期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 185, 期 -, 页码 121-126出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2014.06.001
关键词
Bacteriocins; Virulence; Antibiotic resistance; Biogenic amines; Lactic acid bacteria; Goat milk
资金
- CAPES
- CNPq
- FAPESP
- FAPEMIG
The present study aimed to investigate the virulence, antibiotic resistance and biogenic amine production in bacteriocinogenic lactococci and enterococci isolated from goat milk in order to evaluate their safety. Twenty-nine bacteriocinogenic lactic acid bacteria (LAB: 11 Lactococcus spp., and 18 Enterococcus spp.) isolated from raw goat milk were selected and subjected to PCR to identify gelE, cylA, hyl, asa1, esp, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc genes. The expression of virulence factors (gelatinase, hemolysis, lipase, DNAse, tyramine, histamine, putrescine) in different incubation temperatures was assessed by phenotypic methods, as well as the resistance to vancomycin, gentamicin, chloramphenicol, ampicillin and rifampicin (using Eteste (R)). The tested isolates presented distinct combinations of virulence related genes, but not necessarily the expression of such factors. The relevance of identifying virulence-related genes in bacteriocinogenic LAB was highlighted, demanding for care in their usage as starter cultures or biopreservatives due to the possibility of horizontal gene transfer to other bacteria in food systems. (C) 2014 Elsevier B.V. All rights reserved.
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