Article
Genetics & Heredity
Miluse Vozdova, Svatava Kubickova, Natalia Martinkova, David Javier Galindo, Agda Maria Bernegossi, Halina Cernohorska, Dita Kadlcikova, Petra Musilova, Jose Mauricio Duarte, Jiri Rubes
Summary: The taxonomy and phylogenetics of Neotropical deer have been mainly based on morphological criteria, but this study suggests a critical revision based on new molecular and cytogenetic markers. The analysis of satellite DNA revealed evolutionary relationships among the different Neotropical deer species, especially highlighting the high abundance of satellite DNA in Mazama species and its potential role in chromosomal rearrangements and ongoing speciation.
Article
Reproductive Biology
D. J. Galindo, M. Vozdova, S. Kubickova, H. Cernohorska, A. M. Bernegossi, D. Kadlcikova, J. Rubes, J. M. B. Duarte
Summary: The study used FISH with bovine WCP and BAC probes to analyze chromosome rearrangements and gametic segregation in brown brocket deer, finding a slight to moderate increase in the rates of unbalanced meiotic segregation products in individuals heterozygous for RT and RT/PAIs, but without statistical significance.
Editorial Material
Genetics & Heredity
Changuk Chung, Xiaoxu Yang, Joseph G. Gleeson
Summary: Brain somatic mosaicism, which is linked to several neurological disorders, may be caused by pre-zygotic aneuploidy followed by post-zygotic partial reversion. This study suggests a recurrent form of epilepsy related to brain mosaicism.
Review
Fisheries
Paula Graziela Lassen, Maria Eugenia Andrighetto Canozzi, Bruna Bitencourt da Costa, Julio Otavio Jardim Barcellos, Danilo Pedro Streit
Summary: The study examined the effects of cryopreservation on sperm motility in South American freshwater fish species. Results showed that the use of cryoprotectants such as alcohols and amides can improve the motility rates of cryopreserved semen.
REVIEWS IN FISHERIES SCIENCE & AQUACULTURE
(2022)
Article
Agriculture, Dairy & Animal Science
Miluse Vozdova, Svatava Kubickova, Halina Cernohorska, Jan Frohlich, Jiri Rubes
Summary: This study investigated the chromosome relationships between red deer and cattle, identified errors in the red deer genome assembly, specified cervid evolutionary chromosome breakpoints, and confirmed chromosome fusion in various deer species. The results support necessary improvements in the red deer genome assembly and genetic studies in the Cervidae family.
Article
Agronomy
Kai Wang, Hui Zhang, Haris Khurshid, Ayman Esh, Caiwen Wu, Qinnan Wang, Nathalie Piperidis
Summary: This paper reviews the application and progress of cytogenetic techniques in the genus Saccharum, and discusses the future applications of cytogenetics in genomic studies and breeding of sugarcane and other plants with complex genomes.
Article
Fisheries
Paula Graziela Lassen, Maria Eugenia Andrighetto Canozzi, Bruna Bitencourt da Costa, Julio Otavio Jardim Barcellos, Danilo Pedro Streit
Summary: The study found that after cryopreservation of sperm from South American freshwater fish species, there was a significant increase in primary morphological abnormalities, while the percentage of normal cells did not change significantly. There was no significant change in the percentage of secondary abnormalities between fresh semen and cryopreserved semen.
AQUACULTURE RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Tao Zhang, Guanqing Liu, Hainan Zhao, Guilherme T. Braz, Jiming Jiang
Summary: Oligonucleotide (oligo)-fluorescence in situ hybridization (FISH) has become a new generation of FISH technique in plant molecular cytogenetics research. Chorus2 software effectively selects single-copy oligos, removes repetitive elements, and allows the development of FISH probes from plant species without an assembled genome, broadening its application in molecular cytogenetics research.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Multidisciplinary Sciences
Juliano Morais, Renato A. Morais, Sterling B. Tebbett, Morgan S. Pratchett, David R. Bellwood
Summary: The study found that Acropora and massive Porites coral colonies exhibited significant differences in survival and growth after bleaching events, with Acropora showing a marked capacity for apparent recovery while massive Porites displayed stable survival. This contrast highlights different dynamics of boom-and-bust vs. protracted declines in the two major coral groups.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Josie Schwartz Caldas, Adriele Lima da Silva, Leandro Melo de Sousa, Erilda Barbosa de Sousa, Iann Leonardo Pinheiro Monteiro, Fabio Jose Torres de Barros, Leandro Godoy
Summary: This study investigated the possibility of obtaining sperm from the endangered Hypancistrus zebra without hormonal induction, and found that hormonal treatment resulted in males releasing a higher volume of semen with higher quality. The use of exogenous hormones is crucial for controlling the reproductive cycle of fish species in captivity, especially for those facing extinction.
Article
Oncology
Daniel Mettman, Azhar Saeed, Janna Shold, Raquele Laury, Andrew Ly, Irfan Khan, Shivani Golem, Mojtaba Olyaee, Maura O'Neil
Summary: The study found that UroVysion was the most sensitive method, with all methods having high specificity. Cytology, forceps biopsies, and fine needle aspiration were also proven to have certain sensitivity and specificity for detecting malignancy in pancreatobiliary strictures. The most effective approach to maintaining high sensitivity while preserving specificity was limiting malignant interpretation to cases with 5+ cells showing the same polysomic signal pattern and/or loss of one or both 9p21 signals.
Article
Genetics & Heredity
Zhoujian He, Wei Zhang, Xiaomei Luo, Jie Huan
Summary: This study analyzed the karyotypes of five Fabaceae species using oligonucleotide probes, revealing their chromosomal organization and variation patterns. The results provide important molecular cytogenetics data and contribute to the construction of a molecular barcode for Fabaceae.
Article
Genetics & Heredity
Katherine E. Miller, Adithe C. Rivaldi, Noriyuki Shinagawa, Sahib Sran, Jason B. Navarro, Jesse J. Westfall, Anthony R. Miller, Ryan D. Roberts, Yassmine Akkari, Rachel Supinger, Mark E. Hester, Mohammad Marhabaie, Meethila Gade, Jinfeng Lu, Olga Rodziyevska, Meenakshi B. Bhattacharjee, Gretchen K. Von Allmen, Edward Yang, Hart G. W. Lidov, Chellamani Harini, Manish N. Shah, Jeffrey Leonard, Jonathan Pindrik, Ammar Shaikhouni, James E. Goldman, Christopher R. Pierson, Diana L. Thomas, Daniel R. Boue, Adam P. Ostendorf, Elaine R. Mardis, Annapurna Poduri, Daniel C. Koboldt, Erin L. Heinzen, Tracy A. Bedrosian
Summary: Somatic mosaicism is a known cause of neurological disorders, and this study reveals a novel mechanism for brain mosaicism through post-zygotic rescue of meiotic errors. The researchers found evidence of extra chromosome 1q allele in the brain tissue of patients with focal epilepsy, which was not observed in blood or buccal cells. This copy number gain was highly enriched in astrocytes and showed distinct gene expression signatures and hyaline inclusions.
Article
Cell Biology
Chenfei Wang, Chuan Chen, Xiaoyu Liu, Chong Li, Qiu Wu, Xiaolan Chen, Lingyue Yang, Xiaochen Kou, Yanhong Zhao, Hong Wang, Yawei Gao, Yong Zhang, Shaorong Gao
Summary: This study investigated the dynamic changes in nucleosome occupancy and positioning in pronucleus-stage zygotes using a new method. The researchers found that nucleosomes were established more quickly in the paternal genome and were associated with specific transcription factor binding sites. These findings have important implications for understanding the mechanisms of chromatin remodeling after fertilization and key transcription regulators during development.
Review
Agriculture, Dairy & Animal Science
Luciana Diniz Rola, Marcos Eli Buzanskas, Luciana Magalhaes Melo, Maiana Silva Chaves, Vicente Jose Figueiredo Freitas, Jose Mauricio Barbanti Duarte
Summary: Deer species in the Neotropical region are experiencing population declines and decreased genetic diversity due to rapid habitat loss and human activities. Assisted reproductive technology (ART) shows promise in advancing wildlife conservation efforts and maintaining genetic diversity in deer. Techniques such as in vitro embryo production (IVP) and proteomics are being utilized to better understand and address reproductive challenges facing Neotropical deer.
Article
Environmental Sciences
Dita Kadlcikova, Petra Musilova, Hana Hradska, Miluse Vozdova, Marketa Petrovova, Marek Svoboda, Jiri Rubes
Summary: The study investigated occupationally induced chromosomal damage in hospital personnel exposed to antineoplastic drugs and/or low doses of ionizing radiation. Both cytogenetic methods showed an increase in chromatid and chromosomal aberrations, as well as unstable aberrations, indicating recent exposure to genotoxic agents.
ARCHIVES OF ENVIRONMENTAL & OCCUPATIONAL HEALTH
(2023)
Article
Zoology
Agda Maria Bernegossi, Carolina Heloisa de Souza Borges, Eluzai Dinai Pinto Sandoval, Jose Luis Cartes, Halina Cernohorska, Svatava Kubickova, Miluse Vozdova, Renato Caparroz, Susana Gonzalez, Jose Mauricio Barbanti Duarte
Summary: This study reviewed and clarified the taxonomy of the gray brocket deer through proposal of an updated nomenclature and characterization of specimens collected near the original type locality. The results supported the polyphyly of the genus Mazama and validated the genus Subulo for the gray brocket deer.
JOURNAL OF MAMMALOGY
(2023)
Article
Genetics & Heredity
Martin Knytl, Nicola R. Fornaini, Barbora Bergelov, Vaclav Gvozdik, Halina Cernohorska, Svatava Kubickova, Eric B. Fokam, Ben J. Evans, Vladimir Krylov
Summary: In this study, cytogenetic and molecular FISH techniques were used to investigate the chromosome morphology and rearrangements in an allotetraploid species of the sister subgenus Silurana. The results showed that the subgenomes of this species have distinctive characteristics, with one subgenome being more conserved and resembling a closely related diploid species, while the other subgenome evolves more rapidly and exhibits pronounced changes in chromosome structure.
Article
Veterinary Sciences
Luis O. Baselly-Cueva, Gabriel B. Melo-Neto, David J. Galindo, Heloisa C. Lima, Sheila C. Rahal
Summary: This study reported a case of mandibular symphyseal separation in a male Amazonian brown brocket buck. A surgical repair technique using parallel stainless steel cerclage wires was introduced. After the surgical repair, the deer had normal dental occlusion and no difficulty in prehension of food.
VETERINARY RECORD CASE REPORTS
(2023)
Article
Zoology
Jorge Alfonso Morales-Donoso, Gabrielle Queiroz Vacari, Agda Maria Bernegossi, Eluzai Dinai Pinto Sandoval, Pedro Henrique Faria Peres, David Javier Galindo, Benoit de Thoisy, Miluse Vozdova, Svatava Kubickova, Jose Mauricio Barbanti Duarte
Summary: A study on the taxonomic classification of Mazama nemorivaga in the Amazon region revealed morphological and cytogenetic differences with other species, suggesting the need to reposition it into a different genus. The study proposed an update to the genus name and suggested further research on the existence of other species within the genus.
Article
Agriculture, Dairy & Animal Science
Halina Cernohorska, Svatava Kubickova, Petra Musilova, Miluse Vozdova, Roman Vodicka, Jiri Rubes
Summary: This study analyzed a supernumerary marker chromosome in Asian elephants using molecular cytogenetic techniques and proposed its possible origin mechanism. It also explored the relationship between the distribution of nucleolar organizer regions and heterochromatin in elephants.
Article
Agriculture, Dairy & Animal Science
Bianca Ferrari, David Javier Galindo, Lindsay Unno Gimenes, Jose Mauricio Barbanti Duarte
Summary: This study aimed to evaluate minimally invasive protocols for estrus synchronization in the brown brocket deer. Different treatments were tested, and it was found that DIP, MGA 1x, and MGA 2x were effective in estrus synchronization, while the high dose of P4LA resulted in poor outcomes.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Multidisciplinary Sciences
Gabriella Saloni Duarte, David Javier Galindo, Maria Helena Mazzoni Baldini, Jeferson Ferreira da Fonseca, Jose Mauricio Barbanti Duarte, Maria Emilia Franco Oliveira
Summary: The present study aimed to test the efficiency of transcervical artificial insemination techniques with different methods of cervical immobilization in the brown brocket deer. The results showed that both TCAI-CI and TCAI-CT techniques were successful in achieving artificial insemination, regardless of using OT as a cervical dilation protocol. This method has a high success rate and applicability, and is beneficial for the conservation of deer species.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
Vanessa S. Altino, Darilia C. B. Rezende, Selene S. C. Nogueira, Leticia G. Aldrigui, Mar Roldan, Jose M. B. Duarte, Carole Fureix, Michael Mendl, Sergio L. G. Nogueira-Filho
Summary: Monitoring the concentration of glucocorticoid metabolites (GCMs) in faecal samples is a non-invasive tool for evaluating stress in wild species. This study aimed to validate the use of GCM concentration as an indicator of adrenal activity and identify behavioural indicators of distress. Four male spotted pacas were subjected to an adrenocorticotropic hormone (ACTH) challenge-test, with changes in GCM concentration and behavioural indicators recorded. The results showed a peak in GCM concentration 24 hours after high-dose ACTH injection, and spotted pacas exhibited decreased exploration and feeding, as well as increased time in the inactive but awake state.
Article
Zoology
Eluzai Dinai Pinto Sandoval, Gabrielle Queiroz Vacari, Juan Pablo Julia, Susana Gonzalez, Miluse Vozdova, Halina Cernohorska, Svatava Kubickova, Daniela C. Kalthoff, Jose Mauricio Barbanti Duarte
Summary: This study conducted an integrative assessment using morphological, cytogenetical, and molecular data to clarify the taxonomy of M. simplicicornis argentina. The results showed that the Argentinian gray brocket is actually the same species as the Paraguayan gray brocket, S. gouazoubira. The study used qualitative skull features, cranio-morphometric results, diploid chromosome number, and phylogenetic analysis to support this conclusion.
ZOOLOGICAL STUDIES
(2023)
Article
Zoology
Eluzai Dinai Pinto Sandoval, Agda Maria Bernegossi, Sonia Gallina, Rafael Reyna-Hurtado, Jose Mauricio Barbanti Duarte
Summary: The Yucatan brown brocket deer, initially described as Mazama pandora, was repositioned to the genus Odocoileus based on mitochondrial DNA analysis. There is still a lack of consensus on the taxonomic classification of this species. This study aims to clarify the taxonomy of O. pandora using morphological, cytogenetic, and molecular data, and suggests that a new generic name should be indicated for this taxon.
CANADIAN JOURNAL OF ZOOLOGY
(2023)
