Article
Microbiology
Yanli Du, Xiangyu Wang, Zongli Han, Ying Hua, Kaina Yan, Bao Zhang, Wei Zhao, Chengsong Wan
Summary: The study demonstrates the essential role of PPK1 in both survival and virulence of EHEC O157:H7, with deletion of ppk1 gene leading to decreased survival rate under heat stress and acid tolerance, as well as reduced adhesion, formation of attaching and effacing lesions, invasion ability, and overall virulence in vivo. These findings suggest that PPK1 is crucial for the pathogenesis of EHEC O157:H7.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Stefan Toth, Zuzana Fagova, Monika Holodova, Dema Zeidan, Patrick Hartel, Kristina Curgali, Eva Mechirova, Milan Maretta, Radomira Nemcova, Sona Gancarcikova, Marianna Dankova
Summary: This study aimed to investigate the influence of Escherichia coli (E. coli) on the intestinal barrier. The results showed that the colon of germ-free piglets was more resistant to coliform bacterial infection compared to the jejunum. Additionally, the intestinal barrier exhibited a rapid response and better preparation for possible pathological attacks.
Article
Microbiology
Alejandro Huerta-Saquero, Itziar Chapartegui-Gonzalez, Sarah Bowser, Nittaya Khakhum, Jacob L. Stockton, Alfredo G. Torres
Summary: This study demonstrates the potential of using P22 viral-like particles as nanocontainers for delivering immunogenic proteins of enterohemorrhagic Escherichia coli (EHEC) and creating effective vaccines against EHEC infections. The EscC-P22 particles were able to induce specific antibodies in mice, enhance phagocytic activity against EHEC, and reduce bacterial adherence to epithelial cells, indicating their functionality. These findings suggest that EscC-P22-based particles could be a promising nanovaccine candidate for immunization against EHEC O157:H7 infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Lang Peng, Rexford Mawunyo Dumevi, Marco Chitto, Nadja Haarmann, Petya Berger, Gerald Koudelka, Herbert Schmidt, Alexander Mellmann, Ulrich Dobrindt, Michael Berger
Summary: This study proposes a new method for genetic manipulation of enterohemorrhagic Escherichia coli (EHEC) that is more efficient than traditional methods. The method increases recombineering efficiency by 3-4 times in EHEC O157:H7 EDL933 Delta stx1/2, but shows similar efficiency in Escherichia coli K-12 MG1655. Additionally, the study suggests that the inhibition of the RecFOR pathway indirectly blocks phage induction and improves recombineering efficiency.
Review
Gastroenterology & Hepatology
Lingyan Jiang, Wen Yang, Xinlei Jiang, Ting Yao, Lu Wang, Bin Yang
Summary: EHEC O157 carries 177 unique genomic islands, termed O islands, which play a crucial role in its pathogenicity by providing virulence factors, effectors, and regulatory proteins.
Review
Food Science & Technology
Xiaoyan Zhao, Yeting Sun, Yue Ma, Yujia Xu, Hongyang Guan, Dan Wang
Summary: Enterohemorrhagic Escherichia coli (EHEC) is a major bacterial pathogen that can survive in food such as meat and vegetables. Understanding the impact of vegetable characteristics on the adhesion and proliferation processes of EHEC is important for developing control measures. This review discusses the amount, methods, and pathways of adhesion, as well as the proliferation and biofilm formation processes of EHEC during vegetable contamination. Factors such as vegetable types, cultivars, tissue characteristics, and damage levels can affect EHEC adhesion on vegetables.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Joshua L. Cherry
Summary: Enterohemorrhagic E. coli (EHEC) is a major cause of human illness, death, and economic loss. Understanding the genetic changes in EHEC and its source of contamination is crucial for effective mitigation. Through whole-genome sequencing, it was found that EHEC experienced genetic changes mainly in adhesion-related sequences, potentially leading to altered adhesive properties and increased virulence. This pattern of genetic change may help identify other bacteria threatening human health. Additionally, the presence of a mutation affecting arsenic resistance regulation suggests an arsenic-contaminated groundwater as the persistent source of contamination. This observation can aid in locating the reservoir of EHEC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
So Young Yang, Ki Sun Yoon
Summary: Listeria monocytogenes and Entero-hemorrhagic Escherichia coli (EHEC) can survive in refrigerated yogurt. The risk of L. monocytogenes and EHEC due to yogurt consumption was estimated using predictive survival models. The results show that the foodborne illness risk from L. monocytogenes and EHEC due to yogurt consumption is very low, but controlling the initial contamination level of EHEC during yogurt manufacture should be emphasized.
Article
Biochemistry & Molecular Biology
Jun-Young Park, Cheorl-Ho Kim, Seung-Hak Cho
Summary: In this study, we developed a FimH inhibitor to prevent adhesion of enterohemorrhagic Escherichia coli on human intestinal epithelium during the early stage of infection. By investigating the ligand binding domain of FimH and analyzing its evolutionary characteristics, we identified a potential drug target and found that glycan-like peptides can serve as initial infection inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Yansong Xue, Mei-Jun Zhu
Summary: This article discusses how enterohemorrhagic Escherichia coli (EHEC), specifically E. coli O157:H7, hijacks the host immune system and interferes with host signaling pathways. It also highlights the promising role of bioactive compounds derived from food and probiotics in harnessing host innate immunity and eliminating E. coli O157:H7 infection with multiple strategies.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Microbiology
Dawei Yang, Yongwu Yang, Pengfei Qiao, Fengwei Jiang, Xinyang Zhang, Zihui Zhao, Tao Cai, Ganwu Li, Wentong Cai
Summary: This study identified a two-component signaling system in EHEC that promotes adaptability and virulence expression in response to mannose, a sugar found in the intestinal mucus.
Article
Food Science & Technology
Kosuke Takada, Kotone Hama, Takaomi Sasaki, Yuichi Otsuka
Summary: The TA genetic modules play important roles in controlling bacterial events, while hokW-sokW in Sp5 phage serves as a novel function in facilitating the release of phage progeny through E. coli lysis.
Article
Gastroenterology & Hepatology
Stefanie L. Vogt, Antonio Serapio-Palacios, Sarah E. Woodward, Andrew S. Santos, Stefan P. W. de Vries, Michelle C. Daigneault, Lisa V. Brandmeier, Andrew J. Grant, Duncan J. Maskell, Emma Allen-Vercoe, B. Brett Finlay
Summary: Enterohemorrhagic Escherichia coli (EHEC) adjusts its metabolism and activates stress responses to adapt to the presence of gut microbiota metabolites. Understanding the molecular mechanisms by which pathogens adapt to competing microbes in the host environment may lead to the development of therapies to enhance colonization resistance and prevent infection.
Article
Chemistry, Multidisciplinary
Jordan P. Hempfling, Emily R. Sekera, Amar Sarkar, Amanda B. Hummon, Dehua Pei
Summary: Efficient and convenient method for producing recombinant proteins with free N-terminal cysteines has been reported. Site-specific labeling of the resulting proteins at the N-terminus was achieved through a novel genetic fusion and sequential enzymatic processing.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Microbiology
Matthew K. Schnizlein, Kimberly C. Vendrov, Summer J. Edwards, Eric C. Martens, Vincent B. Young
Article
Immunology
Katie Saund, Krishna Rao, Vincent B. Young, Evan S. Snitkin
OPEN FORUM INFECTIOUS DISEASES
(2020)
Editorial Material
Medical Laboratory Technology
Eric M. Ransom, Carey-Ann D. Burnham, Lee Jones, Colleen S. Kraft, L. Clifford McDonald, Andrew R. Reinink, Vincent B. Young
CLINICAL CHEMISTRY
(2020)
Article
Immunology
Lisa Abernathy-Close, Michael G. Dieterle, Kimberly C. Vendrov, Ingrid L. Bergin, Krishna Rao, Vincent B. Young
INFECTION AND IMMUNITY
(2020)
Article
Microbiology
Michael G. Dieterle, Rosemary Putter, D. Alexander Perry, Anitha Menon, Lisa Abernathy-Close, Naomi S. Perlman, Aline Penkevich, Alex Standke, Micah Keidan, Kimberly C. Vendrov, Ingrid L. Bergin, Vincent B. Young, Krishna Rao
Article
Microbiology
Lisa Abernathy-Close, Madeline R. Barron, James M. George, Michael G. Dieterle, Kimberly C. Vendrov, Ingrid L. Bergin, Vincent B. Young
Summary: Clostridioides difficile (C. difficile) infection poses significant risks to patients with inflammatory bowel disease (IBD), leading to increased morbidity and mortality. The development of IBD alters the gut microbiome and decreases colonization resistance to C. difficile, making animals susceptible to C. difficile colonization and infection. Inflammation induced by IBD plays a critical role in overcoming colonization resistance and promoting C. difficile colonization in the host.
Review
Gastroenterology & Hepatology
Matthew K. Schnizlein, Vincent B. Young
Summary: Clostridioides difficile infection is a significant burden on health and economy worldwide. This review provides insights into the physiology of C. difficile, its survival mechanisms in the gut environment, and the relationships between host-microorganism and microorganism-microorganism.
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
(2022)
Review
Microbiology
Madeline R. Barron, Vincent B. Young
Summary: The relationship between the gut environment and bacterial colonization is of great significance. The complex interactions between multiple factors in the gut environment make it difficult to uncover the characteristics of the system using reductionist methods. The systems biology approach provides a more comprehensive perspective. This article explores the relationship between the gut environment and bacterial colonization from the perspective of systems biology, and provides guidance for future research.
Article
Microbiology
Madeline R. Barron, Kelly L. Sovacool, Lisa Abernathy-Close, Kimberly C. Vendrov, Alexandra K. Standke, Ingrid L. Bergin, Patrick D. Schloss, Vincent B. Young
Summary: Patients with inflammatory bowel disease (IBD) have an increased risk of developing C. difficile infection (CDI), even in the absence of antibiotic treatment. The mechanisms regulating C. difficile colonization in IBD patients remain unclear. In this study, a mouse model was used to demonstrate that intestinal inflammation alters microbiota composition, leading to susceptibility to C. difficile colonization. Treating inflammation with an anti-p40 monoclonal antibody restores colonization resistance. Microbiota transfer experiments confirm that the microbiota shaped in the setting of IBD is the primary driver of susceptibility to C. difficile colonization.
Article
Microbiology
Nicholas A. Lesniak, Alyxandria M. Schubert, Kaitlin J. Flynn, Jhansi L. Leslie, Hamide Sinani, Ingrid L. Bergin, Vincent B. Young, Patrick D. Schloss
Summary: Clostridioides difficile colonization can lead to a range of infection severities, from mild diarrhea to life-threatening conditions. While factors such as patient age, blood cell count, and C. difficile characteristics have been associated with disease severity, the role of specific gut bacteria in influencing severity is unclear. This study demonstrates that different gut bacterial communities can contribute to variations in Clostridioides difficile infection outcomes. Certain bacterial groups were associated with more severe outcomes, while others were associated with milder outcomes. Incorporating gut bacterial community data into severity models may aid in identifying at-risk patients and improving interventions.
Article
Public, Environmental & Occupational Health
Meghana Kamineni, Erkin Otles Meng, Jeeheh Oh, Krishna Rao, Vincent B. Young, Benjamin Y. Li, Lauren R. West, David C. Hooper, Erica S. Shenoy, John G. Guttag, Jenna Wiens, Maggie Makar
Summary: This article presents the prospective and retrospective evaluations of two CDI infection risk prediction models and discusses their robustness to data-set shifts.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Patricia P. Bloom, Krishna Rao, Christine Bassis, Borko Nojkov, Vincent B. Young, Anna S. F. Lok
Summary: Patients with cirrhosis have higher duodenal epithelial permeability and lower mucosal bacteria alpha diversity compared to controls, while no such differences are seen in the ileum or colon. Specific bacteria are associated with epithelial permeability and future hepatic decompensation.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Veronica Azcutia, Matthias Kelm, Seonyoung Kim, Anny-Claude Luissint, Sven Flemming, Lisa Abernathy-Close, Vincent B. Young, Asma Nusrat, Mark J. Miller, Charles A. Parkos
Summary: This study used real-time intravital microscopy and a new distal colon loop model to investigate PMN migration dynamics in response to acute colonic injury models. The results revealed different migratory patterns of PMNs under various conditions, suggesting potential mechanisms for regulating PMN migration and providing insights for future anti-inflammatory and pro-repair therapies.
Article
Microbiology
Madeline R. Barron, Roberto J. Cieza, David R. Hill, Sha Huang, Veda K. Yadagiri, Jason R. Spence, Vincent B. Young
Meeting Abstract
Gastroenterology & Hepatology
Hiroko Kitamoto, Peter Higgins, Vincent Young, Nobuhiko Kamada