期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 13, 期 4, 页码 4906-4919出版社
MDPI
DOI: 10.3390/ijms13044906
关键词
lipid nanoparticles; docetaxel; drug delivery; breast cancer
资金
- Science and Innovation Ministry [REF: SAF2009-12295, MAT2010-20370]
- Instituto de Salud Carlos III (Fondo de Investigacion Sanitaria) [P07-FQM03099]
- Consejeria de Innovacion, Ciencia y Tecnologia de la Junta de Andalucia (Spain) [P07-FQM03099, P07-FQM-2496, P10-CTS-6270]
In the field of cancer therapy, lipid nanocapsules based on a core-shell structure are promising vehicles for the delivery of hydrophobic drugs such as docetaxel. The main aim of this work was to evaluate whether docetaxel-loaded lipid nanocapsules improved the anti-tumor effect of free docetaxel in breast cancer cells. Three docetaxel-loaded lipid nanocapsules were synthesized by solvent displacement method. Cytotoxic assays were evaluated in breast carcinoma (MCF-7) cells treated by the sulforhodamine B colorimetric method. Cell cycle was studied by flow cytometry and Annexin V-FITC, and apoptosis was evaluated by using propidium iodide assays. The anti-proliferative effect of docetaxel appeared much earlier when the drug was encapsulated in lipid nanoparticles than when it was free. Docetaxel-loaded lipid nanocapsules significantly enhanced the decrease in IC50 rate, and the treated cells evidenced apoptosis and a premature progression of the cell cycle from G(1) to G(2)-M phase. The chemotherapeutic effect of free docetaxel on breast cancer cells is improved by its encapsulation in lipid nanocapsules. This approach has the potential to overcome some major limitations of conventional chemotherapy and may be a promising strategy for future applications in breast cancer therapy.
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