期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 170, 期 -, 页码 65-69出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2013.11.002
关键词
Enterococci; Virulence gene; Biofilm formation; Suboptimal temperature
资金
- Natural Sciences and Engineering Research Council of Canada
Twenty-nine Enterococcus strains from raw and fermented meat products were screened for the presence of virulence genes, including those for aggregation substances (asa1 and asa373), cytolysin activator (cylA), collagen binding protein (ace), endocarditis antigen (efaA), enterococcal surface protein (esp) and gelatinase (gelE). Virulence gene occurrence, expression of gelatinase and pheromone aggregation was greater in Enterococcus faecalis than in Entero coccus faecium strains. All E. faecalis and 54% of E. faecium were positive for at least one or more virulence gene. The only strain of Enterococcus gallinarum tested also contained virulence genes. The effect of different growth temperatures (25 and 37 degrees C) on biofilm formation using polystyrene plates was also assessed. Strong biofilm formation occurred at lower than optimum temperature in all three species of enterococci. Neither esp nor gelE was necessary for biofilm formation and this relationship was species rather than strain specific. This study emphasizes the importance of enterococci as a reservoir of virulence genes and the potential for their genetic transfer to human strains following consumption of uncooked or undercooked contaminated meat. (C) 2013 Elsevier B.V. All rights reserved.
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