Article
Immunology
Rachel L. Markley, Katherine H. Restori, Bhuvana Katkere, Sarah E. Sumner, McKayla J. Nicol, Anastasia Tyryshkina, Shaneice K. Nettleford, David R. Williamson, David E. Place, Kalyan K. Dewan, Ashley E. Shay, Bradley A. Carlson, Santhosh Girirajan, K. Sandeep Prabhu, Girish S. Kirimanjeswara
Summary: This study showed that F. tularensis lacks the ability to utilize selenium effectively, while mice deficient in selenium are more susceptible to bacterial infections and have shorter survival times. Host selenoproteins found in macrophages were able to restrict bacterial replication, indicating a potential key role in combating bacterial infections.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Heloise Rytter, Anne Jamet, Jason Ziveri, Elodie Ramond, Mathieu Coureuil, Pauline Lagouge-Roussey, Daniel Euphrasie, Fabiola Tros, Nicolas Goudin, Cerina Chhuon, Ivan Nemazanyy, Fabricio Edgar de Moraes, Carlos Labate, Ida Chiara Guerrera, Alain Charbit
Summary: Metabolic pathways are crucial to the virulence of pathogenic bacteria and potential targets for antibacterial interventions. The pentose phosphate pathway (PPP) plays a key role in the intracellular survival of Francisella novicida, connecting with multiple other metabolic networks. Proteomics and metabolomics analyses show that PPP is a major hub linking glycolysis, the tricarboxylic acid cycle, fatty acid degradation, and sulfur metabolism, highlighting its importance in Francisella's pathogenesis and growth.
Letter
Immunology
Aurelie Hennebique, Yvan Caspar, Max Maurin, Sandrine Boisset, Isabelle Pelloux, Maria Pilar Gallego-Hernanz, Christophe Burucoa, France Cazenave-Roblot, Chloe Plouzeau, Blandine Rammaert
Summary: We report a case of human cutaneous infection with bacteremia caused by a Francisella species recently identified from seawater and fishes in an immunocompromised patient in France.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Cell Biology
Chui-Yoke Chin, Jinshi Zhao, Anna C. Llewellyn, Igor Golovliov, Anders Sjostedt, Pei Zhou, David S. Weiss
Summary: The study reveals the important role of an uncharacterized protein FlmX in the intracellular pathogen Francisella, affecting bacterial virulence and resistance to antibiotics by regulating LPS modification process. FlmX may represent a potential novel drug target for reducing bacterial virulence and increasing sensitivity to innate antimicrobials and antibiotics, with its conservation in other intracellular pathogens also noted.
Review
Microbiology
Klara Kubelkova, Ales Macela
Summary: This article summarizes the current knowledge on the innate and acquired humoral immune responses initiated by Francisella infection and their relationships with the immune defense systems.
Article
Microbiology
Brandi E. Hobbs, Courtney A. Matson, Vasileios Theofilou, Tonya J. Webb, Rania H. Younis, Eileen M. Barry
Summary: The FptA and FptF proteins of Ft are crucial for pathogenesis and modulation of host immune response, and LVS Delta fptA and LVS Delta fptF vaccine strains are highly attenuated and provide effective protection against lethal challenge.
Article
Biology
Ivo Fabrik, Orsolya Bilkei-Gorzo, Maria Oberg, Daniela Fabrikova, Johannes Fuchs, Carina Sihlbom, Melker Goransson, Anetta Hartlova
Summary: Resident tissue macrophages are specialized phagocytes responsible for maintaining tissue homeostasis. A quantitative proteomics approach identified cathepsins as key determinants of phagosome maturation in different tissues. Cathepsin K (CtsK) was found to be a molecular marker in lung phagosomes, required for intracellular protein and collagen degradation. Inhibition of CtsK activity reduced phagosomal proteolysis and collagenolysis in lung-resident macrophages. These findings highlight the importance of CtsK in lung phagosomes and its role in phagocytic collagen clearance in the lungs.
LIFE SCIENCE ALLIANCE
(2023)
Article
Chemistry, Medicinal
Heer H. Mehta, Xinhao Song, Yousif Shamoo
Summary: In vitro experimental evolution is a valuable tool to identify genetic changes responsible for antimicrobial resistance. However, the in vivo context contributes to the success of evolutionary trajectories, particularly in intracellular niches. This study conducted ex vivo evolution to identify antibiotic resistance in Francisella tularensis subsp. holarctica within macrophages, and found differences in mutational profiles between different environments. The results demonstrate the significance of host mediated stress during resistance evolution.
ACS INFECTIOUS DISEASES
(2023)
Article
Microbiology
Maha Alqahtani, Zhuo Ma, Jacob Miller, Jen Yu, Meenakshi Malik, Chandra Shekhar Bakshi
Summary: This study investigated the mechanisms of Aim2 inflammasome activation in macrophages infected with Francisella novicida and the Francisella tularensis live vaccine strain (LVS). The results showed that LVS-infected macrophages exhibited lower levels of Aim2-dependent IL-1 beta compared to novicida-infected macrophages, which was attributed to the suppression of the cGAS-STING DNA-sensing pathway. Additionally, damaged mitochondria-derived DNA might contribute to the robust Aim2-dependent IL-1 beta levels observed in novicida-infected macrophages.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Ivona Pavkova, Monika Kopeckova, Marek Link, Erik Vlcak, Vlada Filimonenko, Lenka Lecova, Jitka Zakova, Pavlina Laskova, Valeria Sheshko, Miloslav Machacek, Jiri Stulik
Summary: This study discovered the involvement of Francisella tularensis GAPDH homologue GapA in multiple pathogenic processes at the host cell level. The localization of GapA was demonstrated using immunoelectron microscopy, and its interaction with multiple host cellular proteins was identified using quantitative proteomic approaches. The impact of GapA deletion on cytokine gene transcription and signaling pathway activation was investigated. The results showed that GapA enhanced the transcription of various cytokines, which was not observed in cells infected with a fully virulent wild-type strain. This study provides evidence that GapA is directly involved in multiple host cellular processes and contributes to various molecular mechanisms of pathogenesis.
Article
Biochemical Research Methods
Jana Klimentova, Pavel Rehulka, Ivona Pavkova, Klara Kubelkova, Jan Bavlovic, Jiri Stulik
Summary: The study compares the proteomic composition of OMV and membrane fractions from two strains of Francisella tularensis, proposing a novel approach for cross-species proteomic comparison. By consecutively comparing the subproteomes of the two strains, differences in protein amounts caused by global expression changes can be distinguished from those caused by preferential protein packing into OMV or membranes.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Microbiology
Yinshi Yue, Bhanwar Lal Puniya, Tomas Helikar, Benjamin Girardo, Steven H. Hinrichs, Marilynn A. Larson
Summary: Francisella tularensis, a highly infectious zoonotic pathogen causing a deadly disease, exhibits major disparities in arginine catabolism and subsequent polyamine biosynthesis among its subpopulations, leading to differences in pathogenicity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Kendall Souder, Emma J. Beatty, Siena C. McGovern, Michael Whaby, Emily Young, Jacob Pancake, Daron Weekley, Justin Rice, Donald A. Primerano, James Denvir, Joseph Horzempa, Deanna M. Schmitt
Summary: The phenoxazine dye resazurin displays bactericidal activity against Francisella tularensis and Neisseria gonorrhoeae, with one derivative significantly reducing vaginal colonization by the latter in a mouse model. Through whole genome sequencing, mutations in genes dipA and pilD were identified in resazurin-resistant F. tularensis strains. Complementation of these genes partially restored sensitivity to resazurin, indicating their novel roles in susceptibility.
Article
Immunology
Roberto De Pascalis, Blake Frey, Helen M. Rice, Varunika Bhargava, Terry H. Wu, Ross L. Peterson, J. Wayne Conlan, Anders Sjostedt, Karen L. Elkins
Summary: This study evaluated a panel of live attenuated vaccines derived from SchuS4 to predict vaccine efficacy through in vitro measurements of T-cell functions and multivariate regression analyses, which were validated by rat vaccination and challenge studies. The results indicated that SchuS4-Delta clpB and Delta clpB-Delta capB may be better vaccine candidates than LVS.
Article
Microbiology
P. Todd Benziger, Erik J. Kopping, Patrick A. McLaughlin, David G. Thanassi
Summary: This study investigates the host apoptotic responses to F. tularensis and identifies the signaling pathways that are modulated by the bacteria to delay apoptosis and promote virulence. The findings reveal the mechanisms by which intracellular pathogens subvert host responses and enhance our understanding of the pathogenesis of tularemia.
Review
Cell Biology
Jean Celli
CELLULAR MICROBIOLOGY
(2015)
Article
Microbiology
Aria Eshraghi, Jungyun Kim, Alexandra C. Walls, Hannah E. Ledvina, Cheryl N. Miller, Kathryn M. Ramsey, John C. Whitney, Matthew C. Radey, S. Brook Peterson, Brittany R. Ruhland, Bao Q. Tran, Young Ah Goo, David R. Goodlett, Simon L. Dove, Jean Celli, David Veesler, Joseph D. Mougous
CELL HOST & MICROBE
(2016)
Review
Biochemistry & Molecular Biology
Cheryl Miller, Jean Celli
JOURNAL OF MOLECULAR BIOLOGY
(2016)
Article
Microbiology
Erin P. Smith, Cheryl N. Miller, Robert Child, Jennifer A. Cundiff, Jean Celli
Article
Microbiology
Leigh A. Knodler, Shauna M. Crowley, Ho Pan Sham, Hyungjun Yang, Marie Wrande, Caixia Ma, Robert K. Ernst, Olivia Steele-Mortimer, Jean Celli, Bruce A. Vallance
CELL HOST & MICROBE
(2014)
Article
Cell Biology
Elizabeth Di Russo Case, Audrey Chong, Tara D. Wehrly, Bryan Hansen, Robert Child, Seungmin Hwang, Herbert W. Virgin, Jean Celli
CELLULAR MICROBIOLOGY
(2014)
Article
Immunology
Gregory T. Robertson, Robert Child, Christine Ingle, Jean Celli, Michael V. Norgard
INFECTION AND IMMUNITY
(2013)
Article
Immunology
Gregory T. Robertson, Elizabeth Di Russo Case, Nicole Dobbs, Christine Ingle, Murat Balaban, Jean Celli, Michael V. Norgard
INFECTION AND IMMUNITY
(2014)
Review
Microbiology
Jean Celli, Renee M. Tsolis
NATURE REVIEWS MICROBIOLOGY
(2015)
Article
Multidisciplinary Sciences
Dedeke Rockx-Brouwer, Audrey Chong, Tara D. Wehrly, Robert Child, Deborah D. Crane, Jean Celli, Catharine M. Bosio
Article
Multidisciplinary Sciences
Audrey Chong, Robert Child, Tara D. Wehrly, Dedeke Rockx-Brouwer, Aiping Qin, Barbara J. Mann, Jean Celli
Article
Microbiology
Maarten F. de Jong, Tregei Starr, Maria G. Winter, Andreas B. den Hartigh, Robert Child, Leigh A. Knodler, Jan Maarten van Dijl, Jean Celli, Renee M. Tsolis
Article
Medicine, Research & Experimental
Jean Celli, Thomas C. Zahrt
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE
(2013)
Article
Microbiology
Sebenzile Myeni, Robert Child, Tony W. Ng, John J. Kupko, Tara D. Wehrly, Stephen F. Porcella, Leigh A. Knodler, Jean Celli
Article
Microbiology
Cheryl N. Miller, Erin P. Smith, Jennifer A. Cundiff, Leigh A. Knodler, Jessica Bailey Blackburn, Vladimir Lupashin, Jean Celli
CELL HOST & MICROBE
(2017)