Article
Gastroenterology & Hepatology
Ziyu Yang, Juanxiu Qin, Lina Zhao, Tianchi Chen, Qian Huang, Ying Jian, Qi Zhao, Sheng Yang, Qi Li, Qian Liu, Michael Otto, Min Li
Summary: Inflammatory bowel disease (IBD) is a globally increasing gastrointestinal inflammatory disorder. Patients with IBD have an increased risk of Clostridioides difficile infection (CDI), which worsens the clinical outcome of IBD. The underlying reasons for this are still poorly understood.
Review
Gastroenterology & Hepatology
Mei Bai, Hong Guo, Xiao-Yao Zheng
Summary: This paper summarizes and presents the epidemiology, pathophysiology, clinical manifestation, prevention, and therapy of Clostridium difficile infection (CDI) in inflammatory bowel disease (IBD).
THERAPEUTIC ADVANCES IN GASTROENTEROLOGY
(2023)
Review
Gastroenterology & Hepatology
Tamara Alhobayb, Matthew A. Ciorba
Summary: This review provides a comprehensive overview of the epidemiology, diagnosis, and treatment of symptomatic Clostridium difficile infection (CDI) in inflammatory bowel disease (IBD) patients. CDI can complicate IBD management and lead to disease flares, recurrent episodes, and prolonged hospital stays. Prompt diagnosis and treatment, as well as prevention strategies, are crucial in managing CDI in IBD patients.
CURRENT OPINION IN GASTROENTEROLOGY
(2023)
Article
Infectious Diseases
S. Scott Sutton, Joseph Magagnoli, Tammy H. Cummings, James W. Hardin
Summary: The results of this study demonstrate a decreased risk of recurrent CDI in patients exposed to melatonin. This suggests that melatonin may have potential benefits as an antimicrobial adjuvant and anti-inflammatory agent in the management of recurrent CDI.
Review
Health Care Sciences & Services
Elena Iana, Catalin Boboc, Andreea Gabriela Vlad, Maria Teodora Cosoreanu, Malina Anghel, Anca Andreea Boboc, Andreea Ioan, Mara Ioana Ionescu, Liana Gavriliu, Felicia Galos
Summary: Children with inflammatory bowel disease are more susceptible to Clostridium difficile infection, with ulcerative colitis patients being more affected than Crohn's disease patients. Managing pediatric patients with both IBD and CDI is challenging and requires a multidisciplinary approach. Treatment for underlying IBD may need to be escalated after CDI treatment.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Review
Medicine, Research & Experimental
Qichen Shen, Bingbai Xu, Caihong Wang, Yingping Xiao, Yuanxiang Jin
Summary: Inflammatory bowel disease (IBD) is a chronic intestinal inflammation with no cure. Disruption of the microbial ecosystem in the intestine is considered a key factor in IBD. Bacterial membrane vesicles (MVs) play important roles in regulating intestinal microecology and have potential therapeutic applications in IBD patients.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Infectious Diseases
Puo-Hsien Le, Cheng-Tang Chiu, Pai-Jui Yeh, Yu-Bin Pan, Cheng-Hsun Chiu
Summary: This study retrospectively evaluated the role of Clostridium innocuum (CI) infection in patients with inflammatory bowel disease (IBD). The results showed that CI infection may lead to poorer clinical remission in ulcerative colitis, which should be considered in the treatment approach.
JOURNAL OF INFECTION
(2022)
Article
Multidisciplinary Sciences
Xin Li, Irina Leonardi, Gregory G. Putzel, Alexa Semon, William D. Fiers, Takato Kusakabe, Woan-Yu Lin, Iris H. Gao, Itai Doron, Alejandra Gutierrez-Guerrero, Meghan B. DeCelie, Guilhermina M. Carriche, Marissa Mesko, Chen Yang, Julian R. Naglik, Bernhard Hube, Ellen J. Scherl, Iliyan D. Iliev
Summary: The study reveals the rich genetic diversity of pathogenic Candida albicans strains in the colonic mucosa of patients with inflammatory bowel disease, with some strains having high immune-cell-damaging capacity (HD strains). These strains exacerbate intestinal inflammation in vivo through specific mechanisms, highlighting new insights into host-fungal interactions and potential diagnostic and therapeutic targets for inflammatory diseases.
Article
Gastroenterology & Hepatology
Pedro Palacios Argueta, Miguel Salazar, Bashar Attar, Roberto Simons-Linares, Bo Shen
Summary: Inflammatory bowel disease (IBD) patients have a higher risk of Clostridium difficile infection (CDI) after hospitalization for IBD flare. The 90-day CDI-specific readmission rate is 0.1%, with risk factors such as history of Parkinson's disease, prior CDI, HIV-positive status, and alcohol abuse disorder identified. Health care costs, charges, and burden for CDI-associated readmissions are high.
INFLAMMATORY BOWEL DISEASES
(2021)
Article
Pediatrics
Hyun-Woo Lee, Seung-Beom Han, Jung-Woo Rhim
Summary: This study aimed to determine the subjects for bacterial multiplex polymerase chain reaction (mPCR) testing and to interpret the mPCR test results based on patients' clinical symptoms and diagnoses. The study found that positive mPCR test results were significantly associated with older age, diagnosis of acute gastroenteritis, presence of hematochezia, and absence of cough. Additionally, the identification of invasive pathogens in patients with positive mPCR test results was significantly associated with the diagnosis of acute gastroenteritis, presence of fever and diarrhea, and higher C-reactive protein levels. Therefore, selective bacterial mPCR testing should be performed based on the patients' clinical symptoms and diagnoses.
Review
Biochemistry & Molecular Biology
Sandile Phinda Songca
Summary: Nanozymes are synthetic nanoparticulate materials that mimic biological activities of enzymes, used for detection and identification of pathogens efficiently, offering cost-effectiveness and stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Gastroenterology & Hepatology
Yanghua Liu, Kal Alnababtah, Simon Cook, Ying Yu
Summary: The study found that healthcare providers have relatively low knowledge of FMT, with main concerns being lack of clinical evidence and infection transmission. However, as knowledge increases, willingness to recommend FMT also increases. Strengthening FMT clinical efficacy and reducing infection can enhance its public acceptance, application, and popularity. Further research is required to explore the donor screening procedure.
THERAPEUTIC ADVANCES IN GASTROENTEROLOGY
(2021)
Article
Immunology
Si Yu, Xiaomeng Ge, Hui Xu, Bei Tan, Bowen Tian, Yujie Shi, Yimin Dai, Yue Li, Songnian Hu, Jiaming Qian
Summary: This study aimed to assess the gut microbiome and mycobiome in IBD patients with CDI. The results showed that IBD patients with CDI had decreased bacterial alpha diversity and significant differences in bacterial and fungal beta diversity compared to healthy individuals and IBD patients without CDI. Specific bacterial and fungal taxa were overrepresented in IBD patients with CDI. These findings provide insights into the pathological process and potential strategies for diagnosis and treatment in this subset of patients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Editorial Material
Gastroenterology & Hepatology
Heike E. F. Becker, John Penders, Daisy M. A. E. Jonkers
Article
Genetics & Heredity
Alaric W. D'Souza, Manish Boolchandani, Sanket Patel, Gianluca Galazzo, Jarne M. van Hattem, Maris S. Arcilla, Damian C. Melles, Menno D. de Jong, Constance Schultsz, Gautam Dantas, John Penders
Summary: The study found that international travel significantly increased the abundance and diversity of antimicrobial resistance (AMR) genes in the travelers' gut resistome, leading to the acquisition of various resistant genes. The shaping of the gut resistome by travel was closely associated with geographical destinations, with individuals returning from the same destination country more likely to have similar resistome features.
Article
Biochemistry & Molecular Biology
Armand M. A. Linkens, Niels van Best, Petra M. Niessen, Nicole E. G. Wijckmans, Erica E. C. de Goei, Jean L. J. M. Scheijen, Martien C. J. M. van Dongen, Christel C. J. A. W. van Gool, Willem M. de Vos, Alfons J. H. M. Houben, Coen D. A. Stehouwer, Simone J. M. P. Eussen, John Penders, Casper G. Schalkwijk
Summary: The study found that a 4-week diet low or high in AGEs has limited impact on the gut microbial composition of abdominally obese humans, with only Anaerostipes spp. showing a relative abundance difference. The habitual intake of dicarbonyls was not associated with microbial richness or general community structure, but the intake of 3-deoxyglucosone was linked to several genera abundances. Further investigation into the effects of dietary dicarbonyls on gut microbiota composition is warranted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Matthew Davies, Gianluca Galazzo, Jarne M. van Hattem, Maris S. Arcilla, Damian C. Melles, Menno D. de Jong, Constance Schultsz, Petra Wolffs, Alan McNally, Willem van Schaik, John Penders
Summary: Previous studies have shown a high risk of ESBL-producing Enterobacteriaceae acquisition among international travelers visiting AMR hotspots. This study investigated whether successful colonization of ESBL-E during travel is associated with the composition, functional capacity, and resilience of the traveler's microbiome. The results suggest that changes in the microbial composition and abundance of certain bacteria may play a role in preventing ESBL-E acquisition.
Article
Gastroenterology & Hepatology
Lars M. M. Vliex, Giang N. Le, Marina Fassarella, Dorien Reijnders, Gijs H. Goossens, Erwin G. Zoetendal, John Penders, Ellen E. Blaak
Summary: This study found that the effect of vancomycin treatment on tissue-specific insulin sensitivity and gut microbiota composition varied among individuals, depending on the presence of the vancomycin resistance gene vanB in their baseline gut microbiota.
Article
Gastroenterology & Hepatology
D. J. M. Barnett, M. F. Endika, C. E. Klostermann, F. Gu, C. Thijs, A. Nauta, H. A. Schols, H. Smidt, I. C. W. Arts, J. Penders
Summary: The infant gut microbiota is influenced by antibiotic exposures and prebiotic oligosaccharides found in breast milk. This study analyzed the stool microbiota of 1023 one-month-old infants and found that breastfeeding and antibiotic exposure both decrease gut microbial diversity, but have different effects on microbiota composition. Other factors such as C-section, homebirth, siblings, and exposure to animals also impact microbiota composition. Understanding these factors can help protect and promote a healthy infant gut microbiota.
Review
Biochemistry & Molecular Biology
Robert van Vorstenbosch, Hao Ran Cheng, Daisy Jonkers, John Penders, Erik Schoon, Ad Masclee, Frederik-Jan van Schooten, Agnieszka Smolinska, Zlatan Mujagic
Summary: Colorectal cancer (CRC) is associated with changes in volatile metabolic profiles, making it possible for non-invasive detection. However, the origin of these volatile organic compounds (VOCs) and their relation to gut microbiome are still not fully understood. This systematic review provides a comprehensive overview of the current understanding on this topic. The analysis of seventy-one included studies revealed dysregulations in various matrices, such as feces, urine, breath, blood, tissue, and saliva, with short-chain fatty acids, amino acids, proteolytic fermentation products, and products related to tricarboxylic acid cycle and Warburg metabolism being the most frequently reported.
Article
Microbiology
Evy Maas, John Penders, Koen Venema
Summary: Most research on the human microbiome has focused on bacteria, with less attention given to fungi and their role in human health, particularly their modulation through diet. This study used a validated in vitro model of the colon to investigate the gut fungal community and its response to different dietary interventions. The results showed that the mycobiome had lower diversity and greater variability compared to the bacterial community, and that diet could influence its composition and diversity.
Article
Microbiology
Evy Maas, John Penders, Koen Venema
Summary: This study investigated the relationships between fungi and bacteria using a complex computer-controlled in vitro model of the colon. The results showed that cross-kingdom interactions exist between fungi and bacteria, and they can influence each other. These findings are important for understanding the role of the microbial community in the human gut.
Article
Microbiology
Evy Maas, John Penders, Koen Venema
Summary: Most studies on the human gut microbiota focus on bacteria, but recent research indicates that intestinal fungi are also important for maintaining health. This study analyzed the mycobiome of healthy individuals and its interaction with the bacterial component of the microbiome. The results showed a lower diversity of fungi compared to bacteria, with significant inter-individual variation. Positive correlations were observed between certain fungi and bacteria, including those associated with alleviating inflammatory bowel disease. Further research is needed to investigate the importance of these correlations and distinguish between gut colonizers and transient species.
Article
Microbiology
Jacky Flipse, Anne-Marie Niekamp, Anne Dirks, Nicole H. T. M. Dukers-Muijrers, Christian J. P. A. Hoebe, Petra Wolffs, Inge H. M. van Loo
Summary: Treponema pallidum subsp. pallidum is the causative agent of syphilis, and its diagnosis and disease staging are based on clinical findings and serologic testing. PCR analysis of swab samples from genital ulcers is recommended, but its added value is debatable. In this study, both PCR and IgM serology were found to aid in the diagnosis of early syphilis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Tom Schoenmakers, Bas C. T. Van Bussel, Stefan H. M. Gorissen, Inge H. M. Van Loo, Frank Van Rosmalen, Wilhelmine P. H. G. Verboeket-van de Venne, Petra F. G. Wolffs, Walter N. K. A. Van Mook, Mathie P. G. Leers, CoLalC Consortium
Summary: This study aims to investigate whether biochemical and haematological changes due to the patient's host response (CoLab algorithm) in combination with a SARS-CoV-2 viability PCR (v-PCR) can be used to determine when a patient with COVID-19 is no longer infectious. The study consists of three parts using different cohorts of patients, aiming to develop an algorithm, investigate its efficacy, and validate it. The results will be disseminated through peer-reviewed journals and presentations.
Article
Cultural Studies
Alena Kamenshchikova, Petra F. G. Wolffs, Christian J. P. A. Hoebe, John Penders, Klasien Horstman
Summary: Complex phenomena, such as antimicrobial resistance (AMR), are often explained in biomedical sciences using analogies and metaphors. These metaphors play a crucial role in knowledge production and can shape scientific models of thought. Some metaphors, like 'AMR is an apocalypse' or 'antibiotics are weapons', are recognized immediately, but others, such as 'bacterial reservoirs' and 'bacterial colonies', are presented as literal and descriptive. However, these terms still carry historical links and can perpetuate thinking about bacteria as foreign actors.
SCIENCE AS CULTURE
(2023)
Article
Microbiology
Danyta I. Tedjo, Jennifer A. Wilbrink, Jos Boekhorst, Harro M. Timmerman, Simon W. Nienhuijs, Arnold Stronkhorst, Paul H. M. Savelkoul, Ad A. M. Masclee, John Penders, Daisy M. A. E. Jonkers
Summary: This study investigated the changes in fecal microbiota composition in obese individuals before and after sleeve gastrectomy (SG) and found a significant shift in microbiota composition after the surgery, but not towards a leaner profile. There were no correlations found between inflammatory markers, intestinal permeability, and changes in microbial profile.
