Review
Microbiology
Colin W. K. Rosman, Jan Maarten van Dijl, Jelmer Sjollema
Summary: This review discusses the factors leading to sub-optimal clearance of Staphylococcus aureus bacteria involved in BAIs, as well as how experimental interventions can impact infectious mechanisms. The focus of treatment and intervention should be on the first week after implantation and infection, as this period is crucial for the establishment of S. aureus biofilm.
CRITICAL REVIEWS IN MICROBIOLOGY
(2022)
Article
Microbiology
Anais Lemaire, Jennifer Varin-Simon, Fabien Lamret, Marie Dubus, Halima Kerdjoudj, Frederic Velard, Sophie C. Gangloff, Fany Reffuveille
Summary: Infections are a critical issue during the development of biomimetic materials for bone regeneration. Calcium phosphate and collagen substrates may favor bacterial adhesion. Staphylococcus aureus possesses adhesins that allow binding to these substrates, leading to the development of biofilms highly tolerant to immune system attacks or antibiotic treatments. Therefore, choosing the right material for bone substitutes is crucial to prevent bacterial adhesion and reduce the risk of bone and joint infections.
Article
Immunology
Abigail M. Forson, Colin W. K. Rosman, Theo G. van Kooten, Henny C. van der Mei, Jelmer Sjollema
Summary: Advancements in contemporary medicine have led to an increasing life expectancy and the broader use of biomaterial implants. However, the risk of biomaterial-associated infections is also increasing. Staphylococcus aureus is a common cause of these infections and is known for its ability to form biofilms. This study found that by secreting micrococcal nuclease, S. aureus can evade the immune response and form persistent infections. The production of micrococcal nuclease also stimulates biofilm formation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Abigail M. Forson, Colin W. K. Rosman, Theo G. van Kooten, Henny C. van der Mei, Jelmer Sjollema
Summary: Advancements in contemporary medicine have led to an increasing life expectancy and the broadened application of biomaterial implants. However, the risk of infection during implant procedures has led to a rise in biomaterial-associated infections. Staphylococcus aureus is a major cause of such infections, and its ability to produce micrococcal nuclease allows it to evade the immune response and form biofilms, leading to persistent infections. This study highlights the importance of targeting micrococcal nuclease production as a novel strategy in preventing biomaterial-associated infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Surgery
Mathilde Puges, Xavier Berard, Sebastien Vilain, Sabine Pereyre, Isabelle Svahn, Caroline Caradu, Fatima Mzali, Charles Cazanave
Summary: This study evaluated the adhesion and biofilm formation of Staphylococcus aureus on vascular grafts. Results showed that triclosan-impregnated grafts were the most effective in preventing adhesion and biofilm formation, while silver impregnation and collagen coating were less effective than expected.
EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY
(2023)
Review
Biochemistry & Molecular Biology
Giampiero Pietrocola, Davide Campoccia, Chiara Motta, Lucio Montanaro, Carla Renata Arciola, Pietro Speziale
Summary: The use of indwelling medical devices has increased in recent years, improving the quality of life for patients. However, implant-associated infections, especially in orthopedic surgery, remain a common and serious complication. Staphylococcus aureus is the main cause of biomaterial-centered infections, forming biofilms that enable the bacteria to evade antimicrobial activity and the immune response. Understanding the mechanisms of biofilm formation and developing innovative anti-biofilm approaches are crucial for treating biomaterial-associated infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Fengjia Chen, Jing Zhang, Hyun Jung Ji, Min-Kyu Kim, Kyoung Whun Kim, Jong-Il Choi, Seung Hyun Han, Sangyong Lim, Ho Seong Seo, Ki Bum Ahn
Summary: Deinococcus radiodurans is a highly resistant bacterium with cellular constituents, such as DeinoPol, playing a key role in inhibiting Staphylococcus aureus colonization and biofilm formation. DeinoPol interferes with S. aureus biofilm formation by disrupting production of poly-N-acetylglucosamine, suggesting its potential application in preventing/treating S. aureus biofilm-related infections or inflammatory diseases.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Li Shen, Jiao Zhang, Yao Chen, Lulin Rao, Xinyi Wang, Huilin Zhao, Bingjie Wang, Yanghua Xiao, Jingyi Yu, Yanlei Xu, Junhong Shi, Weihua Han, Zengqiang Song, Fangyou Yu
Summary: In this study, a novel small-molecule compound CY-158-11 was synthesized and found to have inhibitory effects on the biofilm formation of Staphylococcus aureus. The subinhibitory concentration of CY-158-11 did not affect bacterial growth and showed no toxicity to cells. The compound effectively prevented initial attachment, repressed PIA production, and suppressed genes involved in biofilm formation. Overall, CY-158-11 inhibits biofilm formation in S. aureus by inhibiting cell adhesion and the expression of icaA gene related to PIA production.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Amelia Staats, Peter W. Burback, Mostafa Eltobgy, Dana M. Parker, Amal O. Amer, Daniel J. Wozniak, Shu-Hua Wang, Kurt B. Stevenson, Kenneth L. Urish, Paul Stoodley
Summary: This study aimed to investigate the strain dependency of synovial fluid-induced aggregation in S. aureus isolates and its correlation with surface-associated biofilm formation. It was found that different strains exhibited varying degrees of aggregation in response to synovial fluid and PJIs isolates aggregated more significantly than those from bloodstream infections. Surprisingly, there was no correlation between the degree of synovial fluid-induced aggregation and quantity of surface-associated biofilm.
MICROBIOLOGY SPECTRUM
(2021)
Article
Plant Sciences
Maya Rima, Asma Chbani, Christine Roques, Fatima El Garah
Summary: This study explores the potential antibiofilm activity of extracts derived from three types of algae against Staphylococcus aureus biofilm. Four extracts were found to significantly inhibit biofilm formation, and one extract demonstrated a significant reduction in surface hydrophobicity of S. aureus.
Article
Microbiology
Jiao Zhang, Li Shen, Peiyao Zhou, Shuying Chen, Bingjie Wang, Cailin Wan, Weihua Han, Lulin Rao, Huilin Zhao, Xinyi Wang, Chunyang Wu, Junhong Shi, Yanghua Xiao, Zengqiang Song, Fangyou Yu, Chunchan Lin
Summary: This study demonstrates that a small-molecule compound S-342-3 effectively inhibits the biofilm formation of Staphylococcus aureus. It reduces biofilm mass and suppresses the production of polysaccharide intercellular adhesin, thus preventing cell adhesion. Importantly, S-342-3 shows excellent biosafety without toxic effects on cells and animals.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Khulood Fahad Alabbosh, Tarek Zmantar, Abdulrahman S. Bazaid, Mejdi Snoussi, Emira Noumi
Summary: This study investigated the adhesive properties and antibiotic resistance of clinical S. aureus isolated from Hail Hospital Province, Kingdom of Saudi Arabia using molecular approaches. The findings suggest that antibiotic resistance and adhesion play important roles in the pathogenesis of clinical S. aureus isolates.
Article
Infectious Diseases
Cristina El Haj, Eva Benavent, Yanik Sierra, Laura Soldevila, Raul Rigo-Bonnin, Benjamin Torrejon, Joan Gomez-Junyent, Irantzu Rossello, Oscar Murillo
Summary: The anti-biofilm efficacy of dalbavancin (DAL) was significantly improved by adding rifampicin (RIF) in the treatment of biofilm-embedded methicillin-resistant Staphylococcus aureus (MRSA). However, the development of RIF resistance in all combination therapies decreased their effectiveness over time.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Review
Immunology
Kara R. Eichelberger, James E. Cassat
Summary: Successful pathogens such as Staphylococcus aureus and Candida albicans exhibit metabolic flexibility to adapt to various host niches. Co-infection of these two microorganisms alters microbial metabolism, enhances virulence, and leads to the formation of polymicrobial biofilms. The metabolic changes induced during co-infection impact interactions with host immune cells, resulting in greater microbial survival and immune evasion.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Youliang Ren, Jin Yang, Jinghui Zhang, Xiao Yang, Lei Shi, Dajing Guo, Yuanyi Zheng, Haitao Ran, Zhongliang Deng, Lei Chu
Summary: The study found that PMMA+2% Fe3O4+AMF has promising therapeutic potential for implant-associated bone infection (SIABI). It provides excellent mechanical support and effectively eradicates residual pathogenic bacteria in bone infected lesions.
ADVANCES IN NANO RESEARCH
(2022)
Article
Chemistry, Physical
Robert Baumann, Yasmine Bouraoui, Uwe Teicher, Erik Selbmann, Steffen Ihlenfeldt, Andres Fabian Lasagni
Summary: In the context of societal development, manufacturers in the metal industries are increasingly concerned about sustainability. This study explores the use of laser interference patterning to texture cutting tools, aiming to reduce energy consumption and material waste. The textured tools showed improved tribological performance in turning experiments, with a 17% decrease in machining forces and a 31% decrease in surface roughness of the aluminum work piece compared to untreated tools.
Article
Materials Science, Multidisciplinary
Felix Bouchard, Marcos Soldera, Andres Fabian Lasagni
Summary: In this study, a cost-effective strategy is demonstrated for fabricating micro-structured PMMA diffusers for white light sources. Different laser-based processes are combined to fabricate periodic patterns on stainless steel surfaces and transfer them to PMMA surfaces. The relationship between surface topography and white light scattering behavior is investigated, and the hierarchical structures can significantly increase haze while maintaining high transmittance.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Lukas Krause, Katarzyna Skibinska, Hannes Rox, Robert Baumann, Mateusz M. Marzec, Xuegeng Yang, Gerd Mutschke, Piotr Zabinski, Andres Fabian Lasagni, Kerstin Eckert
Summary: Emerging manufacturing technologies allow for the design of electrocatalyst morphology on the nanoscale to improve efficiency in electrolysis processes. This study investigates the impact of electrode-attached hydrogen bubbles on electrode performance, depending on surface morphology and wettability. Nickel-based electrocatalysts with hydrophilic and hydrophobic nanostructures are manufactured and their surface properties are characterized. Despite a larger electrochemically active surface area, electrochemical analysis shows that samples with more pronounced hydrophobic properties perform worse at industrially relevant current densities. High-speed imaging reveals larger bubble detachment radii with higher hydrophobicity, indicating that gas blockage outweighs the benefits of nanostructuring. Additionally, a slight decrease in bubble size of 7.5% is observed with an increase in current density in 1 M KOH.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Materials Science, Multidisciplinary
Kangfa Deng, Qihao Zhang, Yangxi Fu, Andres Fabian Lasagni, Heiko Reith, Kornelius Nielsch
Summary: This article presents a novel fabrication technique named PowderMEMS for high-performance, low-cost TE films and micro-patterns. The TE film is composed of agglomerated micro-sized N-type Bi2Te2.5Se0.5 powders with a molten binder of bismuth (Bi). The influence of key process parameters on TE performance is investigated, and the TE film exhibits a maximum power factor of 1.7 mW m-1K-2 at room temperature, the highest value reported so far.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Herman Heffner, Marcos Soldera, Fabian Raenke, Andres Fabian Lasagni
Summary: The direct laser interference patterning (DLIP) method is used to control the surface morphology, optical, and electrical properties of fluorine-doped tin oxide (FTO) in order to optimize the efficiency of solar cells. The study reveals the presence of periodic line-like microstructures on the FTO surface, with a period of 3.0 μm and heights ranging from 20 to 185 nm. The generated micro- and nanostructures result in a significant increase in optical transmittance, suggesting that DLIP is a convenient technique for structuring electrodes for highly efficient optoelectronic devices.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Nikolai Schroeder, Christoph Fischer, Marcos Soldera, Bogdan Voisiat, Andres Fabian Lasagni
Summary: Process monitoring is a crucial strategy in laser-based manufacturing to improve reliability, efficiency, and economic profit. Laser surface texturing workstations utilize monitoring techniques to analyze surface topography and enhance the quality of produced workpieces. In this study, dot-like periodic surface structures were fabricated on stainless steel samples using direct laser interference patterning (DLIP). A scatterometry-based measurement device was used to indirectly determine the depth and period of the topography. The estimated average depth and spatial period of the dot-like structures had relative errors below 15% and 2% respectively. This new process monitoring approach greatly enhances quality assurance in DLIP processing.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Bruno Henriques, Douglas Fabris, Bogdan Voisiat, Andres Fabian Lasagni
Summary: In this study, the influence of laser processing parameters on the production of functional multi-scale PEEK surfaces using Direct Laser Interference Patterning (DLIP) technology was investigated. An infrared ultra-short pulsed laser source and a two-beam interference setup were utilized. The fabricated structures were characterized by Scanning Electron Microscopy and Confocal Microscopy, revealing the formation of line-like main structures with a periodic spatial repetition of 5 μm and secondary structures perpendicular to the main structures with a periodicity of approximately 1 μm (LIPSS) at DLIP maxima positions.
Article
Nanoscience & Nanotechnology
Lucinda E. Mulko, Emma A. Cuello, Robert Baumann, Anthony R. Ramuglia, Inez M. Weidinger, Diego F. Acevedo, Cesar A. Barbero, Maria Molina, Andres Fabian Lasagni
Summary: In this study, a polyacrylamide graphene oxide hydrogel composite (GO@pAAm) was photo-converted and structured by ultra-short laser irradiation using a direct laser writing (DLW) approach. The laser structure generated surface foaming and patterning, enhanced selective water-swelling microdomains, and effective photo-reduction of graphene oxide. The reduction of graphene oxide was evidenced by changes in the Raman spectroscopy signals. Macroscopically, the conductivity of the material increased, suggesting a reduction in sheet resistance.
Article
Food Science & Technology
Liliana Lai, Maite Villanueva, Ane Muruzabal-Galarza, Ana Beatriz Fernandez, Argine Unzue, Alejandro Toledo-Arana, Primitivo Caballero, Carlos J. Caballero
Summary: Aedes albopictus, a mosquito species from Southeast Asia, has rapidly spread to temperate regions, making them vulnerable to diseases like dengue, yellow fever, zika, and chikungunya. The mosquito has developed resistance to commonly used insecticides, necessitating the search for new toxins to control their population.
Article
Chemistry, Multidisciplinary
Felix Bouchard, Marcos Soldera, Andres Fabian Lasagni
Summary: This study investigates the wetting behavior of microstructured polyethylene terephthalate (PET) foils produced by plate-to-plate hot embossing for polar and nonpolar liquids. Stainless steel plates with single-scaled and hierarchical textures created by direct laser writing and two-beam direct laser interference patterning are used as stamps for the embossing step. The imprinted microstructures, consisting of pillar- and line-like textures, exhibit increased water contact angles and superoleophilic behavior. Time-resolved measurements reveal rapid oil droplet spreading on the hierarchical textures up to velocities of 1.4 mm(2) s(-1). This functionalization of PET foils opens up new possibilities for various industrial applications such as using oil-based paints instead of solvent-based ones, improving lubricant distribution in mechanical components, or oil-water separation in maritime environments.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Chemistry, Analytical
Frederic Schell, Richard Chukwudi Okafor, Tobias Steege, Sabri Alamri, Savan Ghevariya, Christoph Zwahr, Andres F. Lasagni
Summary: This study presents a microscopic approach to increasing surface area by using periodic surface structures. The experiments show that microstructures with a periodic distance of 8.5 μm fabricated using nanosecond-pulsed infrared laser can significantly increase the surface area and improve heat dissipation efficiency.
Article
Materials Science, Multidisciplinary
Bruno Henriques, Douglas Fabris, Bogdan Voisiat, Andres Fabian Lasagni
Summary: In this study, micrometric structures were fabricated on the surface of Cobalt-Chromium-Molybdenum and AZ91D magnesium alloys using the technique of Direct Laser Interference Patterning (DLIP). The surface topography, morphology, and chemical modifications were analyzed using Confocal Microscopy, Scanning Electron Microscopy, and Energy Dispersive Spectroscopy (EDS), respectively. Varying laser fluence and pulse overlap showed their influence on the final structure, with deeper structures achieved for higher energy levels. The results provide new perspectives on the fabrication of microtextures on the surface of CoCrMo and AZ91D using DLIP.
Article
Microbiology
Beatriz Rapun-Araiz, Ioritz Sorzabal-Bellido, Javier Asensio-Lopez, Maria Lazaro-Diez, Mikel Ariz, Carlos Sobejano de la Merced, Begona Euba, Ariadna Fernandez-Calvet, Ivan Cortes-Dominguez, Saioa Burgui, Alejandro Toledo-Arana, Carlos Ortiz-de-Solorzano, Junkal Garmendia
Summary: Standardized clinical procedures for antibiotic administration often fail to optimize treatment for respiratory pathogens due to polyclonal infections. This study presents a panel of plasmid tools for labeling and visualizing H. influenzae polyclonal infections, and demonstrates their usefulness in analyzing mixed biofilm growth, antibiotic efficacy, and bacterial co-infection dynamics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Carmen Gomez-Arrebola, Sara B. Hernandez, Elizabeth J. Culp, Gerard D. Wright, Cristina Solano, Felipe Cava, Inigo Lasa
Summary: The overuse of antibiotics has led to the emergence of antimicrobial resistance, stimulating the search for novel antibiotics. This study investigates the role of the VraSR sensory system in Staphylococcus aureus susceptibility to Complestatin and Corbomycin, revealing the important role of spdC and sagB genes in antibiotic resistance.
MICROBIOLOGY SPECTRUM
(2023)
