期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 178, 期 -, 页码 60-64出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2014.03.005
关键词
Torque teno sus virus; Pork chops; Pork livers; Retail meat
资金
- Agriculture and Agri-Food Canada Research Branch Project (RBPI) [1485]
Torque teno viruses (ITV) are widespread in humans, swine as well as in several other animal species. In market ready swine, the reported prevalence ranges between 11% and 100%. Through a national retail sampling plan from the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) program, 283 and 599 liver and pork chop samples, respectively, were collected over a 12-month period from commercial establishments in 5 selected geographical regions of Canada to assess the presence of Torque teno sus viruses (ITSuVs) in these products. TTSuVs were detected in 97.9% of pork chops with viral loads ranging between I x 10(4) and 9.9 x 10(5) genomic copies (gc)/g and 98.6% of liver samples with viral loads ranging from 1 x 10(5) to 9.9 x 10(6) gc/g. A selection of 20 positive samples (10 pork chop and 10 liver) from the 5 geographical regions were further tested for the production, of a 305 bp fragment for TTSuV1 and a 253 bp fragment for TTSuV2 in the non-coding region. TTSuV1 was present in all 10 liver and 10 pork chops samples while TTSuV2 was detected in 10 liver and 9 pork chop samples. Two different 1TSuV1 sequences were simultaneously detected from 5 of 20 samples and 2 different 1TSuV2 sequences were detected from 6 of 19 samples. The omnipresence of TTSuVs in commercial pork samples may allow its use as a viral indicator to monitor the effectiveness of cleaning and disinfecting process in slaughtering, cutting, slicing and packaging facilities. Crown Copyright (C) 2014 Published by Elsevier B.V. All rights reserved.
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