Preparation of NiFe2O4 architectures for affinity separation of histidine-tagged proteins

NiFe2O4 architectures were synthesized by a hydrothermal method. NiFe2O4 architectures consist of radial fibers with 30 nm in diameters and 1 gm in lengths. These architectures possess an excellent magnetic response and have Brunauer-Emmett-Teller (BET) surface area of 73.1 m(2)/g. Due to their high Ni2+ density, these architectures can be used to magnetically separate histidine-tagged (His-tagged) proteins directly from the mixture of lysed cells. The results show that the architectures present negligible nonspecific adsorption and high protein binding activity with the max value of 120.1 mg/g. (C) 2015 Elsevier B.V. All rights reserved.