Article
Biochemistry & Molecular Biology
Noelia Carballeda Sangiao, Susana Chamorro, Sonia de Pascual-Teresa, Luis Goya
Summary: The study confirms the significant protective effect of phenolic compounds and epicatechin in aqueous extract of cocoa powder against oxidative stress on neuronal-like cells under physiological conditions.
Article
Biochemistry & Molecular Biology
Grzegorz Bartosz, Natalia Pienkowska, Kacper Kut, Bogumil Cieniek, Ireneusz Stefaniuk, Izabela Sadowska-Bartosz
Summary: Nitroxides are stable synthetic free radicals that have potential as antioxidants. This study investigated the effects of low concentrations of three nitroxides on chemical systems and cells. The results showed that nitroxides can affect the cellular redox equilibrium and other biochemical parameters, indicating their potential as antioxidants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Neha Kulkarni, Rajitha Gadde, Kuljeet S. Gugnani, Nguyen Vu, Claude Yoo, Rohan Zaveri, Swati Betharia
Summary: ACDT exhibits significant neuroprotection against Mn-induced cytotoxicity by enhancing cellular antioxidant defense stores, reducing Mn-induced oxidative stress, and inhibiting Mn cellular uptake as an additional neuroprotective mechanism. These effects suggest dithiolethiones like ACDT could be a potential novel therapeutic option against heavy metal neurotoxicity.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Chemistry, Applied
Liang Zheng, Wouter Bakker, Ignacio Miro Estruch, Frances Widjaja, Ivonne M. C. M. Rietjens
Summary: This study characterizes the scavenging capacity of glutathione (GSH) and kaempferol for methylglyoxal (MGO), finding that kaempferol is a better scavenger for food-borne MGO, while GSH is more dominant for intracellular MGO protection.
Article
Oncology
Shuyan Zhang, Xinyue Zhang, Xuanzhong Wang, Chen Li, Chuan He, Tianfei Luo, Pengfei Ge
Summary: This study investigated the treatment effect of maltol on ischemia-induced neuron damage using an oxygen and glucose deprivation (OGD) model in SH-SY5Y cells. The results showed that maltol protected the cells against OGD-induced chromatinolysis by inhibiting two reactive oxygen species (ROS)-regulated pathways and maintaining the levels of xCT and catalase.
MOLECULAR MEDICINE REPORTS
(2023)
Article
Toxicology
Zhaofei Li, Fengjie Tian, Hongfang Ban, Shuangshuang Xia, Lixia Cheng, Xueke Ren, Yi Lyu, Jinping Zheng
Summary: This study investigates the effects of PM2.5 exposure on mitochondrial energy metabolism and oxidative damage in SH-SY5Y cells. The results show that PM2.5 causes disruption in cellular energy metabolism, oxidative damage, and apoptosis, which can be partially recovered by BHA.
TOXICOLOGY LETTERS
(2022)
Article
Endocrinology & Metabolism
Izabel Cristina Custodio de Souza, Renata Cristina Bertolini Gobbo, Fhelipe Jolner Souza de Almeida, Matheus Dargesso Luckachaki, Marcos Roberto de Oliveira
Summary: The study demonstrated that pretreatment with carnosic acid (CA) can promote mitochondrial protection in human neuroblastoma SH-SY5Y cells exposed to MG by reducing lipid peroxidation and protein carbonylation and nitration, preventing dysfunction of Complexes I and V, decline in ATP levels, and loss of MMP, as well as decreasing the production of superoxide. These protective effects were mediated by upregulating glutathione synthesis through the PI3K/Akt/Nrf2/gamma-GCL/GSH axis.
METABOLIC BRAIN DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Haya Alrashidi, Simon Eaton, Simon Heales
Summary: This study investigated the differences in biochemical processes between proliferative and differentiated phenotypes of SH-SY5Y cells, a commonly used model for Parkinson's disease. Significant variations were found in extracellular dopamine metabolism, glutathione levels, and lysosomal enzyme activity between the two phenotypes. Despite some similarities, careful characterization of relevant disease pathways is crucial when using the same cell line but in different differentiation states.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Natalia Pienkowska, Margaret Fahnestock, Crystal Mahadeo, Izabela Zaborniak, Pawel Chmielarz, Grzegorz Bartosz, Izabela Sadowska-Bartosz
Summary: This study found that overexpression of tau protein leads to changes in the redox status of human neuroblastoma SH-SY5Y cells. Tau-overexpressing cells showed elevated levels of reactive oxygen species (ROS) and glutathione, indicating their heightened sensitivity to oxidative stress. The use of nitroxide-containing nanoparticles mitigated tau-protein-induced oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lucie Valek, Irmgard Tegeder
Summary: Knockdown of nucleoredoxin (NXN) in neuronal cells leads to increased proliferation, elevated basal respiratory rates, and a similar relative change in oxygen consumption compared to control cells under mitochondrial stressors. Moreover, NXN-deficient cells show increased expression of redox-sensitive heat shock proteins and autophagy markers, indicating higher levels of self-renewal and autophagy.
Article
Cardiac & Cardiovascular Systems
Qinfeng Li, Chao Li, Abdallah Elnwasany, Gaurav Sharma, Yu A. An, Guangyu Zhang, Waleed M. Elhelaly, Jun Lin, Yingchao Gong, Guihao Chen, Meihui Wang, Shangang Zhao, Chongshan Dai, Charles D. Smart, Juan Liu, Xiang Luo, Yingfeng Deng, Lin Tan, Shuang-Jie Lv, Shawn M. Davidson, Jason W. Locasale, Philip L. Lorenzi, Craig R. Malloy, Thomas G. Gillette, Matthew G. Vander Heiden, Philipp E. Scherer, Luke I. Szweda, Guosheng Fu, Zhao V. Wang
Summary: PKM1 plays a crucial role in maintaining a homeostatic response in the heart under hemodynamic stress, protecting against cardiac dysfunction and fibrosis caused by pressure overload. Furthermore, PKM1 is essential for enhancing glycolytic flux, mitochondrial respiration, and ATP production under pressure overload at a mechanistic level.
Article
Biochemistry & Molecular Biology
Angela Maria Casaril, Natalia Segatto, Lucas Simoes, Julia Paschoal, Micaela Domingues, Beatriz Vieira, Fernanda S. S. Sousa, Eder Joao Lenardao, Fabiana K. Seixas, Tiago Collares, Lucielli Savegnago
Summary: The study demonstrated that CMI protects dopaminergic neurons from H2O2-induced stress by lowering ROS levels and boosting the glutathione system, suggesting its potential clinical application in treating nervous system diseases associated with oxidative stress.
NEUROCHEMICAL RESEARCH
(2021)
Article
Food Science & Technology
Carmen Rodriguez-Rivera, Maria Dolores Perez-Carrion, Lucia Casariego Olavarria, Luis F. Alguacil, Maria Jose Polanco Mora, Carmen Gonzalez-Martin
Summary: Food-related disorders are on the rise in developed societies, with a growing focus on the psychological component of these disorders. Research has shown that overnourishment with high-fat diets and perinatal undernourishment in mice are associated with increased motivation toward food intake. The protein clusterin (CLU) is overexpressed in the nucleus accumbens of over-fed rats and those suffering from chronic undernutrition, and its increase has been observed in obese patients with food addiction, suggesting its implication in this eating disorder.
FOOD & NUTRITION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Andrila E. Collins, Tarek M. Saleh, Bettina E. Kalisch
Summary: This study aimed to assess the cytoprotective effects of the novel antioxidant compound VANL-100 in a cellular model of beta-amyloid (A beta)-induced toxicity. The results demonstrate that VANL-100 significantly attenuated A beta-induced cell death, indicating its cytoprotective effects against A beta-induced toxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Seda Onder, Lawrence M. Schopfer, Wei Jiang, Ozden Tacal, Oksana Lockridge
Summary: It has been found that SH-SY5Y cells express active AChE and BChE, but these proteins do not mature into glycosylated tetramers.
Article
Multidisciplinary Sciences
Andrei Timotin, Mathieu Cinato, Frederic Boal, Sebastien Dejean, Rodica Anesia, Oleg Arnaut, Christine Lagente, Jerome Roncalli, Franck Desmoulin, Helene Tronchere, Oksana Kunduzova
SCIENTIFIC REPORTS
(2018)
Article
Clinical Neurology
Carla Cirillo, Alice Le Friec, Isabelle Frisach, Robert Darmana, Lorenne Robert, Franck Desmoulin, Isabelle Loubinoux
FRONTIERS IN NEUROLOGY
(2018)
Meeting Abstract
Oncology
F. Tensaouti, J. Bailleul, E. Martin, F. Desmoulin, S. Ken, J. Desrousseaux, L. Vieillevigne, J. Lotterie, V. Lubrano, I. Catalaa, G. Noel, G. Truc, M. Sunyach, M. Charissoux, N. Magne, P. Auberdiac, T. Filleron, P. Peran, E. Cohen-Jonathan Moyal, A. Laprie
RADIOTHERAPY AND ONCOLOGY
(2019)
Article
Cardiac & Cardiovascular Systems
Manon Barutaut, Pauline Fournier, William F. Peacock, Maria Francesca Evaristi, Celine Caubere, Annie Turkieh, Franck Desmoulin, Luc W. M. Eurlings, Sandra van Wijk, Hans-Peter Brunner-La Rocca, Javed Butler, Francois Koukoui, Camille Dambrin, Serge Mazeres, Servane Le Page, Clement Delmas, Michel Galinier, Christian Jung, Fatima Smih, Philippe Rouet
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2020)
Review
Endocrinology & Metabolism
Carla Cirillo, Nabila Brihmat, Evelyne Castel-Lacanal, Alice Le Friec, Marianne Barbieux-Guillot, Nicolas Raposo, Jeremie Pariente, Alain Viguier, Marion Simonetta-Moreau, Jean-Francois Albucher, Jean-Marc Olivot, Franck Desmoulin, Philippe Marque, Francois Chollet, Isabelle Loubinoux
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2020)
Correction
Clinical Neurology
Alice Le Friec, Franck Desmoulin, Boris Demain, Carole Davoust, Lorenne Robert, Tanguy Duval, Florence Remy, Carla Cirillo, Isabelle Loubinoux
TRANSLATIONAL STROKE RESEARCH
(2021)
Article
Clinical Neurology
Alice Le Friec, Franck Desmoulin, Boris Demain, Carole Davoust, Lorenne Robert, Tanguy Duval, Florence Remy, Carla Cirillo, Isabelle Loubinoux
Summary: This study introduced a new method to induce focal brain damage targeting the motor cortex in marmoset monkeys, resulting in long-lasting motor deficits. The MRI signature indicated Wallerian degeneration and remote injury of motor tracts, suggesting that this model could be useful for testing post-stroke recovery therapies, particularly in the chronic phase.
TRANSLATIONAL STROKE RESEARCH
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Fatima Tensaouti, Franck Desmoulin, Julia Gilhodes, Elodie Martin, Soleakhena Ken, Jean-Albert Lotterie, Georges Noel, Gilles Truc, Marie-Pierre Sunyach, Marie Charissoux, Nicolas Magne, Vincent Lubrano, Patrice Peran, Elizabeth Cohen-Jonathan Moyal, Anne Laprie
Summary: In this study, automatic quality control of glioblastoma MRSI data using random forest analysis was successfully implemented, demonstrating the ability to distinguish between good and poor-quality spectra. The research revealed a novel set of MRSI signal features closely correlated with spectral quality.
MAGNETIC RESONANCE IN MEDICINE
(2022)
Review
Clinical Neurology
Sirine Hacene, Alice Le Friec, Franck Desmoulin, Lorenne Robert, Nina Colitti, Juliette Fitremann, Isabelle Loubinoux, Carla Cirillo
Summary: Cell therapy is a promising strategy in regenerative medicine, but the lack of valid cell sources is a limiting factor. The gastrointestinal tract, specifically the enteric nervous system (ENS), has been identified as a valuable source of nerve cells for therapy. Enteric glial cells, with their neurotrophic and neurogenic potential, have shown promise in treating intestinal neuropathies. Additionally, the ENS has therapeutic potential for brain repair, particularly in cases of stroke. The urgency for alternative treatments to rehabilitation in chronic stroke patients is evident. This review explores the pathophysiology of stroke and discusses current therapeutic strategies, with a focus on cell- and biomaterial-based approaches. The anatomical and functional characteristics of the ENS, particularly glial cells, are also presented, highlighting their neurotrophic and neurogenic properties. Preliminary data on a possible therapeutic approach combining ENS-derived cells and a biomaterial are discussed.
Article
Neurosciences
Nina Colitti, Franck Desmoulin, Alice Le Friec, Wafae Labriji, Lorenne Robert, Amandine Michaux, Fabrice Conchou, Carla Cirillo, Isabelle Loubinoux
Summary: This study demonstrates the importance of long-term treatment with nerve growth factor (NGF) delivered intranasally in promoting neurogenesis and tissue reconstruction after stroke. However, it also indicates that NGF treatment may limit motor recovery. Additionally, the study establishes the use of MRI as a biomarker for evaluating neurogenesis and tissue reconstruction.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Wafae Labriji, Julien Clauzel, Jean-Louis Mestas, Maxime Lafond, Cyril Lafon, Anne-Sophie Salabert, Lydiane Hirschler, Jan M. Warnking, Emmanuel L. Barbier, Isabelle Loubinoux, Franck Desmoulin
Summary: The purpose of this study was to investigate the effect of Blood Brain Barrier (BBB) opening using ultrasound combined with microbubbles injection on cerebral blood flow in rats. The results showed that BBB opening induced by microbubble intravenous injection led to transient hypoperfusion, which shared some similarities with cortical spreading depression phenomenon.
MAGNETIC RESONANCE IN MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Marjorie Yon, Laure Gibot, Stephane Gineste, Pascale Laborie, Christian Bijani, Christophe Mingotaud, Olivier Coutelier, Franck Desmoulin, Carine Pestourie, Mathias Destarac, Diana Ciuculescu-Pradines, Jean-Daniel Marty
Summary: Due to the potential life-threatening side effects caused by specific antibodies to poly(ethylene glycol) (PEG), it is necessary to develop alternatives to PEGylated copolymers for treatment and diagnostic methods. A new approach involves block copolymers containing a poly(N-vinyl-2-pyrrolidone) (PVP) segment as vectors without PEG block. A synthesized poly(acrylic acid)-block-poly(N-vinyl-2-pyrrolidone) (PAA-b-PVP) copolymer, when combined with lanthanide cations (Gd3+, Eu3+, Y3+), forms stable hybrid polyion complexes that prevent lanthanide cytotoxicity and enhance in vitro cell penetration, resulting in enhanced T-1 MRI contrast when administered to mice intravenously without leaching of gadolinium ions.
Article
Oncology
Ingrid Sidibe, Fatima Tensaouti, Julia Gilhodes, Bastien Cabarrou, Thomas Filleron, Franck Desmoulin, Soleakhena Ken, Georges Noel, Gilles Truc, Marie Pierre Sunyach, Marie Charissoux, Nicolas Magne, Jean -Albert Lotterie, Margaux Roques, Patrice Peran, Elizabeth Cohen-Jonathan Moyal, Anne Laprie
Summary: This study investigates the feasibility of using a multiapproach analysis to distinguish true tumor progression (TP) from pseudoprogression (PSP) in glioblastoma patients. The results suggest that three spectroscopic ratios can differentiate PSP from TP, and tumor volume at relapse is the most predictive factor in the multivariate analysis. Patients with PSP have a higher overall survival compared to TP patients.
RADIOTHERAPY AND ONCOLOGY
(2023)
Article
Oncology
Fatima Tensaouti, Franck Desmoulin, Julia Gilhodes, Margaux Roques, Soleakhena Ken, Jean -Albert Lotterie, Georges Noel, Gilles Truc, Marie -Pierre Sunyach, Marie Charissoux, Nicolas Magne, Vincent Lubrano, Patrice Peran, Elizabeth Cohen-Jonathan Moyal, Anne Laprie
Summary: This study aimed to identify different components of glioblastoma subtypes using a large MRSI dataset and determine their association with progression-free survival. The results showed that pre-radiotherapy MRSI can reveal tumor heterogeneity and clusters with metabolic abnormalities and high lactate are predictive of PFS.
RADIOTHERAPY AND ONCOLOGY
(2023)
Meeting Abstract
Neurosciences
C. Cirillo, S. Lionnet, A. Le Friec, L. Robert, F. Desmoulin, I. Loubinoux