Article
Cell Biology
Zhengqing Hu, Fnu Komal, Aditi Singh, Meng Deng
Summary: This study aimed to generate an SGN-specific damage model using Cre-LoxP transgenic mouse strains, successfully evaluating SGN cell loss through tamoxifen treatment and diphtheria toxin injection in mice. The Nefl (CreER/CreER) mouse strain exhibited inducible SGN-specific Cre activity in the inner ear, serving as a valuable model for regeneration research of the inner ear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jimena Barbeito-Andres, Laura Andrini, Mariana Vallejo-Azar, Sara Seguel, Jay Devine, Benedikt Hallgrimsson, Paula Gonzalez
Summary: This article introduces a protocol for obtaining high-resolution 3D information on mouse neonate brains and skulls using micro-CT, and describes the steps and image analysis methods involved. The study shows that micro-CT with Lugol's solution can be a suitable alternative for imaging the perinatal brains of small animals.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Multidisciplinary Sciences
Charles Petitpre, Louis Faure, Phoebe Uhl, Paula Fontanet, Iva Filova, Gabriela Pavlinkova, Igor Adameyko, Saida Hadjab, Francois Lallemend
Summary: This study used deep, single-cell transcriptomics to investigate the molecular mechanisms that govern the identity and organization of spiral ganglion neurons (SGNs) in mice. The study identified a core set of temporally patterned genes and gene regulatory networks that contribute to the diversification of SGNs through sequential binary decisions. The results also revealed that cell-cell signaling is potentially involved in the differentiation of SGNs.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Christopher B. Buswinka, Richard A. Osgood, Rubina Simikyan, David Rosenberg, Artur Indzhykulian
Summary: Our sense of hearing is mediated by sensory hair cells which are precisely arranged and highly specialized cells subdivided into outer and inner hair cells. Currently, there is a lack of widely applicable tools for fast, unbiased, and comprehensive image analysis of auditory hair cells. In this study, we present HCAT, a highly accurate machine learning-based hair cell analysis toolbox, which automates common image analysis tasks and allows for faster and more comprehensive data analysis.
Article
Cell & Tissue Engineering
Aleta R. Steevens, Matthew W. Griesbach, Yun You, James R. Dutton, Walter C. Low, Peter A. Santi
Summary: This study successfully produced induced pluripotent stem cell-derived inner ear sensory neurons in a mouse model using blastocyst complementation. The approach not only corrected non-sensory deficits associated with Neurog1 heterozygosity, but also highlighted blastocyst complementation as a potential tool for creating chimeric inner ear cell types for transplantation into damaged inner ears to improve hearing.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Multidisciplinary Sciences
Zelun Wang, Sean McCracken, Philip R. Williams
Summary: This study explores the transformation of light signals into electrical signals in retinal diseases, using in vivo microscopy in animal models for understanding neurodegeneration and developing treatments. Through various labeling techniques, high-resolution imaging of cellular cohorts in the mouse retina is achieved, along with detailed strategies for manual image postprocessing.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Audiology & Speech-Language Pathology
Courtney Penn, Karthick Mayilsamy, Xiao Xia Zhu, Mark A. Bauer, Shyam S. Mohapatra, Robert D. Frisina, Subhra Mohapatra
Summary: Traumatic Brain Injury (TBI) is a major cause of death and disability worldwide. Even mild TBI injuries, which make up the majority of cases, can lead to chronic post-concussive neurological symptoms, including auditory dysfunction. The mechanism by which indirect damage causes hearing loss is poorly understood, and current treatment is limited to managing symptoms. This study aims to establish a model of indirect damage to the auditory system caused by repetitive TBIs and investigate the pathology of hearing loss.
Article
Geriatrics & Gerontology
Akitoshi Mitani, Hiroshi Iwai, Muneo Inaba, Dan Van Bui, Toru Miwa, Linh Manh Nguyen, Chu Hong Hanh, Tomofumi Sakagami, Kensuke Suzuki, Yoshiki Kobayashi, Yasutaka Yun, Yuuki Sugita, Akira Kanda
Summary: This study aimed to determine whether immuno-rejuvenation procedures can prevent age-related hearing loss (ARHL) and have clinical applications, as well as to analyze the communication mechanisms between the systemic immune system and the cochlea using a murine model. Lymphocytes from young mice inhibited the progression of ARHL and their cryopreservation and inoculation at the onset of ARHL suggests their clinical application. This treatment not only maintained auditory threshold but also prevented spinal ganglion degeneration, cellular immune aging, and nitric oxide production, which causes age-related tissue damage.
EXPERIMENTAL GERONTOLOGY
(2023)
Article
Endocrinology & Metabolism
Pholpat Durongbhan, Mateus O. Silva, Zihui Li, Niloufar Ansari, R. Y. Nigel Kour, Catherine E. Davey, Kathryn S. Stok
Summary: This study proposes a novel microCT imaging protocol for reproducible and efficient quantitative morphometric analysis (QMA) of in situ mouse tibio-femoral joint. The study includes a diffusion kinetics study for a contrast agent and a joint positioning and image processing workflow. Results show that reliable measurement of cartilage can be achieved after contrast agent diffusion, and the imaging protocol enables reproducible and efficient QMA of joint structures. The workflow for whole joint QMA showed excellent reproducibility.
Article
Chemistry, Multidisciplinary
Menghui Liao, Yangnan Hu, Yuhua Zhang, Kaichen Wang, Qiaojun Fang, Yanru Qi, Yingbo Shen, Hong Cheng, Xiaolong Fu, Mingliang Tang, Shan Sun, Xia Gao, Renjie Chai
Summary: Cochlear implantation is the most effective treatment method for patients with profound and total hearing loss. This study presents an electrical stimulation system combining a cochlear implant and a conductive Ti3C2Tx MXene-matrigel hydrogel, which promotes the growth and signal transmission of spiral ganglion neurons, potentially improving the postoperative listening effect of cochlear implantation.
Article
Multidisciplinary Sciences
R. Benjamin Sulser, Bruce D. Patterson, Daniel J. Urban, April I. Neander, Zhe-Xi Luo
Summary: Phylogenomics of bats suggests that their echolocation either evolved separately in the bat suborders Yinpterochiroptera and Yangochiroptera, or had a single origin in bat ancestors and was later lost in some yinpterochiropterans. The observation of highly derived structures of the spiral ganglion in yangochiropteran bats suggests a neuroanatomical evolutionary driver for their diverse echolocating strategies. These features are highly variable across major clades and may be associated with the explosive diversification of yangochiropterans.
Article
Endocrinology & Metabolism
Shyan-Yuan Kao, Sachiyo Katsumi, Dongjun Han, Argyro J. Bizaki-Vallaskangas, Sasa Vasilijic, Lukas D. Landegger, Arthur G. Kristiansen, Michael J. McKenna, Konstantina M. Stankovic
Summary: The study demonstrates that RANK and RANKL inhibit neurite outgrowth in cochlear neurons in mice, in addition to regulating otic capsule bone remodeling.
Article
Ophthalmology
Vidisha Goyal, A. Thomas Read, Matthew D. Ritch, Bailey G. Hannon, Gabriela Sanchez Rodriguez, Dillon M. Brown, Andrew J. Feola, Adam Hedberg-Buenz, Grant A. Cull, Juan Reynaud, Mona K. Garvin, Michael G. Anderson, Claude F. Burgoyne, C. Ross Ethier
Summary: AxoNet 2.0, an automated deep learning tool, was developed to accurately quantify retinal ganglion cell (RGC) axonal loss and morphologic change. The tool showed excellent performance and promising generalization on different animal models. This study is of great importance for investigating glaucomatous optic neuropathy in animal models.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2023)
Article
Audiology & Speech-Language Pathology
Mingyu Xia, Mingxuan Wu, Liping Zhao, Jiaoyao Ma, Wenyan Li, Huawei Li
Summary: Loss of hair cells is a major factor in sensorineural hearing loss, and regeneration of cochlear hair cells is seen as a way to restore hearing. A new transgenic mouse model was created to selectively ablate inner hair cells (IHCs) in a dose-dependent manner, leading to the observation of regeneration from Lgr5+ progenitors. However, most regenerated hair cells did not survive to adulthood, and loss of spiral ganglion neurons resulted in profound hearing loss after the ablation of IHCs. This model shows promise for studying mechanisms of IHC loss and regeneration.
Article
Neurosciences
Xiaoyu Wang, Qiwei Fan, Xiaoyan Yu, Yuan Wang
Summary: In this study, the researchers investigated the distribution and expression of FMRP in the inner ear of mice, rats, gerbils, and chickens. They found that FMRP was present in hair cells, supporting cells, and auditory ganglion neurons. The distribution of FMRP in hair cells showed age-dependent translocation, while FMRP in auditory ganglion neurons exhibited high intensity during both development and maturation. These findings suggest the involvement of FMRP in inner ear development and its potential contribution to auditory dysfunction in Fragile X syndrome.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Neurosciences
Peter A. Santi, Robair Aldaya, Alec Brown, Shane Johnson, Tyler Stromback, Sebahattin Cureoglu, Helge Rask-Andersen
JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
(2016)
Article
Neurosciences
Rachel M. Anderson, Ryan M. Glanz, Shane B. Johnson, Mary M. Miller, Sara A. Romig-Martin, Jason J. Radley
JOURNAL OF COMPARATIVE NEUROLOGY
(2016)
Article
Neurosciences
Shane B. Johnson, Eric B. Emmons, Rachel M. Anderson, Ryan M. Glanz, Sara A. Romig-Martin, Nandakumar S. Narayanan, Ryan T. LaLumiere, Jason J. Radley
JOURNAL OF NEUROSCIENCE
(2016)
Article
Endocrinology & Metabolism
Jason J. Radley, Shane B. Johnson
PSYCHONEUROENDOCRINOLOGY
(2018)
Article
Neurosciences
Keiko Hirose, Jared J. Hartsock, Shane Johnson, Peter Santi, Alec N. Salt
JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
(2014)
Article
Otorhinolaryngology
Heather M. Schmitz, Shane B. Johnson, Peter A. Santi
OTOLARYNGOLOGY-HEAD AND NECK SURGERY
(2014)
Article
Neurosciences
Shane B. Johnson, Eric B. Emmons, Ryan T. Lingg, Rachel M. Anderson, Sara A. Romig-Martin, Ryan T. LaLumiere, Nandakumar S. Narayanan, Victor Viau, Jason J. Radley
JOURNAL OF NEUROSCIENCE
(2019)
Article
Neurosciences
Rachel M. Anderson, Shane B. Johnson, Ryan T. Lingg, Dalton C. Hinz, Sara A. Romig-Martin, Jason J. Radley
Article
Multidisciplinary Sciences
Ryan T. Lingg, Shane B. Johnson, Eric B. Emmons, Rachel M. Anderson, Sara A. Romig-Martin, Nandakumar S. Narayanan, James L. McGaugh, Ryan T. LaLumiere, Jason J. Radley
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Editorial Material
Neurosciences
Shane Johnson, Conor Liston
Summary: Research shows that MeCP2 signaling in the hippocampus is crucial for sustaining antidepressant effects.
NATURE NEUROSCIENCE
(2021)
Review
Neurosciences
Puja K. Parekh, Shane B. Johnson, Conor Liston
Summary: Depression is characterized by mood state transitions with poorly understood neurobiological mechanisms. Antidepressants reverse the effects of stress and depression on synapse function, suggesting that synaptic remodeling plays a key role in depression pathophysiology and antidepressant function. Antidepressants initially act by facilitating plasticity and reconfiguring the brain state, with synaptogenesis sustaining these changes over time.
ANNUAL REVIEW OF NEUROSCIENCE
(2022)
Article
Audiology & Speech-Language Pathology
Peter A. Santi, Shane Johnson, Julian Wuester, Matthew Griesbach, Alexander Claussen, Jonathon Kirk
Summary: Postmortem examination of the cochlea with a cochlear implant presents challenges due to the presence of wires and metal components that make sectioning difficult. Plastic embedded implanted cochleas have been successfully sectioned using specialized methods. An alternative non-destructive method is optical sectioning of a chemically cleared cochlea, but the presence of metal components results in artifacts in the 2D optical sections. Removal of the implant prior to imaging yields the best image quality.
Article
Multidisciplinary Sciences
Shane B. Johnson, Ryan T. Lingg, Timothy D. Skog, Dalton C. Hinz, Sara A. Romig-Martin, Victor Viau, Nandakumar S. Narayanan, Jason J. Radley
Summary: This study explores the link between behavioral and biological features of stress responses in the brain. It identifies the involvement of the medial prefrontal cortex (mPFC) and midbrain dorsolateral periaqueductal gray (PAG) in active stress coping behaviors, while a different circuit involving the rostral mPFC and ventrolateral PAG limits passive behaviors. Furthermore, the activation of the caudal mPFC-dorsolateral PAG projection can attenuate the increase in stress hormone output and immobility associated with a passive coping response. These findings provide insights into how the brain coordinates different responses to stress and contributes to stress resilience.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Peter A. Santi, Shane B. Johnson
Summary: Deafness is a common sensory impairment, particularly in the elderly population. However, studying the relationship between the structure and function of the inner ear has been challenging. To overcome this, researchers have developed a light-sheet microscope that can image and digitize the entire cochlea, enabling a better understanding of its structure and function. Using this technique, researchers were able to quantify the loss of hair cells and spiral ganglion neurons, as well as observe local reductions in spiral ganglion neurons within the cochlea.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Clinical Neurology
Shane B. Johnson, Sebahattin Cureoglu, Jennifer T. O'Malley, Peter A. Santi
OTOLOGY & NEUROTOLOGY
(2014)
