Article
Chemistry, Inorganic & Nuclear
Mostafa A. Sayed, M. A. Ahmed, M. F. El-Shahat, Islam M. El-Sewify
Summary: In this study, mesoporous polyaniline/SnO2 nanoparticles were prepared using a cost-effective sol-gel method with chitosan as a structure directing agent. The incorporation of polyaniline on the surface of SnO2 enhanced the photocatalytic response and increased the surface area and the number of active sites. The PANI/SnO2 nanocomposite showed higher photocatalytic reactivity in the degradation of RhB dye under the same reaction conditions. It also enhanced the photocatalytic response in the visible region and reduced the recombination of electron-hole pairs.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Ji Yoon Lim, Soo Suk Lee
Summary: In this study, we developed quartz crystal microbalance immunosensors for the sensitive detection of alpha-fetoprotein in human serum. By using gold and titanium dioxide nanoparticles, we employed three signal amplification strategies to enhance the detection capability of the QCM immunosensor. Gold staining of gold nanoparticles and photocatalytic silver staining of titanium dioxide nanoparticles were effective methods for size expansion and mass enhancement, respectively. The detection limits achieved using these signal amplification techniques were significantly improved compared to traditional assays.
Article
Chemistry, Medicinal
Sandesh Deshpande, Wenjuan Huo, Rinu Shrestha, Kevin Sparrow, James M. Wood, Gary B. Evans, Lawrence D. Harris, Richard L. Kingston, Esther M. M. Bulloch
Summary: Galidesivir, an adenosine nucleoside analog, has been shown to inhibit the replication of dengue and Zika viruses in cell-based assays and animal models. In this study, the researchers directly tested the effects of Galidesivir on the polymerase activity of dengue-2 and Zika viruses. The results suggest that Galidesivir's inhibition of viral replication may not be due to the early termination of RNA synthesis.
ACS INFECTIOUS DISEASES
(2023)
Article
Plant Sciences
Yu Nakagawa, Shintaro Kakihara, Kazue Tsuzuki, Makoto Ojika, Yasuhiro Igarashi, Yukishige Ito
Summary: PRM-EA, a derivative of PRM-A, shows little aggregation in neutral aqueous media and retains binding specificity for Man. It stains glycoproteins selectively with terminal Man residues, demonstrating potential as a unique staining agent for glycoproteins detection.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Yurie Tehee Kim, Hyesoo Oh, Myung Jun Seo, Dong Hyeun Lee, Jieun Shin, Serang Bong, Sujeong Heo, Natalia Diyah Hapsari, Kyubong Jo
Summary: This study constructed 21 FP-DBPs to stain and visualize DNA molecules, with the choice of FP and DBP affecting the brightness and stretching of DNA staining, providing guidance for the development of novel DNA-binding fluorescent proteins.
Article
Multidisciplinary Sciences
Elena Y. Trizna, Aleksandr M. Sinitca, Asya I. Lyanova, Diana R. Baidamshina, Pavel V. Zelenikhin, Dmitrii I. Kaplun, Airat R. Kayumov, Mikhail I. Bogachev
Summary: Differential fluorescent staining is a useful tool in standard microscopic imaging protocols for visualizing, segmenting and quantifying cells and cellular structures. However, the incompatibility of staining agents with viable cells limits its applicability in live experiments, requiring sample extraction and increasing laboratory costs. Therefore, the development of computerized image analysis methods for segmenting and quantifying cells from plain monochromatic images is of great interest. The enclosed dataset provides a test bed for validating image analysis methods using human colon adenocarcinoma Caco-2 cells under different imaging conditions.
Article
Chemistry, Analytical
Mao-Hua Wang, Wei-Long Cui, Yun-Hao Yang, Jian-Yong Wang
Summary: In this study, a new fluorescent chemo-sensor Couoxo-LD was developed for effective labeling of intracellular lipid droplets. The probe showed positive sensitivity to polarity and viscosity, and demonstrated excellent selectivity, pH stability, and low biotoxicity for cellular imaging and in vivo imaging.
Article
Multidisciplinary Sciences
Hiroyuki Sasaki, Hisako Arai, Emi Kikuchi, Hideki Saito, Keiko Seki, Takeshi Matsui
Summary: The use of Mayer's hematoxylin followed by Reynold's lead citrate solution is suggested as a reliable and promising alternative to Uranyl acetate (UA) staining for ultrathin sections in biological electron microscopy.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
David Donnermeyer, Sina Schmidt, Arno Rohrbach, Johannes Berlandi, Sebastian Buerklein, Edgar Schaefer
Summary: The study tested the suitability of rhodamine B staining of root canal sealers but found it to overestimate the penetration of sealers into dentinal tubules.
Article
Environmental Sciences
Huiyan Tong, Qianyi Jiang, Xiaocong Zhong, Xingshuai Hu
Summary: This study introduces an effective Rhodamine B dye staining method for microplastics, enabling the visualization of different types of microplastic polymers tested in laboratory experiments, particularly transparent, white, and small-sized microplastics.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biophysics
Guangze Yang, Yun Liu, Chun-Xia Zhao
Summary: Labeling nanoparticles with fluorescent dyes is a common method to study their cell uptake and biodistribution for drug delivery applications. However, the assumption that fluorescence intensity correlates with the amount of nanoparticles taken up by cells may not always hold true due to factors like dye dispersion, quenching, and material shading. This study shows that both hydrophobic and hydrophilic dye-labeled nanoparticles can suffer from dye quenching, making quantitative comparison of cell uptake challenging. The proposed solution aims to enable direct comparative studies of dye-labeled nanoparticles for better design and evaluation in drug delivery.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Polymer Science
Krishnaroop Chaudhuri, Riddhiman Medhi, Zhenglin Zhang, Zhuoyun Cai, Christopher K. K. Ober, Jonathan T. T. Pham
Summary: In this study, the diffusion process of rhodamine B fluorescent dye in polystyrene-polydimethylsiloxane block copolymer coatings was visualized using confocal microscopy. The intensity of dye inside the coatings showed non-monotonic changes, attributed to concurrent dye molecule transport in different directions. An empirical fitting equation was used to compare contamination rates between copolymers, indicating that dye penetration is influenced by the chemical makeup and configuration of the BCPs. The research highlights the utility of confocal microscopy in visualizing the transport of fluorophores within coatings.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Review
Chemistry, Inorganic & Nuclear
Liu Yang, Qingxin Chen, Zhanchen Wang, Huatang Zhang, Hongyan Sun
Summary: This article summarizes the representative small molecular fluorescent probes reported in the last decade and categorizes them into four groups based on their functional membrane-anchoring moieties. The mechanisms to achieve membrane-specific fluorescence staining, including membrane targeting group approach, aggregation induced emission, disassembly light up fluorescence strategy, and restricted intramolecular rotation, are extensively discussed. Furthermore, membrane-targeting probes for super-resolution imaging of membrane dynamics are presented as an emerging technology.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
H. Samet Varol, Stefan Seeger
Summary: This study presents a new method to collect 3D confocal images of irregular polysiloxane micro- and nanorods for discussing their wetting response and quantifying their dimensions. Through different staining scenarios, the researchers were able to resolve the micro- and nanorods and validate the results against electron microscopy images. The findings from the 3D confocal images shed light on the long-time existence of multiple wetting states and the shifting and pinning of three-phase contact lines under the same droplet on surfaces.
Article
Computer Science, Artificial Intelligence
Debamita Kumar, Pradipta Maji
Summary: This study introduces a novel approach for automatic recognition of staining patterns in HEp-2 cells, utilizing a Rough-Bayesian model to evaluate descriptor relevance and a support vector machine to predict staining patterns. The proposed method outperforms state-of-the-art methods and significantly improves the accuracy of classifying HEp-2 cell images.
PATTERN RECOGNITION
(2021)
Article
Biochemistry & Molecular Biology
Yeon Soo Kim, Piyushi Gupta Vallur, Victoria M. Jones, Beth L. Worley, Sara Shimko, Dong-Hui Shin, LaTaijah C. Crawford, Chi-Wei Chen, Katherine M. Aird, Thomas Abraham, Trevor G. Shepherd, Joshua Warrick, Nam Y. Lee, Rebecca Phaeton, Karthikeyan Mythreye, Nadine Hempel
Article
Critical Care Medicine
David A. Jolliffe, Christos Stefanidis, Zhican Wang, Nazanin Z. Kermani, Vassil Dimitrov, John H. White, John E. McDonough, Wim Janssens, Paul Pfeffer, Christopher J. Griffiths, Andrew Bush, Yike Guo, Stephanie Christenson, Ian M. Adcock, Kian Fan Chung, Kenneth E. Thummel, Adrian R. Martineau
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Patricia Silveyra, Nathalie Fuentes, Deborah Montes, Amy Spinelli, Thomas Abraham, Indira Purushothaman, Zissis Chroneos
Article
Endocrinology & Metabolism
Deepak Kumar Khajuria, Marwa Soliman, John C. Elfar, Gregory S. Lewis, Thomas Abraham, Fadia Kamal, Reyad A. Elbarbary
Article
Nanoscience & Nanotechnology
Thomas Abraham, Christopher O. McGovern, Samuel S. Linton, Zachary Wilczynski, James H. Adair, Gail L. Matters
Summary: Aptamer-targeted nanoparticles loaded with imaging agents showed enhanced detection of pancreatic and prostate tumors in vivo and ex vivo. The specific interaction with CCK-B receptors allows for well-distributed distribution of the tumor-targeted nanoparticles in the tumor matrix, improving early cancer detection.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Brett A. Eyford, Chaahat S. B. Singh, Thomas Abraham, Lonna Munro, Kyung Bok Choi, Tracy Hill, Rhonda Hildebrandt, Ian Welch, Timothy Z. Vitalis, Reinhard Gabathuler, Jacob A. Gordon, Hans Adomat, Emma S. T. Guns, Chieh-Ju Lu, Cheryl G. Pfeifer, Mei Mei Tian, Wilfred A. Jefferies
Summary: The study shows that the MTfp-NOX4 POC can cross the BBB, downregulate NOX4 expression, and have therapeutic effects on ischemic stroke, providing new potential treatment options for challenging neuropathologies.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Correction
Biochemistry & Molecular Biology
Brett A. Eyford, Chaahat S. B. Singh, Thomas Abraham, Lonna Munro, Kyung Bok Choi, Tracy Hill, Rhonda Hildebrandt, Ian Welch, Timothy Z. Vitalis, Reinhard Gabathuler, Jacob A. Gordon, Hans Adomat, Emma S. T. Guns, Chieh-Ju Lu, Cheryl G. Pfeifer, Mei Mei Tian, Wilfred A. Jefferies
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Vivien Beziat, Franck Rapaport, Jiafen Hu, Matthias Titeux, Mathilde Bonnet des Claustres, Mathieu Bourgey, Heather Griffin, Elise Bandet, Cindy S. Ma, Roya Sherkat, Hassan Rokni-Zadeh, David M. Louis, Majid Changi-Ashtiani, Ottavia M. Delmonte, Toshiaki Fukushima, Tanwir Habib, Andrea Guennoun, Taushif Khan, Noemi Bender, Mahbuba Rahman, Fredegonde About, Rui Yang, Geetha Rao, Claire Rouzaud, Jingwei Li, Debra Shearer, Karla Balogh, Fatima Al Ali, Manar Ata, Soroosh Dabiri, Mana Momenilandi, Justine Nammour, Marie-Alexandra Alyanakian, Marianne Leruez-Ville, David Guenat, Marie Materna, Lea Marcot, Natasha Vladikine, Christine Soret, Hassan Vahidnezhad, Leila Youssefian, Amir Hossein Saeidian, Jouni Uitto, Emilie Catherinot, Shadi Sadat Navabi, Mohammed Zarhrate, David T. Woodley, Mohamed Jeljeli, Thomas Abraham, Serkan Belkaya, Lazaro Lorenzo, Jeremie Rosain, Mousa Bayat, Fanny Lanternier, Olivier Lortholary, Faramarz Zakavi, Philippe Gros, Gerard Orth, Laurent Abel, Jean-Luc Pretet, Sylvie Fraitag, Emmanuelle Jouanguy, Mark M. Davis, Stuart G. Tangye, Luigi D. Notarangelo, Nico Marr, Tim Waterboer, David Langlais, John Doorbar, Alain Hovnanian, Neil Christensen, Xavier Bossuyt, Mohammad Shahrooei, Jean-Laurent Casanova
Summary: The study investigated a patient with HPV-2-driven "tree-man" phenotype and two relatives with severe HPV4-driven warts, revealing the relationship between a CD28 variant and T cell responses. Findings suggest that T cell responses are important in controlling HPV infections.
Article
Neurosciences
Chaahat S. B. Singh, Brett A. Eyford, Thomas Abraham, Lonna Munro, Kyung Bok Choi, Mark Okon, Timothy Z. Vitalis, Reinhard Gabathuler, Chieh-Ju Lu, Cheryl G. Pfeifer, Mei Mei Tian, Wilfred A. Jefferies
Summary: The study identified a 12-amino acid peptide, MTfp, capable of crossing the BBB and entering brain cells, opening up new potential avenues for treating neuropathologies.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Critical Care Medicine
Laurens J. De Sadeleer, John E. McDonough, Jonas C. Schupp, Xiting Yan, Arno Vanstapel, Anke Van Herck, Stephanie Everaerts, Vincent Geudens, Annelore Sacreas, Tinne Goos, Celine Aelbrecht, Tim S. Nawrot, Dries S. Martens, Dominique Schols, Sandra Claes, Johny A. Verschakelen, Eric K. Verbeken, Maximilian Ackermann, Anabelle Decottignies, Manon Mahieu, Tillie-Louise Hackett, James C. Hogg, Bart M. Vanaudenaerde, Stijn E. Verleden, Naftali Kaminski, Wim A. Wuyts
Summary: This study identified molecular traits that characterize the morphological progression of fibrotic hypersensitivity pneumonitis (fHP) and their associations with clinical behavior. Comparing with idiopathic pulmonary fibrosis (IPF), the transcriptome landscape is determined by local disease extent rather than diagnosis alone.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Michael Chute, Preetinder K. Aujla, Yingxi Li, Sayantan Jana, Pavel Zhabyeyev, Jaslyn Rasmuson, Caroline A. Owen, Thomas Abraham, Gavin Y. Oudit, Zamaneh Kassiri
Summary: ADAM15 plays an important role in heart disease, and its deficiency can lead to impaired scar formation after myocardial infarction, potentially related to collagen cross-linking and kinase expression.
Article
Multidisciplinary Sciences
Emmanuel K. Teye, Shasha Lu, Fangyuan Chen, Wenrui Yang, Thomas Abraham, Douglas B. Stairs, Gregory S. Yochum, Robert A. Brodsky, Jefrey J. Pu
Summary: PIGN, as a regulator of the spindle assembly checkpoint, affects the activation of mitotic checkpoint in leukemic cells, which in turn influences chromosome segregation, suggesting a crucial role in suppressing chromosomal instability associated with leukemia.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Natalie K. Yoshioka, Gregory M. Young, Deepak Kumar Khajuria, Vengadeshprabhu Karuppagounder, William J. Pinamont, Julie C. Fanburg-Smith, Thomas Abraham, Reyad A. Elbarbary, Fadia Kamal
Summary: This study investigated the changes in collagen fibers in osteoarthritis using a mouse model. The researchers found that the thickness and formation of collagen fibers were altered in the articular cartilage, subchondral bone, and menisci of mice with osteoarthritis. They also observed changes in the expression of collagen I and II in these tissues. These findings provide a comprehensive understanding of the microarchitectural changes in the extracellular matrix during osteoarthritis and can guide future studies on therapies targeting collagen changes to halt the progression of the disease.
SCIENTIFIC REPORTS
(2022)
Article
Ophthalmology
Kuniyoshi Kanai, Meredith Whiteside, Michael Wong, Tammy La, Maryam Nassiri, Sam Lee, Sze Kei Yeung, Adrienne Coulter, Mareena Roufail, Kevin Ruder, Cindi Chen, David Liu, Thomas Abraham, Armin M. Hinterwirth, Thomas Lietman, Thuy D. Doan, Gerami Seitzman
Summary: This study uses unbiased deep sequencing to identify causative pathogens of acute infectious conjunctivitis, potentially allowing for improved approaches to diagnosis and management.
OPTOMETRY AND VISION SCIENCE
(2023)
Proceedings Paper
Biochemical Research Methods
Thomas Abraham, K. Rebecca Koob, Nurgul Carkaci-Salli, Umur Aydogan
IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES XVIII
(2020)