期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 142, 期 1-2, 页码 106-113出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2010.06.009
关键词
Model; Validation; Growth; Botrytis cinerea; Penicillium expansum; Mould
资金
- National Program on earthy/musty taste of wine (AGRIMER, Paris, France) [01 04 21 08 0004 21]
The objective of this study was to develop and to validate a model for predicting the combined effect of temperature and a(w) on the radial growth rate, mu, of Botrytis cinerea and Penicillium expansum on grape berries. The proposed strategy was based on the gamma-concept developed previously [Zwietering, Wijtzes, T., de Wit, J.C., van't Riet, K. 1992. A decision support system for prediction of the microbial spoilage in foods. Journal of Food Protection. 12, 973-979]: mu=mu(opt).gamma(T).gamma(a(w)), where the gamma functions were cardinal models with inflection (CMI), mu(opt) the radial growth rate on grape berries. Firstly, the cardinal temperatures and a(w)'s were estimated independently from experiments carried out on Potato Dextrose Agar. Secondly, the gamma concept was validated if on a synthetic grape juice medium (SGJ) and ii/ on a grape juice agar (GJA). Accuracy and bias factors were closer to 1 with the latter analogue, thus suggesting that GJA should be preferred to SGJ. Thirdly, an experimental protocol taken into account the isotropic nature of fungal growth was developed for estimating mu(opt) on grape berries. This study demonstrated that CMI's can be validated on agri-food products over a wide range of temperature and a(w) using the described methodology. (C) 2010 Elsevier B.V. All rights reserved.
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