Article
Microbiology
Andre Becker S. Saidenberg, Arnoud H. M. van Vliet, Marc Stegger, Thor Bech Johannesen, Torsten Semmler, Marcos Cunha, Alessandro C. de O. Silveira, Eleine Kuroki Anzai, Isabel C. A. Scaletsky, Anders Dalsgaard, Roberto M. La Ragione, Terezinha Knobl
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen associated with human and companion animal diseases, while avian pathogenic pathotype (APEC) severely impacts poultry health and production. The similarities between APEC and human ExPEC suggest a potential for zoonotic transmission.
VETERINARY MICROBIOLOGY
(2022)
Article
Immunology
Eveline Weerdenburg, Todd Davies, Brian Morrow, Aldert L. Zomer, Peter Hermans, Oscar Go, Bart Spiessens, Thijs van den Hoven, Gunter van Geet, Moussa Aitabi, Chitrita DebRoy, Edward G. Dudley, Marc Bonten, Jan Poolman, Jeroen Geurtsen
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a major cause of bacteremia worldwide, and the dominant O serotype among ExPEC bacteremia isolates is O25B, particularly among multidrug-resistant isolates.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Ying Yu, Bo Hu, Huanhuan Fan, Hailing Zhang, Shizhen Lian, Hongye Li, Shuangshuang Li, Xijun Yan, Shaohui Wang, Xue Bai
Summary: The study identified molecular epidemiology and biological characteristics of Escherichia coli associated with hemorrhagic pneumonia (HP) in mink from five Chinese provinces. Analysis included serotyping, antimicrobial susceptibility, detection of virulence genes, phylogenetic grouping, whole-genome sequencing, drug resistant gene, multilocus sequence typing (MLST), and biofilm formation assays.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Junxin Zhou, Weiping Wang, Min Liang, Qian Yu, Shiqi Cai, Tailong Lei, Yan Jiang, Xiaoxing Du, Zhihui Zhou, Yunsong Yu
Summary: The study aimed to investigate the molecular mechanism underlying ceftazidime-avibactam resistance caused by a novel CMY-178 variant. The results showed that CMY-178 increased the resistance to multiple cephalosporins and ceftazidime-avibactam compared with CMY-172. Structural modeling and molecular docking revealed that CMY-178 had a narrower ligand-binding pocket and a stronger positive charge at the pocket entrance than CMY-172. The bla(CMY-178) gene was located in a transferable plasmid with high transfer frequency and no fitness costs.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Jie Yang, Gang Xiao, Ning Xiao, Zonghan Jiang, Chao Jiang, Yujuan Li, Wenxin Chen, Hongguang Lin, Zhiliang Sun, Jiyun Li
Summary: The plasmid-mediated tigecycline resistance gene tet(X4) was found in Escherichia coli isolates from chicken and pig farms in Hunan province, China. Among the 257 E. coli samples, six tested positive for tet(X4). These tet(X4)-positive strains showed high resistance to multiple drugs, including tigecycline. Whole-genome sequencing and bioinformatics analysis revealed the genomic locations of tet(X4) and identified the plasmid replicon types carrying this gene.
Review
Infectious Diseases
Johann D. D. Pitout, Liang Chen
Summary: Epidemic IncF plasmids have played a crucial role in the selective advantage of multidrug-resistant extraintestinal pathogenic Escherichia coli (ExPEC). These plasmids offer advantages to their hosts and have coevolved with bacterial host genomes, contributing to the success of ExPEC. IncF plasmids are large and diverse, often carrying antimicrobial resistance (AMR) and virulence associated factor (VAF) genes. They have become fixed in certain MDR ExPEC clones, such as ST131 and ST410, due to the presence of AMR, VAF genes, and other traits. Different subclades of ST131 and ST410 have emerged with different AMR genes on IncF platforms over time. The continuous use of antimicrobial agents increases the risk of the emergence of MDR ExPEC clone/IncF plasmid combinations.
INFECTIOUS DISEASES AND THERAPY
(2023)
Article
Medicine, General & Internal
Ji-Na Gu, Lin Chen, Xing-Bei Weng, Xiao-Yan Yang, Dan-Mei Pan
Summary: The research revealed that Escherichia coli Huamei202001 belongs to sequence type 410 and carries a blaNDM-5-encoding IncX3-type plasmid responsible for carbapenem resistance spreading. Only the patient was detected as having a positive faecal sample screening test for CRE.
FRONTIERS IN MEDICINE
(2021)
Review
Environmental Sciences
Shaqiu Zhang, Muhammad Abbas, Mujeeb Ur Rehman, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Dekang Zhu, Xinxin Zhao, Qun Gao, Bin Tian, Anchun Cheng
Summary: Colistin drug resistance is an emerging public health threat worldwide, with prevalence in multiple reservoirs and ecological environmental settings. Escherichia coli carrying mobile colistin resistance genes exhibit abundant clinically important resistance genes and are spread through various plasmid replicon types.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Piotr Majewski, Anna Gutowska, David G. E. Smith, Tomasz Hauschild, Paulina Majewska, Tomasz Hryszko, Dominika Gizycka, Boguslaw Kedra, Jan Kochanowicz, Jerzy Glowinski, Justyna Drewnowska, Izabela Swiecicka, Pawel T. Sacha, Piotr Wieczorek, Dominika Iwaniuk, Anetta Sulewska, Radoslaw Charkiewicz, Katarzyna Makarewicz, Agnieszka Zebrowska, Slawomir Czaban, Piotr Radziwon, Jacek Niklinski, Elzbieta A. Tryniszewska
Summary: The study identified six extraintestinal Escherichia coli isolates that produce the mcr-1.1 gene, leading to colistin resistance. These strains also possess multiple virulence factors. The IncX4 plasmids carrying mcr genes were found to be disseminated among E. coli isolates and may facilitate the spread of colistin resistance genes between different clones.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Liyun Liu, Ling Zhang, Haijian Zhou, Min Yuan, Dalong Hu, Yonglu Wang, Hui Sun, Jianguo Xu, Ruiting Lan
Summary: This prospective study investigated molecular characteristics and antimicrobial susceptibility patterns of Citrobacter spp. isolates from extraintestinal infections. The results showed a diverse range of Citrobacter isolates causing extraintestinal infections and a high prevalence of multidrug resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Mackenzie Dorr, Aryeh Silver, Dylan Smurlick, Ananta Arukha, Subhashinie Kariyawasam, Adelumola Oladeinde, Kimberly Cook, Thomas Denagamage
Summary: This study sequenced and annotated three plasmids carrying the bla(CTX-M-1) gene, and confirmed their transferability between different serovars and species of bacteria. The transfer of plasmids was successful, with varying frequencies depending on the donor strain.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Multidisciplinary Sciences
Sirirat Luk-in, Tanittha Chatsuwan, Naris Kueakulpattana, Ubolrat Rirerm, Dhammika Leshan Wannigama, Rongpong Plongla, Ratana Lawung, Chaiwat Pulsrikarn, Siriporn Chantaroj, Pattharaporn Chaichana, Nattida Saksaengsopa, Teerarat Shanthachol, Malee Techapornroong, Sunee Chayangsu, Wanla Kulwichit
Summary: This study reported a high rate of mcr-carrying Salmonella clinical isolates in Thailand, associated with mcr-3 gene and bla(CTX-M-55) co-existence in S. Choleraesuis for the first time, leading to clinical concern. The study highlights the potential reservoirs and vectors in the dissemination of mcr and the co-selection by colistin and/or cephalosporins.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Serena Simoni, Marina Mingoia, Andrea Brenciani, Maria Carelli, Maria M. Lleo, Giovanni Malerba, Carla Vignaroli
Summary: The coexistence of carbapenem and colistin resistance genes in a single plasmid presents a significant concern for future clinical therapies.
Article
Infectious Diseases
Isabella F. S. Figueiredo, Lorena G. Araujo, Raissa G. Assuncao, Itaynara L. Dutra, Johnny R. Nascimento, Fabricia S. Rego, Carolina S. Rolim, Leylane S. R. Alves, Mariana A. Frazao, Samilly F. Cadete, Luis Claudio N. da Silva, Joicy C. de Sa, Eduardo M. de Sousa, Waldir P. Elias, Flavia R. F. Nascimento, Afonso G. Abreu
Summary: The study showed that cinnamaldehyde has potential in treating sepsis by reducing inflammation, increasing cell production in bone marrow and spleen, and improving survival rates in animals.
Article
Ecology
Ayush Pathak, Daniel C. Angst, Ricardo Leon-Sampedro, Alex R. Hall
Summary: Some bacterial resistance mechanisms can degrade antibiotics, potentially protecting neighboring susceptible cells. However, the effects of such mechanisms on bacterial communities of more than two species are not well understood. By conducting experiments on multispecies communities, we found that resistance in one species reduced antibiotic inhibition of other species, but the extent of benefit varied among species.
Article
Biotechnology & Applied Microbiology
E. Gingerich, T. Frana, C. M. Logue, D. P. Smith, H. O. Pavlidis, W. E. Chaney
Summary: The study showed that adding SCFP to the diet can significantly reduce the amount of Salmonella Enteritidis in the ceca of young layer pullets, which could improve the food safety level of commercial laying hens.
JOURNAL OF FOOD PROTECTION
(2021)
Article
Chemistry, Multidisciplinary
Lara L. Reys, Vijayaganapathy Vaithilingam, Mireille M. J. P. E. Sthijns, Eduardo Soares, Timo Rademakers, Rick de Vries, Sami G. Mohammed, Denise de Bont, Marlon J. Jetten, Carolin Hermanns, Omar Paulino da Silva Filho, Adam Stell, Nizar I. Mourad, Pierre Gianello, Vanessa L. S. LaPointe, Simone S. Silva, Rui L. Reis, Tiago H. Silva, Aart A. van Apeldoorn
Summary: Fucoidan, derived from algae, has antioxidant properties and can improve the viability and function of microencapsulated beta cells. When incorporated in ultrapure alginate, fucoidan significantly increases the survival rate and glucose responsiveness of islets, providing a redox-modulatory niche and immune barrier.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Microbiology
Sathya N. Kulappu Arachchige, Anna Kanci Condello, Ling Zhu, Pollob K. Shil, Kelly A. Tivendale, Gregory J. Underwood, Amir H. Noormohammadi, Glenn F. Browning, Nadeeka K. Wawegama
Summary: Immunosuppression induced by CAV or IBDV infection can interfere with the efficacy of vaccination against Mycoplasma gallisepticum in chickens, affecting the level of protection and immune response against the pathogen.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Aline L. de Oliveira, Timothy J. Johnson, Nicolle L. Barbieri, Lisa K. Nolan, Catherine M. Logue
Summary: The genome sequence of NMEC15, a strain of neonatal meningitis Escherichia coli belonging to serotype O18:K1, isolated from the cerebrospinal fluid of an infant with neonatal bacterial meningitis in the Netherlands, is reported here.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Biochemistry & Molecular Biology
Catherine A. Chambers, Alexis S. Dadelahi, Charles R. Moley, Rachel M. Olson, Catherine M. Logue, Jerod A. Skyberg
Summary: This study investigated the role of NMEC strain-dependent P2X receptor (P2XR) signaling on the outcome of infection in neonatal mice. The results showed that alpha-hemolysin (HlyA)-expressing NMEC (HlyA(+)) induced robust P2XR-dependent macrophage cell death and P2XR inhibition increased mortality in neonatal mice infected with HlyA(+) NMEC. The findings also suggested that P2XR-dependent protection against HlyA(+) NMEC required macrophages.
Editorial Material
Microbiology
Byeonghwa Jeon, Taradon Luangtongkum, Zhangqi Shen, Catherine M. Logue, Jun Lin
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Aline L. de Oliveira, Nicolle L. Barbieri, Darby M. Newman, Meaghan M. Young, Lisa K. Nolan, Catherine M. Logue
Summary: Avian pathogenic E. coli is the causative agent of extra-intestinal infections in birds known as colibacillosis. The T6SS genes were significantly more prevalent in APEC and may be involved in the virulence of APECO18.
Article
Multidisciplinary Sciences
Meaghan M. Young, Aline L. de Oliveira, Lisa K. Nolan, Nicolle L. Barbieri, Catherine M. Logue
Summary: This study aimed to investigate biofilm formation and related genes in avian pathogenic Escherichia coli (APEC). Through mutant screening and PCR analysis, nine genes associated with biofilm formation in APEC were identified, and four mutant genes showed significantly decreased biofilm formation ability. These findings are important for further understanding the mechanisms of APEC biofilm formation.
Article
Immunology
Huiwen Wang, Liu Cao, Catherine M. Logue, Nicolle L. Barbieri, Lisa K. Nolan, Jun Lin
Summary: Colibacillosis is a common and devastating infectious disease in poultry production. Universal vaccines are urgently needed to protect chickens from avian pathogenic Escherichia coli (APEC) infections. A novel Ent conjugate vaccine induced Ent-specific antibodies that inhibited the growth of diverse APEC strains.
Article
Veterinary Sciences
Roel Becerra, Jenny Nicholds, Karen Grogan, David French, Eric Shepherd, Catherine M. Logue
Summary: Spotty liver disease (SLD) has become an important cause of disease in egg-producing flocks, with organisms such as Campylobacter hepaticus and Campylobacter bilis causing liver lesions and negative impacts on egg production and mortality of valuable hens.
Article
Microbiology
Roel Becerra, Julia Ienes-Lima, Jenny Nicholds, Catherine M. Logue
Summary: Spotty liver disease (SLD) is a significant cause of disease in egg-producing flocks, with Campylobacter hepaticus and Campylobacter bilis being the implicated organisms. We report the complete genome sequence of C. hepaticus strain RBCL71delta and three additional strains isolated from a layer flock in Georgia, USA.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Julia Ienes-Lima, Roel Becerra, Catherine M. M. Logue
Summary: Campylobacter hepaticus has re-emerged as a significant cause of disease in egg laying birds worldwide, leading to morbidity, mortality, and egg losses in the poultry industry. The disease is characterized by liver spots and histopathological analysis reveals necrotizing hepatitis. The re-emergence of C. hepaticus may be linked with housing practices and colonization in pasture raised birds.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Huiwen Wang, Catherine M. Logue, Lisa K. Nolan, Jun Lin
Summary: Colibacillosis, caused by avian pathogenic Escherichia coli (APEC), is a significant infectious disease in chickens and protecting young chickens from this disease is important. A novel enterobactin (Ent) conjugate vaccine was developed to induce high levels of anti-Ent immunoglobulin Y (IgY) in chicken serum, but the maternal immunity from the vaccinated hens did not provide significant protection against APEC challenge in the young chicks. Therefore, further optimization of the APEC infection model and improvement of the maternal immunization regimen are needed.
Article
Veterinary Sciences
Yu-Yang Tsai, Monique Franca, Alvin Camus, Lisa J. Stabler, Nicolle Barbieri, Catherine M. Logue
Summary: This study aimed to identify the bacteria commonly found in focal duodenal necrosis (FDN) lesions in table egg layers using histopathological analysis. The results showed no consistent bacterial species identified in FDN samples. However, analysis of relative phylum abundance revealed differences in the duodenal microbiota between FDN-positive and FDN-negative control samples, indicating intestinal dysbiosis. Bacteriological analysis of FDN lesions further identified Escherichia coli as a commonly present Gram-negative bacterium with potential pathogenic virulence genes associated with intestinal disease.
Article
Food Science & Technology
Phillip Brown, Yi Chen, Robin Siletzky, Cameron Parsons, Lee-Ann Jaykus, Joseph Eifert, Elliot Ryser, Catherine M. Logue, Christina Stam, Eric Brown, Sophia Kathariou
Summary: Listeria monocytogenes, a Gram-positive foodborne pathogen, has the ability to persist in food processing plants and contaminate ready-to-eat foods. A study investigating 54 L. monocytogenes strains from four turkey processing plants in the US found that allelic profiles across the whole genome can be specific to individual processing plants. Whole genome sequencing data has the potential to identify facility-specific signatures for tracking strain movement and environmental surveillance in food processing facilities.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2021)