期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 144, 期 1, 页码 187-192出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2010.09.009
关键词
Saccharomyces cerevisiae; Indigenous strains; ITS-RFLP; Fermentation by-products; mtDNA-RFLP profile
资金
- Regional Government of Sicily (Italy) - Assessorato Agricoltura e Foreste [194]
The present research studied Saccharomyces cerevisiae yeasts isolated from Nero d'Avola grapes, collected in different areas of the Sicily region. RAPD-PCR analysis with M13 primer was used for preliminary discrimination among 341 S. cerevisiae isolates. Inoculated fermentations with S. cerevisiae strains, exhibiting different RAPD-PCR fingerprinting, revealed the impact of selected strains on volatile compound concentration. Two selected strains were used in fermentation at cellar level and the restriction analysis of mtDNA on yeast colonies isolated during fermentation was used to control strain implantation. This study represents an important step to establish a collection of indigenous S. cerevisiae strains isolated from a unique environment, such as Nero d'Avola vineyards. Different starter implantation throughout inoculated fermentation represents an additional character, which might be considered during the selection program for wine starter cultures. (C) 2010 Elsevier B.V. All rights reserved.
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