Article
Microbiology
Hasan Tukenmez, Pardeep Singh, Souvik Sarkar, Melike Cakir, Ana H. Oliveira, Cecilia Lindgren, Karolis Vaitkevicius, Mari Bonde, A. Elisabeth Sauer-Eriksson, Fredrik Almqvist, Jorgen Johansson
Summary: Bacteria resistant to antibiotics are a major problem, requiring the development of new antimicrobial drugs. In this study, the effectiveness of a new compound, PS900, in inhibiting virulence factor expression in Listeria monocytogenes was demonstrated. Furthermore, PS900 was found to potentiate the bactericidal effects of cholic acid and osmolytes, making it a promising candidate for future antibacterial drug design.
Article
Immunology
Josephine Moran, Liam Feltham, James Bagnall, Marie Goldrick, Elizabeth Lord, Catherine Nettleton, David G. Spiller, Ian Roberts, Pawel Paszek
Summary: The paper investigates the real-time interaction between the food-borne pathogen L. monocytogenes and host macrophages, revealing heterogeneous outcomes of infection and the impact of bacterial quantity on macrophage phagocytosis ability. It also highlights the role of population-level PrfA-mediated activity in regulating host-pathogen interactions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
David R. Mains, Samuel J. Eallonardo, Nancy E. Freitag
Summary: The study shows that L. monocytogenes prfA* mutants exhibit significantly enhanced resistance to oxidative stress. Furthermore, transposon mutagenesis led to the identification of three novel gene targets required for full oxidative stress resistance only in the context of PrfA activation. Additionally, inactivation of cbpA and lmo0779 in a prfA* background resulted in reduced virulence in infected mice.
INFECTION AND IMMUNITY
(2021)
Article
Food Science & Technology
Carolina Ibarguren, M. Virginia Guitian, Romina M. Lenz, Soria M. Cecilia, M. Carina Audisio
Summary: Listeria monocytogenes is a foodborne pathogen that can survive in harsh food processing environments. The use of bacteriocins as a natural alternative to control this pathogen has been hindered by the emergence of resistant strains. This study compares the properties of susceptible and resistant strains, including growth, antibiotic susceptibility, cell morphology, virulence factor expression, and biofilm formation. The results contribute to the understanding of bacteriocin-resistance development in L. monocytogenes and its implications for the use of these peptides as biopreservatives.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Food Science & Technology
Rene Koppel, Jasmin Schade, Martin Peier
Summary: Listeria in food poses a serious risk to consumer health, and reliable methods are needed for detection. While various PCR systems have been developed for identifying Listeria strains, they may not detect all known strains. This study introduces a novel multiplex real-time PCR method that can simultaneously detect multiple common and known Listeria strains.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Svetoslav Dimitrov Todorov, Wilhelm Heinrich Holzapfel, Luis Augusto Nero
Summary: The Pediococcus acidilactici strains isolated from Brazilian artisanal cheese showed high bacteriocin activity against Listeria monocytogenes and Enterococcus spp. The bacteriocins produced by these strains exhibited bactericidal mode of action against certain strains and displayed safety-related genes, although the presence of possible virulence factors and antibiotic resistance should be further investigated.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Fisheries
Qianyu Ji, Junfei Ma, Shuying Wang, Qing Liu
Summary: In this study, the target antigen VP2309 of V. parahaemolyticus was identified and a live vector vaccine strain based on Listeria monocytogenes was successfully constructed, showing immune protection against V. parahaemolyticus infection in zebrafish.
Article
Microbiology
Inna L. Garmasheva, Ljubov T. Oleschenko
Summary: This study aimed to select bacteriocinogenic strains among Enterococcus strains isolated from Ukrainian traditional dairy products. Molasses and steep corn liquor were used as a low-cost media for screening. The results showed that 34 Enterococcus strains produced metabolites that inhibited the growth of indicator strains, and five strains were found to possess enterocin genes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Danielle Nader Furtado, Bernadette Dora Gombossy de Melo Franco, Svetoslav Dimitrov Todorov
Summary: Differentiated Lactococcus lactis bacteriocin producers' strains using repPCR technique. The stability of bacteriocin was found to be influenced by applied chemicals, pH, and temperature. The addition of bacteriocin completely inhibited the growth of Listeria monocytogenes ScottA, Lactobacillus sakei ATCC 15521, or Enterococcus faecium ATCC 19434 in the study.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Infectious Diseases
Pavel A. Andriyanov, Pavel A. Zhurilov, Elena A. Liskova, Tatyana I. Karpova, Elena V. Sokolova, Yulia K. Yushina, Elena V. Zaiko, Dagmara S. Bataeva, Olga L. Voronina, Ekaterina K. Psareva, Igor S. Tartakovsky, Denis V. Kolbasov, Svetlana A. Ermolaeva
Summary: Susceptibility testing of 117 L. monocytogenes strains to 23 antibiotics revealed a varied response, with most strains showing sensitivity to multiple antibiotics but some strains exhibiting resistance to specific drugs. There was a shift in the proportion of resistant strains over time, with different antibiotics showing unique patterns of resistance evolution.
Article
Food Science & Technology
Xiaoli Huang, Yao He, Changcheng Zhong, Kui Zhao, Nagendra P. Shah, Xueying Tao, Hua Wei
Summary: In this study, Lactiplantibacillus plantarum strains were isolated from Hu sheep feces and L. plantarum ELPL14 was identified as a potential probiotic with the ability to inhibit Listeria monocytogenes growth in pasteurized milk. The presence of eleven plantaricin genes in ELPL14 suggested that the inhibition of L. monocytogenes may be due to bacteriocin production. These findings indicate that L. plantarum ELPL14 from Hu sheep has the potential to be used as a natural dairy additive for inhibiting food-borne pathogens, especially L. monocytogenes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Zexuan Song, Shunshi Ji, Yan Wang, Lijuan Luo, Yiqian Wang, Pan Mao, Lingling Li, Huaying Jiang, Changyun Ye
Summary: This study analyzed the whole-genome sequences of 207 ST9 isolates from different sources, geographical locations, and isolated years. The analysis revealed different features in ST9 isolates, such as the presence of plasmids, prophages insertion, premature stop codon mutations in inlA, and the presence of arsenic resistance genes and environment-resistance genes. These findings can help in developing effective strategies to control Listeria monocytogenes in the food chain.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Thulani Sibanda, Elna M. Buys
Summary: This review explores the molecular basis of the connection between stress tolerance responses and pathogenesis of L. monocytogenes. It is found that stress-induced adaptive responses in food can protect the pathogen in the gastrointestinal tract and switch its gene expression from avirulent to virulent state. The review also discusses the overlapping and interlinked regulatory networks for SigB and PrfA.
Article
Multidisciplinary Sciences
Lauren K. Hudson, Harleen K. Chaggar, Claire N. Schamp, Michelle L. Claxton, Daniel W. Bryan, Tracey L. Peters, Yaxiong Song, Catharine R. Carlin, Henk C. den Bakker, Thomas G. Denes
Summary: In this study, two strains of Listeria bacteria obtained from soil samples were phenotypically and genotypically characterized and compared to a reference strain. Genomic analysis showed high nucleotide identity between these strains and the reference strain, and some phenotypic differences were observed.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Samantha Joy D. Valledor, Clarizza May Dioso, Jorge Enrique Vazquez Bucheli, Yu Jin Park, Dong Ho Suh, Eun Sung Jung, Bobae Kim, Wilhelm Heinrich Holzapfel, Svetoslav Dimitrov Todorov
Summary: Enterococcus faecium ST20Kc and ST41Kc isolated from kimchi produce bacteriocins with strong activity against Listeria monocytogenes and various Enterococcus spp. The antimicrobials are proteinaceous and stable, with a bactericidal effect on targeted bacterial strains. Both strains are susceptible to antibiotics and potentially produce additional antimicrobial compounds with metabolomics analysis.