Article
Infectious Diseases
Taehyeong Lim, So-Young Ham, SangJin Nam, Myoungsun Kim, Ki Yong Lee, Hee-Deung Park, Youngjoo Byun
Summary: This article reviews recent studies on small molecules targeting the RhlI/RhlR system in P. aeruginosa, discussing their in vitro and in vivo biological activities. The article focuses on structure-activity relationship studies and the outlook for next-generation anti-biofilm agents.
Article
Biotechnology & Applied Microbiology
James Redfern, Janine Wallace, Alex van Belkum, Magali Jaillard, Elliot Whittard, Roobinidevi Ragupathy, Joanna Verran, Peter Kelly, Mark Charles Enright
Summary: Pseudomonas aeruginosa is a common environmental microorganism that can form biofilms on indwelling device surfaces, promoting adhesion and survival. Research identified accessory genes and core-genome SNPs associated with enhanced biofilm production at 22 degrees C, including a 165 kb genomic island with heavy metal resistance genes.
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Biology
Steffen Geisel, Eleonora Secchi, Jan Vermant
Summary: Biofilms are bacterial communities encased by a self-produced matrix, and the channel networks formed within the biofilm play a vital role in promoting the transport of nutrients and chemicals. This study focuses on the role of the biofilm matrix adhesion in channel formation, specifically in Pseudomonas aeruginosa biofilms grown under constant flow in microfluidic channels. The formation of wrinkles and folds is triggered by a mechanical buckling instability, resulting in hollow channels that facilitate bacterial movement.
Review
Biotechnology & Applied Microbiology
Anming Xu, Xiaoxiao Zhang, Tong Wang, Fengxue Xin, Luyan Z. Ma, Jie Zhou, Weiliang Dong, Min Jiang
Summary: Pseudomonas aeruginosa is known for its excellent ecological flexibility and can survive in diverse niches. The adaptive mutants RSCVs have been frequently isolated from chronic infections, sparking interest in investigating their formation, persistence, and pathogenesis. RSCVs are believed to play a significant role in biofilm formation and high adaptability.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Xiaodie Yuan, Xia Zhao, Yuan Lin, Zhaohui Su
Summary: This study synthesized PDA nanoparticles of various sizes and introduced different surface groups to investigate their penetration and accumulation abilities in biofilms. The PDA nanoparticles of approximately 60 nm size showed higher penetration and accumulation abilities, and the positively charged amine groups were more effective in enhancing the interactions with the biofilm compared to carboxyl or methoxy groups. The PDA60 nanoparticles with amine surface groups also exhibited good photothermal properties.
Article
Infectious Diseases
Emilie Charpentier, Ludovic Doudet, Ingrid Allart-Simon, Marius Colin, Sophie C. Gangloff, Stephane Gerard, Fany Reffuveille
Summary: The combination of non-antimicrobial molecules with antibiotics can significantly reduce bacterial biofilm formation, potentially offering a solution to combat antibiotic resistance emergence.
Article
Pharmacology & Pharmacy
Davoodbasha MubarakAli, Kannappan Arunachalam, Murugan Lakshmanan, Bazigha Badar, Jung-Wan Kim, Sang-Yul Lee
Summary: Biofilm-related nosocomial infections can cause various life-threatening infections. In this study, hydroxyapatite nanoparticles (HANPs) were synthesized using a co-precipitation method followed by microwave treatment, and their ability to inhibit the biofilm formation of Pseudomonas aeruginosa ATCC 10145 was investigated. The results showed that the synthesized HANPs effectively inhibited biofilm formation, suggesting their potential use in controlling biofilm-related infections caused by Pseudomonas aeruginosa.
Article
Immunology
Zuoji Ye, Liumei Ye, Dingbin Li, Shunsheng Lin, Wusheng Deng, Li Zhang, Jinhua Liang, Jinlong Li, Qingjun Wei, Ke Wang
Summary: In this study, the researchers found that Daphnetin (DAP) can effectively inhibit and eradicate biofilm formation of Pseudomonas aeruginosa. DAP also reduced bacterial motility and pyocyanin production. These findings suggest that DAP may serve as a natural anti-biofilm therapeutic agent.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Christian Schuetz, Duy-Khiet Ho, Mostafa Mohamed Hamed, Ahmed Saad Abdelsamie, Teresa Roehrig, Christian Herr, Andreas Martin Kany, Katharina Rox, Stefan Schmelz, Lorenz Siebenbuerger, Marius Wirth, Carsten Boerger, Samir Yahiaoui, Robert Bals, Andrea Scrima, Wulf Blankenfeldt, Justus Constantin Horstmann, Rebekka Christmann, Xabier Murgia, Marcus Koch, Aylin Berwanger, Brigitta Loretz, Anna Katharina Herta Hirsch, Rolf Wolfgang Hartmann, Claus-Michael Lehr, Martin Empting
Summary: A new generation of quorum sensing inhibitors (QSI) is reported to effectively inhibit the virulence of Pseudomonas aeruginosa and enhance antibiotic efficacy, potentially preventing the development of antimicrobial resistance. Additionally, a significant synergistic effect of QSI in combination with tobramycin against P. aeruginosa biofilms is demonstrated, showing promising implications for adjunctive pathoblocker-mediated therapy.
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Immunology
Qi Tan, Qing Ai, Yu He, Fang Li, Jialin Yu
Summary: The ability of P.aeruginosa biofilms to activate inflammasomes and increase inflammatory response contributes to chronic infection. This study found that P.aeruginosa biofilms significantly enhance the production of IL-1β and IL-18, possibly through the regulation of NLRP3 inflammasomes rather than NLRC4 inflammasomes.
MICROBIAL PATHOGENESIS
(2022)
Article
Immunology
Laia Fernandez-Barat, Nil Vazquez Burgos, Victoria Alcaraz, Leticia Bueno-Freire, Ruben Lopez-Aladid, Roberto Cabrera, Albert Gabarrus, Andrea Palomeque, Patricia Oscanoa, Adrian Ceccato, Ana Motos, Rosanel Amaro, Thierry Bernardi, Christian Provot, Alba Soler-Comas, Laura Munoz, Jordi Vila, Antoni Torres
Summary: The aim of this study was to validate and identify additional applications of the BioFilm Ring Test (BRT) for Pseudomonas aeruginosa (PA) isolates from patients with bronchiectasis (BE), and evaluate its relationship with antimicrobial stewardship. The results showed that a BPI value below 14.75 at 5 hours predicted the mucoid PA phenotype. In conclusion, the BRT has the potential to reveal biofilm features with clinical implications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Hong Chen, Peng-Cheng Ji, Yue-Heng Qi, Shi-Jin Chen, Chang-Yao Wang, Yu-Jie Yang, Xin-Yu Zhao, Jin-Wei Zhou
Summary: This study evaluated the synergistic effect of thymoquinone and nisin in reducing biofilm formation of P. aeruginosa on lettuce, and investigated their anti-virulence and anti-biofilm mechanisms. Thymoquinone inhibited the production of autoinducers and virulence factors, and enhanced the susceptibility of P. aeruginosa biofilms to nisin. It disrupted the quorum sensing system, altered cell membrane component, and down-regulated the expressions of genes related to virulence, efflux pump, and antioxidation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biophysics
Wing-Hin Lee, Ramin Rohanizadeh, Ching-Yee Loo
Summary: This study developed a novel bioactive bone substitute with improved anti-biofilm activity by functionalizing with curcumin, which effectively inhibits biofilm formation and controls viable planktonic cells. Curcumin was successfully incorporated onto HA and sustainably released over 14 days, showing stronger anti-biofilm effect against Staphylococcus aureus. The curcumin-loaded HA composite could be an alternative antimicrobial agent to control the risk of infections in postsurgical implants.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Ecology
Jose F. Garcia-Mazcorro, Stephany A. Castillo-Carranza, Blake Guard, Jose P. Gomez-Vazquez, Scot E. Dowd, Donald J. Brigthsmith
Article
Microbiology
Richard W. McLaughlin, Patricia Lynn Shewmaker, Anne M. Whitney, Ben W. Humrighouse, Ana C. Lauer, Vladimir N. Loparev, Christopher A. Gulvik, P. A. Cochran, Scot E. Dowd
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2017)
Article
Parasitology
Saban Tekin, Scot E. Dowd, Marko Davinic, Ahmet Bursali, Adem Keskin
PARASITOLOGY RESEARCH
(2017)
Article
Infectious Diseases
Shahid Karim, Khemraj Budachetri, Nabanita Mukherjee, Jaclyn Williams, Asma Kausar, Muhammad Jawadul Hassan, Steven Adamson, Scot E. Dowd, Dmitry Apanskevich, Abdullah Arijo, Zia Uddin Sindhu, Muhammad Azam Kakar, Raja Muhammad Dilpazir Khan, Shafiq Ullah, Muhammad Sohail Sajid, Abid Ali, Zafar Iqbal
PLOS NEGLECTED TROPICAL DISEASES
(2017)
Article
Multidisciplinary Sciences
Amanda L. Fuchs, Alan J. Weaver, Brian P. Tripet, Mary Cloud B. Ammons, Martin Teintze, Valerie Copie
Article
Allergy
Jan Hubert, Marta Nesvorna, Pavel Klimov, Scot E. Dowd, Bruno Sopko, Tomas Erban
Article
Biochemistry & Molecular Biology
Amanda L. Fuchs, Sage M. Schiller, Wyatt J. Keegan, Mary Cloud B. Ammons, Brian Eilers, Brian Tripet, Valerie Copie
Article
Cell Biology
Amanda L. Fuchs, Isaac R. Miller, Sage M. Schiller, Mary Cloud B. Ammons, Brian Eilers, Brian Tripet, Valerie Copie
Article
Cell Biology
Catherine B. Anders, Tyler M. W. Lawton, Hannah L. Smith, Jamie Garret, Margaret M. Doucette, Mary Cloud B. Ammons
Summary: The study utilized an ex vivo model to generate 6 distinct functional phenotypes of macrophages, profiling them based on cell surface markers, secreted proteins, gene expression, and metabolites. The research identified unique metabolic profiles for each phenotype, grouping them into inflammatory and wound resolution categories, with key differences observed in metabolic pathways. Ultimately, the integration of metabolomics provided a comprehensive understanding of macrophage diversity and functional plasticity.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Microbiology
Jan Hubert, Marta Nesvorna, Pavel B. Klimov, Tomas Erban, Bruno Sopko, Scot E. Dowd, Erin D. Scully
Summary: Through correlations between host and symbiont gene expression profiles, important molecular pathways potentially regulating or facilitating their interactions were identified. Cardinium gene expression explained 95% of the variation in mite gene expression, while mite gene expression explained 76% of Cardinium gene expression variability. Cardinium affects mite metabolic pathways and is correlated with genes associated with terpenoid biosynthesis.
Review
Nutrition & Dietetics
Jeffrey L. Ward, Moises Torres-Gonzalez, Mary Cloud B. Ammons
Summary: The association of hyperinflammation and hyperferritinemia with adverse outcomes in SARS-CoV-2-infected patients suggests the importance of iron homeostasis in pathogenesis. Bovine lactoferrin, a multipurpose glycoprotein, has the potential to be a therapeutic adjuvant against SARS-CoV-2.
Article
Gastroenterology & Hepatology
Toufic A. Kabbani, Kumar Pallav, Scot E. Dowd, Javier Villafuerte-Galvez, Rohini R. Vanga, Natalia E. Castillo, Joshua Hansen, Melinda Dennis, Daniel A. Leffler, Ciaran P. Kelly
Article
Immunology
Tamar L. Gur, Lena Shay, Aditi Vadodkar Palkar, Sydney Fisher, Vanessa A. Varaljay, Scot Dowd, Michael T. Bailey
BRAIN BEHAVIOR AND IMMUNITY
(2017)
Article
Microbiology
Shukria Akbar, Scot E. Dowd, D. Cole Stevens
GENOME ANNOUNCEMENTS
(2017)
Article
Microbiology
Barbara I. Adaikpoh, Scot E. Dowd, D. Cole Stevens
GENOME ANNOUNCEMENTS
(2017)
Review
Infectious Diseases
Allison M. Hitchcock, Wesley D. Kufel, Keri A. Mastro Dwyer, Eric F. Sidman
Summary: Lenacapavir is a novel HIV-1 treatment option for patients with multidrug-resistant (MDR) HIV-1 infection. It has a favorable pharmacokinetic profile and has shown good tolerability and efficacy in clinical trials.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)
Article
Infectious Diseases
Roberta Gagliardini, Alessandro Tavelli, Stefano Rusconi, Sergio Lo Caputo, Vincenzo Spagnuolo, Maria Mercedes Santoro, Andrea Costantini, Alessandra Vergori, Franco Maggiolo, Andrea Giacomelli, Giulia Burastero, Giordano Madeddu, Eugenia Quiros Roldan, Antonella d'Arminio Monforte, Andrea Antinori, Alessandro Cozzi-Lepri
Summary: This study evaluated multiple treatment failures to modern antiretroviral therapy in HIV-infected individuals and found that approximately 4% of them were difficult to treat. The difficult to treat group, compared to the non-difficult to treat group, was characterized by older age, higher prevalence of AIDS, lower CD4+ cell count, and higher risk of treatment failure.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2024)