Article
Immunology
Basudev Paudyal, Adam McNee, Pramila Rijal, B. Veronica Carr, Alejandro Nunez, John McCauley, Rodney S. Daniels, Alain R. Townsend, John A. Hammond, Elma Tchilian
Summary: Prophylactic intravenous administration of specific monoclonal antibodies in pigs showed significant reduction in lung pathology and virus loads, but not consistent reduction in nasal shedding. The effect on lung pathology and virus load is consistent across different doses, suggesting that lower doses may be effective in reducing disease severity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nohara Goto, Hiroyuki Suzuki, Tomohiro Tanaka, Teizo Asano, Mika K. Kaneko, Yukinari Kato
Summary: CD44 is a cell surface glycoprotein involved in various cellular functions. The established antibody C(44)Mab-46 is important for cancer diagnosis and therapy by detecting CD44 in different applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hiroyuki Suzuki, Kaishi Kitamura, Nohara Goto, Kenichiro Ishikawa, Tsunenori Ouchida, Tomohiro Tanaka, Mika K. Kaneko, Yukinari Kato
Summary: CD44 promotes tumor progression by recruiting growth factors and acquiring stemness, invasiveness, and drug resistance. We have established various anti-CD44s and anti-CD44v monoclonal antibodies through immunization and in this study, we developed C(44)Mab-6, which could detect CD44v3 in various experiments and has potential applications in tumor diagnosis and therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ryo Ejima, Hiroyuki Suzuki, Tomohiro Tanaka, Teizo Asano, Mika K. Kaneko, Yukinari Kato
Summary: CD44 is a cell surface glycoprotein with isoforms produced by alternative splicing. CD44 variant exon-containing isoforms (CD44v) are overexpressed in carcinomas, and CD44v6 predicts poor prognosis in colorectal cancer patients. CD44v6 plays important roles in CRC adhesion, proliferation, stemness, invasiveness, and chemoresistance. In this study, anti-CD44 monoclonal antibodies (mAbs) were developed and characterized for detecting CD44v6 in various applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hiroyuki Suzuki, Kazuki Ozawa, Tomohiro Tanaka, Mika K. Kaneko, Yukinari Kato
Summary: CD44 is investigated as a cancer stem cell marker in tumor progression. Different splicing variants are overexpressed in many cancers and play critical roles in tumor metastasis, acquisition of stem cell properties, and resistance to treatments. Therefore, understanding the functions and distribution of each CD44 variant is important for novel tumor diagnosis and therapy.
Article
Chemistry, Multidisciplinary
Rinie Bajracharya, Esteban Cruz, Jurgen Gotz, Rebecca M. Nisbet
Summary: Tau-specific immunotherapy is a promising treatment strategy for Alzheimer's disease. However, the blood-brain barrier poses a challenge for effective delivery of tau-specific antibodies. Previous research has shown that low-intensity scanning ultrasound combined with microbubbles can increase antibody passage into the brain. This study aimed to assess the therapeutic response of ultrasound-mediated delivery of tau-specific antibodies.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Biochemistry & Molecular Biology
Mayuki Tawara, Hiroyuki Suzuki, Nohara Goto, Tomohiro Tanaka, Mika K. K. Kaneko, Yukinari Kato
Summary: Researchers developed a monoclonal antibody, C(44)Mab-1, which can sensitively and specifically detect the expression of CD44v9 in cancer. This antibody is useful for flow cytometry, western blotting, and immunohistochemistry to detect CD44v9 in colorectal cancer.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eszter Kozak, Bence Szikora, Attila Ilias, Peter K. Jani, Zoltan Hegyi, Zsolt Matula, Dora Dedinszki, Natalia Tokesi, Krisztina Fulop, Viola Pomozi, Gyorgy Varady, Eva Bakos, Gabor E. Tusnady, Imre Kacskovics, Andras Varadi
Summary: Mutations in the ABCC6 gene lead to calcification diseases, and researchers have developed a monoclonal antibody, mEChC6, that recognizes an extracellular epitope of hABCC6 with specificity, verifying its binding to intact hepatocytes.
Article
Respiratory System
Cecilia M. Prele, Tylah Miles, David R. Pearce, Robert J. O'Donoghue, Chris Grainge, Lucy Barrett, Kimberly Birnie, Andrew D. Lucas, Svetlana Baltic, Matthias Ernst, Catherine Rinaldi, Geoffrey J. Laurent, Darryl A. Knight, Mark Fear, Gerard Hoyne, Robin J. McAnulty, Steven E. Mutsaers
Summary: This study finds that plasma cells play an important role in the development and progression of pulmonary fibrosis, and inhibiting B-cell function does not reduce fibrosis. This is significant for understanding the pathological mechanism of pulmonary fibrosis and developing anti-fibrotic treatment strategies.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Chemistry, Analytical
Yanbo Wang, Mingyang Wang, Haipeng Yu, Ge Wang, Pengxin Ma, Shuang Pang, Yiming Jiao, Aihua Liu
Summary: PSA is an important index for prostate cancer diagnosis and treatment. In this study, a biopanning method for PSA was established using a pIII phage display library. Two phage monoclonal antibodies specific to PSA were obtained, and an ELISA system was constructed for detecting tPSA with high accuracy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Immunology
Yingfen Wen, Wenjing Guo, Yuyi Min, Kexin Zhong, Xulei Zhang, Xiaomin Xing, Yuwei Tong, Yuejun Pan, Wenxin Hong, Weiping Cai, Lei Yu
Summary: Researchers isolated five monoclonal antibodies (mAbs) specific to the nucleocapsid (N) protein of SARS-CoV-2 from memory B cells in convalescent COVID-19 patients. These mAbs targeted either the C-terminal domain (CTD) or the N-terminal domain (NTD) of the N protein and showed cross-reactivity to the N protein of SARS-CoV. These patient-derived mAbs have the potential to be used for developing next-generation COVID-19 At-Home Test kits.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shikha Grover, Thao Pham, Anna Jones, Cristina Sinobas-Pereira, Marina Villoch Diaz Maurino, Evan C. C. Garrad, Nyasha J. J. Makoni, Antanisha Parks, Ryan J. J. Domalewski, Gabriel Riggio, Hannah An, Kunhua Chen, Michael R. R. Nichols
Summary: This study developed and characterized a new class of monoclonal antibodies (mAbSL) that selectively target A beta protofibrils, inhibiting A beta monomer aggregation and altering its dynamics.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Biology
Isabel C. Hutchison, Stefania Pezzoli, Maria-Efstratia Tsimpanouli, Mahmoud E. A. Abdellahi, Penelope A. Lewis
Summary: Research suggests that targeted memory reactivation during sleep, especially in REM stage, can help decrease subjective arousal responses associated with emotional memories. This supports the idea that emotional memory reactivation during REM sleep underlies sleep-dependent habituation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Avik Banerjee, Jiachen Huang, Scott A. Rush, Jackelyn Murray, Aaron D. Gingerich, Fredejah Royer, Ching-Lin Hsieh, Ralph A. Tripp, Jason S. McLellan, Jarrod J. Mousa
Summary: This study identified immunodominant antigenic epitopes on the hMPV F protein and identified a monoclonal antibody (mAb) therapy for hMPV F disease prevention and treatment. Additionally, a prefusion-specific epitope on the hMPV F protein was discovered.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Georgia K. Dwyer, Rick J. Stoffels, Ewen Silvester, Gavin N. Rees
Summary: In nutritional ecology, the goal is to maximize consumer fitness through diet. The Selectively Consuming prey to match the Intake Target (SCIT) hypothesis suggests that consumers have evolved to selectively consume prey to meet their nutritional needs. This phenomenon has been observed in some herbivores and omnivores, and is also likely to occur in carnivores despite the homogeneity of their prey's nutrient composition.
SCIENTIFIC REPORTS
(2023)
