Article
Chemistry, Multidisciplinary
Beibei Ma, Tianyu Lou, Tingting Wang, Ruiji Li, Jinhui Liu, Shangyue Yu, Hailuan Pei, Shiqiu Tian, Yilin Li, Yudong Guo, Zijian Wang, Zhaozhou Lin, Zhibin Wang, Jing Wang, Yingying Gao
Summary: This study utilized advanced chromatography-mass spectrometry techniques to reveal the metabolic fate of magnolol in rats, identifying numerous metabolites and offering new insights into its pharmacological effects and toxicity evaluation.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Plant Sciences
Haijuan Zhi, Zijia Zhang, Yanping Deng, Bingpeng Yan, Zhenwei Li, Wenyong Wu, Zijing Feng, Min Lei, Huali Long, Jinjun Hou, Dean Guo, Wanying Wu
Summary: Salvianolic acids have a special synergic effect with panax notoginsenosides in treating acute myocardial infarction (AMI) and have been developed into a new drug named Danqi Tongmai Tablet (DQTT). The network pharmacology-based analysis suggested that the therapeutic effects of DQTT on AMI might be related to the arachidonic acid pathway. DQTT treatment in AMI resulted in restoration of arachidonic acid metabolome with elevated anti-inflammatory metabolites and decreased pro-inflammatory lipids, alongside the down-regulation of pro-inflammatory cytokines.
Article
Food Science & Technology
Fanghua Guo, Rong Tsao, Chuyao Li, Xiaoya Wang, Hua Zhang, Li Jiang, Yong Sun, Hua Xiong
Summary: The study found that green pea hull extracts are rich in phenolic substances, such as quercetin, and have significant effects in alleviating DSS-induced colitis.
Article
Biochemical Research Methods
Jianqing Zhang, Qinghao Jin, Wenyong Wu, Xu Jin, Yaling An, Chen Liu, Wenlong Wei, Zhenwei Li, Changliang Yao, Shuai Yao, Yong Huang, Hua Qu, Jingyuan Song, Wanying Wu, De-an Guo
Summary: A force iteration molecular designing strategy was developed to systematically characterize and discover new protostane triterpenoids in herbal medicine. By combining different mass spectrometry techniques, new compounds were identified from Alisma Rhizoma, highlighting the importance of this integrated approach for comprehensive analysis of complex herbal components.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Irena Brcic Karaconji, Karlo Jurica, Uros Gasic, Aleksandra Dramicanin, Zivoslav Tesic, Dusanka Milojkovic Opsenica
Summary: This study compared the phenolic profiles, total phenolic content, and radical scavenging activity of strawberry tree leaves and fruits collected in two locations in Croatia. The results showed that both leaves and fruits contained a wide range of phenolic compounds and exhibited strong antioxidant activity, indicating the potential health-promoting properties of the strawberry tree.
Article
Agriculture, Multidisciplinary
Fanghua Guo, Rong Tsao, Xiaoya Wang, Li Jiang, Yong Sun, Hua Xiong
Summary: The study analyzed the chemical profiles, metabolites, and organ distribution of yellow pea hull (YPH) extract, as well as their in vivo antioxidant activities in rats using the D-gal model. Results showed that the distribution of these polyphenol metabolites in plasma and organs may have positive effects on antioxidant enzymes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Xiaoya Sun, Shujuan Xue, Yongxia Cui, Meng Li, Suiqing Chen, Jingyang Yue, Zhining Gao
Summary: Corni Fructus (CF) is a widely used traditional medicine and food, but its chemical profile and the impact of storage periods on indicative components have not been systematically studied. In this study, UHPLC-LTQ-Orbitrap-MS was used to investigate the fragmentation behaviors of multiple compounds from CF and the content variety of its indicative components for different storage periods. A total of 130 components, including 47 potentially new compounds, were identified. The storage period studies indicated that the contents of 19 indicative components in CF changed differently with the prolongation of the storage period.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Plant Sciences
Uros Gasic, Tijana Banjanac, Branislav Siler, Jelena Bozunovic, Milica Milutinovic, Neda Anicic, Slavica Dmitrovic, Marijana Skoric, Jasmina Nestorovic Zivkovic, Luka Petrovic, Milos Todorovic, Suzana Zivkovic, Dragana Matekalo, Biljana Filipovic, Tamara Lukic, Danijela Misic
Summary: This study aimed to investigate the variation in the composition of methanol-soluble metabolites in the leaves of three Digitalis species. Through untargeted profiling, 115 compounds were identified, with 16 compounds being quantified. The composition of the metabolites showed differences between the species, with D. grandiflora having unique compounds. However, there was limited variation in the content of the metabolites within species, indicating conservation across populations. This metabolomics approach can be valuable for understanding the relationships among Digitalis taxa.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Applied
Lan Yu, Xiaolu Hu, Rongrong Xu, Yinying Ba, Xiaoqing Chen, Xing Wang, Bing Cao, Xia Wu
Summary: The study identified diverse amide alkaloids in different parts of Piper nigrum L., with piperine being the most abundant compound known for its neuroprotective activities.
Article
Food Science & Technology
Yangzheng He, Li Peng, Hua Xiong, Wenqiang Liu, Hua Zhang, Xin Peng, Xuemei Zhu, Fanghua Guo, Yong Sun
Summary: Durian is a nutritious tropical fruit with various health benefits. The study focuses on the durian shell, which is often discarded as waste. The research identifies compounds and metabolites in the durian shell extract and explores its antioxidant properties and potential mechanisms of action.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Pharmacology & Pharmacy
Jinli Hou, Xiang Zhou, Ping Wang, Chunhui Zhao, Yuewen Qin, Feng Liu, Liping Yu, Haiyu Xu
Summary: Purslane seed can be used as an adjunctive and alternative therapy for diabetes mellitus, with hypoglycemic effects and multi-faceted actions such as anti-hyperlipidemia and immunity regulation. Ultra-high-pressure liquid chromatography and Integrative Pharmacology-based Research Platform were used to analyze the active components and molecular mechanisms of purslane seed in diabetes.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Fanghua Guo, Rong Tsao, Chuyao Li, Xiaoya Wang, Hua Zhang, Li Jiang, Yong Sun, Hua Xiong
Summary: The study found that pea hull polyphenols can be continuously released during digestion and effectively absorbed, exerting protective and anti-inflammatory effects on the intestinal barrier.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jing Li, Xinghua Li, Xiaohang Zhou, Le Yang, Hui Sun, Ling Kong, Guangli Yan, Ying Han, Xijun Wang
Summary: This study established an efficient strategy for the comprehensive in vivo metabolite profiling of DMU-212. Using UHPLC-Q/Orbitrap/LTQ MS in the AcquireX(TM) intelligent data acquisition mode, the metabolites of DMU-212 in vivo were analyzed and identified. A total of 63 metabolites of DMU-212 were tentatively identified.
Article
Chemistry, Applied
Chagam Koteswara Reddy, Su Young Son, Choong Hwan Lee
Summary: The study demonstrates that debranched maize starch can form inclusion complexes with catechin-rich green tea ethanolic extract, showing high recovery yield and stability.
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Applied
Durda D. Krstic, Petar M. Ristivojevic, Uros M. Gasic, Mila Lazovic, Milica M. Fotiric Aksic, Jasminka Milivojevic, Gertrud E. Morlock, Dusanka M. Milojkovic-Opsenica, Jelena D. Trifkovic
Summary: This research characterized and differentiated the phytochemical features of cultivated berry seeds using phenolic profiling and identified biomarkers for authentication of botanical origin. A total of 103 phenolic compounds were identified, proving the efficacy of phenolic profiles as a powerful tool in distinguishing different plant origins of berry seeds.
Article
Pharmacology & Pharmacy
Wen-Long Wei, Hao-Jv Li, Wen-Zhi Yang, Hua Qu, Zhen-Wei Li, Chang-Liang Yao, Jin-Jun Hou, Wan-Ying Wu, De-An Guo
Summary: This study established an effective strategy to comprehensively characterize the metabolites of bufadienolides in vivo, deduce the metabolic pathways, and describe the metabolic profiles. The strategy successfully identified 147 metabolites and elucidated the main metabolic pathways of bufadienolides in vivo.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Applied
Jinjun Hou, Zijia Zhang, Linlin Zhang, Wenyong Wu, Yong Huang, Zhengwei Jia, Lihong Zhou, Lei Gao, Huali Long, Min Lei, Wanying Wu, De-an Guo
Summary: This study utilized advanced mass spectrometry techniques to visualize the spatial distribution of lipids in eight edible nuts for the first time, highlighting the differences in lipid components among different types of nuts. The results provide a deeper understanding of the diverse metabolite profiles in nuts and their relationship to health benefits.
Article
Biochemistry & Molecular Biology
Jiangyan Huo, Min Lei, Yang Zhou, Xianchun Zhong, Yameng Liu, Jinjun Hou, Huali Long, Zijia Zhang, Menghua Tian, Cen Xie, Wanying Wu
Summary: Two homogeneous polysaccharides, GEP-3 and GEP-4, were extracted from Gastrodia data, a traditional Chinese medicine. GEP-3 was identified as 1,4-glucan while GEP-4 was characterized as a novel polysaccharide. Bioactivity test indicated that both GEP-3 and GEP-4 significantly promoted the growth of Akkermansia muciniphila.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemical Research Methods
Haodong Zhu, Xingdong Wu, Jiangyan Huo, Jinjun Hou, Huali Long, Zijia Zhang, Bing Wang, Menghua Tian, Kaixian Chen, De'an Guo, Min Lei, Wanying Wu
Summary: A comprehensive strategy integrating ion mobility mass spectrometry with offline two-dimensional liquid chromatography has been developed to enhance chemical composition characterization and metabolite discernment. This approach allows for efficient and detailed analysis of complex food and drug samples, improving identification of compounds and distinguishing isomers. The technique was successfully applied to characterize 272 compounds in Gastrodia Rhizoma, demonstrating its capacity to support the study of chemical diversity in Traditional Chinese Medicines.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Lei Gao, Zijia Zhang, Zijin Feng, Wenlong Wei, Wenyong Wu, Haijuan Zhi, Yanping Deng, Huali Long, Min Lei, Jinjun Hou, De-an Guo, Wanying Wu
Summary: This study established a sensitive, reproducible, and rapid liquid chromatography method with tandem mass spectrometry detection for simultaneous quantitative analysis of 16 neurotransmitters and their metabolites in rats. The intervention with Uncaria, a traditional Chinese medicine, showed significant effects on regulating the levels of neurotransmitters in hypertensive rats, indicating its potential in treating symptoms related to the nervous system. The results suggest a potential relationship between changes in plasma neurotransmitters and hypertension pathogenesis, providing valuable insights into the mechanisms of Uncaria in lowering blood pressure.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Haoran Yin, Hui Ni, Linlin Zhang, Wenyong Wu, Xingdong Wu, Zijia Zhang, Huali Long, Min Lei, Jinjun Hou, Wanying Wu
Summary: This study identified two potential chemical markers for discriminating the processing procedures of wine-processed Angelica sinensis and proposed a strategy for research on other decoction pieces based on chemical analysis and investigation.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Xingdong Wu, Huali Long, Feifei Li, Wenyong Wu, Jing Zhou, Chen Liu, Jinjun Hou, Wanying Wu, Dean Guo
Summary: The study utilized advanced techniques to differentiate the components of Cinnamomum cassia Bark and Cinnamomum verum bark, providing new insights for discovering quality markers and identifying unknown components in natural products.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Huali Long, Shuai Yao, Wenshuai Tian, Jinjun Hou, Min Lei, Zijia Zhang, Dean Guo, Wanying Wu
Summary: This study developed a simple and effective method to identify Fraxini Cortex from different origins using multi-mode fingerprint combined with chemometrics. The method was able to authenticate and quality assess the Fraxini Cortex, and distinguish its two original medicinal materials as well as three adulterants, providing a potential differentiation method for other medicinal and economic plants.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Lei Gao, Zijia Zhang, Wenyong Wu, Yanping Deng, Haijuan Zhi, Huali Long, Min Lei, Jinjun Hou, Wanying Wu, De-an Guo
Summary: This study quantitatively investigated the distribution of Uncaria alkaloids in rat brains using mass spectrometry imaging, revealing the distribution patterns of different alkaloids and the novel detection of Uncaria alkaloids in the pineal gland. The enrichment phenomenon of these alkaloids in the pineal gland has significant implications for future pharmacodynamic studies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Xingdong Wu, Jinjun Hou, Zijia Zhang, Lingmin Chen, Hui Ni, Yong Qian, Wenyong Wu, Huali Long, Linlin Zhang, Feifei Li, Min Lei, Yong Huang, Dean Guo, Wanying Wu
Summary: This study established an offline two-dimensional separation system combined with high-resolution mass spectrometry data acquisition for in-depth exploration and comparison of chemical components in Lilium. A total of 331 components were identified, with phenylpropanoid derivatives and steroidal saponins being the most abundant. Furthermore, sulfur derivatives and steroidal alkaloids were characterized in Lilium for the first time, providing valuable information for differentiating types of components and enhancing the edible and medicinal values of Lilium.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Pharmacology & Pharmacy
Lei Gao, Xinqin Kong, Wenyong Wu, Zijin Feng, Haijuan Zhi, Zijia Zhang, Huali Long, Min Lei, Jinjun Hou, Wanying Wu, De-an Guo
Summary: This study investigated the hypotensive effect of Uncaria rhynchophylla (UR) extract and its regulatory role in metabolic pathways. The results showed that UR extract could effectively lower blood pressure in spontaneously hypertensive rats (SHRs) for 8 weeks. Non-targeted and targeted metabolomics analyses revealed the reorganization of metabolic pathways and the restoration of cardiovascular active compounds' functions after UR administration. The inhibition of soluble epoxide hydrolase (sEH) enzyme activity and up-regulation of vasodilators epoxyeicosatrienoic acids (EETs) were identified as contributors to the anti-hypertension effect of UR.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Wenyong Wu, Jinjun Hou, Zijia Zhang, Feifei Li, Rong Zhang, Lei Gao, Hui Ni, Tengqian Zhang, Huali Long, Min Lei, Bing Shen, Jun Yan, Ruimin Huang, Zhongda Zeng, Wanying Wu
Summary: This study presents a novel method for objectively evaluating the image quality of hyperspectral images. The method is validated by analyzing data from human prostate cancer and mouse brain tissue, demonstrating its applicability. The method utilizes information entropy and contrast to assess image quality and reduces high-dimensional data to three-dimensional embeddings for evaluation, resulting in satisfactory visualization results.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Lihong Zhou, Hui Ni, Linlin Zhang, Wenyong Wu, Tengqian Zhang, Qi Su, Jing Zhou, Huali Long, Jinjun Hou, Jiyu Gong, Wanying Wu
Summary: In this study, a QAMS method was developed using HILIC-HPLC-ELSD to determine the seven fructooligosaccharides of Morinda officinalis. A scientific calculation method for RCF in LC-ELSD method was identified. The QAMS method showed good linearity and accuracy for the determination of fructooligosaccharides. Furthermore, the content of the fructooligosaccharides could differentiate Morinda officinalis and its processed products.
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Yuhan Cao, Jingying Shi, Luyao Song, Junjiu Xu, Henglei Lu, Jianhua Sun, Jinjun Hou, Jing Chen, Wanying Wu, Likun Gong
Summary: This study identified the chemical composition of ZXBZ decoction and discovered its hepatoprotective effect in a NAFLD mouse model. Metabolomics and transcriptomics analyses showed that ZXBZ decoction may regulate lipid and glucose metabolism, and influence proteins involved in lipid biosynthesis, catabolism, and transport.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Jin-jun Hou, Zi-jia Zhang, Wen-yong Wu, Qing-qing He, Teng-qian Zhang, Ya-wen Liu, Zhao-jun Wang, Lei Gao, Hua-li Long, Min Lei, Wan-ying Wu, De-an Guo
Summary: Natural products and their structural analogs play a major role in novel drug development, but few molecular imaging technologies can detect multiple compounds simultaneously. Mass spectrometry imaging has been instrumental in discovering natural products and evaluating their applications, providing a visualization tool for drug development.
ACTA PHARMACOLOGICA SINICA
(2022)
