Article
Agriculture, Dairy & Animal Science
Jian Tu, Dandan Fu, Yi Gu, Ying Shao, Xiangjun Song, Mei Xue, Kezong Qi
Summary: APEC is the responsible pathogen for colibacillosis in poultry, and the role of YgeK in APEC has not been reported. This study found that YgeK plays a crucial role in APEC virulence by affecting flagellar formation, bacterial motility, serum sensitivity, and adhesion ability. The results contribute to understanding the function of the ETT2 cluster in APEC and clarify its pathogenic mechanism.
Article
Virology
Kat R. Smith, Emmanuel W. Bumunang, Jared Schlechte, Matthew Waldner, Hany Anany, Matthew Walker, Kellie MacLean, Kim Stanford, John M. Fairbrother, Trevor W. Alexander, Tim A. Mcallister, Mohamed Faizal Abdul-Careem, Yan D. Niu
Summary: In this study, bacteriophages with the potential to control colibacillosis in chickens were isolated from hen feces and human sewage. These phages showed broad host range, pH tolerance, and no genes associated with virulence or antimicrobial resistance were detected. Comparative genomic analysis and phylogenetic trees revealed the classification and genetic relatedness of the phages. The findings suggest that diverse APEC-infecting phages in the environment could be used for controlling colibacillosis in poultry.
Article
Microbiology
Yue Han, Ping Luo, Yuji Chen, Jiali Xu, Jing Sun, Chiyu Guan, Pu Wang, Mianmian Chen, Xian Zhang, Yueyue Zhu, Tingting Zhu, Ruidong Zhai, Changyong Cheng, Houhui Song
Summary: This study demonstrates the development of an innovative recombinant Salmonella strain with regulated heterologous O-antigen expression, which can stimulate robust antibody responses and provide stronger protection against APEC O-78 and S. Typhimurium infections.
VETERINARY MICROBIOLOGY
(2021)
Article
Infectious Diseases
Muhammad Usman, Muhammad Hidayat Rasool, Mohsin Khurshid, Bilal Aslam, Zulqarnain Baloch
Summary: This study aimed to detect carbapenem-resistant co-harboring mcr-1 avian pathogenic E. coli (APEC) in broiler chickens infected with colibacillosis using molecular techniques. Results showed that 37% of the isolates were confirmed as APEC, with 8.4% found to be carbapenem-resistant APEC, and 38% of the carbapenem-resistant APEC co-harbored the mcr-1 gene. Improper use of antibiotics in poultry production systems is contributing to the emergence of CR-APEC co-harboring the mcr-1 gene.
Article
Microbiology
Xuhua Chen, Wenxing Liu, Huoming Li, Shigan Yan, Fengwei Jiang, Wentong Cai, Ganwu Li
Summary: Avian pathogenic Escherichia coli (APEC) show great genetic diversity with various virulence-associated genes that could serve as markers. Different virulence-associated gene clusters are significantly associated with specific phylogroups of APEC, suggesting a distinct distribution pattern. This study provides insights into the evolution and pathogenesis of APEC, potentially leading to the development of new diagnostic tools.
VETERINARY MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Lei Yin, Baoyan Cheng, Jian Tu, Ying Shao, Xiangjun Song, Xiaocheng Pan, Kezong Qi
Summary: This study investigates the role of transcriptional regulator YqeH in the pathogenicity of avian pathogenic Escherichia coli (APEC). The results show that the deletion of YqeH leads to reduced motility and biofilm formation, resulting in decreased virulence of APEC. Additionally, YqeH regulates the transcriptional levels of genes associated with flagellum, biofilm, and virulence, impacting the pathogenicity of APEC.
VETERINARY RESEARCH
(2022)
Article
Microbiology
Zhuohao Wang, Xiangkuan Zheng, Genglin Guo, Zimeng Hu, Jinfeng Miao, Yongyi Dong, Zhengjun Xu, Qingan Zhou, Xiankai Wei, Xiangan Han, Yuqing Liu, Wei Zhang
Summary: Among Avian pathogenic Escherichia coli (APEC) serogroups in China, O145 may be emerging as a predominant serogroup. This serogroup has been overlooked but may have stronger pathogenicity. Genomic analysis revealed possible reasons for its prevalence. This newly predominant serogroup should be given more attention, not limited to the three traditional APEC serogroups.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Camille Lucas, Sabine Delannoy, Catherine Schouler, Rozenn Souillard, Laetitia Le Devendec, Pierrick Lucas, Alassane Keita, Patrick Fach, Julie Puterflam, Stephanie Bougeard, Isabelle Kempf
Summary: This study identified relevant genetic markers associated with avian pathogenic E. coli (APEC) using high-throughput PCR and a machine learning procedure, which is crucial for better control of avian colibacillosis.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Dezhi Li, Wei Liang, Qingyue Hu, Jianluan Ren, Feng Xue, Qing Liu, Fang Tang
Summary: This study investigated the role of spontaneous induction prophages in avian pathogenic Escherichia coli (APEC) and found that phi458 promoted biofilm formation and decreased virulence by repressing virulence genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Courtney A. Fancher, Hudson T. Thames, Mary Gates Colvin, Mercedes Smith, Alyssa Easterling, Nikhil Nuthalapati, Li Zhang, Aaron Kiess, Thu T. N. Dinh, Anuraj Theradiyil Sukumaran
Summary: The study investigated the prevalence and virulence of E. coli in antibiotic-free broiler farms, determining that there is a high prevalence of virulent E. coli, especially during the spring season. Antimicrobial resistance was highest for tetracycline, and serogroups O8 and O78 were most frequent in virulent E. coli.
MICROBIOLOGY SPECTRUM
(2021)
Article
Veterinary Sciences
Dosseh Jean Apotre Afayibo, Hong Zhu, Beibei Zhang, Lan Yao, Hosny Ahmed Abdelgawad, Mingxing Tian, Jingjing Qi, Yali Liu, Shaohui Wang
Summary: This study identified the serotypes, phylogenetic groups, virulence genes distribution, and antibiotic resistance profiles of APEC isolates in eastern China. The APEC isolates were found to carry a variety of virulence genes and exhibit multi-antibiotic resistance profiles.
VETERINARY SCIENCES
(2022)
Article
Infectious Diseases
Samina Ievy, M. Nazmul Hoque, Md Saiful Islam, Md Abdus Sobur, Fatimah Muhammad Ballah, M. Shaminur Rahman, Md Bahanur Rahman, Jayedul Hassan, Mohammad Ferdousur Rahman Khan, M. D. Tanvir Rahman
Summary: This study sequenced the whole genome of APEC MTR_BAU02 strain isolated from layer birds and identified widespread antimicrobial resistance genes and virulence factor genes, similar to those found in human isolates, highlighting the growing threat of antimicrobial resistance in both poultry and humans.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Review
Biochemistry & Molecular Biology
Babak Pakbin, Wolfram M. Bruck, John W. A. Rossen
Summary: Escherichia coli are versatile microorganisms found in the normal intestinal microbiota of humans and animals. They can become emerging human pathogens by acquiring genes encoding virulence factors. Nine enteric E. coli pathotypes have been characterized, utilizing various virulence factors to cause intestinal and extraintestinal diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Ethan R. Wyrsch, Rhys N. Bushell, Marc S. Marenda, Glenn F. Browning, Steven P. Djordjevic
Summary: Lower urinary tract, renal, and bloodstream infections caused by phylogroup B2 extraintestinal pathogenic Escherichia coli (ExPEC) are a leading cause of morbidity and mortality. ST1193 is a multidrug-resistant sequence type of B2 phylogroup E. coli that is associated with various human infections. This study analyzed the virulence plasmids carried by ST1193 and found that they are similar to pUTI89, a key F virulence plasmid found in dominant extraintestinal pathogenic E. coli lineages. The findings of this study have significant implications for the epidemiological source tracking of ExPEC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Lei Yin, Qianwen Li, Zeping Wang, Jian Tu, Ying Shao, Xiangjun Song, Xiaocheng Pan, Kezong Qi
Summary: The E. coli type III secretion system 2 (ETT2) plays a crucial role in the virulence of avian pathogenic E. coli (APEC), with ygeG identified as a chaperone protein that affects biofilm formation, cell adherence, invasion, and bacterial virulence. Deletion of ygeG also enhances bacterial virulence in chicken infection models, regulating genes involved in biofilm formation, outer membrane proteins, fimbrial genes, and virulence effector proteins.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Public, Environmental & Occupational Health
M. Mellata, J. R. Johnson, R. Curtiss
ZOONOSES AND PUBLIC HEALTH
(2018)
Review
Food Science & Technology
Zachary R. Stromberg, Graham A. J. Redweik, Melha Mellata
FOODBORNE PATHOGENS AND DISEASE
(2018)
Article
Cell Biology
Zachary R. Stromberg, Angelica Van Goor, Graham A. J. Redweik, Meghan J. Wymore Brand, Michael J. Wannemuehler, Melha Mellata
DISEASE MODELS & MECHANISMS
(2018)
Article
Agriculture, Dairy & Animal Science
Graham A. J. Redweik, Zachary R. Stromberg, Angelica Van Goor, Melha Mellata
Article
Microbiology
Graham A. J. Redweik, Karrie Daniels, Andrew J. Severin, Mark Lyte, Melha Mellata
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Logan C. Ott, Zachary R. Stromberg, Graham A. J. Redweik, Michael J. Wannemuehler, Melha Mellata
Article
Agriculture, Dairy & Animal Science
Angelica Van Goor, Graham A. J. Redweik, Zachary R. Stromberg, Caroline G. Treadwell, Hongwei Xin, Melha Mellata
Article
Veterinary Sciences
Graham A. J. Redweik, Michael H. Kogut, Ryan J. Arsenault, Melha Mellata
FRONTIERS IN VETERINARY SCIENCE
(2020)
Article
Microbiology
Graham A. J. Redweik, Mary Kate Horak, Ryley Hoven, Logan Ott, Melha Mellata
Summary: The study found that treating young chickens with probiotics and live vaccines can reduce virulent E. coli plasmids in the intestines, leading to decreased levels of smRNA and potentially reduced siderophore production and antibiotic resistance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jared M. Jochum, Graham A. J. Redweik, Logan C. Ott, Melha Mellata
Summary: The study identified antimicrobial resistant bacteria in commercial poultry farms, including resistance to last-resort antibiotics. These resistant bacteria were found to be potentially transferable among different bacterial strains. The presence of multidrug resistant bacteria in both conventional cage and cage-free poultry indicates the need for further research to develop strategies for mitigation.
Article
Biotechnology & Applied Microbiology
Logan C. Ott, Holly J. Appleton, Hu Shi, Kevin Keener, Melha Mellata
Summary: The study demonstrates the efficiency of high voltage atmospheric cold plasma (HVACP) technology for deactivating fungal spores and toxins. Treated spores showed surface ablation and the DON toxin structure was significantly reduced.
Article
Microbiology
Logan C. Ott, Mark Engelken, Sara M. Scott, Elizabeth M. McNeill, Melha Mellata
Summary: This study demonstrates the use of Drosophila as a model for gut-mediated plasmid transfer. Host sex significantly influences the concentrations of donors and recipients in the gut, while transconjugant concentrations are not affected by host sex or genetics and were detected only in specific plasmid groups.
Article
Biology
Graham A. J. Redweik, Michael H. Kogut, Ryan J. Arsenault, Mark Lyte, Melha Mellata
Summary: The study demonstrates that reserpine enhances Salmonella resistance in the chicken intestine by modulating neuro-immunometabolic signaling, leading to reduced bacterial colonization and improved food safety.
COMMUNICATIONS BIOLOGY
(2021)
Review
Veterinary Sciences
Graham A. J. Redweik, Jared Jochum, Melha Mellata
FRONTIERS IN VETERINARY SCIENCE
(2020)
Article
Veterinary Sciences
Zachary R. Stromberg, Angelica Van Goor, Graham A. J. Redweik, Melha Mellata
FRONTIERS IN VETERINARY SCIENCE
(2018)