Review
Microbiology
Hicham Bessaiah, Carole Anamale, Jacqueline Sung, Charles M. Dozois
Summary: Pathogens are exposed to harmful conditions in the host environment, and bacterial responses play a crucial role in colonization and pathogenesis. Type 1 fimbriae in E. coli are important colonization factors and their regulation is influenced by signaling and stress. The regulation of type 1 fimbriae is potentially linked to environmental stress responses, and plays a role in regulating colonization and virulence of extraintestinal pathogenic E. coli.
Review
Immunology
Alaullah Sheikh, James M. Fleckenstein
Summary: Pathogenic Escherichia coli can be classified into specific variants (pathovars) based on their behavior and the expression of specific virulence factors. These pathogens interact with CEACAMs through both core elements common to all E. coli and pathovar-specific virulence traits that target the IgV regions of CEACAMs. Recent data suggests that these interactions may not only benefit the pathogen but also provide a mechanism for pathogen elimination.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Bo Hou, Chen-Yan Wang, Shao-Wen Li, Lun-Jiang Zhou, Yong-Liang Che, Qiu-Yong Chen
Summary: The HicAB type II T-A system is abundant in various bacteria and archaea and plays important roles in stress response, virulence, and bacterial persistence. This study demonstrated that the deletion of hicAB significantly decreased the biofilm-forming ability of ExPEC, but the mutant still retained the ability to form curli fimbriae. Further analysis revealed differential gene expression associated with biofilm formation between the hicAB mutant and the parent strain, suggesting that the HicAB system is involved in ExPEC biofilm formation through regulation of outer membrane protein gene expression.
CURRENT MICROBIOLOGY
(2023)
Review
Microbiology
Valerio M. Sora, Gabriele Meroni, Piera A. Martino, Alessio Soggiu, Luigi Bonizzi, Alfonso Zecconi
Summary: The One Health approach highlights the importance of antimicrobial resistance in both public health and food animal production, with specific focus on the ExPEC strains of E. coli which may transfer resistance genes to other pathogenic bacteria, posing a serious threat to public health globally.
Article
Infectious Diseases
Isabella F. S. Figueiredo, Lorena G. Araujo, Raissa G. Assuncao, Itaynara L. Dutra, Johnny R. Nascimento, Fabricia S. Rego, Carolina S. Rolim, Leylane S. R. Alves, Mariana A. Frazao, Samilly F. Cadete, Luis Claudio N. da Silva, Joicy C. de Sa, Eduardo M. de Sousa, Waldir P. Elias, Flavia R. F. Nascimento, Afonso G. Abreu
Summary: The study showed that cinnamaldehyde has potential in treating sepsis by reducing inflammation, increasing cell production in bone marrow and spleen, and improving survival rates in animals.
Article
Immunology
Jin Hu, Dongfang Wang, Xingfa Huang, Yang Yang, Xin Lian, Wenjun Wang, Xiao Xu, Yulan Liu
Summary: This study explores the role of TolC in the pathogenesis of ExPEC strain PPECC42 and finds that TolC is closely associated with the virulence of ExPEC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Catia Longhi, Linda Maurizi, Antonietta Lucia Conte, Massimiliano Marazzato, Antonella Comanducci, Mauro Nicoletti, Carlo Zagaglia
Summary: Multiple-antibiotic-resistant extra-intestinal pathogenic Escherichia coli (ExPEC) is a common cause of nosocomial and community-acquired infections. The coexistence of antibiotic and heavy metal resistance in ExPEC strains is increasing, and the mechanisms for heavy metal tolerance are not fully understood.
Review
Microbiology
Adrianna Aleksandrowicz, Muhammad Moman Khan, Katarzyna Sidorczuk, Mateusz Noszka, Rafal Kolenda
Summary: APEC, a common pathogen in poultry, relies on various adhesins to evade host defense mechanisms and cause infection. Understanding these adhesins is essential for developing effective strategies to combat APEC infections.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Andre Becker S. Saidenberg, Arnoud H. M. van Vliet, Marc Stegger, Thor Bech Johannesen, Torsten Semmler, Marcos Cunha, Alessandro C. de O. Silveira, Eleine Kuroki Anzai, Isabel C. A. Scaletsky, Anders Dalsgaard, Roberto M. La Ragione, Terezinha Knobl
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen associated with human and companion animal diseases, while avian pathogenic pathotype (APEC) severely impacts poultry health and production. The similarities between APEC and human ExPEC suggest a potential for zoonotic transmission.
VETERINARY MICROBIOLOGY
(2022)
Article
Immunology
Ying Yu, Bo Hu, Huanhuan Fan, Hailing Zhang, Shizhen Lian, Hongye Li, Shuangshuang Li, Xijun Yan, Shaohui Wang, Xue Bai
Summary: The study identified molecular epidemiology and biological characteristics of Escherichia coli associated with hemorrhagic pneumonia (HP) in mink from five Chinese provinces. Analysis included serotyping, antimicrobial susceptibility, detection of virulence genes, phylogenetic grouping, whole-genome sequencing, drug resistant gene, multilocus sequence typing (MLST), and biofilm formation assays.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Imed Salhi, Amal Rabti, Asma Dhehibi, Noureddine Raouafi
Summary: Bacterial diseases in livestock animals cause significant economic losses. F17A protein, a crucial virulence factor in pathogenic Escherichia coli, has been identified as a promising therapeutic target and diagnostic tool. Researchers developed anti-F17A antibodies for the detection of this protein and successfully developed a biosensor for electrochemical and fluorescent detection of purified F17A protein and live F17-positive E. coli bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Mingxu Zhou, Yang Yang, Miaomiao Wu, Fang Ma, Yue Xu, Bihua Deng, Jinqiu Zhang, Guoqiang Zhu, Yu Lu
Summary: LPF1 and LPF2 play a role in the adhesion, invasion, and biofilm formation of MPEC, with LPF2 inducing a mild TLR4-independent proinflammatory response.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Maria Belen Sanz, Denise De Belder, J. M. de Mendieta, Diego Faccone, Tomas Poklepovich, Celeste Lucero, Melina Rapoport, Josefina Campos, Ezequiel Tuduri, Mathew O. O. Saavedra, Claudia van der Ploeg, Ariel Roge, Carbapenemases-ExPEC Grp, Fernando Pasteran, Alejandra Corso, Adriana E. E. Rosato, Sonia A. A. Gomez
Summary: This study investigated the distribution and molecular epidemiology of carbapenemase-producing ExPEC in Argentina. The study found a high clonal diversity with CC10 and CC/ST131 being the most common clones. Other international clones were also identified. Further studies are needed to determine the plasmid families responsible for spreading carbapenemases in this region.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Victoria Ballen, Yaiza Gabasa, Carlos Ratia, Melany Sanchez, Sara Soto
Summary: This study characterized 376 extraintestinal pathogenic E. coli strains collected from hospitals in Catalonia, Spain, between 2016 and 2017. The strains showed high resistance to several antibiotics and varied biofilm formation ability. Various virulence and antimicrobial resistance genes were detected, including the colibactin-encoding genes associated with colorectal cancer. The study also investigated the relationship between colibactin and biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Nicky O'Boyle, Gillian R. Douce, Gillian Farrell, Nicholas J. W. Rattray, Mark A. Schembri, Andrew J. Roe, James P. R. Connolly
Summary: The ability of bacterial pathogens to adapt to host niches is driven by the carriage and regulation of genes. The Escherichia coli TF YhaJ exhibits distinct genome-binding dynamics and transcriptome control in different host niches. YhaJ coordinates the activation of fimbriae and the biosynthesis of amino acids.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Neurosciences
Andres de Leon, Julien Gibon, Philip A. Barker
Summary: NGF and BDNF are important trophic factors for sensory neurons during development, but the signaling pathways leading to their death differ, with BDNF-dependent sensory neuron degeneration potentially involving BAX and ROS.
Article
Microbiology
Pascal Rainard, Maryline Reperant-Ferter, Christophe Gitton, Pierre Germon
Summary: The current vaccines for mastitis caused by Escherichia coli are inefficient due to vaccine-induced antibodies failing to bind to most mastitis-associated E. coli strains, hindering phagocytosis. This calls for revised criteria for vaccine evaluation and new vaccine strategies targeting cell-mediated immunity to improve efficiency.
Review
Agriculture, Dairy & Animal Science
Pascal Rainard, Florence B. Gilbert, Pierre Germon, Gilles Foucras
Summary: Infections of the mammary gland are a common disease in dairy ruminants that have negative effects on animal welfare and milk quality. Despite decades of research, the development of mastitis vaccines has not fully met the demand, mainly due to the lack of complete understanding of the immune mechanisms for effective protection.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Sarah Khazaal, Rim Al Safadi, Dani Osman, Aurelia Hiron, Philippe Gilot
Summary: Polyamines are organic polycations involved in biological processes and stress protection. Streptococcus agalactiae lacks the ability to synthesize polyamines but can import them from the media using the PotABCD transporter. The operon containing PotABCD also includes genes related to folate and peptidoglycan biosynthesis, as well as acid stress resistance. Transporter deletion mutants did not show phenotypic traits, indicating potential redundancy with other unidentified transporters.
Article
Genetics & Heredity
Sebastien O. Leclercq, Maxime Branger, David G. E. Smith, Pierre Germon
Summary: The study aimed to analyze the prevalence of LPS core types in the E. coli species and explore their distribution in different phylogenetic groups, as well as their relationship with virulence gene repertoire. Results showed an uneven distribution of core types among phylogroups, with phylogroup A being the most diverse, while other phylogroups were dominated by specific core types like R3 or R1. Strains carrying the LEE virulence operon were mostly of the R3 type across all phylogroups, and within phylogroup B2, strains with a K-12 core belonged to the common STc131 clone of ExPEC strains.
MICROBIAL GENOMICS
(2021)
Review
Immunology
Pascal Rainard, Florence B. Gilbert, Rodrigo P. Martins, Pierre Germon, Gilles Foucras
Summary: Mastitis is a major problem in dairy farming, and the current vaccines are not effective. To address this issue, research should focus on the immunobiology of the mammary gland and the pathogens adapted to it. Vaccines need to elicit immune mechanisms different from those induced by infection, in order to be more effective.
Article
Pharmacology & Pharmacy
Thomas Secher, Elsa Bodier-Montagutelli, Christelle Parent, Laura Bouvart, Melanie Cortes, Marion Ferreira, Ronan MacLoughlin, Guy Ilango, Otmar Schmid, Renaud Respaud, Nathalie Heuze-Vourc'h
Summary: The study investigated the impact of aggregates produced during the aerosolization of therapeutic antibodies on the immune system. It was found that aggregated antibodies led to immune cell activation and subsequent cell death.
Article
Neurosciences
Andres de Leon, Julien Gibon, Philip A. Barker
Summary: This study reveals the pro-degenerative role of APP in the development of the sensory nervous system during development and highlights its importance in the regulation of calcium signaling in sensory neurons.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Microbiology
Sarah Khazaal, Rim Al Safadi, Dani Osman, Aurelia Hiron, Philippe Gilot
Summary: This study identified a transporter in Streptococcus agalactiae that plays a role in survival during citric acid stress at pH 4.0 in the presence of spermidine. The findings suggest a new understanding of the molecular mechanisms enabling S. agalactiae to survive at the physiological pH of the vagina.
Article
Biochemistry & Molecular Biology
E. Ivanova, C. Hue-Beauvais, A. Chaulot-Talmon, J. Castille, J. Laubier, C. De Casanove, A. Aubert-Frambourg, P. Germon, H. Jammes, F. Le Provost
Summary: Mastitis has negative effects on both human breastfeeding and animal farming, but the exact impact on mammary tissue is not fully understood. This study investigates the changes in DNA methylation caused by inflammation and lactation rank in mouse mammary tissue. The findings suggest that lactation rank has a stronger influence on DNA methylation than the onset of inflammation. This research provides valuable insights into the epigenetic regulation of lactation in different conditions.
Article
Biochemistry & Molecular Biology
E. Ivanova, C. Hue-Beauvais, A. Chaulot-Talmon, J. Castille, J. Laubier, C. De Casanove, A. Aubert-Frambourg, P. Germon, H. Jammes, F. Le Provost
Summary: Mastitis is a common reason for ceasing breastfeeding and causing economic losses in farm animals. This study investigated the effect of inflammation on mouse mammary tissue, specifically the changes in DNA methylation. The analysis focused on comparing inflammation during lactation periods and the relationship with gene expression and DNA methylation changes. The findings suggest that inflammation and lactation rank, as well as previous inflammation history, play important roles in determining epigenetic regulation.
Article
Agriculture, Dairy & Animal Science
Marion Rambault, Florence B. Gilbert, Philippe Roussel, Alexia Tessier, Pierre Germon, Nathalie Winter, Aude Remot
Summary: Bovine mastitis, caused by bacterial infection, not only leads to economic losses and health problems in animals, but also affects their welfare. Neutrophils play a crucial role in eliminating pathogens and are responsible for the increase in somatic cell count during mastitis. Recent research reveals that a subset of neutrophils expressing major histocompatibility complex class II have regulatory capacities in cattle, providing new insights into the immune response during mastitis.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
L. Ginger, D. Ledoux, M. Bouchon, I. Rautenbach, C. Bagnard, T. Lurier, G. Foucras, P. Germon, D. Durand, A. de Boyer des Roches
Summary: This study aimed to determine the effect of LPS-induced mastitis with or without NSAID on dairy cows' responses and to evaluate the specificity and sensitivity of behavioral responses in detecting cows with mastitis. The results suggest that feeding/ruminating, tail position, and reactivity at forestripping could be used as behavioral indicators for early detection of mastitis-related pain in dairy cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Review
Agriculture, Dairy & Animal Science
Pierre Germon, Rodrigo Prado Martins
Summary: The mammary gland in ruminants plays a crucial role in providing immune protection and nutrition to suckling youngs. This review explores the innate and inducible immune mechanisms of the mammary gland and discusses the remaining knowledge gaps needed for strategies to enhance mammary immune responses.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
Pablo Gomes Noleto, Florence B. B. Gilbert, Christelle Rossignol, Patricia Cunha, Pierre Germon, Pascal Rainard, Rodrigo Prado Martins
Summary: In this study, an ex vivo model using punch-excised teat tissue was used to investigate the immune mechanisms of the mammary gland in the early stages of infection. The results showed that the teat tissue responded to TLR agonists and bacteria, simulating the inflammatory response caused by infection. The study also demonstrated that this ex vivo model can be applied to frozen-stored tissue explants.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)