期刊
ADVANCED FUNCTIONAL MATERIALS
卷 31, 期 35, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.202011205
关键词
beta-cells; alginate; antioxidant; diabetes type I; fucoidan; microcapsules; pancreatic cells
类别
资金
- Portuguese FCT [SFRH/BD/112139/2015, PTDC/CTM-CTM//29813/2017]
- Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement [NORTE-01-0145-FEDER-000021, NORTE-01-0145-FEDER-000023]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/112139/2015, PTDC/CTM-CTM/29813/2017] Funding Source: FCT
Fucoidan, derived from algae, has antioxidant properties and can improve the viability and function of microencapsulated beta cells. When incorporated in ultrapure alginate, fucoidan significantly increases the survival rate and glucose responsiveness of islets, providing a redox-modulatory niche and immune barrier.
Microencapsulating pancreatic islets in immunoprotective alginate hydrogels is a promising strategy for treatment of type 1 diabetes. However, this strategy is limited by inflammation and hypoxia mediated oxidative stress, due to encapsulation and the hydrogel itself, leading to impaired insulin secretion and limited short and long term cell survival. Herein, the antioxidant effect of fucoidan, an algae derived polysaccharide, on beta cells, and its positive effects on encapsulated beta cell viability and function is presented. Fucoidan from Fucus vesiculosus (FF) exhibits a high total antioxidant capacity, and free radical scavenging activity, and is able to significantly alleviate intracellular oxidative stress in rat insolinoma beta cells (INS1E). In addition, FF significantly increases insulin secretion in a dose- and time-dependent manner. When FF is incorporated in ultrapure alginate used for microencapsulation of primary rat islets, both viability and glucose responsiveness of rat islets in these socalled Fucogel microcapsules (Fucocaps) are found to be significantly higher compared to islets encapsulated in alginate alone. Similar results are obtained with INS1E pseudoislets and neonatal pig islets. Fucocaps can provide a redox-modulatory niche and an immune barrier for islets and beta cells in the same time leading to significantly improved survival and endocrine function by mitigating oxidative strees.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据