Article
Oncology
Adan Pinto-Fernandez, Mariolina Salio, Tom Partridge, Jianzhou Chen, George Vere, Helene Greenwood, Cyriel Sebastiaan Olie, Andreas Damianou, Hannah Claire Scott, Henry Jack Pegg, Alessandra Chiarenza, Laura Diaz-Saez, Paul Smith, Claudia Gonzalez-Lopez, Bhavisha Patel, Emma Anderton, Neil Jones, Tim R. Hammonds, Kilian Huber, Ruth Muschel, Persephone Borrow, Vincenzo Cerundolo, Benedikt M. Kessler
Summary: This study has identified novel ISGylation targets in chronic myeloid leukaemia (CML)-derived cell line, shedding light on the potential of USP18 as a cancer target for regulating antigenicity and radiosensitivity. The findings suggest that USP18 plays a crucial role in modulating immune responses and sensitivity to treatment in cancer cells.
BRITISH JOURNAL OF CANCER
(2021)
Article
Multidisciplinary Sciences
Jinxiu Hou, Lulu Han, Ze Zhao, Huiqing Liu, Lei Zhang, Chunhong Ma, Fan Yi, Bingyu Liu, Yi Zheng, Chengjiang Gao
Summary: Research demonstrates that deubiquitinase USP18 regulates MAVS-mediated antiviral signaling by promoting MAVS interaction with TRIM31, leading to increased production of type I interferon and enhanced resistance to RNA viruses.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Kei-ichiro Arimoto, Sayuri Miyauchi, Ty D. Troutman, Yue Zhang, Mengdan Liu, Samuel A. Stoner, Amanda G. Davis, Jun-Bao Fan, Yi-Jou Huang, Ming Yan, Christopher K. Glass, Dong-Er Zhang
Summary: Immunotherapy is an effective cancer treatment, but not for all patients, prompting the need for alternative strategies. Inducing cancer immunogenic cell death (ICD) shows promise in promoting robust immune responses against tumor-associated antigens. Depletion of USP18, a negative regulator of interferon signaling, selectively induces ICD in cancer cells, suggesting targeting USP18 as a potential cancer immunotherapy.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Cyriel Sebastiaan Olie, Adan Pinto-Fernandez, Andreas Damianou, Iolanda Vendrell, Hailiang Mei, Bianca den Hamer, Erik van der Wal, Jessica C. de Greef, Vered Raz, Benedikt M. Kessler
Summary: The ubiquitin proteasomal system plays a critical role in muscle physiology, but the function of deubiquitinating enzymes (DUBs) in muscle cells remains poorly understood. Through a genetic screen, we discovered that the depletion of USP18, a DUB, affects muscle cell differentiation. USP18 regulates differentiation initiation and maintenance independently of ISG15 and the ISG response. Alterations in gene networks and protein expression profiles were observed in the absence of USP18, leading to disrupted calcium homeostasis and reduced contractile force.
CELL DEATH & DISEASE
(2023)
Article
Immunology
Marta Martin-Fernandez, Sofija Buta, Tom Le Voyer, Zhi Li, Lasse Toftdal Dynesen, Francoise Vuillier, Lina Franklin, Fatima Ailal, Alice Muglia Amancio, Louise Malle, Conor Gruber, Ibtihal Benhsaien, Jennie Altman, Justin Taft, Caroline Deswarte, Manon Roynard, Alejandro Nieto-Patlan, Kunihiko Moriya, Jeremie Rosain, Nathalie Boddaert, Aziz Bousfiha, Yanick J. Crow, Dragana Jankovic, Alan Sher, Jean-Laurent Casanova, Sandra Pellegrini, Jacinta Bustamante, Dusan Bogunovic
Summary: This study identifies a new USP18 mutation that leads to autoinflammation and mycobacterial disease due to insufficient negative regulation of IFN-I signaling, providing important insights into the underlying mechanism of specific interferonopathy.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Review
Virology
Nicholas A. Mathieu, Ermela Paparisto, Stephen D. Barr, Donald E. Spratt
Summary: ISG15 is a crucial immunoprotein that plays a significant role in antiviral immunity, with HERC5 regulating its signaling pathway to promote the ISGylation of viral proteins in response to various viral infections.
Article
Microbiology
Haiyan Ye, Xiaoqiong Duan, Min Yao, Lan Kang, Yujia Li, Shilin Li, Bin Li, Limin Chen
Summary: The research found that DENV infection can increase the expression of USP18, which in turn promotes the replication of DENV-2. Silencing USP18 can enhance the resistance of IFN-alpha to DENV-2.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anne Clancy, Emma V. Rusilowicz-Jones, Iona Wallace, Kirby N. Swatek, Sylvie Urbe, Michael J. Clague
Summary: Type 1 interferon stimulation leads to up-regulation of a ubiquitin-like conjugation system. USP18, a member of the deubiquitylase family, is up-regulated and can inhibit JAK-STAT signaling. USP18's role in cell viability and growth inhibition is not solely dependent on ISGylation activity.
BIOCHEMICAL JOURNAL
(2023)
Article
Oncology
Xi Liu, Yun Lu, Zibo Chen, Xiuxia Liu, Weiguo Hu, Lin Zheng, Yulong Chen, Jonathan M. Kurie, Mi Shi, Lisa Maria Mustachio, Thorkell Adresson, Stephen Fox, Jason Roszik, Masanori Kawakami, Sarah J. Freemantle, Ethan Dmitrovsky
Summary: The study shows that USP18 plays a crucial role in regulating lipid and fatty acid metabolism, potentially affecting proliferation of lung cancer cells by modulating the expression of ATGL and UCP1. Moreover, the combined expression of USP18, ATGL, and UCP1 in lung cancer patients is associated with unfavorable survival outcomes.
MOLECULAR CANCER RESEARCH
(2021)
Article
Immunology
Alice Burleigh, Elena Moraitis, Eman Al Masroori, Eslam Al-Abadi, Ying Hong, Ebun Omoyinmi, Hannah Titheradge, Karen Stals, Wendy D. Jones, Anthony Gait, Vignesh Jayarajan, Wei-Li Di, Neil Sebire, Lea Solman, Malobi Ogboli, Steven B. Welch, Annapurna Sudarsanam, Ian Wacogne, Fiona Price-Kuehne, Barbara Jensen, Paul A. Brogan, Despina Eleftheriou
Summary: ISG15 deficiency is a rare disease caused by variants in the ISG15 gene, resulting in various clinical phenotypes. This report describes novel variants found in two families and demonstrates the effectiveness of the JAK inhibitor baricitinib in resolving clinical symptoms associated with ISG15 deficiency.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Fabien Thery, Denzel Eggermont, Francis Impens
Summary: ISG15 is an important antimicrobial protein that acts through the ISGylation process, but its substrate repertoire remains unclear. Mass spectrometry-based proteomics studies can reveal the mechanism of action and substrates of ISG15.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Dhiraj Singh, Ekaterina Aladyeva, Shibali Das, Bindu Singh, Ekaterina Esaulova, Amanda Swain, Mushtaq Ahmed, Journey Cole, Chivonne Moodley, Smriti Mehra, Larry Schlesinger, Maxim Artyomov, Shabaana Khader, Deepak Kaushal
Summary: Singh et al. conducted longitudinal scRNA-Seq on rhesus macaques infected with SARS-CoV-2 to gain insights into early immune dynamics. They identified a distinct population of macrophages with an interferon-driven anti-viral gene signature, which correlated with viral clearance.
NATURE COMMUNICATIONS
(2022)
Review
Virology
Cindy Chiang, Guanqun Liu, Michaela U. Gack
Summary: Viral manipulation of ubiquitin or ISG15-mediated immune responses is a key strategy for virus evasion, including direct targeting of ubiquitin E3 ligases, active deubiquitination using viral DUBs, and the upregulation of cellular DUBs to regulate RLR signaling. Certain coronaviruses, such as SARS-CoV-2, actively deISGylate key molecules in the RLR pathway to escape type I interferon-mediated antiviral responses. Targeting virally-encoded proteins that manipulate ubiquitin or ISG15-mediated innate immune responses may be a potential strategy for the development of new antivirals and vaccines.
Article
Reproductive Biology
Xinyan Zhang, Suhua Gong, Haijing Li, Jiaqi Jiang, Yanni Jia, Ruixue Zhang, Haokun Liu, Aihua Wang, Yaping Jin, Pengfei Lin
Summary: In this study, the role and mechanism of Ubiquitin-specific protease 18 (USP18) on endometrial receptivity in goats were investigated. The results showed that USP18 promotes endometrial receptivity by regulating the JAK/STAT1 pathway and ISGylation. Additionally, the expression levels of USP18 were significantly increased during early pregnancy. Therefore, USP18 may play an important role in goat endometrial receptivity.
Article
Biochemistry & Molecular Biology
Gaelan Melanson, Antonia C. Du Bois, Caroline Webster, James Uniacke
Summary: In hypoxia and interferon-induced cells, ISG15 and HIFs regulate each other, altering HIF transcriptional programmes and increasing the translation activity of HIF-2alpha.
Article
Surgery
M. Knaak, N. Goldaracena, A. Doyle, M. S. Cattral, P. D. Greig, L. Lilly, I. D. McGilvray, G. A. Levy, A. Ghanekar, E. L. Renner, D. R. Grant, M. Selzner, N. Selzner
AMERICAN JOURNAL OF TRANSPLANTATION
(2017)
Review
Transplantation
Agata Bartczak, Ian McGilvray, Armand Keating
CURRENT OPINION IN ORGAN TRANSPLANTATION
(2017)
Article
Endocrinology & Metabolism
Kejing Zeng, Lili Tian, Rucha Patel, Weijuan Shao, Zhuolun Song, Ling Liu, Justin Manuel, Xuezhong Ma, Ian McGilvray, Carolyn L. Cummins, Jianping Weng, Tianru Jin
Article
Oncology
Adriana Fonseca, Abha Gupta, Furqan Shaikh, Raveena Ramphal, Vicky Ng, Ian McGilvray, J. Ted Gerstle
PEDIATRIC BLOOD & CANCER
(2018)
Article
Surgery
Aloysious D. Aravinthan, Andrew S. Barbas, Adam C. Doyle, Mahmood Tazari, Gonzalo Sapisochin, Mark S. Cattral, Anand Ghanekar, Ian D. McGilvray, Markus Selzner, Paul D. Greig, Mamatha Bhat, Nazia Selzner, David R. Grant, Leslie B. Lilly, Eberhard L. Renner
TRANSPLANT INTERNATIONAL
(2017)
Article
Virology
Robert A. Kozak, Larissa Hattin, Mia J. Biondi, Juan C. Corredor, Scott Walsh, Max Xue-Zhong, Justin Manuel, Ian D. McGilvray, Jason Morgenstern, Evan Lusty, Vera Cherepanov, Betty-Anne McBey, David Leishman, Jordan J. Feld, Byram Bridle, Eva Nagy
Article
Radiology, Nuclear Medicine & Medical Imaging
Kartik Jhaveri, Lijun Guo, Luis Guimares, Ravi Menezes, Ian McGilvray, Mark Cattral, David Grant
ABDOMINAL RADIOLOGY
(2018)
Article
Oncology
Hala Muaddi, David P. Al-Adra, Rob Beecroft, Anand Ghanekar, Carol-Anne Moulton, Adam Doyle, Markus Selzner, Alice Wei, Ian D. McGilvray, Steven Gallinger, David R. Grant, Mark S. Cattral, Paul D. Greig, John Kachura, Sean P. Cleary, Gonzalo Sapisochin
ANNALS OF SURGICAL ONCOLOGY
(2018)
Article
Cell Biology
Yujia Li, MaX Xuezhong Ma, Bo Qin, Liang-Tzung Lin, Christopher D. Richardson, Jordan Feld, Ian D. McGilvray, Limin Chen
MEDIATORS OF INFLAMMATION
(2019)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jia Qu, Albert Fung, Paul Kelly, Gordon Tait, Paul D. Greig, Anne Agur, Ian D. McGilvray, Jodie Jenkinson
JOURNAL OF VISUAL COMMUNICATION IN MEDICINE
(2017)
Article
Transplantation
Juan Echeverri, Nicolas Goldaracena, Akhil Kant Singh, Gonzalo Sapisochin, Nazia Selzner, Mark S. Cattral, Paul D. Greig, Les Lilly, Ian D. McGilvray, Gary A. Levy, Anand Ghanekar, Eberhard L. Renner, David R. Grant, Stuart A. McCluskey, Markus Selzner
TRANSPLANTATION DIRECT
(2017)
Article
Transplantation
Andrew S. Barbas, David P. Al-Adra, Nicolas Goldaracena, Martin J. Dib, Markus Selzner, Gonzalo Sapisochin, Mark S. Cattral, Ian D. McGilvray
TRANSPLANTATION DIRECT
(2017)
Article
Oncology
Aloysious D. Aravinthan, Silvio G. Bruni, Adam C. Doyle, Hla-Hla Thein, Nicolas Goldaracena, Assaf Issachar, Leslie B. Lilly, Nazia Selzner, Mamatha Bhat, Boraiah Sreeharsha, Markus Selzner, Anand Ghanekar, Mark S. Cattral, Ian D. McGilvray, Paul D. Greig, Eberhard L. Renner, David R. Grant, Gonzalo Sapisochin
ANNALS OF SURGICAL ONCOLOGY
(2017)
Article
Transplantation
Andrew S. Barbas, Nicolas Goldaracena, Martin J. Dib, David P. Al-Adra, Aloysious D. Aravinthan, Leslie B. Lilly, Eberhard L. Renner, Nazia Selzner, Mamatha Bhat, Mark S. Cattral, Anand Ghanekar, Ian D. McGilvray, Gonzalo Sapisochin, Markus Selzner, Paul D. Greig, David R. Grant
TRANSPLANTATION DIRECT
(2017)
Article
Chemistry, Multidisciplinary
Sonya A. MacParland, Kim M. Tsoi, Ben Ouyang, Xue-Zhong Ma, Justin Manuel, Ali Fawaz, Mario A. Ostrowski, Benjamin A. Alman, Anton Zilman, Warren C. W. Chan, Ian D. McGilvray
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)