Effects of Artificial Light Spectra on Anthocyanin Accumulation in Blueberry Callus Cultures

作者

Gamil Rayan Abou El-Dis¹ , Khusnetdinova Landysh Zavdetovna² , Akulov Anton Nikolaevich³ , Walla Mohamed Abdelmaksood Abdelazeez⁴ , Timofeeva Olga Arnoldovna²

1. Desert Research Center, Cairo, Egypt
2. Kazan Federal University, Kazan, Russia
3. Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS
4. Faculty of Science, Al-Azhar University, Cairo

会议/活动

International Annual Conference on Basic and Applied Science (IACBAS 2022) Towards "Advanced Science & Sustainable Development", March 2022 (Cairo, Egypt)

海报摘要

Blueberry is considered a health-promoting food with a high content of anthocyanins. The extraction of anthocyanins from callus has been used to solve some problems of anthocyanins production, being more effective for commercial application and high production of anthocyanins. One of the most important aims of our study is to enhance anthocyanin synthesis in callus cultures. The results showed that callus induction from the highbush blueberry V. corymbosum L. cv. Sunt Blue Giant depends on the type of explant and culture medium. Maximum callus induction was established on Woody Plant medium (WPM) containing 2.0 mg/L ɑ–naphthalene acetic acid (NAA) with 1.0 mg/L 6-benzylaminopurine/benzyl adenine (BAP) from all used explants and the maximum fresh weight (for leaf, 1.03; root, 1.0; and stem, 0.8 g/jar) was recorded. After four subcultures on the same medium composition, we studied the effect of different light types on the accumulation of anthocyanins in callus culture.

关键词

Blueberry, Blue light, In vitro, Subculture, Red light

研究领域

Agriculture, Biochemistry, Biological Sciences

参考文献

1. Miao L, Zhang Y, Yang X, Xiao J, Zhang H, Zhang Z, Wang Y, Jiang G (2016) Colored light-quality selective plastic films affect anthocyanin content, enzyme activities, and the expression of flavonoid genes in strawberry (Fragaria× ananassa) fruit. Food chemistry 207:93–100. https://doi.org/10.1016/j.foodchem.2016.02.077
2. Wu X, Beecher GR, Holden JM, Haytowitz DB, Gebhardt SE, Prior RL (2006) Concentrations of anthocyanins in common foods in the United States and estimation of normal consumption. Journal of agricultural and food chemistry 54(11):4069–4075. https://doi.org/10.1021/jf060300l
3. Li C, Feng J, Huang W-Y, An X-T (2013) Composition of polyphenols and antioxidant activity of rabbiteye blueberry (Vaccinium ashei) in Nanjing. Journal of Agricultural and Food Chemistry 61(3):523–531. https://doi.org/10.1021/jf3046158
4. Rowland LJ, Hammerschlag FA. 8.1 Vaccinium spp. Blueberry (2005). In: Litz R (ed) Biotechnology of Fruit and Nut Crops. Biotechnology in Agriculture CABI Publishing, Wallingford, UK Series no 29 :222–246. (Accessed 29 October 2020).
5. Lila MA (2004) Anthocyanins and human health: an in vitro investigative approach. BioMed Research International (5):306–313. https://doi.org/10.1155/S111072430440401X
6. Ram M, Prasad K, Kaur C, Singh S, Arora A, Kumar S (2011) Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels. Plant Cell Tissue and Organ Culture (PCTOC) 104(2):171–179. https://doi.org/10.1007/s11240-010-9814-5
7. Debnath SC, Goyali JC (2020) In Vitro Propagation and Variation of Antioxidant Properties in Micropropagated Vaccinium Berry Plants—A Review. Molecules 25(4):788. https://doi.org/10.3390/molecules25040
8. Migas P, Cisowski W, Dembińska-Migas W (2005) Isoprene derivatives from the leaves and callus cultures of Vaccinium corymbosum var. Bluecrop. Acta Pol Pharm 62: 45–51
9. Migas P, Luczkiewicz M, Cisowski W (2006) The influence of auxins on the biosynthesis of isoprene derivatives in callus cultures of Vaccinium corymbosum var. bluecrop. Z Naturforsch C 61:565–570. https://doi.org/10.1515/znc-2006-7-816
10. Simões C, Bizarri CHB, da Silva Cordeiro L, de Castro TC, Coutada LCM, da Silva AJR, Albarello N, Mansur E (2009) Anthocyanin production in callus cultures of Cleome rosea: modulation by culture conditions and characterization of pigments by means of HPLC-DAD/ESIMS. Plant Physiology and Biochemistry 47(10):895–903. https://doi.org/10.1016/j.plaphy.2009.06.005

基金

暂无数据

补充材料

暂无数据

附加信息

利益冲突

No competing interests were disclosed.

数据可用性声明

The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.

知识共享许可协议

Copyright © 2022 Abou El-Dis et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.