Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Steven Vermeulen, Bart Van Puyvelde, Laura Bengtsson del Barrio, Ruben Almey, Bernard K. K. van der Veer, Dieter Deforce, Maarten Dhaenens, Jan de Boer
Summary: This study investigates how surface topography influences nuclear morphology, histone modifications, and gene expression. The authors find that micro-topographies result in a loss of histone acetylation and nucleoli abundance, as well as a reduction in gene expression associated with chromosome organization. They also show that micro-topographies can influence the proliferation and phenotype of mesenchymal stem cells, and this effect is reversible.
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Review
Cell & Tissue Engineering
Kevin Hayes, Yun-Kyo Kim, Martin F. Pera
Summary: Nodal is a crucial factor in mammalian embryonic development and the maintenance of pluripotent stem cells. Its signaling pathway may play an important role in regulating self-renewal in hPSC.
Review
Biochemistry & Molecular Biology
Ines Milagre, Carolina Pereira, Raquel A. Oliveira
Summary: Human pluripotent stem cells (PSCs), including embryonic and induced pluripotent stem cells, are widely used in biomedical research for understanding development, disease progression, and drug discovery. However, concerns have been raised regarding their safety in regenerative medicine, particularly due to the occurrence of aneuploid cells resulting from errors in chromosome segregation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Erin M. MacFarlane, Jennifer E. Bruin
Summary: The prevalence of diabetes is increasing globally, with a reported association between diabetes and environmental pollutant exposure. Identifying chemicals that disrupt metabolism, particularly those affecting pancreatic endocrine cells, is a high priority. Human pluripotent stem cells show promise as a tool for in vitro toxicity testing and screening for chemicals influencing endocrine cell formation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Sajedeh Nasr Esfahani, Agnes M. Resto Irizarry, Xufeng Xue, Samuel Byung-Deuk Lee, Yue Shao, Jianping Fu
Summary: Research on controlling the fate of human pluripotent stem cells is challenging, but micro/nanoengineering technologies bring new hope. Biochemical and biomechanical cues play crucial roles in determining the behavior of human pluripotent stem cells.
Review
Cell & Tissue Engineering
Azam Shamsian, Roxana Sahebnasagh, Amir Norouzy, Safin Hassan Hussein, Mohammad Hossein Ghahremani, Zahra Azizi
Summary: Induced pluripotent stem cells (iPSCs) have wide-ranging potential applications in medical research, and cancer cells may serve as a new source for generating iPSCs. Although cancer cells share similarities with iPSCs, they carry genetic abnormalities and pose a risk of tumor formation. This review discusses the feasibility of using cancer cells as a preferred source for iPSC generation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Medicine, Research & Experimental
Edit Gara, Eleonora Zucchelli, Annamaria Nemes, Zoltan Jakus, Kitti Ajtay, Eva Kemecsei, Gabor Kiszler, Nikolett Hegedus, Krisztian Szigeti, Ivan Foldes, Kristof Arvai, Janos Kosa, Kraszimir Kolev, Erzsebet Komorowicz, Parasuraman Padmanabhan, Pal Maurovich-Horvat, Edit Dosa, Gyorgy Varady, Miklos Polos, Istvan Hartyanszky, Sian E. Harding, Bela Merkely, Domokos Mathe, Gabor Szabo, Tamas Radovits, Gabor Foldes
Summary: This study demonstrates that the combination of human induced pluripotent stem cell-derived endothelial cells (hPSC-EC) and biomatrices has the potential to repair ischemic tissues by reconstructing vascular structures and functions. In vitro and in vivo experiments showed that these cells can develop beneficial properties such as antifibrotic, vasoactive and anti-inflammatory effects during recellularization.
Article
Biochemistry & Molecular Biology
Eirini Filidou, Leonidas Kandilogiannakis, Gesthimani Tarapatzi, Michail Spathakis, Colin Su, Alin Rai, David W. Greening, Konstantinos Arvanitidis, Vasilis Paspaliaris, George Kolios
Summary: Pluripotent stem cells play a vital role in regenerative medicine. However, the limited availability and ethical concerns associated with embryonic pluripotent stem cells have led to the discovery of very small embryonic-like (VSEL) stem cells. In this study, a simplified and effective method for isolating small pluripotent stem cells from human peripheral blood is presented. The isolated small blood stem cells (SBSC) population expresses pluripotency markers and also exhibits characteristics of hematopoietic and mesenchymal stem cells. Proteomic profiling reveals the presence of various stem cell markers and transcription regulatory complex factors in SBSCs. This novel isolation process yields a abundant population of small-sized cells with pluripotent characteristics from human peripheral blood.
Review
Cell & Tissue Engineering
Samaneh Dehghan, Reza Mirshahi, Alireza Shoae-Hassani, Masood Naseripour
Summary: This study discusses age-related macular degeneration (AMD) and the current treatment options for wet and dry AMD. Stem cell therapy has shown great potential in the treatment of AMD. Human pluripotent stem cells, especially induced pluripotent stem cells (iPSCs), can be used to generate retinal pigment epithelium (RPE) cells, restoring the structure and function of the retina.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Peggie Chien, Haibin Xi, April D. Pyle
Summary: This review describes the current state of human pluripotent stem cells (hPSCs) in recapitulating human myogenesis ex vivo, and discusses the potential of differentiating hPSCs into muscle stem cells for regenerative medicine, as well as considerations of stem cell and progenitor cell state and function.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Materials Science, Biomaterials
Peiran Zhu, Jamar Hawkins, Will Hamilton Linthicum, Menglin Wang, Ningwei Li, Nanjia Zhou, Qi Wen, Alicia Timme-Laragy, Xiaofei Song, Yubing Sun
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Review
Developmental Biology
Jamar Hawkins, Xiaosu Miao, Wei Cui, Yubing Sun
Summary: Mechanical biomarkers are gaining attention in assessing embryo morphology and promoting healthy preimplantation embryo development, with evidence suggesting their effectiveness as early indicators of developmental potential comparable to embryo morphology. Future research can explore the use of biomimetic mechanical cues to improve embryo culture systems.
MOLECULAR HUMAN REPRODUCTION
(2021)
Review
Chemistry, Physical
Jianping Fu, Aryeh Warmflash, Matthias P. Lutolf
Summary: This article highlights the importance of stem-cell-derived embryo models in advancing our understanding of mammalian embryology, emphasizing the significance of simulating mouse and human development in current models and utilizing bioengineering tools.
Article
Biochemical Research Methods
Yi Zheng, Yue Shao, Jianping Fu
Summary: This study presents a microfluidic device for modeling human embryo development, derived from human pluripotent stem cells. The resulting structures exhibit molecular and cellular features resembling early post-implantation human embryo development. The microfluidic model is compatible with high-throughput studies and various analytical techniques.
Article
Multidisciplinary Sciences
Dennis W. Zhou, Marc A. Fernandez-Yaguee, Elijah N. Holland, Andres F. Garcia, Nicolas S. Castro, Eric B. O'Neill, Jeroen Eyckmans, Christopher S. Chen, Jianping Fu, David D. Schlaepfer, Andres J. Garcia
Summary: The coupling of traction force and FAK signaling coordinates cell migration and tissue-scale forces to promote microtissue repair. This process relies on actomyosin contractility, talin-FAK binding, and vinculin to convert forces into FAK signaling events regulating cell migration and tissue repair.
NATURE COMMUNICATIONS
(2021)
Article
Materials Science, Biomaterials
Ningwei Li, Feiyu Yang, Subiksha Parthasarathy, Sarah St Pierre, Kelly Hong, Narciso Pavon, ChangHui Pak, Yubing Sun
Summary: Recent advancements in utilizing human pluripotent stem cell-derived in vitro models for studying early human development have raised questions about the ability of extrinsic morphogen gradients to pattern neuroectoderm tissues. A new device design, known as localized passive diffusion (LPaD), has been developed to generate stable chemical gradients without the need for continuous pumping or gel encapsulation. This device has been successfully used to induce cellular responses to small-molecule drug gradients and pattern hPSC-derived forebrain neuroepithelial cells with sonic hedgehog (Shh) signal gradients.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Biology
Tianfa Xie, Sarah R. St Pierre, Nonthakorn Olaranont, Lauren E. Brown, Min Wu, Yubing Sun
Summary: In this study, we observed that rat embryonic fibroblasts (REF) confined in circular mesoscale patterns can transition from spindle shapes to more compact morphologies when in mechanical equilibrium. Radial cell alignment only occurs at the pattern boundary in this state, and is influenced by factors such as cell contractility and cell-cell adhesion. The presence of an actin gradient with isotropic meshwork suggests the existence of a stiffness gradient in the confined REF monolayers.
Article
Materials Science, Multidisciplinary
Tiankai Zhao, Yubing Sun, Qionghua Zhu, Xin Li, Mehdi Baghaee, Yuenan Wang, Hongyan Yuan
Summary: Reaction-diffusion models are widely used to study pattern formation in developmental biology, but traditional equations cannot explain concentric ring distributions of cell types; it is suggested that circular patterns are the result of coupling mechanobiological factors with reaction-diffusion models.
JOURNAL OF THE MECHANICS AND PHYSICS OF SOLIDS
(2021)
Review
Developmental Biology
Aidan H. Terhune, Jeyoon Bok, Shiyu Sun, Jianping Fu
Summary: The development of a zygote into an embryo is a miraculous process in nature, but scientists have struggled to understand the mechanisms in human embryos due to ethical and practical limitations. However, pluripotent stem cells have the ability to mimic specific embryonic events, allowing the creation of in vitro models that recapitulate different stages of early embryo development.
Article
Multidisciplinary Sciences
Hongyan Gao, Feiyu Yang, Kianoosh Sattari, Xian Du, Tianda Fu, Shuai Fu, Xiaomeng Liu, Jian Lin, Yubing Sun, Jun Yao
Summary: This study demonstrates integrated nanoelectronic sensors capable of simultaneously probing electrical and mechanical cellular responses. The sensors offer advantages in tracking and differentiating cell states, as well as decoding vector information in cellular motion. Through two-in-one converging integration, the sensors enable improved bioelectronic detections and reduced invasiveness.
Article
Chemistry, Multidisciplinary
Yen-Yu Hsu, Agnes M. Resto Irizarry, Jianping Fu, Allen P. Liu
Summary: Plasma membrane tension acts as a regulator of cellular activities, but current measurement techniques are limited in their ability to investigate membrane biophysics and the role of tension. In this study, a new optical reporter for membrane tension is developed using a modified E. coli mechanosensitive channel and circularly permuted GFP, which allows measurement of membrane tension based on fluorescence intensity changes.
Article
Multidisciplinary Sciences
Rebecca Sebastian, Kang Jin, Narciso Pavon, Ruby Bansal, Andrew Potter, Yoonjae Song, Juliana Babu, Rafael Gabriel, Yubing Sun, Bruce Aronow, ChangHui Pak
Summary: This study investigates the effects of NRXN1 gene deletions on brain development using human pluripotent stem cell-derived forebrain organoid models. The results show that NRXN1 deletions specifically affect the developmental trajectories of early neural progenitors and result in disease-specific transcriptomic signatures. Additionally, the deletions lead to synaptic dysfunction in the neuronal networks.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Feiyu Yang, Pengcheng Chen, Han Jiang, Tianfa Xie, Yue Shao, Deok-Ho Kim, Bo Li, Yubing Sun
Summary: Strain gradients play a crucial role in directional cell migration. This study develops a programmable uniaxial cell stretch device to create controllable strain gradients and demonstrates that these gradients are sufficient to drive over 60% of rat embryonic fibroblasts towards the lower strain side. Focal adhesion analysis and a motor-clutch model suggest that strain-introduced traction force and integrin fibronectin pairs' catch-release dynamics are responsible for such directional migration.
Article
Multidisciplinary Sciences
Feng Lin, Xia Li, Shiyu Sun, Zhongyi Li, Chenglin Lv, Jianbo Bai, Lin Song, Yizhao Han, Bo Li, Jianping Fu, Yue Shao
Summary: Researchers have developed an efficient biomaterial system that can generate gut spheroids from human pluripotent stem cells through mechanically enhanced tissue morphogenesis. The study also reveals the mechanisms behind gut spheroid formation, including the effects of cell multilayering and crowding, and proposes a mechanism for morphogenetic transitions.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Bin Zhao, Ningwei Li, Tianfa Xie, Yousef Bagheri, Chungwen Liang, Puspam Keshri, Yubing Sun, Mingxu You
