang Heon Lee
Summary: This study investigated the biochemical compositions of POM in the late austral summer in the northwestern Ross Sea, finding variations in CHO, PRT, and amino acid contents in POM among different regions, influenced by phytoplankton biomass. Diatoms and Phaeocystis antarctica were identified as major bloom-forming taxa, impacting the biochemical compositions of POM significantly.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Limnology
Andreas Neumann, Justus E. E. van Beusekom, Annika Eisele, Kay-Christian Emeis, Jana Friedrich, Ingrid Kroencke, Ella Lu Logemann, Julia Meyer, Celine Naderipour, Ulrike Schueckel, Alexa Wrede, Michael L. Zettler
Summary: The research indicates that in the German Bight, pore-water advection and macrofaunal activity contribute equally to benthic oxygen uptake, and are influenced by factors such as physical parameters and biotransport.
LIMNOLOGY AND OCEANOGRAPHY
(2021)
Article
Environmental Sciences
Jaehong Kim, Hyoung Sul La, Jeong-Hoon Kim, Naeun Jo, Junbeom Lee, Bo Kyung Kim, Wuju Son, Kwanwoo Kim, Hyo-Keun Jang, Sanghoon Park, Hyeju Yoo, Jaesoon Kim, Jisoo Park, So Hyun Ahn, Sang Heon Lee
Summary: In this study, we investigated the seasonal variations in organic carbon composition and the relative abundance of each organic carbon in different algal groups in the Ross Sea. The results showed that the compositions of organic carbon were influenced by seasonal variations, physical characteristics, and phytoplankton community structure. Additionally, the environmental changes and phytoplankton community structures in the Ross Sea due to climate change could affect the organic carbon pool, which could determine the efficiency of the biological pump.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Oceanography
A. V. Chndrasekhararao, Siby Kurian, P. J. Vidya, Mangesh Gauns
Summary: The study investigated seasonal and inter-annual variations of phytoplankton characteristics in the NEAS using in situ data, highlighting the influence of SWM and NEM on phytoplankton productivity. Larger phytoplankton were supported during NEM while smaller groups were supported during late SWM, with a significant contribution of picoplankton in NEAS productivity, as influenced by ENSO and IOD. Comparison with US-JGOFS data showed comparable marker pigments over recent years but large inter-annual variability challenges long-term interpretation of phytoplankton composition changes. Long-term time-series observations in the Arabian Sea are recommended for understanding phytoplankton composition changes under climate change.
DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS
(2022)
Article
Microbiology
Samantha J. Gleich, Sarah K. Hu, Arianna I. Krinos, David A. Caron
Summary: Marine protists and their metabolic activities are influenced by physiochemical changes in the environment, such as mesoscale eddies. A metatranscriptomic study investigated the impact of eddies on protists at different depths in the oligotrophic ocean. The study found that eddy polarity affected protist communities, metabolism, and nutritional physiology, particularly at the lower euphotic zone.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Ecology
Nina Bartlau, Antje Wichels, Georg Krohne, Evelien M. Adriaenssens, Anneke Heins, Bernhard M. Fuchs, Rudolf Amann, Cristina Moraru
Summary: This study suggests that phages may modulate the succession of heterotrophic bacteria following phytoplankton blooms in spring. The diverse flavophages identified have the potential to affect their respective host populations in the North Sea microbial community, as stable components.
Article
Ecology
Luis H. Orellana, T. Ben Francis, Marcela Ferraro, Jan-Hendrik Hehemann, Bernhard M. Fuchs, Rudolf Amann
Summary: The study found that small, coccoid, free-living Verrucomicrobiota specialize in consuming fucose and rhamnose during spring algal blooms in the North Sea. These Verrucomicrobiota populations accounted for up to 8% of the bacterioplankton and actively expressed proteins critical for the consumption of fucosylated and sulfated polysaccharides during consecutive spring bloom events. Specialized metabolic pathways of Verrucomicrobiota could determine the fate of complex polysaccharides consumed during algae blooms, particularly those containing methyl pentose sugars.
Article
Biochemistry & Molecular Biology
Anna Kloska, Grzegorz M. Cech, Dariusz Nowicki, Monika Maciag-Dorszynska, Aleksandra E. Bogucka, Stephanie Markert, Doerte Becher, Katarzyna Potrykus, Paulina Czaplewska, Agnieszka Szalewska-Palasz
Summary: This study investigates the molecular adaptation mechanisms of marine bacteria to osmotic stress, focusing on adjustment at the proteome level. The results show that bacteria adjust at all levels of their biological processes in response to salinity challenges. The accumulation of DNA-binding proteins and increased polyamine uptake are suggested to play a role in protecting the nucleoid against changes in DNA topology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Jana Kizina, Sebastian F. A. Jordan, Gerrit Alexander Martens, Almud Lonsing, Christina Probian, Androniki Kolovou, Rachel Santarella-Mellwig, Erhard Rhiel, Sten Littmann, Stephanie Markert, Kurt Stueber, Michael Richter, Thomas Schweder, Jens Harder
Summary: In this study, the ultramicrobacterial anaerobic epibiont OP3 LiM living on Archaea and Bacteria was described. The study revealed the predatory metabolism of OP3 LiM cells through cell-to-cell contact and genomic analysis. The findings highlight the important role of OP3 LiM in the recycling and remineralization of microbial biomass.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Nicholas A. Pudlo, Gabriel Vasconcelos Pereira, Jaagni Parnami, Melissa Cid, Stephanie Markert, Jeffrey P. Tingley, Frank Unfried, Ahmed Ali, Neha J. Varghese, Kwi S. Kim, Austin Campbell, Karthik Urs, Yao Xiao, Ryan Adams, Duna Martin, David N. Bolam, Dorte Becher, Emiley A. Eloe-Fadrosh, Thomas M. Schmidt, D. Wade Abbott, Thomas Schweder, Jan Hendrik Hehemann, Eric C. Martens
Summary: Gut bacteria have the ability to digest seaweed polysaccharides, and this adaptation is a result of gene exchange and metabolic plasticity in the human gut microbiome.
CELL HOST & MICROBE
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Ramon Rossello-Mora, Konstantinos T. Konstantinidis, Rudolf Amann
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Ecology
Irena Beidler, Craig S. Robb, Silvia Vidal-Melgosa, Marie-Katherin Zuhlke, Daniel Bartosik, Vipul Solanki, Stephanie Markert, Dorte Becher, Thomas Schweder, Jan-Hendrik Hehemann
Summary: The presence of beta-mannan in diatom blooms suggests that marine microorganisms have a conserved enzymatic cascade for degrading beta-mannans from marine and terrestrial sources, highlighting the importance of this metabolic pathway in marine carbon cycling.
Article
Ecology
Bledina Dede, Taylor Priest, Wolfgang Bach, Maren Walter, Rudolf Amann, Anke Meyerdierks
Summary: In this study, the microbial community composition of hydrothermal plumes in the Northern Tonga arc and Northeastern Lau Basin was analyzed using multiple culture-independent techniques, with a focus on the autecology of Alcanivorax. Alcanivorax was found to dominate the community at two sites, despite no indication for hydrocarbon presence in the plumes. The high abundance and gene expression of Alcanivorax in these areas may be related to undiscovered hydrocarbon seepage from the seafloor, potentially resulting from recent volcanic activity.
Article
Biotechnology & Applied Microbiology
Stefan Brott, Ki Hyun Nam, Francois Thomas, Theresa Dutschei, Lukas Reisky, Maike Behrens, Hanna C. C. Grimm, Gurvan Michel, Thomas Schweder, Uwe T. T. Bornscheuer
Summary: Marine algae produce complex polysaccharides that can be degraded by marine heterotrophic bacteria using carbohydrate-active enzymes. The degradation of the red algal polysaccharide porphyran involves oxidative demethylation, catalyzed by cytochrome P450 monooxygenase and its redox partners. Genes encoding zinc-dependent alcohol dehydrogenases (ADHs) were found near the genes for key enzymes in this process. The physiological role of these marine ADHs was investigated and it was found that they are required for the utilization of a specific substrate, G6Me.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Ecology
Florian U. U. Moeller, Craig W. W. Herbold, Arno Schintlmeister, Maria Mooshammer, Cherie Motti, Bettina Glasl, Katharina Kitzinger, Faris Behnam, Margarete Watzka, Thomas Schweder, Mads Albertsen, Andreas Richter, Nicole S. S. Webster, Michael Wagner
Summary: Marine sponges play a critical role in marine ecosystems as filter-feeders and reef-builders, and they harbor dense communities of microbes. These microbes contribute to the processing of dissolved organic matter (DOM). A study using metaproteogenomics and laboratory incubations found that a dominant gammaproteobacterial symbiont in a marine sponge expressed a pathway for the import and dissimilation of taurine, a common metabolite in marine sponges. The symbiont also imported and used DMSP as a carbon and sulfur source. These findings highlight the importance of biogenic sulfur compounds in the interaction between marine sponges and their microbial symbionts.
Article
Environmental Sciences
Greta Reintjes, Anneke Heins, Cheng Wang, Rudolf Amann
Summary: Particulate organic matter is crucial in the marine carbon cycle for transporting organic carbon from surface to deep oceans. By analyzing particles and their microbiomes, we found significant differences between particle-attached and free-living bacterial communities in terms of composition, dynamics, and catabolic potential. We also discovered the dominance of specific bacterial taxa and their roles in glycan degradation, indicating niche diversification in particle-attached bacteria.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Microbiology
Joachim Vater, Le Thi Thanh Tam, Jennifer Jaehne, Stefanie Herfort, Christian Blumenscheit, Andy Schneider, Pham Thi Luong, Le Thi Phuong Thao, Jochen Blom, Silke R. Klee, Thomas Schweder, Peter Lasch, Rainer Borriss
Summary: Seventeen bacterial strains capable of suppressing plant pathogens have been isolated from healthy Vietnamese crops and identified as members of the Bacillus cereus group. Through comprehensive analysis, including whole-genome sequencing and gene mining, their potential as biocontrol agents has been proven. The study found that these plant-associated strains contain various antimicrobial peptides, most of which have been shown to enhance plant growth and suppress plant pathogens. However, the presence of virulence genes may restrict their application in agriculture.
Article
Ecology
Greta Giljan, Sarah Brown, C. Chad Lloyd, Sherif Ghobrial, Rudolf Amann, Carol Arnosti
Summary: Heterotrophic bacteria in the ocean invest in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake. Selfish bacteria change the odds in their favor by transporting polysaccharides without loss of hydrolysis products. Surprisingly, selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. The activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
ISME COMMUNICATIONS
(2023)
Correction
Microbiology
Tomeu Viver, Luis H. Orellana, Janet K. Hatt, Mercedes Urdiain, Sara Diaz, Michael Richter, Josefa Anton, Massimo Avian, Rudolf Amann, Konstantinos T. Konstantinidis, Ramon Rossello-Mora
ENVIRONMENTAL MICROBIOLOGY
(2022)
