期刊
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
卷 70, 期 3, 页码 267-284出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09637486.2018.1505834
关键词
Yerba mate; green coffee; microbial metabolites; EA.hy926 cells; TNF-alpha; caffeoylquinic acid
资金
- Spanish Ministry of Economy and Competitivity [AGL2010-18269, AGL2015-69986-R]
- Consejo Superior de Investigaciones Cientificas (CSIC) [PIE-201670E061]
- Chinese Government
The main phenol in mate and coffee, 5-caffeoylquinic-acid (5-CQA), and its relevant microbial metabolites, dihydrocaffeic (DHCA) and dihydroferulic (DHFA) acids, have shown oxidative-stress protective effects in HepG2 cells. To evaluate possible endothelial-protective effects of the extracts and compounds, endothelial EA.hy926 cells were pre-treated with yerba mate (YME) and green coffee bean (GCBE) phenolic extracts, 5-CQA, DHCA and DHFA and afterwards stressed with tumour-necrosis-factor-alpha (TNF-alpha). Then oxidative-stress markers and endothelial-nitric-oxide-synthase levels were studied. TNF-alpha (10 ng/mL, 24 h) depleted reduced glutathione (GSH) and eNOS levels, increased reactive oxygen species (ROS) production, glutathione peroxidase (GPx) and reductase (GR) activities, and protein oxidation (carbonyl groups, CG) in EA.hy926 cells. Pre-treatment with YME, GCBE, 5-CQA, DHCA at certain physiological concentrations, lowered ROS production, recovered depleted GSH, reduced GR and GPx activities, and CG levels, and enhanced eNOS concentration.. YME, GCBE and 5-CQA show antioxidant effects in endothelial cells playing DHCA an important role in such protection; moreover, the extracts, 5-CQA, DHCA and DHFA increased eNOS levels.
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