Article
Biochemistry & Molecular Biology
Edgar Del Llano, Aurore Perrin, Frederic Morel, Francoise Devillard, Radu Harbuz, Veronique Satre, Florence Amblard, Marie Bidart, Sylviane Hennebicq, Sophie Brouillet, Pierre F. F. Ray, Charles Coutton, Guillaume Martinez
Summary: RT carriers face higher risks of infertility, miscarriage, and offspring with anomalies and developmental delay. Prenatal diagnosis or preimplantation genetic diagnosis can reduce these risks. However, a recent study questions the usefulness of spermFISH, a commonly used method, for investigating the meiotic segregation of RT carriers. A comprehensive analysis of 41 RT carriers confirms that acrocentric chromosomes lead to more unbalanced gametes, suggesting routine implementation of spermFISH is not beneficial for these patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Leah F. Rosin, Jose Gil, Ines A. Drinnenberg, Elissa P. Lei
Summary: Accurate chromosome segregation is crucial for reproductive success. The use of Oligopaints in the silkworm Bombyx mori has provided new insights into mechanisms of homolog pairing during meiosis, revealing asynchronous pairing, transcription-biased pairing in males, and similarities in bivalent formation with C. elegans. Furthermore, the study suggests that microtubule recruitment may be dependent on kinetochore proteins but independent of centromeres, and that telomeres play a central role in regulating chromosome interactions during sperm and egg development.
Article
Reproductive Biology
D. J. Galindo, M. Vozdova, S. Kubickova, H. Cernohorska, A. M. Bernegossi, D. Kadlcikova, J. Rubes, J. M. B. Duarte
Summary: The study used FISH with bovine WCP and BAC probes to analyze chromosome rearrangements and gametic segregation in brown brocket deer, finding a slight to moderate increase in the rates of unbalanced meiotic segregation products in individuals heterozygous for RT and RT/PAIs, but without statistical significance.
Article
Genetics & Heredity
Shuo Zhang, Caixia Lei, Junping Wu, Jing Zhou, Min Xiao, Saijuan Zhu, Yanping Xi, Jing Fu, Yijuan Sun, Congjian Xu, Xiaoxi Sun
Summary: Our study demonstrated that meiotic segregation heterogeneity of trivalent structure is associated with the carrier's gender and translocation type, and it is independent of carrier's age. ICE phenomenon exists during meiosis and then increases the frequencies of additional chromosome abnormalities.
FRONTIERS IN GENETICS
(2021)
Article
Agriculture, Dairy & Animal Science
Sara Ruiz-Diaz, Rosane Mazzarella, Paula Navarrete-Lopez, Raul Fernandez-Gonzalez, Celia de Frutos, Maria Maroto, Claudia Cucala, Paula Beltran-Brena, Marta Lombo, Dimitrios Rizos, Alfonso Gutierrez-Adan
Summary: This study aimed to analyze if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by ICSI. The results showed that thermotactic sperm selection led to higher DNA integrity, small and elongated head size parameters, and a different sperm subpopulation structure.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Neil Hattersley, Aleesa J. Schlientz, Bram Prevo, Karen Oegema, Arshad Desai
Summary: The nucleoporin MEL-28 and CENP-CHCP-4 act in parallel to recruit outer kinetochore components to oocyte meiotic chromosomes, and they independently link chromosomes to spindle microtubules.
Article
Obstetrics & Gynecology
Liuyan Lin, Xueyao Chen, Jing Wang, Rong Li, Chenhui Ding, Bing Cai, Canquan Zhou, Yanwen Xu
Summary: The sex of reciprocal translocation carriers affects meiotic segregation patterns, but has no effect on the stability of non-translocated chromosomes during meiosis.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Fisheries
Henrik Jeuthe, Christos Palaiokostas, Anders Johannisson
Summary: The paternal factor in Arctic charr broodstock is more closely related to embryo survival than fertilization rates, with high levels of DNA fragmentation in sperm but good membrane integrity. Fertilization rates and proportion of eyed eggs show variation and correlation, but the main loss is due to embryo mortality. DNA fragmentation and membrane integrity in sperm did not show correlation with the resulting reproductive outcome of Arctic charr sires.
Article
Andrology
Riffat Bibi, Sarwat Jahan, Suhail Razak, Mohammad Eid Hammadeh, Ali Almajwal, Houda Amor
Summary: This study aims to identify functional tests in semen that are associated with DNA damage and chromatin maturity, in order to predict outcomes in assisted reproduction. The results suggest that male factor infertility is associated with higher levels of DNA fragmentation and chromatin decondensation in sperm. Additionally, there is a positive correlation between fertilization rate and live birth rate with sperm concentration, motility, vitality, and normal morphology, while there is a negative correlation between sperm DNA fragmentation index and chromatin maturity. However, no correlation was found between embryo aneuploidy and sperm DNA fragmentation or chromatin maturity.
Article
Cell Biology
Foyez Shams, Darryl D'Souza, Tariq Ezaz
Summary: In a study conducted on a representative sample of 94 boars from a commercial nucleus herd in Australia, it was found that 6.38% of the samples had novel chromosome rearrangements associated with hypoprolificacy. These rearrangements, including a previously detected translocation between chromosomes 3 and 16, were identified through standard and molecular cytogenetic analyses. Further research and routine monitoring will be essential to prevent the propagation of reciprocal translocations in the Australian pig population.
Article
Genetics & Heredity
Caixia Lei, Shuo Zhang, Saijuan Zhu, Junping Wu, Min Xiao, Jing Zhou, Jing Fu, Yijuan Sun, Congjian Xu, Xiaoxi Sun
Summary: The study evaluated the chromosome contents of selected sperm for ICSI from male carriers of reciprocal translocation, finding higher rates of normal/balanced chromosome contents compared to unselected sperm. ICSI improved embryo rates and euploidy in male carriers, showing significant differences in chromosome contents between sperm and embryos from male carriers.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Agriculture, Dairy & Animal Science
Estel Vinolas-Verges, Jordi Ribas-Maynou, Isabel Barranco, Camila Peres Rubio, Sergi Bonet, Jordi Roca, Marc Yeste
Summary: Although there were no differences observed in sperm DNA integrity between ejaculate fractions, the SRF-P1 and SRF-P2 exhibited greater chromatin condensation compared to the PSRF. This difference could be related to the oxidative stress index (OSi) of each fraction.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Review
Andrology
Manish Kuchakulla, Manish Narasimman, Kajal Khodamoradi, Zahra Khosravizadeh, Ranjith Ramasamy
Summary: Spermatogenesis is a complex process involving multiple regulatory elements and steps, essential for maintaining and promoting male fertility. Reduced sperm DNA integrity can negatively impact fertility, with sources of DNA damage including replication errors and fragmentation due to various causes like apoptosis and oxidative stress. Research also covers DNA repair mechanisms in spermatozoon.
Article
Obstetrics & Gynecology
Jiangman Gao, Renpei Yuan, Siwei Yang, Yuanyuan Wang, Ying Huang, Liying Yan, Hui Jiang, Jie Qiao
Summary: The study analyzed the correlations between male age, traditional semen parameters, sperm DNA fragmentation index (DFI), and high DNA stainability (HDS) in a large sample size. The results showed that age negatively impacted semen volume, total sperm count, motility, and HDS, while positively affecting sperm concentration and DFI. The study concluded that aging affects sperm quantity and quality, and there are significant correlations between routine sperm parameters and sperm DNA/chromatin integrity.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Cell Biology
Rebecca E. O'Connor, Lucas G. Kiazim, Claudia C. Rathje, Rebecca L. Jennings, Darren K. Griffin
Summary: Pigs are the world's leading source of meat protein, but hypoprolific boars may incur significant economic and environmental losses. Reciprocal translocations are a leading cause of porcine hypoprolificacy, which may be significantly under-reported using traditional karyotyping. A multiple hybridisation fluorescence in situ hybridisation strategy can detect translocations, highlighting the importance of pre-emptive screening before boars enter breeding programs.
Article
Genetics & Heredity
Miluse Vozdova, Svatava Kubickova, Natalia Martinkova, David Javier Galindo, Agda Maria Bernegossi, Halina Cernohorska, Dita Kadlcikova, Petra Musilova, Jose Mauricio Duarte, Jiri Rubes
Summary: The taxonomy and phylogenetics of Neotropical deer have been mainly based on morphological criteria, but this study suggests a critical revision based on new molecular and cytogenetic markers. The analysis of satellite DNA revealed evolutionary relationships among the different Neotropical deer species, especially highlighting the high abundance of satellite DNA in Mazama species and its potential role in chromosomal rearrangements and ongoing speciation.
Article
Genetics & Heredity
David Javier Galindo, Gabriela Siqueira Martins, Miluse Vozdova, Halina Cernohorska, Svatava Kubickova, Agda Maria Bernegossi, Dita Kadlcikova, Jiri Rubes, Jose Mauricio Barbanti Duarte
Summary: The study evaluated the impact of chromosomal polymorphisms as reproductive barriers within the genus Mazama, showing that inter-specific hybrids between Mazama gouazoubira and Mazama nemorivaga exhibited different degrees of fertility reduction, while intra-specific hybrids of Mazama americana with different cytotypes showed varying pairing rates for Robertsonian translocations and tandem fusions.
Article
Multidisciplinary Sciences
Terence J. Robinson, Halina Cernohorska, Svatava Kubickova, Miluse Vozdova, Petra Musilova, Aurora Ruiz-Herrera
Summary: The study focuses on the role of chromosome structural changes in post-zygotic isolation in three antelope species of the genus Raphicerus. While the species are largely conserved in euchromatic regions, their X chromosomes exhibit distinct patterns of heterochromatic amplification and repeat localization. A new hypothesis is proposed suggesting that expansions of heterochromatic blocks in the homogametic sex may contribute to post-zygotic isolation, specifically female hybrid incompatibility.
SCIENTIFIC REPORTS
(2021)
Article
Public, Environmental & Occupational Health
Jiri Rubes, Jaroslav Sipek, Vera Kopecka, Petra Musilova, Miluse Vozdova
Summary: The study investigated sperm quality in city policemen in Ostrava during high and low levels of air pollution. It found that exposure to high air pollution increased sperm chromatin damage and immature sperm, correlating with decreased sperm vitality, normal morphology, and acrosome-intact sperm.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2021)
Article
Reproductive Biology
D. J. Galindo, M. Vozdova, S. Kubickova, H. Cernohorska, A. M. Bernegossi, D. Kadlcikova, J. Rubes, J. M. B. Duarte
Summary: The study used FISH with bovine WCP and BAC probes to analyze chromosome rearrangements and gametic segregation in brown brocket deer, finding a slight to moderate increase in the rates of unbalanced meiotic segregation products in individuals heterozygous for RT and RT/PAIs, but without statistical significance.
Article
Agriculture, Dairy & Animal Science
Miluse Vozdova, Svatava Kubickova, Halina Cernohorska, Jan Frohlich, Jiri Rubes
Summary: This study investigated the chromosome relationships between red deer and cattle, identified errors in the red deer genome assembly, specified cervid evolutionary chromosome breakpoints, and confirmed chromosome fusion in various deer species. The results support necessary improvements in the red deer genome assembly and genetic studies in the Cervidae family.
Review
Cell Biology
Andrigo Barboza de Nardi, Rodrigo dos Santos Horta, Carlos Eduardo Fonseca-Alves, Felipe Noleto de Paiva, Lais Calazans Menescal Linhares, Bruna Fernanda Firmo, Felipe Augusto Ruiz Sueiro, Krishna Duro de Oliveira, Silvia Vanessa Lourenco, Ricardo De Francisco Strefezzi, Carlos Henrique Maciel Brunner, Marcelo Monte Mor Rangel, Paulo Cesar Jark, Jorge Luiz Costa Castro, Rodrigo Ubukata, Karen Batschinski, Renata Afonso Sobral, Natalia Oyafuso da Cruz, Adriana Tomoko Nishiya, Simone Crestoni Fernandes, Simone Carvalho dos Santos Cunha, Daniel Guimaraes Gerardi, Guilherme Sellera Godoy Challoub, Luiz Roberto Biondi, Renee Laufer-Amorim, Paulo Ricardo de Oliveira Paes, Gleidice Eunice Lavalle, Rafael Ricardo Huppes, Fabrizio Grandi, Carmen Helena de Carvalho Vasconcellos, Denner Santos dos Anjos, Angela Cristina Malheiros Luzo, Julia Maria Matera, Miluse Vozdova, Maria Lucia Zaidan Dagli
Summary: Mast cell tumors in dogs are a common and important type of tumor in veterinary oncology. This article presents the report and discussion on the diagnosis, prognosis, and treatment of cutaneous and subcutaneous mast cell tumors in dogs from the 2nd Consensus meeting organized by the Brazilian Association of Veterinary Oncology in August 2021.
Article
Environmental Sciences
Miluse Vozdova, Svatava Kubickova, Vera Kopecka, Jaroslav Sipek, Jiri Rubes
Summary: This study tested the impact of seasonal changes in air pollution on semen quality and sperm DNA methylation in men living in the industrial agglomeration of Ostrava, Czech Republic. The results showed that seasonal changes in air pollution levels did not have a substantial effect on sperm DNA methylation or semen quality.
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2022)
Article
Multidisciplinary Sciences
Miluse Vozdova, Svatava Kubickova, Vera Kopecka, Jaroslav Sipek, Jiri Rubes
Summary: The effects of air pollution on men's reproductive health were investigated in this study. The results showed that sperm mitochondrial DNA copy number (mtDNAcn) was closely associated with male fertility, while mtDNA deletion (mtDNAdel) rates might be a more sensitive biomarker of air pollution exposure in urban industrial environments.
SCIENTIFIC REPORTS
(2022)
Article
Environmental Sciences
Dita Kadlcikova, Petra Musilova, Hana Hradska, Miluse Vozdova, Marketa Petrovova, Marek Svoboda, Jiri Rubes
Summary: The study investigated occupationally induced chromosomal damage in hospital personnel exposed to antineoplastic drugs and/or low doses of ionizing radiation. Both cytogenetic methods showed an increase in chromatid and chromosomal aberrations, as well as unstable aberrations, indicating recent exposure to genotoxic agents.
ARCHIVES OF ENVIRONMENTAL & OCCUPATIONAL HEALTH
(2023)
Article
Zoology
Agda Maria Bernegossi, Carolina Heloisa de Souza Borges, Eluzai Dinai Pinto Sandoval, Jose Luis Cartes, Halina Cernohorska, Svatava Kubickova, Miluse Vozdova, Renato Caparroz, Susana Gonzalez, Jose Mauricio Barbanti Duarte
Summary: This study reviewed and clarified the taxonomy of the gray brocket deer through proposal of an updated nomenclature and characterization of specimens collected near the original type locality. The results supported the polyphyly of the genus Mazama and validated the genus Subulo for the gray brocket deer.
JOURNAL OF MAMMALOGY
(2023)
Article
Biotechnology & Applied Microbiology
Miluse Vozdova, Svatava Kubickova, Jiri Rubes
Summary: Sperm mtDNA status can serve as a molecular marker of oxidative stress and environmental exposure, and high levels of air pollution may be associated with increased mitochondrial DNA deletion rates in sperm.
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2022)
Article
Agriculture, Dairy & Animal Science
Halina Cernohorska, Svatava Kubickova, Petra Musilova, Miluse Vozdova, Roman Vodicka, Jiri Rubes
Summary: This study analyzed a supernumerary marker chromosome in Asian elephants using molecular cytogenetic techniques and proposed its possible origin mechanism. It also explored the relationship between the distribution of nucleolar organizer regions and heterochromatin in elephants.
Article
Cell Biology
Agda Maria Bernegossi, Miluse Vozdova, Halina Cernohorska, Svatava Kubickova, David Javier Galindo, Dita Kadlcikova, Jiri Rubes, Jose Mauricio Barbanti Duarte
Summary: Cervids have the most diverse karyotypes among mammals. In this study, we focused on the gray brocket deer and used molecular cytogenetic markers derived from the cattle genome to map its karyotype. Our results revealed interchromosomal rearrangements and intrachromosomal rearrangements that have separated the cattle and gray brocket deer karyotypes. These findings contribute to the understanding of deer karyotype diversity and taxonomy, and will be valuable for future studies on karyotypic evolution in cervids.
CYTOGENETIC AND GENOME RESEARCH
(2022)
Article
Public, Environmental & Occupational Health
Jiri Rubes, Jaroslav Sipek, Vera Kopecka, Petra Musilova, Miluse Vozdova, Petra Prinosilova, Jan Topinka, Anna Pastorkova, Vlasta Svecova, Radim J. Sram
Summary: This study found a significant positive correlation between paternal age and sperm DNA fragmentation, as well as negative correlations with sperm vitality, acrosome-intact sperm, and normal sperm heads. The average %DFI values varied across different age groups.
HEALTH SCIENCE REPORTS
(2021)