Article
Hematology
Jiayin Tian, Murray J. Adams, Jasmine Wee Ting Tay, Ian James, Suzanne Powell, Quintin W. Hughes, Grace Gilmore, Ross I. Baker, Jim Yu-Hsiang Tiao
Summary: The study found that plasma samples from pregnant women and women taking contraceptive pills displayed hypercoagulability compared to controls. Under high physiological levels of E (2), miR-365a-3p was downregulated and tissue factor protein was upregulated. This suggests that miR-365a-3p regulates tissue factor and high E (2) levels induce a hypercoagulable state through a specific miR network targeting coagulation factors.
THROMBOSIS AND HAEMOSTASIS
(2021)
Review
Hematology
Jun Wan, Joke Konings, Bas de Laat, Tilman M. Hackeng, Mark Roest
Summary: The study discusses plasma thrombin generation (TG) as a tool to assess blood coagulability, highlighting the importance of also considering the role of blood cells in coagulation. Platelets play a significant role in accelerating thrombin generation through various mechanisms, while erythrocytes and other blood cells also contribute to the coagulation process. Further research is needed to validate blood cell-dependent TG assays for clinical use.
THROMBOSIS AND HAEMOSTASIS
(2021)
Review
Critical Care Medicine
Jessica C. Cardenas
Summary: Thrombin plays a central role in coagulation, essential for normal hemostasis. The generation of thrombin in severely injured patients is closely related to the risks of bleeding and thrombotic complications, and understanding the determinants of its production is clinically important.
Article
Pharmacology & Pharmacy
Giovanni Cimmino, Stefano Conte, Andrea Morello, Grazia Pellegrino, Laura Marra, Gaetano Cali, Paolo Golino, Plinio Cirillo
Summary: The study found that colchicine significantly reduced TF gene and protein expression, as well as its procoagulant activity in oxLDL-treated HUVEC. These effects appear to be mainly related to colchicine's action on microtubules, modulating TF trafficking in the cytoplasm, NF-kappa B/I kappa B pathway, and LOX-1 expression.
VASCULAR PHARMACOLOGY
(2021)
Article
Hematology
Danielle White, Stephen MacDonald, Tara Edwards, Chris Bridgeman, Megan Hayman, Megan Sharp, Sally Cox-Morton, Emily Duff, Swati Mahajan, Chloe Moore, Melissa Kirk, Richard Williams, Martin Besser, Will Thomas
Summary: This study aimed to explore thrombin generation in COVID-19 patients using a novel analyzer. Critical patients showed increased levels of fibrinogen, CRP, interleukin-6, and D-dimer compared to noncritical patients. Although critical patients had right-shifted thrombin generation, there were no significant differences in key thrombin generation parameters. Increased levels of tPA, TFPI, and VEGF in critical patients may indicate endothelial dysfunction or heparin-induced effects.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2021)
Article
Hematology
Thomas Sinegre, Cedric Duron, Thomas Lecompte, Geraldine Lamblin, Laurie Talon, Leon Muti, Sylvie Massoulier, Bruno Pereira, Aurelien Lebreton, Armand Abergel
Summary: The aim of this study was to compare the intraindividual variability of thrombin generation over time between patients with cirrhosis and healthy controls. The results showed that patients with cirrhosis had higher endogenous thrombin potential when using the thrombin generation assay, and this increased with the severity of cirrhosis. The thrombin generation parameters were highly stable over time in healthy controls, moderately stable in Child-Turcotte-Pugh (CTP)-A patients, and poorly stable in CTP-B/C patients (severe cirrhosis), with one-third of patients transitioning to normal or hypercoagulable states during the 3-month follow-up.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Yideng Liang, Ivan Tarandovskiy, Stepan S. Surov, Mikhail V. Ovanesov
Summary: Preclinical evaluation of drugs in animals is crucial in selecting useful clinical laboratory markers for human trials. The sensitivity of animal plasmas to triggers of thrombin generation (TG), specifically human Tissue Factor (TF) and Activated Factor XI (FXIa), was studied. The results showed that animal plasmas have widely variable sensitivities to TF and FXIa, making it challenging to extrapolate animal model results to humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Hematology
Nikolaus B. Binder, Francois Depasse, Julia Mueller, Thomas Wissel, Stephan Schwers, Matthias Germer, Bjorn Hermes, Peter L. Turecek
Summary: TGA is a global assay that allows for the dynamic and continuous recording of both thrombin generation and inactivation effects. It is widely used in clinical medicine for the diagnosis, prognosis, prophylaxis, and treatment of inherited and acquired bleeding and thrombotic disorders.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Ruiqi Yin, Vishal Patel, Rida A. Malik, James C. Fredenburgh, Jeffrey I. Weitz
Summary: Polyphosphate (polyP) serves as a template to promote feedback activation of factor XI by thrombin. This study determines the contribution of thrombin exosites 1 and 2 to polyP-induced potentiation of FXI activation. The results show that polyP accelerates FXI activation by thrombin and exosite 2 ligands attenuate this activation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Hari Hara Sudhan Lakshmanan, Aldrich Estonilo, Stephanie E. Reitsma, Alexander R. Melrose, Jayaram Subramanian, Tony J. Zheng, Jeevan Maddala, Erik Tucker, David Gailani, Owen J. T. McCarty, Patrick L. Jurney, Cristina Puy
Summary: This study evaluated the contribution of feedback activation of coagulation factor XI (FXI) in TF-mediated thrombin generation using a comprehensive systems-based analysis. The results showed that the feedback activation of FXI contributes to the propagation of thrombin generation based on the initial concentrations of TF or activated coagulation factor X (FXa). Additionally, it was predicted and experimentally validated that the inhibitory function of tissue factor pathway inhibitor (TFPI) can abolish the positive feedback of FXI activation by thrombin.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Pharmacology & Pharmacy
Bozhao Li, Jingyan Wei, Chunzhi Di, Zefang Lu, Feilong Qi, Yinlong Zhang, Wei Sun Leong, Lele Li, Guangjun Nie, Suping Li
Summary: The tumor-specific tTF delivery system developed in this study, by coupling tTF with AS1411, selectively targets tumor vascular endothelial cells, inducing tumor necrosis without significant side effects.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Hematology
Claudia Khayat, Rita Marchi, Stephane Durual, Thomas Lecompte, Marguerite Neerman-Arbez, Alessandro Casini
Summary: This study investigated the impact of fibrinogen concentrate (FC) infusion on thrombin generation parameters and fibrin clot structure in patients with inherited afibrinogenemia. The results showed that FC infusion increased thrombin potential and peaks, but lag times and thrombin peaks were still lower than healthy controls. Fibrin clot structure formed after FC infusion was similar to patients with hypofibrinogenemia. These findings suggest that FC may not induce hypercoagulability in patients with afibrinogenemia.
THROMBOSIS AND HAEMOSTASIS
(2022)
Review
Cardiac & Cardiovascular Systems
Lars L. F. G. Valke, Sanna Rijpma, Danielle Meijer, Saskia E. M. Schols, Waander L. van Heerde
Summary: The treatment of bleeding and thrombotic disorders can be personalized through the use of global assays such as thrombin generation assays (TGAs). These assays can help identify patients with more severe bleeding phenotypes and guide non-replacement therapy and bypassing therapy. They also have a potential role in monitoring antithrombotic therapy.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Hematology
Jun Wan, Nadira Vadaq, Joke Konings, Martin Jaeger, Vinod Kumar, Bas de Laat, Leo Joosten, Mihai G. Netea, Andre J. van der Ven, Philip G. de Groot, Quirijn de Mast, Mark Roest
Summary: The KLKB1 gene was found to be associated with the protein C pathway function and thrombin generation. Kallikrein may play a role in regulating the anticoagulant protein C pathway during thrombin generation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Endocrinology & Metabolism
Rita Indirli, Emanuele Ferrante, Erica Scalambrino, Eriselda Profka, Marigrazia Clerici, Tommaso Lettera, Andreea Liliana Serban, Walter Vena, Alessandro Pizzocaro, Marco Bonomi, Biagio Cangiano, Giulia Carosi, Gherardo Mazziotti, Luca Persani, Andrea Lania, Maura Arosio, Flora Peyvandi, Giovanna Mantovani, Armando Tripodi
Summary: Klinefelter syndrome patients exhibit a procoagulant imbalance with increased thrombin generation in both platelet-poor plasma and platelet-rich plasma, potentially due to elevated FVIII levels. Higher levels of FVIII, fibrinogen, and FVIII/protein C ratio were observed in KS patients compared to controls.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Cardiac & Cardiovascular Systems
Ruben R. De With, Vicente Artola Arita, Bao-Oanh Nguyen, Dominik Linz, Hugo Ten Cate, Henri Spronk, Ulrich Schotten, Anton Jan van Zonneveld, Omer Erkuner, M. Agustina Bayon, Anders S. Schmidt, Justin G. L. M. Luermans, Harry J. G. M. Crijns, Isabelle C. Van Gelder, Michiel Rienstra
Summary: In patients with paroxysmal atrial fibrillation, women tend to have higher levels of inflammatory biomarkers, while men tend to have higher levels of biomarkers related to vascular remodelling. This suggests that the pathophysiological mechanisms in men and women with AF may differ.
Article
Engineering, Biomedical
Anne Willers, Justyna Swol, Sander M. J. von Kuijk, Hergen Buscher, Zoe McQuilten, Hugo ten Cate, Peter T. Rycus, Stephen McKellar, Roberto Lorusso, Joseph E. Tonna
Summary: This study aimed to develop and internally validate a prediction model to calculate the risk for bleeding complications in adult patients receiving V-V ECLS support, based on data from the ELSO registry.
Review
Medicine, General & Internal
Sierk Dauwerse, Hugo ten Cate, Henri M. H. Spronk, Magdolna Nagy
Summary: Hemostasis is a finely tuned process with dysregulation potentially leading to bleeding or thrombotic complications. COVID-19 patients often have a hypercoagulable state and require hospitalization, with ROTEM being considered a promising tool to predict outcomes in these patients.
Editorial Material
Cardiac & Cardiovascular Systems
Magdolna Nagy, Hugo ten Cate
CARDIOVASCULAR RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Vicente Artola Arita, Martijn E. Van de Lande, Neda Khalilian Ekrami, Bao-Oanh Nguyen, Joost M. Van Melle, Bastiaan Geelhoed, Ruben R. De With, Vanessa Weberndorfer, Omer Erkuner, Hans Hillege, Dominik Linz, Hugo Ten Cate, Henri M. H. Spronk, Tim Koldenhof, Robert G. Tieleman, Ulrich Schotten, Harry J. G. M. Crijns, Isabelle C. Van Gelder, Michiel Rienstra
Summary: This study aimed to assess whether the 4S-AF scheme predicts AF progression in patients with self-terminating AF. The study found that the 4S-AF scheme does not predict AF progression, but the 3S-AF scheme can predict progression. Therefore, the 3S-AF scheme may be more appropriate for predicting AF progression.
Editorial Material
Cardiac & Cardiovascular Systems
Aaron Iding, Arina ten Cate-Hoek, Hugo ten Cate
EUROPEAN HEART JOURNAL
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Bruna Gigante, Hugo ten Cate
Summary: Clinical trials are underway to test factor XIa inhibitors as potential alternatives to direct oral anticoagulants, with the hope of reducing bleeding events. Findings from phase II trials suggest a lower risk of bleeding, but the efficacy of these inhibitors is still uncertain.
NATURE REVIEWS CARDIOLOGY
(2023)
Article
Hematology
Aaron F. J. Iding, Bram M. M. Kremers, Alejandro Pallares Robles, Hugo ten Cate, Arina J. ten Cate-Hoek
Summary: This study evaluated an RVO-based management strategy and found that RVO was associated with recurrence, PTS, arterial events, and cancer in patients with DVT. D-dimer levels measured 1 month after stopping anticoagulant therapy provided further insight into the management strategy.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Medicine, General & Internal
Loes H. Willems, Lotte M. C. Jacobs, Laszlo A. Groh, Hugo ten Cate, Henri M. H. Spronk, Boden Wilson-Storey, Gerjon Hannink, Sander M. J. van Kuijk, Chahinda Ghossein-Doha, Magdi Nagy, Dick H. J. Thijssen, Andre S. van Petersen, Michiel C. Warle
Summary: COVID-19 affects the cardiovascular system, leading to sustained microvascular dysfunction, systemic inflammation, and coagulation activation. 18 months after infection, macrovascular dysfunction does not increase, but endothelial cell activation, inflammation, and coagulation activation still persist.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Economics
Rachel H. P. Schreurs, Arina J. ten Cate-Hoek, Hugo ten Cate, Manuela A. Joore
Summary: This study assessed the budget impact of compression therapy following deep venous thrombosis in the Netherlands and found that current therapy is suboptimal. Optimization of initial compression therapy, early consultation of an occupational therapist, and tailored duration of elastic compression stocking therapy can lead to considerable cost savings.
PHARMACOECONOMICS-OPEN
(2023)
Article
Hematology
Agnerys Lopez Sacerio, Mara Caridad Tejeda Ramon, Aliuska Morales Helguera, Yunierkis Perez Castillo, Javier Cruz Rodriguez, Jorge Felix Guerra Rodriguez, Anna Falanga
Summary: This study developed a logistic regression model to predict VTE risk in hospitalized HM patients and validated it both internally and externally. The model includes 5 predictive factors and showed good accuracy and discrimination in predicting VTE risk.
ANNALS OF HEMATOLOGY
(2023)
Article
Oncology
Patricia Gomez-Rosas, Cinzia Giaccherini, Laura Russo, Cristina Verzeroli, Sara Gamba, Carmen Julia Tartari, Silvia Bolognini, Chiara Ticozzi, Francesca Schieppati, Luca Barcella, Roberta Sarmiento, Giovanna Masci, Carlo Tondini, Fausto Petrelli, Francesco Giuliani, Andrea D'Alessio, Mauro Minelli, Filippo De Braud, Armando Santoro, Roberto Labianca, Giampietro Gasparini, Marina Marchetti, Anna Falanga, H. Y. P. E. R. C. A. N. Investigators HYPERCAN Investigators
Summary: This study found that VTE is a common complication in lung cancer patients during chemotherapy and is associated with increased mortality. A new prediction model was developed to stratify the risk of VTE and mortality in lung cancer patients, which showed higher accuracy compared to other existing models.
Review
Oncology
R. A. L. Willems, N. Michiels, V. R. Lanting, S. Bouwense, B. L. J. van den Broek, M. Graus, F. A. Klok, B. Groot Koerkamp, B. de Laat, M. Roest, J. W. Wilmink, N. van Es, J. S. D. Mieog, H. ten Cate, J. de Vos-Geelen
Summary: In the past, patients with pancreatic ductal adenocarcinoma often underwent immediate surgery for tumor resection. Now, more and more patients receive chemotherapy (radiotherapy) before surgery. It is known that pancreatic cancer patients have a higher risk of developing thrombosis. However, the incidence of thrombosis in patients with pancreatic cancer during neoadjuvant chemotherapy is not well-studied. Few studies have investigated VTE incidence in this population, and it is unclear if these patients should use perioperative thromboprophylaxis. This narrative review summarizes the current evidence.
Article
Medicine, Research & Experimental
Oluwatoyosi Muse, Rushad Patell, Christian G. Peters, Moua Yang, Emale El-Darzi, Sol Schulman, Anna Falanga, Marina Marchetti, Laura Russo, Jeffrey I. Zwicker, Robert Flaumenhaft
Summary: Thrombosis is a common complication of advanced cancer, and our study reveals that activation of the unfolded protein response (UPR) in cancer cells can lead to increased release of prothrombotic particles, providing a mechanistic link between ER stress and cancer-associated thrombosis.