Article
Medicine, Research & Experimental
Marianne R. Spalinger, Vinicius Canale, Anica Becerra, Ali Shawki, Meli'sa Crawford, Alina N. Santos, Pritha Chatterjee, Jiang Li, Meera G. Nair, Declan F. McCole
Summary: Macrophages deficient in PTPN2 promote a strong IL-22-driven immune response, leading to accelerated disease but faster clearance of Citrobacter rodentium infection. Deletion of PTPN2 in epithelial cells results in a failure to upregulate antimicrobial peptides and eliminate the infection. Macrophage-derived IL-22 plays a crucial role in the induction of protective immune responses in the intestinal epithelium.
Article
Medicine, Research & Experimental
Meena Easwaran, Chloe Santa Maria, Joshua D. Martinez, Barbara Hung, Xuan Yu, Joanne Soo, Akari Kimura, Eric R. Gross, Elizabeth Erickson-DiRenzo
Summary: This study examines the short-term effects of e-cigarette inhalation on the mouse larynx and finds minimal cellular and inflammatory changes. Flavor and region-specific changes were observed after long-term exposure.
Article
Allergy
Satoshi Nunomura, Isao Kitajima, Yasuhiro Nanri, Midori Kitajima, Naoko Ejiri, I-Shuan Lai, Naoko Okada, Kenji Izuhara
Summary: The FADS mouse model, generated by specifically deleting Ikk2 in facial skin fibroblasts, exhibits similar ocular lesions to AKC in patients and can be used to examine the therapeutic effects of anti-AKC agents. Tear periostin, a novel biomarker for allergic conjunctival disease, is valuable for evaluating the efficacy of such treatments.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Changpei Gan, Jing Wang, Yaogeng Wang, Alejandra Martinez-Chavez, Michel Hillebrand, Niels de Vries, Joke Beukers, Maria C. Lebre, Els Wagenaar, Hilde Rosing, Sjoerd Klarenbeek, Onno B. Bleijerveld, Ji-Ying Song, Maarten Altelaar, Jos H. Beijnen, Alfred H. Schinkel
Summary: Mammalian carboxylesterase 1 (Ces1) enzymes play a vital role in metabolism, hydrolyzing numerous xenobiotics and endogenous lipids. This study identified a mouse strain lacking three Ces1 genes (Ces1c, Ces1d, and Ces1e), and demonstrated that this deficiency impaired drug and lipid metabolism, leading to altered body weight, inflammation, and lipid accumulation in adipose tissue and liver. These findings emphasize the importance of Ces1 enzymes in maintaining metabolic homeostasis and suggest their potential role in obesity-related chronic inflammation and metabolic diseases.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Microbiology
Arpita Aditya, Shaik O. Rahaman, Debabrata Biswas
Summary: This study demonstrated that metabolites from Lactobacillus casei can inhibit the growth of enterohemorrhagic E. coli and promote the growth of beneficial bacteria in a simulated rumen system, indicating a potential method for controlling the spread of cattle-borne pathogens like EHEC.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Article
Multidisciplinary Sciences
Alexa Canchola, Ruth Meletz, Riste Ara Khandakar, Megan Woods, Ying-Hsuan Lin
Summary: The toxicity mechanisms of VEA vaping are still not fully understood, as thermal degradation during the vaping process can produce various degradation products that may be more toxic than unvaped VEA. The impact of customizable parameters and heating coil temperature on vaping emissions has not been fully assessed.
Article
Biochemistry & Molecular Biology
Kentaro Akiyama, Kyaw Thu Aung, Laura Talamini, Olivier Huck, Takuo Kuboki, Sylviane Muller
Summary: Autophagy and its anomalies are potential targets for improving periodontal treatment outcomes. The phosphopeptide P140 has shown effectiveness in correcting autophagy dysfunctions and reducing inflammation in experimental periodontitis. Moreover, P140 contributes to bone preservation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Immunology
Bert Malengier-Devlies, Tatjana Decaesteker, Kaat Dekoster, Arno Vanstapel, Kourosh Ahmadzadeh, Fariba Poosti, Tania Mitera, Laura Seldeslachts, Erik Verbeken, Carine Wouters, Greetje Vande Velde, Jeroen Vanoirbeek, Patrick Matthys
Summary: Systemic juvenile idiopathic arthritis (sJIA) is an immune disorder with increasing reports of lung involvement in patients. A mouse model for sJIA showed lung abnormalities distinct from those seen in patients, indicating the importance of monitoring lung disease during systemic inflammation. The model provides a tool to explore the underlying mechanisms of lung pathology in an autoinflammatory disease context.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Kosuke Nishi, Takako Ito, Ayumu Kadota, Momoko Ishida, Hisashi Nishiwaki, Naohiro Fukuda, Naoaki Kanamoto, Yoko Nagata, Takuya Sugahara
Summary: The study showed that Citrus unshiu leaf extract has anti-inflammatory properties by inhibiting proinflammatory cytokines and chemokine secretion from macrophages. Oral administration of the extract also reduced proinflammatory cytokines levels and increased anti-inflammatory cytokine levels in a mouse model of systemic inflammation. Additionally, the extract decreased the secretion and gene expression of proinflammatory proteins in the liver and spleen of the model mice.
Article
Biochemistry & Molecular Biology
Shanshan Xu, Nan Wang, Dandan Yan, Yingjie Zhong
Summary: The study found that PE can alleviate asthma caused by obesity. In both mouse and cell experiments, PE can reduce airway inflammation and oxidative damage, as well as reduce body weight and inflammation levels in obese asthmatic mice.
MOLECULAR IMMUNOLOGY
(2023)
Article
Food Science & Technology
Haixin Li, Xinqiang Xie, Ying Li, Moutong Chen, Liang Xue, Juan Wang, Jumei Zhang, Shi Wu, Qinghua Ye, Shuhong Zhang, Runshi Yang, Hui Zhao, Lei Wu, Tingting Liang, Yu Ding, Qingping Wu
Summary: The study demonstrated that P. pentosaceus IM96 has protective effects against EHEC O157:H7 infection in female mice by reducing intestinal inflammation, enhancing intestinal barrier function, and modulating intestinal microbiota composition.
Article
Biology
Blagovesta Boneva, Nikola Ralchev, Petya Ganova, Andrey Tchorbanov, Nikolina Mihaylova
Summary: This study investigated the role of different types of immune cells in the development of osteoarthritis, finding infiltration of B lymphocytes and effector T cells, activation of cytotoxic T cells and NK cell populations, and differentiation of NK cells from a cytotoxic phenotype to an effector phenotype in different stages of the disease.
Article
Plant Sciences
Lethicia Souza Tavares, Betty Dorvigny Mancebo, Lucas Nunes Santana, Alluanan Adelson do Nascimento Silva, Roberta Lane de Oliveira Silva, Ana Maria Benko-Iseppon, Marcio Viana Ramos, Camila Tauane Monteiro do Nascimento, Thalles Barbosa Grangeiro, Jeanlex Soares Sousa, Rinaldo Aparecido Mota, Valdemiro Amaro da Silva Junior, Jose Vitor Lima-Filho
Summary: This study demonstrates that recombinant osmotin inclusion bodies from Calotropis procera can prevent infection-induced inflammation, providing a new approach for the treatment of infections.
Article
Plant Sciences
Adam Chin-Fatt, Rima Menassa
Summary: Chimeric fusion proteins consisting of VHH and Fc fragments show increased accumulation when targeted to the chloroplast thylakoid lumen, indicating the potential of this compartment for stable folding of disulfide-containing recombinant proteins.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Immunology
Soon Kyu Lee, Min-Jung Park, Jeong Won Choi, Jin-Ah Baek, Se-Young Kim, Ho Joong Choi, Young Kyoung You, Jeong Won Jang, Pil Soo Sung, Si Hyun Bae, Seung Kew Yoon, Jong Young Choi, Mi-La Cho
Summary: In this study, a patient-derived avatar model was developed to predict immune homeostasis in liver transplantation patients. The model showed promise in evaluating treatment responses and reducing risks to patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Logan C. Ott, Zachary R. Stromberg, Graham A. J. Redweik, Michael J. Wannemuehler, Melha Mellata
Article
Agriculture, Dairy & Animal Science
Angelica Van Goor, Graham A. J. Redweik, Zachary R. Stromberg, Caroline G. Treadwell, Hongwei Xin, Melha Mellata
Article
Microbiology
Graham A. J. Redweik, Mary Kate Horak, Ryley Hoven, Logan Ott, Melha Mellata
Summary: The study found that treating young chickens with probiotics and live vaccines can reduce virulent E. coli plasmids in the intestines, leading to decreased levels of smRNA and potentially reduced siderophore production and antibiotic resistance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jared M. Jochum, Graham A. J. Redweik, Logan C. Ott, Melha Mellata
Summary: The study identified antimicrobial resistant bacteria in commercial poultry farms, including resistance to last-resort antibiotics. These resistant bacteria were found to be potentially transferable among different bacterial strains. The presence of multidrug resistant bacteria in both conventional cage and cage-free poultry indicates the need for further research to develop strategies for mitigation.
Article
Pharmacology & Pharmacy
Zachary R. Stromberg, M. Lisa Phipps, Harsha D. Magurudeniya, Christine A. Pedersen, Trideep Rajale, Chris J. Sheehan, Samantha J. Courtney, Steven B. Bradfute, Peter Hraber, Matthew N. Rush, Jessica Z. Kubicek-Sutherland, Jennifer S. Martinez
Summary: The study presents a tunable, stabilizer-free PLGA nanoparticle formulation capable of encapsulating DNA with exceptional stability and biocompatibility. Various PLGA formulations were tested for stability, with P4 exhibiting the highest stability under physiological conditions. The nanoparticles were found to be as stable as those made with toxic stabilizers like PVA, but significantly less cytotoxic.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Infectious Diseases
Jessica Z. Kubicek-Sutherland, Gary Xie, Migun Shakya, Priya K. Dighe, Lindsey L. Jacobs, Hajnalka Daligault, Karen Davenport, Loreen R. Stromberg, Zachary R. Stromberg, Qiuying Cheng, Prakasha Kempaiah, John Michael Ong'echa, Vincent Otieno, Evans Raballah, Samuel Anyona, Collins Ouma, Patrick S. G. Chain, Douglas J. Perkins, Harshini Mukundan, Benjamin H. McMahon, Norman A. Doggett
Summary: Non-typhoidal Salmonella (NTS) is a major global health concern, particularly in areas affected by malnutrition and comorbidities like HIV and malaria. This study identified a unique NTS isolate named UGA14 that displayed novel plasmid, pseudogene and resistance features, as well as the ability to ferment both lactose and sucrose, highlighting the co-evolution of plasmid-mediated metabolic traits and cephalosporin resistance in NTS strains. The findings emphasize the importance of combined genomic and phenotypic approaches in detecting and characterizing atypical NTS isolates to inform efforts in controlling invasive and antimicrobial resistant NTS.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Review
Chemistry, Analytical
Samantha J. Courtney, Zachary R. Stromberg, Jessica Z. Kubicek-Sutherland
Summary: Influenza virus poses a threat to global health, requiring rapid and accurate diagnostic tests to mitigate the harm of the disease. Nucleic acid-based tests are more accurate than traditional rapid antigen tests, but the high genetic diversity of influenza viruses presents a challenge to the development of robust diagnostic tests.
Article
Biotechnology & Applied Microbiology
Logan C. Ott, Holly J. Appleton, Hu Shi, Kevin Keener, Melha Mellata
Summary: The study demonstrates the efficiency of high voltage atmospheric cold plasma (HVACP) technology for deactivating fungal spores and toxins. Treated spores showed surface ablation and the DON toxin structure was significantly reduced.
Article
Microbiology
Logan C. Ott, Mark Engelken, Sara M. Scott, Elizabeth M. McNeill, Melha Mellata
Summary: This study demonstrates the use of Drosophila as a model for gut-mediated plasmid transfer. Host sex significantly influences the concentrations of donors and recipients in the gut, while transconjugant concentrations are not affected by host sex or genetics and were detected only in specific plasmid groups.
Article
Microbiology
Shelby M. B. Phillips, Carson Bergstrom, Brian Walker, George Wang, Trinidad Alfaro, Zachary R. Stromberg, Becky M. Hess
Summary: This study genetically engineered a lung cell line to create fluorescent reporters for signaling pathways and evaluated the effects of pathogenic and non-pathogenic bacteria on these pathways. The results showed that pathogenic bacteria induced more significant cell death and disruption of signaling compared to non-pathogenic bacteria. This study demonstrates the potential of using ERK-Fra1 signaling as a rapid and sensitive screening method for pathogenic bacteria.
Article
Biology
Graham A. J. Redweik, Michael H. Kogut, Ryan J. Arsenault, Mark Lyte, Melha Mellata
Summary: The study demonstrates that reserpine enhances Salmonella resistance in the chicken intestine by modulating neuro-immunometabolic signaling, leading to reduced bacterial colonization and improved food safety.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Analytical
Samantha J. Courtney, Zachary R. Stromberg, Adan Gutierrez, Daniel Jacobsen, Loreen R. Stromberg, Kiersten D. Lenz, James Theiler, Brian T. Foley, Jason Gans, Karina Yusim, Jessica Z. Kubicek-Sutherland
Summary: Detection methods that do not require nucleic acid amplification are advantageous for rapid viral diagnostics, particularly for influenza detection. Influenza viruses are prone to genetic mutations, making these detection platforms essential for diagnosis.
Article
Biochemical Research Methods
Trinidad Alfaro, Joshua R. Elmore, Zachary R. Stromberg, Janine R. Hutchison, Becky M. Hess
Summary: The CRISPR/Cas9 system has been proven to be a valuable tool for efficient genome editing. This study assessed the suitability of CRISPR/Cas9 for genome editing in Citrobacter freundii and found that it enables high editing efficiency for gene deletion and gene insertion. These results expand the use of CRISPR/Cas9 genome editing to a wider range of microbial organisms.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Meeting Abstract
Biophysics
Samantha J. Courtney, Zachary R. Stromberg, James Theiler, Brian T. Foley, Jason D. Gans, Karina Yusim, Jessica Z. Kubicek-Sutherland
BIOPHYSICAL JOURNAL
(2021)
