期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 149, 期 2, 页码 177-182出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2011.06.012
关键词
AFLP; PFGE; Listeria monocytogenes subtyping
Listeria monocytogenes ranks among the most frequent causes of death due to foodborne illness (20-30% case fatality rate). Discriminative subtyping methods are important to detect the relatedness of isolates and verify epidemiologic associations. AFLP analysis is a DNA fingerprinting technique based on the selective amplification of genomic restriction fragments. In this study, two AFLP methods and PFGE were compared in regard to discriminatory power, typeability and concordance. A total of 103 unrelated L monocytogenes strains isolated from different environmental and food sources were analyzed. Strains were isolated from samples obtained from food-production plants, supermarkets and small food markets in Piedmont, Italy. All methods clustered L monocytogenes strains into two genetic lineages. Lineage I and II. The three methods were compared using the 82 isolates which were typeable with all techniques. The calculated pair-wise Pearson's correlation coefficients (r) showed close agreement between all three methods. Our findings suggest that the AFLP II method can be successfully used to subtype L monocytogenes strains isolated from foods and food processing facilities. (C) 2011 Elsevier BM. All rights reserved.
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