Article
Multidisciplinary Sciences
Lauren E. Stopfer, Nicholas J. Rettko, Owen Leddy, Joshua M. Mesfin, Eric Brown, Shannon Winski, Bryan Bryson, James A. Wells, Forest M. White
Summary: Combining multiple therapeutic strategies may improve survival benefits for NRAS/BRAF mutant melanoma patients. This study utilized immunopeptidomics to measure the impact of MEK inhibition on anti-tumor immunity and identified enhanced expression of specific tumor antigens following therapy. Targeting these upregulated tumor antigens can enhance cell killing.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
Laura C. Demmers, Wei Wu, Albert J. R. Heck
Summary: HLA molecules play critical roles in the adaptive immune system by presenting small peptides to signal cell health status to the immune system. This study investigated the adaptive response of a B lymphoblastic cell line to high temperature treatment, revealing potential preparations for immune-like responses in the absence of invading pathogenic peptides. The findings suggest intriguing temperature-sensitive adaptations in this particular B cell line.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Review
Cell Biology
Sofya A. Erokhina, Maria A. Streltsova, Leonid M. Kanevskiy, Maria V. Grechihina, Alexander M. Sapozhnikov, Elena I. Kovalenko
Summary: HLA-DR+ NK cells display characteristics of both NK cells and dendritic cells, and are considered significant players in the innate immune system due to their functional activities in producing cytokines, degranulation, and antigen presentation to T cells.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Editorial Material
Immunology
Antonius A. de Waard, Tamara Verkerk, Marlieke L. M. Jongsma, Kelly Hoefakker, Sunesh Sethumadhavan, Carolin Gerke, Sophie Bliss, Xiangrui Kong, George M. C. Janssen, Arnoud H. de Ru, Frans H. J. Claas, Arend Mulder, Robert Tampe, Peter A. van Veelen, Anne Halenius, Robbert M. Spaapen
Summary: PAKC is a novel panel of ten cell lines that have individual components of the HLA class I antigen presentation pathway knocked out, providing a single model system for studies across multiple areas. This system is expected to accelerate research in oncology, infectiology, and autoimmunity.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Se-Jin Kim, Elham Karamooz
Summary: This review discusses the current understanding of MR1 and HLA-E antigen presentation in the context of Mycobacterium tuberculosis infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Liran Komov, Dganit Melamed Kadosh, Eilon Barnea, Arie Admon
Summary: This study found that interferons can promote the rapid degradation of defective ribosomal products (DRiPs), leading to a change in the proportion of DRiP-derived MHC peptides, which is crucial for combating viral infections.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Oncology
Lei Zhan, Junhui Zhang, Jing Zhang, Xiaojing Liu, Suding Zhu, Yuchuan Shi, Yu He, Wenyan Wang, Yijing Wei, Zhenhai Tang, Guo Chen, Bing Wei, Yunxia Cao
Summary: In this study, the researchers found that upregulated autophagy and decreased expression of MHC-I and NLRC5 were associated with endometrial cancer (EC). Inhibition of autophagy was found to suppress MHC-I gene expression. They also discovered that LC3 interacted with NLRC5 to inhibit the NLRC5-mediated MHC-I antigen presentation pathway. These findings suggest that inhibiting LC3 and promoting NLRC5 may be a promising immunotherapy strategy for EC management.
Article
Immunology
Soumya Mukherjee, Alvaro Sanchez-Bernabeu, Laura C. Demmers, Wei Wu, Albert J. R. Heck
Summary: Mass-spectrometry based immunopeptidomics has revealed a vast ligandome of self and cancer neoantigens, with a focus on a small subset of post-translationally modified HLA Class I peptides, primarily derived from nuclear proteins such as transcription factors. These O-GlcNAcylated HLA Class I peptides are preferentially presented by the HLA-B*07:02 allele, which has a Proline residue anchor and a binding groove that accommodates the consensus sequence for O-GlcNAcylation. These observations may aid in predicting novel O-GlcNAcylated HLA antigens best presented by patients with HLA-B*07:02 or related alleles using Proline as an anchoring residue.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yi Sun, Michael C. Young, Claire H. Woodward, Julia N. Danon, Hau Truong, Sagar Gupta, Trenton J. Winters, Joan Font-Burgada, George M. Burslem, Nikolaos G. Sgourakis
Summary: This study created stable MHC-I molecules capable of accepting low-to moderate-affinity peptides through an engineered disulfide bond bridging conserved epitopes. The effects of the disulfide bond on the conformation of the MHC-I structure were characterized using solution NMR. The structure-guided design provides a universal platform for screening antigenic epitope libraries and studying polyclonal TCR repertoires covering highly polymorphic HLA-I allotypes and oligomorphic nonclassical molecules.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Engineering, Biomedical
Xiaodi Li, Pengfei Yuan, Haiyuan Yang, Xiaoqing Zong, Caiqi Yang, Xinjie Chen, Yuchao Li, Xiaodie Yan, Yaoqi Wen, Tianci Zhu, Qian Zhang, Wei Xue, Jian Dai
Summary: A novel virus-like nanotherapeutic (siRNA@HCM) is developed to enhance the antitumor immune response. It achieves this by improving tumor intrinsic MHC-I antigen presentation and promoting dendritic cell maturation. The use of siRNA@HCM also turns cold tumors into hot tumors and enhances the therapeutic effect of programmed death-1 inhibitor.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Immunology
Karthik Dhatchinamoorthy, Jeff D. Colbert, Kenneth L. Rock
Summary: Loss of MHC I antigen presentation is common in many cancers, which may impair immune responses and affect the efficacy of immunotherapy. Studies have discussed underlying mechanisms through which some cancers evade immune killing by shutting down the MHC I pathway, and proposed potential strategies to overcome this limitation.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Carolina Johnstone, Elena Chaves-Pozo
Summary: This review focuses on the essential components of antigen processing and presentation (APP) in teleost fish and the increasing evidence related to the modulation of APP and autophagy during pathogen infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Freidrich M. Cruz, Amanda Chan, Kenneth L. Rock
Summary: Phagocytes, particularly dendritic cells, play a crucial role in reporting antigen information to CD8 T cells through a process called cross-presentation. This process allows CD8 T cells to detect and eliminate abnormal cells. Phagosomes are key hubs in the cross-presentation process, where antigens can be cross-presented through different pathways. Understanding the molecular components and mechanisms involved in these processes is still an ongoing research area.
SEMINARS IN IMMUNOLOGY
(2023)
Article
Immunology
Jimena Alvarez Freile, Yuzhu Qi, Lisa Jacob, Maria Franceskin Lobo, Harm Jan Lourens, Gerwin Huls, Edwin Bremer
Summary: Investigations into the strength of antigen-specific responses in vitro are crucial for early-phase research of immunotherapeutic approaches. This study presents a rapid luminescence-based method using HPV16 E7(11-20) peptide as a model antigen to evaluate MHC-dependent antigen-specific T cell responses in vitro. The method has important implications for assessing the impact of tumor microenvironment and immune checkpoint inhibitors, as well as accelerating the development of novel immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biology
Julia Bauza-Martinez, Albert J. R. Heck, Wei Wu
Summary: Bauza-Martinez et al. analyzed the HLA-I peptide ligandome in extracellular vesicles (EVs) from an EBV-immortalized B cell line, JY, and compare HLA-I-binding peptides recovered from EVs to those recovered from whole-cells. The authors report an enrichment for HLA-B-binding peptides and for post-translationally modified cysteinylated peptides in EVs as compared to whole-cells.
COMMUNICATIONS BIOLOGY
(2021)
Article
Rheumatology
Antony Nicodemus Antoniou, Lzabela Lenart, Janos Kriston-Vizi, Takao Lwawaki, Mark Turmaine, Kirsty McHugh, Sadfer Ali, Neil Blake, Paul Bowness, Mona Bajaj-Elliott, Keith Gould, Darren Nesbeth, Simon J. Powis
ANNALS OF THE RHEUMATIC DISEASES
(2019)
Article
Multidisciplinary Sciences
Alasdair H. Fikouras, Marcel Schubert, Markus Karl, Jothi D. Kumar, Simon J. Powis, Andrea Di Falco, Malte C. Gather
NATURE COMMUNICATIONS
(2018)
Article
Optics
Roopam K. Gupta, Mingzhou Chen, Graeme P. A. Malcolm, Nils Hempler, Kishan Dholakia, Simon J. Powis
Article
Optics
Roopam K. Gupta, Graham D. Bruce, Simon J. Powis, Kishan Dholakia
LASER & PHOTONICS REVIEWS
(2020)
Article
Biology
Jacob Beal, Natalie G. Farny, Traci Haddock-Angelli, Vinoo Selvarajah, Geoff S. Baldwin, Russell Buckley-Taylor, Markus Gershater, Daisuke Kiga, John Marken, Vishal Sanchania, Abigail Sison, Christopher T. Workman
COMMUNICATIONS BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Andrew T. Meek, Nils M. Kronenberg, Andrew Morton, Philipp Liehm, Jan Murawski, Eleni Dalaka, Jonathan H. Booth, Simon J. Powis, Malte C. Gather
Summary: The authors introduce an interference-based method illuminated by two rapidly alternating wavelengths for real-time mapping of nanoscale forces with sub-second mechanical fluctuations. This technique allows monitoring of cellular forces with high throughput and excellent temporal resolution, providing a new approach for studying dynamic processes in mechanobiology.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Caitlin Boyne, Debra Lennox, Olivia Beech, Simon J. Powis, Pankaj Kumar
Summary: The HLA-I system plays a crucial role in activating cytotoxic lymphocytes and immune attack against pathogen-infected and cancer cells. While the ligandome of cancer cell surface HLA class I molecules has been extensively studied, the ligandome of extracellular vesicles remains relatively undefined. Understanding this could advance immune-based therapeutic approaches for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Pankaj Kumar, Caitlin Boyne, Sydney Brown, Ayesha Qureshi, Peter Thorpe, Silvia A. Synowsky, Sally Shirran, Simon J. Powis
Summary: This study reveals the HLA-I ligandome in extracellular vesicles (EVs) from multiple cancer cell lines and identifies tumor-associated antigenic peptides. These findings contribute to a better understanding of tumor immune responses.
Article
Immunology
Izabela Lenart, Linh-Huyen Truong, Dinh Dung Nguyen, Olga Rasiukiene, Edward Tsao, Jonathan Armstrong, Pankaj Kumar, Kirsty McHugh, Branca I. Pereira, Balraj S. Maan, Malgorzata A. Garstka, Paul Bowness, Neil Blake, Simon J. Powis, Keith Gould, Darren Nesbeth, Antony N. Antoniou
Summary: Peptide-loaded Major Histocompatibility Complex (pMHC) class I molecules can be expressed in a single chain trimeric (SCT) format. Certain MHC class I molecules, including HLA-B27 HC, tend to form aggregates. The folding and dimerization of the single chain complex are determined by specific regions of the HLA-B27 HC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Janos Kriston-Vizi, Izabela Lenart, Takao Iwawaki, Keith Gould, Darren Nesbeth, Simon J. Powis, Antony N. Antoniou
Summary: The study found that Salmonella infections induce and require unfolded protein response pathways for intracellular replication, and infections with Salmonella may be associated with the human leukocyte antigen HLA-B * 27:05. Expression of HLA-B * 27:05 alters the cellular distribution of Salmonella within epithelial cells, and is associated with the Salmonella's requirements for UPR induction and endoplasmic reticulum membrane expansion.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Review
Medicine, General & Internal
Milan Terzic, Dinara Makhadiyeva, Jovan Bila, Mladen Andjic, Jelena Dotlic, Gulzhanat Aimagambetova, Antonio Sarria-Santamera, Antonio Simone Lagana, Vito Chiantera, Ivana Vukovic, Dusica Kocijancic Belovic, Slavica Aksam, Gauri Bapayeva, Sanja Terzic
Summary: Cervical cancer is a major cause of cancer-related death in women of reproductive age. Fertility-sparing procedures such as LEEP, cold-knife conization, and trachelectomy can be considered safe and effective treatments for preserving reproductive potential. However, careful monitoring of pregnancies obtained after fertility-preserving procedures is recommended, as these patients are at a higher risk.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Optics
Roopam K. Gupta, Nils Hempler, Graeme P. A. Malcolm, Kishan Dholakia, Simon J. Powis
Summary: T cells of the adaptive immune system are effectively protected against pathogenic challenges. Traditional labelling methods are time-consuming and expensive, but digital holographic microscopy with deep learning proves to be a faster and more cost-effective alternative. The combination of DHM and deep learning achieves high throughput and accuracy in classifying CD4+ and CD8+ T cell subsets.
Meeting Abstract
Obstetrics & Gynecology
D. Makhadiyeva, A. Ibragimov
HUMAN REPRODUCTION
(2020)
Article
Biology
Antony N. Antoniou, Simon J. Powis, Janos Kriston-Vizi
BMC RESEARCH NOTES
(2019)
Article
Multidisciplinary Sciences
Swati Arya, Dagmara Wiatrek-Moumoulidis, Silvia A. Synowsky, Sally L. Shirran, Catherine H. Botting, Simon J. Powis, Alan J. Stewart
SCIENTIFIC REPORTS
(2019)