Article
Multidisciplinary Sciences
Carl-Philipp Hackstein, Dana Costigan, Linnea Drexhage, Claire Pearson, Samuel Bullers, Nicholas Ilott, Hossain Delowar Akther, Yisu Gu, Michael E. B. FitzPatrick, Oliver J. Harrison, Lucy C. Garner, Elizabeth H. Mann, Sumeet Pandey, Matthias Friedrich, Nicholas M. Provine, Holm H. Uhlig, Emanuele Marchi, Fiona Powrie, Paul Klenerman, Emily E. Thornton
Summary: Interactions between commensal microbes and the host have a significant impact on the host's immune system and are crucial in human health and disease. Certain T cells in the colon exhibit innate-like characteristics and are reactive to commensal microbes. These cells are increased in ulcerative colitis patients and may contribute to the pathogenesis of colitis.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Judith Wienke, Saskia R. Veldkamp, Eva M. Struijf, Fjodor A. Yousef A. Yengej, M. Marlot van der Wal, Annet van Royen-Kerkhof, Femke van Wijk
Summary: This study found that activated endothelial cells can induce sustained CD69 expression, increased cytokine response, and a phenotypic profile reminiscent of tissue-resident memory T cells (TRM) in T cells. The interaction with activated endothelial cells during transmigration plays an important role in the differentiation and residency of TRM cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Cherng-Shyang Chang, Yi-Chu Liao, Chih-Ting Huang, Chiao-Mei Lin, Chantal Hoi Yin Cheung, Jhen-Wei Ruan, Wen-Hsuan Yu, Yi-Ting Tsai, I-Jung Lin, Chien-Hsun Huang, Jong-Shian Liou, Ya-Hsien Chou, Hung-Jen Chien, Hsiao-Li Chuang, Hsueh-Fen Juan, Hsuan-Cheng Huang, Hong-Lin Chan, Yu-Chieh Liao, Shiue-Cheng Tang, Yu-Wen Su, Tse-Hua Tan, Andreas J. Baeumler, Cheng-Yuan Kao
Summary: The knockout of Dusp6 enhances baseline colon barrier integrity and ameliorates DSS-induced colonic injury, accompanied by altered glucose metabolism and increased mitochondrial oxygen consumption in Caco-2 cells. Dusp6-knockout mice are more resistant to DSS-induced dysbiosis, and a member of the genus Duncaniella in the gut microbiota confers host protection from injury.
Article
Multidisciplinary Sciences
Xiangjun Liu, Shanzhao Jin, Simeng Hu, Ruoyan Li, Haihao Pan, Yi Liu, Pan Lai, Deshu Xu, Jingru Sun, Ziyang Liu, Yumei Gao, Yifan Zhao, Fengjie Liu, Yu Xiao, Yingyi Li, Yujie Wen, Zhuojing Chen, Bufang Xu, Yuchieh Lin, Menglong Ran, Qianxi Li, Shuxia Yang, Hang Li, Ping Tu, Muzlifah Haniffa, Sarah A. Teichmann, Fan Bai, Yang Wang
Summary: Cutaneous T cell lymphoma (CTCL) is a heterogeneous group of non-Hodgkin lymphoma characterized by clonal malignant T cells. This study analyzed CTCL patient samples using single-cell RNA-seq, TCR and whole-exome sequencing, revealing the molecular profiles of malignant T cells and their association with the microenvironment and clinical outcomes.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Seon Ah Lim, Jun Wei, Thanh-Long M. Nguyen, Hao Shi, Wei Su, Gustavo Palacios, Yogesh Dhungana, Nicole M. Chapman, Lingyun Long, Jordy Saravia, Peter Vogel, Hongbo Chi
Summary: Regulatory T cells play a crucial role in immune tolerance and immunosuppression in the tumor microenvironment. Inhibiting lipid synthesis dependent on SREBPs in T-reg cells can enhance antitumor immune responses without autoimmune toxicity, and deletion of SCAP in these cells inhibits tumor growth and enhances immunotherapy. SCAP and SREBPs signaling regulate lipid synthesis and inhibitory receptor signaling in T-reg cells, pointing to new ways of targeting these cells for cancer therapy.
Article
Microbiology
Liaoxun Lu, Tianhan Li, Xinyu Feng, Zhilong Liu, Yang Liu, Tianzhu Chao, Yanrong Gu, Rong Huang, Fanghui Zhang, Le He, Binhui Zhou, Eryan Kong, Zhuangzhuang Liu, Xugang Wang, Zhijun Chen, Hui Wang, Marie Malissen, Bernard Malissen, Lichen Zhang, Yinming Liang
Summary: Schistosomiasis, a parasitic infection affecting over 200 million people worldwide, can induce the expression of PD-1 in T cells of the liver. Loss of PD-1 leads to an increase in T cell count and a shift in cytokine production in infected mice, but does not result in the eradication of parasites or reduction in pathogen burden.
Article
Multidisciplinary Sciences
Rosalind L. Ang, Mark Chan, Diana Legarda, John P. Sundberg, Shao-Cong Sun, Virginia L. Gillespie, Nicholas Chun, Peter S. Heeger, Huabao Xiong, Sergio A. Lira, Adrian T. Ting
Summary: The study found that the phenotype of Sharpin(cpdm/cpdm) mice is dependent on CYLD, which suppresses TNF- and RIPK1-dependent cell death. Regulation of CYLD can ameliorate inflammatory symptoms such as dermatitis in mice.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cardiac & Cardiovascular Systems
Jessica Handke, Laura Kummer, Markus A. Weigand, Jan Larmann
Summary: The study found that preoperative expansion of Tregs can mitigate the destabilization of atherosclerotic plaques induced by surgical stress. This suggests that therapeutic expansion of Tregs prior to major surgery may help prevent cardiovascular events associated with non-cardiac surgery.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Immunology
Inge C. M. Hoogland, Jutka Yik, Dunja Westhoff, Joo-Yeon Engelen-Lee, Merche Valls Seron, Wing Kit Man, Judith H. P. M. Houben-Weerts, Michael W. T. Tanck, David J. van Westerloo, Tom van der Poll, Willem A. van Gool, Diederik van de Beek
Summary: This study found that functional loss of alpha 7nAChR during systemic infection led to increased expression of INF-alpha and IL-6 in the brain, but did not result in distinct differences in microglial cell number or morphological activation of microglia.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Immunology
Chia-Hao Lin, Cheng-Jang Wu, Sunglim Cho, Rasika Patkar, William J. Huth, Ling-Li Lin, Mei-Chi Chen, Elisabeth Israelsson, Joanne Betts, Magdalena Niedzielska, Shefali A. Patel, Han G. Duong, Romana R. Gerner, Chia-Yun Hsu, Matthew Catley, Rose A. Maciewicz, Hiutung Chu, Manuela Raffatellu, John T. Chang, Li-Fan Lu
Summary: This study reveals that intestinal T-reg cells produce IL-27 to regulate T(H)17 cell immunity, leading to exacerbated intestinal inflammation and colitis-associated cancer, but also providing protection against enteric bacterial infection. A CD83(+)CD62L(lo) T-reg cell subset is identified as the main IL-27 producer.
Article
Oncology
Thi-Ngoc Nguyen, Hieu-Huy Nguyen-Tran, Chen-Yun Chen, Tien Hsu
Summary: This study identifies the molecular and cellular mechanisms underlying the interaction between VHL-deficient kidney tubule cells and macrophages in the tumor microenvironment, providing potential targets for the prevention and treatment of ccRCC.
Article
Endocrinology & Metabolism
Ana Teijeiro, Amanda Garrido, Anna Ferre, Cristian Perna, Nabil Djouder
Summary: The study demonstrates that inhibiting the IL-17A axis can suppress diet-induced obesity and metabolic disorders, while promoting adipose tissue browning, thermogenesis, and energy expenditure. IL-17A also induces pathological changes in adipocytes, leading to obesity and metabolic syndrome.
Article
Biochemistry & Molecular Biology
Ibukun Elizabeth Osadare, Ling Xiong, Ignacio Rubio, Ute Neugebauer, Adrian T. Press, Anuradha Ramoji, Juergen Popp
Summary: Sepsis is a life-threatening condition caused by an excessive and disproportionate immune response to an infection. This study used Raman spectroscopy to analyze the changes in splenic T-lymphocyte profiles during bacterial sepsis and hyperinflammatory endotoxemia. The results showed significant changes in DNA region of splenocytes during sepsis, indicating notable transcriptomic activity. Additionally, splenocytes exposed to high-dose lipopolysaccharide injection displayed significant protein and lipid profile changes, with variations in inflammation severity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Parameswaran Gangadharan Sreekumar, Feng Su, Christine Spee, Eduardo Araujo, Steven Nusinowitz, Srinivasa T. Reddy, Ram Kannan
Summary: Lipid peroxidation caused by oxidative stress is a major factor in age-related macular degeneration (AMD). This study aims to investigate the role of LDLR in the NaIO3-induced model of dry AMD. The results indicate that LDLR loss leads to lipid accumulation and impaired retinal function, which may contribute to the development of AMD.
Article
Immunology
Ruth Pia Duecker, Lucia Gronau, Patrick C. Baer, Stefan Zielen, Ralf Schubert
Summary: Our study focused on investigating the feasibility of different approaches of hematopoietic stem cell transplantation (HSCT) for Ataxia-telangiectasia (A-T) by using Atm-deficient mice as models. The results showed that haploidentical HSCT could extend the lifespan of Atm-deficient mice and improve T-cell numbers and functionality. Interestingly, HSCT using heterozygous donor cells also led to improved survival and enhanced CD4 cell functionality in Atm-deficient mice, suggesting it as a potential strategy for A-T treatment.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Immunology
Stefan H. E. Kaufmann
Article
Immunology
Mark J. Geels, Regitze L. Thogersen, Carlos A. Guzman, Mei Mei Ho, Frank Verreck, Nicolas Collin, James S. Robertson, Samuel J. McConkey, Stefan H. E. Kaufmann, Odile Leroy
Article
Immunology
J. Maertzdorf, S. H. E. Kaufmann, J. Weiner
Article
Immunology
January Weiner, Stefan H. E. Kaufmann, Jeroen Maertzdorf
Article
Microbiology
Macarena Beigier-Bompadre, Georgina N. Montagna, Anja A. Kuehl, Laura Lozza, January Weiner, Andreas Kupz, Alexis Vogelzang, Hans-Joachim Mollenkopf, Delia Loewe, Silke Bandermann, Anca Dorhoi, Volker Brinkmann, Kai Matuschewski, Stefan H. E. Kaufmann
Article
Multidisciplinary Sciences
Fadhil Ahsan, Jeroen Maertzdorf, Ute Guhlich-Bornhof, Stefan H. E. Kaufmann, Pedro Moura-Alves
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Andreas Kupz, Ulrike Zedler, Manuela Staeber, Stefan H. E. Kaufmann
Article
Biology
Paul F. McKay, Deniz Cizmeci, Yoann Aldon, Jeroen Maertzdorf, January Weiner, Stefan H. E. Kaufmann, David J. M. Lewis, Robert A. van den Berg, Giuseppe Del Giudice, Robin J. Shattock
Article
Cell Biology
Gopinath Krishnamoorthy, Peggy Kaiser, Ulrike Abu Abed, January Weiner, Pedro Moura-Alves, Volker Brinkmann, Stefan H. E. Kaufmann
DISEASE MODELS & MECHANISMS
(2020)
Review
Medicine, Research & Experimental
Anca Dorhoi, Leigh A. Kotze, Jay A. Berzofsky, Yongjun Sui, Dmitry I. Gabrilovich, Ankita Garg, Richard Hafner, Shabaana A. Khader, Ulrich E. Schaible, Stefan H. E. Kaufmann, Gerhard Walz, Manfred B. Lutz, Robert N. Mahon, Suzanne Ostrand-Rosenberg, William Bishai, Nelita du Plessis
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Immunology
John S. Tregoning, January Weiner, Deniz Cizmeci, Danielle Hake, Jeroen Maertzdorf, Stefan H. E. Kaufmann, Geert Leroux-Roels, Cathy Maes, Annelies Aerssens, Anna Calvert, Christine E. Jones
Article
Immunology
Natalie E. Nieuwenhuizen, Joanna Zyla, Ulrike Zedler, Silke Bandermann, Ulrike Abu Abed, Volker Brinkmann, Stefan H. E. Kaufmann
Summary: BCG, the only available vaccine against tuberculosis, was found to have varying efficacy against pulmonary TB based on age and sex. This study revealed that female mice had better protection from BCG vaccination against M. tuberculosis H37Rv challenge, showing lower lung bacterial burdens and less weight loss compared to male mice. It suggests the importance of including both male and female mice in preclinical testing of vaccine candidates.
Article
Biochemistry & Molecular Biology
Elisabeth M. Liebler-Tenorio, Johannes Heyl, Nadine Wedlich, Julia Figl, Heike Koehler, Gopinath Krishnamoorthy, Natalie E. Nieuwenhuizen, Leander Grode, Stefan H. E. Kaufmann, Christian Menge
Summary: This study compares the development of tuberculous granulomas at the site of BCG vaccination and its recombinant derivatives in goats. The results show that granulomas with central caseous necrosis are induced by both BCG and its recombinant derivatives, although the size and extent of necrosis differ between vaccine strains. The recombinant derivatives induce fewer CD4(+) T and B cells and more CD8(+) cells in the granulomas compared to the parental BCG strain. Furthermore, the numbers of multinucleated giant cells (MNGCs) and cells with lipid bodies are significantly lower in groups administered with the recombinant BCG strains. These findings suggest that the granulomas induced by BCG and its recombinant derivatives in goats can serve as suitable models to compare reactions to different mycobacteria or tuberculosis vaccines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Immunology
Aldo Tagliabue, Diana Boraschi, Luciana C. C. Leite, Stefan H. E. Kaufmann
Article
Biochemistry & Molecular Biology
Julia Figl, Heike Koehler, Nadine Wedlich, Elisabeth M. Liebler-Tenorio, Leander Grode, Gerald Parzmair, Gopinath Krishnamoorthy, Natalie E. Nieuwenhuizen, Stefan H. E. Kaufmann, Christian Menge
Summary: A more effective vaccine against tuberculosis is urgently needed. The recombinant vaccine VPM1002 has been found to be safer and more efficacious than the existing vaccine BCG in mice models. In this study, the safety and immunogenicity of VPM1002 and its derivatives, PDX and NUOG, were assessed in juvenile goats. The results showed that VPM1002 and NUOG induced anti-tuberculous immunity with a comparable safety profile to BCG.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)