Article
Biotechnology & Applied Microbiology
Katarina Butorac, Jasna Novak, Barbara Bellich, Lucrecia C. Teran, Martina Banic, Andreja Lebos Pavunc, Slaven Zjalic, Paola Cescutti, Jagoda Suskovic, Blazenka Kos
Summary: Lactobacillus fermentum D12 is a strain capable of producing exopolysaccharides (EPS) with potential health benefits. Its whole genome analysis revealed key genes related to EPS biosynthesis, leading to the secretion of multiple EPSs under different culture conditions. EPS positively influences the survival and adhesion of D12, especially under simulated gastrointestinal tract conditions.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Wenping Lei, Qi Chen, Yan Liu, Xia Tang, Jie Luo, Chengguo Liu, Hui Zhou
Summary: Exopolysaccharides (EPS) secreted from lactic acid bacteria (LAB) have health-promoting properties and contribute to the rheology of fermentation milk products. A newly discovered EPS, EPS-NS1905E, has higher viscosity and strong antioxidant abilities.
JOURNAL OF FOOD BIOCHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Xingtao Zhou, Duoduo Zhang, Wucheng Qi, Tao Hong, Tao Xiong, Tingqin Wu, Fang Geng, Mingyong Xie, Shaoping Nie
Summary: EPS116 promotes intestinal epithelial regeneration by increasing the number of intestinal stem cells and modulating the gut microbiota profile, thereby maintaining intestinal barrier and homeostasis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Xuefang Zhao, Qi Liang
Summary: This study evaluated the effects of probiotic Lactobacillus plantarum MC5 on the quality, antioxidant activity, and storage stability of yogurt. The results showed that adding MC5 can improve the rheological properties, texture, and antioxidant activity of yogurt. The study also found that MC5 can promote the growth of Streptococcus thermophilus. Therefore, L. plantarum MC5 has potential for industrial application in milk fermentation.
Article
Agriculture, Dairy & Animal Science
Yunyun Jiang, Min Zhang, Yang Zhang, Justyna Zulewska, Zhennai Yang
Summary: This study investigated the impact of Ca2+ on EPS biosynthesis in the probiotic Lactobacillus plantarum K25. Results showed that Ca2+ increased EPS yield, changed monosaccharide composition, upregulated proteins related to EPS synthesis, and influenced small molecule metabolites related to EPS biosynthesis pathways. The activation of specific signaling pathways by Ca2+ provided insights into the regulation of EPS synthesis genes in L. plantarum K25.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Veterinary Sciences
Shilei Huang, Qiuhan Yu, Luyi Xie, Ling Ran, Kai Wang, Yang Yang, Lu Gan, Zhenhui Song
Summary: The study showed that Lactobacillus plantarum metabolites and exopolysaccharides have inhibitory effects on Porcine Epidemic Diarrhea Virus replication, with exopolysaccharides exhibiting strong antiviral properties. Exopolysaccharides can prevent virus adsorption and induce early apoptosis of infected cells.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Xuefang Zhao, Qi Liang
Summary: This study optimized the production of exopolysaccharides (EPS) from Lactiplantibacillus plantarum MC5 (Lp. plantarum MC5), evaluated its resistance to digestive juices, antioxidant activity, and rheological properties. The results showed that the maximum EPS production was achieved under specific conditions. The EPS-MC5 exhibited high resistance to digestive enzymes and antioxidant activity, as well as favorable rheological properties. These findings contribute to the understanding and potential applications of EPS in human health and functional foods.
Article
Food Science & Technology
Ting Ding, Yong Li
Summary: Walnut oligopeptides (WOPs) as prebiotics can promote the growth and resistance ability of Lactobacillus plantarum Z7 by enhancing bacterial biofilm secretion and promoting the ability of bacteria to resist adverse environments.
FOOD SCIENCE & NUTRITION
(2021)
Article
Biotechnology & Applied Microbiology
Kun-tai Li, Shuai-ying Peng, Bao Zhang, Wei-fu Peng, Shui-jing Yu, Xin Cheng
Summary: Exopolysaccharides (EPSs) can alleviate the toxic effect of cadmium (Cd) on rice and reduce Cd accumulation in grains. EPSs can facilitate the accumulation of Cd in rice roots and reduce Cd transportation from roots to leaves. Additionally, EPSs can affect the subcellular distribution and chemical forms of Cd in different parts of rice seedlings.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Cell Biology
Qingqing Zhang, Qing Zhao, Tao Li, Longya Lu, Fei Wang, Hong Zhang, Zhi Liu, Huihui Ma, Qihui Zhu, Jingjing Wang, Xuemei Zhang, Yang Pei, Qisha Liu, Yuyu Xu, Jinlong Qie, Xiaoting Luan, Zhibin Hu, Xingyin Liu
Summary: Previous studies have shown that probiotics can ameliorate colorectal cancer in a mouse model, but the underlying mechanisms are still unclear. In this study, the researchers found that a specific probiotic strain and its metabolite could improve intestinal inflammation, tumor growth, and gut dysbiosis. Further investigation revealed that the metabolite enhanced the production of IL12a in dendritic cells, priming CD8+ T cell immunity against tumor growth, and also regulated cholesterol metabolism in CD8+ T cells. These findings provide new insights into the epigenetic regulation of probiotics-mediated anti-tumor immunity and suggest potential therapeutic strategies for colorectal cancer patients.
Article
Agricultural Engineering
M. P. Soumya, Reeba Parameswaran, K. Madhavan Nampoothiri
Summary: Glycosyltransferases synthesize exopolysaccharides (EPS) by modifying the properties of the polymers, such as glycosidic linkage, branching, length, mass, and conformation. A study on Lactobacillus plantarum BR2 (Accession No: MN176402) identified twelve glycosyltransferase genes, including BR2gtf (1116 bp), which was cloned into the pNZ8148 vector as an EPS biosynthetic glycosyltransferase. The over-expression of gtf gene in L. plantarum BR2 using a nisin-controlled expression system resulted in a 54.4% increase in EPS production, reaching a maximum of 23.2 ± 0.5 g/L in a 5 L bioreactor study after 72 h of fermentation. This study demonstrates a potential molecular strategy to enhance exopolysaccharide production in lactic acid bacteria.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mibang Kim, Dong-Hyun Jung, Dong-Ho Seo, Young-Seo Park, Myung-Ji Seo
Summary: Carotenoids, including 4,4'-diaponeurosporene, are natural pigments with beneficial effects, widely used in various industries. The antioxidant activity of 4,4'-diaponeurosporene extracted from L. plantarum subsp. plantarum KCCP11226 was found to be higher than that of a common antioxidant food additive, demonstrating its potential for functional probiotic industries.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Renato Kovacs, Lorand Erdelyi, Ferenc Fenyvesi, Noemi Balla, Fruzsina Kovacs, Gyoergy Vamosi, Agnes Klusoczki, Alexandra Gyoengyoesi, Ildiko Bacskay, Miklos Vecsernyes, Judit Varadi
Summary: The antimicrobial effect of chitosan and its derivatives has been confirmed on various bacteria and fungi. The tests conducted on pathogenic microorganisms revealed the inhibitory effect mechanism and concentration dependence of chitosan. By adding commercially available chitosan derivatives to a probiotic strain, it was determined that chitosan has a viabilty decreasing effect at concentrations above the minimum inhibitory concentration (MIC). The study also identified increased cell membrane permeability and irreversible interaction between low molecular weight chitosan and bacterial cells.
Article
Biology
Thiruvengadam Shankar, Selvam Palpperumal, Durairaj Kathiresan, Subbiah Sankaralingam, Chellapandian Balachandran, Kathirvelu Baskar, Abeer Hashem, Abdulaziz A. Alqarawi, Elsayed Fathi Abd-Allah
Summary: The study evaluated the purification and characterization of exopolysaccharides from Lactobacillus paracasei, showing potential antioxidant properties.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
M. P. Soumya, K. Madhavan Nampoothiri
Summary: This study focuses on the production and chemical modifications of EPS produced by Lactobacillus plantarum BR2, and the evaluation of their biological properties. The results show that the modified derivatives have better antioxidant properties compared to the unmodified form. Among the derivatives, acetylated EPS (a-EPS) exhibits the highest DPPH radical scavenging and total antioxidant activity, while sulphated EPS (s-EPS) shows the highest reducing power potential and hydroxyl radical scavenging activities. The improved antioxidant properties open up better avenues for EPS application in the food and pharma sectors.
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)