Article
Cell & Tissue Engineering
Peng-Peng Kuang, Xiao-Qing Liu, Chan-Gu Li, Bi-Xin He, Ying-Chun Xie, Zi-Cong Wu, Cheng-Lin Li, Xiao-Hui Deng, Qing-Ling Fu
Summary: This study investigated the therapeutic advantages of genetically engineered MSCs overexpressing IL-10 for the treatment of allergic airway inflammation. The findings showed that IL-10-MSCs significantly reduced inflammation and improved symptoms in mouse models of allergic airway inflammation. The study provides solid support for the use of genetically engineered MSCs as a potential novel therapy for allergic airway inflammation.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Immunology
Karin Peters, Marcus Peters
Summary: The text discusses the role of c-type lectin receptors (CLR) in the immune system, highlighting their ability to recognize both foreign and endogenous molecules and mediate inhibitory signals. While CLR have been associated with allergic inflammation, studies also suggest the involvement of sugar moieties in immune regulation. The identification of microbial substances responsible for allergy protection remains a topic of interest.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Mohammad Gholami, Khodayar Ghorban, Mahvash Sadeghi, Maryam Dadmanesh, Negin Hosseini Rouzbahani, Sajad Dehnavi
Summary: This review summarizes the potential therapeutic effects of mesenchymal stem cells (MSCs) on allergic airway inflammations. The studies suggest that MSCs can modulate airway inflammation, affect inflammatory cell infiltration, balance Th1/Th2 cellular responses, disrupt humoral responses, regulate Th17/Treg cell balance, and modulate macrophage and dendritic cell function.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Dagmar Srutkova, Hana Kozakova, Tereza Novotna, Sabina Gorska, Petra Petr Hermanova, Tomas Hudcovic, Tereza Svabova, Marek Sinkora, Martin Schwarzer
Summary: The high prevalence of allergic diseases in industrialized countries is believed to be related to changes in microbial exposure. The administration of exopolysaccharide (EPS) from Lacticaseibacillus (L.) rhamnosus LOCK900 induces immune responses in the lungs of mice, leading to a reduction in allergic airway inflammation and Th2 cytokine response in sensitized individuals. Furthermore, EPS treatment increases the production of IgA in the lungs of sensitized mice. These findings suggest that EPS derived from L. rhamnosus LOCK900 could be a safe candidate for preventing the development of allergic symptoms in sensitized individuals.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Immunology
Elke Korb, Mirjana Drinic, Angelika Wagner, Nora Geissler, Aleksandra Inic-Kanada, Roman Peschke, Anja Joachim, Ursula Wiedermann, Irma Schabussova
Summary: TLA, a protein from Toxoplasma gondii, has shown to have a suppressive effect on allergic inflammation in mice, depending on the timing of application in relation to sensitization. Deglycosylated TLA was able to reduce type 2 responses ex vivo, indicating a significant role of carbohydrates in immunomodulation.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Nicolas Degauque, Alain Haziot, Sophie Brouard, Nuala Mooney
Summary: In the inflammation and infection caused by SARS-CoV-2, endothelial cells play a crucial role in regulating the recruitment of immune cells and interacting with monocytes, T cells, and B cells. Endothelial cells function as an integrative and active platform for immune responses, providing opportunities for therapeutic intervention.
Article
Pharmacology & Pharmacy
Pablo Rayff da Silva, Renan Fernandes do Espirito Santo, Camila de Oliveira Melo, Fabio Emanuel Pachu Cavalcante, Thassia Borges Costa, Yasmim Vilarim Barbosa, Yvnni M. S. de Medeiros e Silva, Natalia Ferreira de Sousa, Cristiane Flora Villarreal, Ricardo Olimpio de Moura, Vanda Lucia dos Santos
Summary: The compound (E)-2-cyano-N,3-diphenylacrylamide (JMPR-01) shows strong anti-inflammatory potential and could be a potential drug for the treatment of inflammatory diseases.
Article
Biochemistry & Molecular Biology
Mauricio Castillo-Salazar, Fausto Sanchez-Munoz, Rashidi Springall del Villar, Gabriel Navarrete-Vazquez, Adrian Hernandez-DiazCouder, Carlos Mojica-Cardoso, Sara Garcia-Jimenez, Cairo Toledano-Jaimes, German Bernal-Fernandez
Summary: The study demonstrated that nitazoxanide has immunomodulatory effects on peripheral blood mononuclear cells from patients with type 2 diabetes, inhibiting T lymphocyte proliferation, reducing various cytokine concentrations, and regulating the M1/M2 ratio through miRNA expression modulation.
Article
Food Science & Technology
Pei-Ying Huang, Chia-Chien Hsieh
Summary: This study investigated the mechanism of lunasin in regulating immune functions of RAW264.7 macrophages under obesity-related conditions. The findings showed that lunasin protected the vitality of macrophages, suppressed leptin secretion, and reduced inflammation markers in LPS-stimulated co-culture. However, it had opposing effects in adipocyte-conditioned medium and co-culture without LPS. Additionally, lunasin enhanced phagocytosis in both LPS and adipocyte-conditioned medium challenges.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Review
Pharmacology & Pharmacy
Safura Pournajaf, Leila Dargahi, Mohammad Javan, Mohammad Hossein Pourgholami
Summary: Fingolimod is an effective therapy for disease progression, reducing T-cell numbers, suppressing inflammation, and potentially treating various pathological conditions. It modulates multiple molecular pathways and has shown potential therapeutic effects in immunomodulatory diseases, CNS injuries, Alzheimer's disease, Parkinson's disease, epilepsy, and cancer.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Nutrition & Dietetics
Samuel Adler, Wyatt Olsen, Bryna Rackerby, Rachel Spencer, David C. Dallas
Summary: This study investigated the effects of whey protein isolate (WPI) supplementation on inflammatory markers in healthy older adults. The results showed that WPI supplementation did not significantly alter most inflammatory markers, but could alter the concentrations of some inflammatory markers.
Review
Nutrition & Dietetics
Alberto Caballero-Garcia, Alfredo Cordova-Martinez, Nestor Vicente-Salar, Enrique Roche, Daniel Perez-Valdecantos
Summary: Vitamin D plays a crucial role in immune modulation, enhancing macrophage functions and modulating muscle inflammatory response and protein synthesis. It is particularly important in cases of deficiency or high muscular demand.
Article
Immunology
Meenakshi Bhardwaj, Veeresh Kumar Sali, R. Malarvizhi, Sugumar Mani, T. K. Padmavathy, Hannah Rachel Vasanthi
Summary: This study identifies a fatty acid methyl ester, methyl 6,12-dimethyltridecanoate (ET), isolated from the brown algae Turbinaria ornata, which possesses anti-inflammatory, antioxidant, and immunomodulatory potential. In vitro and in silico studies demonstrate the significant anti-inflammatory effects of ET, including the inhibition of inflammatory markers and the promotion of antioxidant enzymes. The findings establish a molecular basis for the anti-inflammatory properties of ET and its potential as a therapeutic agent.
INFLAMMOPHARMACOLOGY
(2023)
Review
Allergy
Nieves Fernandez-Gallego, Raquel Castillo-Gonzalez, Nerea Mendez-Barbero, Celia Lopez-Sanz, David Obeso, Alma Villasenor, Maria M. Escribese, Beatriz Lopez-Melgar, Jorge Salamanca, Amparo Benedicto-Buendia, Luis Jesus Jimenez-Borreguero, Borja Ibanez, Joaquin Sastre, Maria Teresa Belver, Francisco Vega, Carlos Blanco, Domingo Barber, Francisco Sanchez-Madrid, Hortensia de la Fuente, Pilar Martin, Vanesa Esteban, Rodrigo Jimenez-Saiz
Summary: Allergic diseases and atherosclerosis are two different types of immune disorders, and they have a complex clinical association and mutual influence.
Review
Biochemistry & Molecular Biology
Ju Hun Suh, Hyeon Su Joo, Eun Be Hong, Hyeon Ji Lee, Jung Min Lee
Summary: Exosomes, as an advanced therapeutic strategy for treating an overactive immune system, are gaining attention and have the potential to be applied in the treatment of various inflammatory and autoimmune diseases, showing promising prospects for the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Wendel Wohlleben, Annette Mehling, Robert Landsiedel
Summary: Chemicals can be assessed for hazard in groups based on their similar structures and toxicological properties, similar to how elements are grouped in the periodic system. Strategies for chemical grouping have been used to support the evaluation of human health hazards, exposures, and risks, as shown in case studies. Structural similarity is commonly used as the basis for grouping, but it may not always be sufficient. Ten principles for grouping have been derived, including transparency, adequacy of methods, and consideration of toxicological properties, which can facilitate effective risk management.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Medicine, Legal
Susanne N. Kolle, Melanie Flach, Marcus Kleber, David A. Basketter, Britta Wareing, Annette Mehling, Lars Hareng, Nico Watzek, Steffen Bade, Dorothee Funk-Weyer, Robert Landsiedel
Summary: In the past decade, several non-animal methods have been adopted as OECD test guidelines to identify skin sensitization hazards. However, the predictive accuracy of these methods for substances beyond the chemical domains of individual validation studies remains largely untested. This study compares the skin sensitization test results from in vitro and in chemico methods to available in vivo data for plant extracts and polymeric materials.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Rheumatology
Megan M. Hanlon, Trudy McGarry, Viviana Marzaioli, Success Amaechi, Qingxuan Song, Sunil Nagpal, Douglas J. Veale, Ursula Fearon
Summary: This study investigates the metabolic activity of infiltrating polarized macrophages in rheumatoid arthritis (RA) and their impact on pro-inflammatory responses. The researchers found that RA macrophages exhibit a unique inflammatory and metabolic phenotype, characterized by heightened expression of key cytokines and altered mitochondrial morphology. Furthermore, the study identifies NAMPT and STAT3 signaling as key regulators of this phenotype.
Article
Medicine, General & Internal
Brianne E. Barker, Megan M. Hanlon, Viviana Marzaioli, Conor M. Smith, Clare C. Cunningham, Jean M. Fletcher, Douglas J. Veale, Ursula Fearon, Mary Canavan
Summary: This study investigates the role of the mTOR pathway in the metabolism and activation of rheumatoid arthritis synovial fibroblasts (RASF), and identifies crosstalk with the Hippo-YAP pathway. The results suggest that mTOR mediates the invasive mechanism of RASF through the Hippo-YAP pathway.
FRONTIERS IN MEDICINE
(2023)
Article
Biology
M. M. Trivino, J. M. Maya-Manzano, F. Tummon, B. Clot, L. Grewling, C. Schmidt-Weber, J. Buters
Summary: During the international campaign in Munich, Germany in 2021, four manual Hirst-type pollen traps were used to compare the performance of automatic pollen traps. By correcting the pollen concentrations using a hand-held heat-wire anemometer and mathematical methods, the accuracy of the final results was improved.
Biographical-Item
Toxicology
Jan G. Hengstler, Hermann Bolt, Uwe Heinrich, Andrea Hartwig, Robert Landsiedel
ARCHIVES OF TOXICOLOGY
(2023)
Correction
Toxicology
Jan G. Hengstler, Hermann Bolt, Uwe Heinrich, Andrea Hartwig, Robert Landsiedel
ARCHIVES OF TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dunja Dimitrijevic, Eric Fabian, Dorothee Funk-Weyer, Robert Landsiedel
Summary: In pharmacokinetics, plasma protein binding (PPB) is an important parameter affecting drug metabolism. The unbound fraction (fu) is considered the effective concentration at the target site. In vitro models, such as physiologically based toxicokinetic models (PBTK), are increasingly used in pharmacology and toxicology. This study compared three methods (rapid equilibrium dialysis, ultrafiltration, and ultracentrifugation) to quantify fu and found that the selection of the separation method should be based on the properties of the test substance. Rapid equilibrium dialysis was suitable for a broader range of substances, while ultrafiltration and ultracentrifugation were suitable for polar substances.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Sabina Ramirez-Hincapie, Barbara Birk, Philipp Ternes, Varun Giri, Volker Haake, Michael Herold, Franziska Maria Zickgraf, Andreas Verlohner, Hans-Albrecht Huener, Hennicke Kamp, Peter Driemert, Robert Landsiedel, Elke Richling, Dorothee Funk-Weyer, Bennard van Ravenzwaay
Summary: Cell-based metabolomics provides multiparametric readouts for decision making in compound development. A LC-MS/MS-based metabolomics platform was developed for liver toxicity classification in HepG2 cells. The platform showed dose response and differentiation between liver toxicity modes of action, and identified specific metabolite patterns for each mode of action. This method offers a mechanistic-based, cost-effective screening for compound development pipelines.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Chemistry, Analytical
Julia Neumair, Marie Kroeger, Evamaria Stuetz, Claudia Jerin, Adam M. Chaker, Carsten B. Schmidt-Weber, Michael Seidel
Summary: Protein biomarkers in nasal secretions can be used for non-invasive diagnostics to differentiate between allergies, airway diseases, and infections. A flow-based chemiluminescence sandwich microarray immunoassay (CL-SMIA) was developed for quantification of nasal interferon-beta (IFN-β) with cost-effective immobilization of capture antibodies on polycarbonate foils. The CL-SMIA showed comparable performance and economic advantages to an enzyme-linked immunosorbent assay (ELISA) performed on microtiter plates. The small flow cell and lower consumption of detection antibodies in CL-SMIA make it a promising assay for rapid and cost-effective detection of biomarkers in nasal secretions.
Article
Toxicology
Sabina Ramirez-Hincapie, Barbara Birk, Philipp Ternes, Varun Giri, Franziska Maria Zickgraf, Volker Haake, Michael Herold, Hennicke Kamp, Peter Driemert, Robert Landsiedel, Elke Richling, Dorothee Funk-Weyer, Bennard van Ravenzwaay
Summary: This study applied a high-throughput LC-MS/MS-based broad targeted metabolomics system to investigate the metabolic dynamics of nitrofurantoin over time and concentration and derived a mechanistic-anchored approach for point of departure (PoD) derivation. The results showed clear time and concentration effects on the metabolome of HepG2 cells. This approach allows for the derivation of PoD that can be mechanistically anchored to established key events and contribute to the development of new adverse outcome pathways.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pia Wilhelmi, Varun Giri, Franziska Maria Zickgraf, Volker Haake, Stefan Henkes, Peter Driemert, Paul Michaelis, Wibke Busch, Stefan Scholz, Burkhard Flick, Marta Barenys, Barbara Birk, Hennicke Kamp, Robert Landsiedel, Dorothee Funk-Weyer
Summary: Through LC-MS/MS and GC-MS metabolomics, this study investigated the changes in endogenous metabolites in zebrafish embryos as indicators of developmental toxicity pathways. The results showed that exposure to the compound PTU led to morphological alterations and significant metabolic changes, which could be linked to the inhibition of thyroid peroxidase and potential neurodevelopmental impairments. This study demonstrated that metabolite changes in zebrafish embryos provide mechanistic information associated with the mode of action of PTU.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Medicine, Legal
Jens C. Otte, Heli Miriam Hollnagel, Christiane Nagel, Renata Friederike Gerhardt, Wendel Wohlleben, Nathalie Vallotton, Diederik Schowanek, Gordon Sanders, Joe M. Frasca, Tushar Mahale, Mark Pemberton, Bjoern Hidding, Robert Landsiedel
Summary: This study proposes a three-tiered regulatory approach for assessing polymers, which includes grouping the polymers based on their chemistry, physico-chemical properties, and hazard similarity. By reducing animal testing and providing sufficient data, this approach aims to achieve an adequate level of protection.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Toxicology
Franziska M. Zickgraf, Aishwarya Murali, Robert Landsiedel
Summary: This article summarizes studies on the effects of engineered nanomaterials (ENM) on the gut microbiome, showing that ENM can affect the microbiome in various ways, although few studies demonstrate a link to adverse effects on the host. Based on these findings, future studies can assess the hazards and risks of ENM through changes in the microbiome.
CURRENT OPINION IN TOXICOLOGY
(2023)
Correction
Environmental Sciences
Johannes G. Keller, Michael Persson, Philipp Mueller, Lan Ma-Hock, Kai Werle, Josje Arts, Robert Landsiedel, Wendel Wohlleben