Article
Biochemistry & Molecular Biology
Hsin-Yu Chung, Bo-An Lin, Yi-Xuan Lin, Chen-Wei Chang, Wen-Shyong Tzou, Tun-Wen Pei, Chin-Hwa Hu
Summary: Research demonstrates that Hif1 alpha acts downstream of Meis1 to mediate gata1 expression in zebrafish embryos, impacting erythropoiesis. Meis1 and Hif1 alpha form a hierarchical regulatory network, activating gata1 transcription through direct interactions, ensuring the generation of primitive erythrocytes.
Article
Chemistry, Medicinal
Bengisu Turgutalp, Merve Uslu, Sinem Helvacioglu, Mohammad Charehsaz, Enise Ece Gurdal, Wolfgang Sippl, Fatih Kocabas, Mine Yarim
Summary: The pivotal role of MEIS1 in cardiac regeneration and HSC regulation was highlighted, and a potent MEIS inhibitor, MEISi-1, inducing HSC expansion was identified. Lead optimization on MEISi-1 led to the synthesis of 45 novel analogues, with 11 compounds identified as potent inhibitors, including 4h, 4f, and 4b exhibiting the best inhibitory profile on MEIS1 target genes. Predicted pharmacokinetics properties of the compounds matched drug-likeness criteria, without cytotoxicity or mutagenicity observed.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Simon Grootendorst, Jonathan de Wilde, Birgit van Dooijeweert, Annelies van Vuren, Wouter van Solinge, Roger Schutgens, Richard van Wijk, Marije Bartels
Summary: Rare hereditary anemias (RHA) are characterized by impaired red blood cell production or decreased survival, often leading to disturbances in iron metabolism and erythropoiesis, resulting in iron overload or exacerbation of chronic anemia. Iron overload is a significant issue in both transfusion-dependent and non-transfusion-dependent patients with RHA, suggesting common defects in erythroid development linked to intrinsic iron metabolism defects. Key regulators involved in the interaction between iron and erythropoiesis are discussed in the context of RHA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Patrick Coulombe, Grace Cole, Amanda Fentiman, Jeremy D. K. Parker, Eric Yung, Misha Bilenky, Lemlem Degefie, Patrick Lac, Maggie Y. M. Ling, Derek Tam, R. Keith Humphries, Aly Karsan
Summary: The authors identify Meis1 as an early regulator in the specification of hemogenic cells from arterial endothelium. Meis1 is crucial for the formation of functional Runx1-expressing hemogenic endothelial cells, which are necessary for the emergence of hematopoietic stem and progenitor cells.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Anna Zaninoni, Roberta Marra, Elisa Fermo, Dario Consonni, Immacolata Andolfo, Anna Paola Marcello, Barbara Eleni Rosato, Cristina Vercellati, Wilma Barcellini, Achille Iolascon, Paola Bianchi, Roberta Russo
Summary: This study investigated iron homeostasis and dyserythropoiesis in pyruvate kinase deficiency (PKD) patients. The results showed that PKD patients had intermediate levels of sTfR, hepcidin, ERFE, and EPO between CDAII and HS. PKD patients also exhibited higher levels of sTfR and similar levels of EPO compared to CDAII, but both higher than in HS.
SCIENTIFIC REPORTS
(2023)
Review
Cell Biology
Mingzhu Jiang, Shuang Xu, Mi Bai, Aihua Zhang
Summary: Cell proliferation and differentiation are crucial for reproduction and growth, with MEIS1 identified as a significant transcription factor involved in regulating these processes. MEIS1 can exhibit both pro-proliferative and anti-proliferative effects in different cell types and diseases, suggesting its potential as a therapeutic target for various conditions.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Pascal Vong, Hakim Ouled-Haddou, Loic Garcon
Summary: Numerous studies have emphasized the importance of post-translational modifications in regulating cellular processes such as cell proliferation, differentiation, and death. Acetylation, a type of modification, alters the properties and activity of proteins and plays a role in their stability, localization, and interaction with other proteins. Histone deacetylases (HDACs) are responsible for the deacetylation of many proteins, both functional and structural, in the nucleus and cytoplasm. They are involved in crucial cellular processes, including hematopoiesis, where they finely regulate the differentiation of stem cells and progenitors. Understanding the role of HDACs in hematopoiesis is important, particularly considering their potential as targets for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Tao Wang, Weiqi Zhang, Wanshu Chen, Jie Meng, Qinglin Hu, Jian Liu, Tao Wen, Bing Han, Haiyan Xu
Summary: In this study, a novel function of As4S4 nanoparticles in promoting terminal erythropoiesis in MDS patients was revealed. These nanoparticles triggered the phosphorylation of eIF2α, leading to an increase in global protein synthesis and mitigated oxidative stress in bone marrow cells. Overall, ee-As4S4 hold significant potentials for the effective differentiation of erythroblasts in refractory anemia.
Article
Multidisciplinary Sciences
Yohko Kitagawa, Akihiro Ikenaka, Ryohichi Sugimura, Akira Niwa, Megumu K. Saito
Summary: Cell differentiation is achieved through the acquisition of a cell type-specific transcriptional program and epigenetic landscape. Research has shown that the patterning of cell type-specific enhancers occurs before cell fate decisions, but the mechanisms for initiating cell specification and properly restricting differentiation remain unclear. In this study, the activation of hematopoietic enhancers during arterialization of hemogenic endothelium, a key step in hematopoiesis, was observed. It was also found that the transcription factor ZEB2, involved in arterial endothelial cell regulation, and the hematopoietic regulator MEIS1 are independently required for activating these enhancers. Deficiency in ZEB2 or MEIS1 impairs hematopoietic cell development. These findings suggest that multiple regulators expressed during early development contribute non-redundantly to the establishment of the hematopoietic enhancer landscape, thereby restricting cell differentiation despite the unrestricted expression of these regulators in hematopoietic cells.
Article
Biochemistry & Molecular Biology
M. Kyle Cromer, Joab Camarena, Renata M. Martin, Benjamin J. Lesch, Christopher A. Vakulskas, Nicole M. Bode, Gavin Kurgan, Michael A. Collingwood, Garrett R. Rettig, Mark A. Behlke, Viktor T. Lemgart, Yankai Zhang, Ankush Goyal, Feifei Zhao, Ezequiel Ponce, Waracharee Srifa, Rasmus O. Bak, Naoya Uchida, Ravindra Majeti, Vivien A. Sheehan, John F. Tisdale, Daniel P. Dever, Matthew H. Porteus
Summary: The study presents a novel genome editing strategy that can restore the balance of beta-globin:alpha-globin in human hematopoietic stem cells from patients with beta-thalassemia, leading to the functional restoration of adult hemoglobin tetramers in patient-derived red blood cells.
Article
Biochemistry & Molecular Biology
Valentina Pastori, Serena Pozzi, Agata Labedz, Sajeela Ahmed, Antonella Ellena Ronchi
Summary: Nuclear receptors (NRs) are a family of ligand-regulated transcription factors involved in various physiological processes. They act as transcriptional activators or repressors upon DNA binding and can be modulated by small molecules. Understanding the role of NRs in erythropoiesis is crucial for disease treatment and blood cell culture research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Zheng Cao, Chengcheng Huang, Fumin Lu, Xuequan Jiang, Yong Hu, Cheng Cao, Zijing Liu
Summary: This study reveals the important role of the transcription factor Meis1 in regulating the development of nociceptors, which play a key role in transmitting pain and itch signals. Meis1 is required for the development of nociceptors and regulates the expression of genes related to sensory perception of pain and nervous system development.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Yining Liu, Jiamin Li, Ning Xu, Hang Yu, Liling Gong, Qingsui Li, Zhenyu Yang, Sijia Li, Jiming Yang, Di Huang, Yadong Xue, Genlong Xue, Jiali Liu, Haixin Chen, Ruijie Zhang, Anqi Li, Yiming Zhao, PengYu Li, Ming Li, Mingbin Liu, Ning Wang, Benzhi Cai
Summary: This study found that overexpression of Meis1 can improve the electrophysiological function of the heart after myocardial infarction and restore the expression level of Na(V)1.5. In addition, the study also found that E3 ubiquitin ligase CDC20 can mediate the ubiquitination and degradation of Meis1, leading to the remodeling of cardiac electrical conduction.
JOURNAL OF ADVANCED RESEARCH
(2022)
Review
Medicine, Research & Experimental
Maozhong Yao, Yong Gu, Zhaoxin Yang, Keyan Zhong, Zhanjuan Chen
Summary: Meis1 was first discovered in 1995 and found to be highly expressed in human cells, located on chromosome 2p14 encoding a protein with six domains. It interacts with other proteins like homeobox and plays a critical role in cell proliferation in leukemia and solid tumors.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Review
Cardiac & Cardiovascular Systems
Milton Packer
Summary: SGLT2 inhibitors reduce the risk of heart failure events by enhancing erythropoiesis. This enhancement is hypothesized to be due to increased oxygenation in the kidneys, increased oxygen consumption and localized hypoxia, increased iron mobilization, and direct activation of erythropoietin gene transcription. The liver may also contribute to erythropoietin production during SGLT2 inhibition. The mechanism linking erythropoiesis to cardioprotection may involve the upregulation of SIRT1 and its effects on HIF-2 alpha expression and erythropoietin synthesis.
EUROPEAN HEART JOURNAL
(2023)
Editorial Material
Developmental Biology
Anna M. Ranzoni, Ana Cvejic
Review
Cell Biology
Paulina M. Strzelecka, Anna M. Ranzoni, Ana Cvejic
DISEASE MODELS & MECHANISMS
(2018)
Article
Cardiac & Cardiovascular Systems
Marwa Mahmoud, Celine Souilhol, Jovana Serbanovic-Canic, Paul Evans
CARDIOVASCULAR DRUGS AND THERAPY
(2019)
Editorial Material
Cardiac & Cardiovascular Systems
Jovana Serbanovic-Canic, Celine Souilhol, Paul C. Evans
CARDIOVASCULAR RESEARCH
(2019)
Article
Cell Biology
Ioannis Xanthis, Celine Souilhol, Jovana Serbanovic-Canic, Hannah Roddie, Antreas C. Kalli, Maria Fragiadaki, Raymond Wong, Dhruv R. Shah, Janet A. Askari, Lindsay Canham, Nasreen Akhtar, Shuang Feng, Victoria Ridger, Jonathan Waltho, Emmanuel Pinteaux, Martin J. Humphries, Matthew T. Bryan, Paul C. Evans
JOURNAL OF CELL SCIENCE
(2019)
Review
Biochemistry & Molecular Biology
Anna M. Ranzoni, Paulina M. Strzelecka, Ana Cvejic
SINGLE CELL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Felipe A. Vieira Braga, Gozde Kar, Marijn Berg, Orestes A. Carpaij, Krzysztof Polanski, Lukas M. Simon, Sharon Brouwer, Tomas Gomes, Laura Hesse, Jian Jiang, Eirini S. Fasouli, Mirjana Efremova, Roser Vento-Tormo, Carlos Talavera-Lopez, Marnix R. Jonker, Karen Affleck, Subarna Palit, Paulina M. Strzelecka, Helen V. Firth, Krishnaa T. Mahbubani, Ana Cvejic, Kerstin B. Meyer, Kourosh Saeb-Parsy, Marjan Luinge, Corry-Anke Brandsma, Wim Timens, Ilias Angelidis, Maximilian Strunz, Gerard H. Koppelman, Antoon J. van Oosterhout, Herbert B. Schiller, Fabian J. Theis, Maarten van den Berge, Martijn C. Nawijn, Sarah A. Teichmann
Review
Cardiac & Cardiovascular Systems
Celine Souilhol, Jovana Serbanovic-Canic, Maria Fragiadaki, Timothy J. Chico, Victoria Ridger, Hannah Roddie, Paul C. Evans
NATURE REVIEWS CARDIOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Celine Souilhol, Ismael Gauci, Shuang Feng, Blanca Tardajos Ayllon, Marwa Mahmoud, Lindsay Canham, Maria Fragiadaki, Jovana Serbanovic-Canic, Victoria Ridger, Paul Charles Evans
CARDIOVASCULAR RESEARCH
(2020)
Correction
Multidisciplinary Sciences
Marwa M. Mahmoud, Jovana Serbanovic-Canic, Shuang Feng, Celine Souilhol, Rouyu Xing, Sarah Hsiao, Akiko Mammoto, Jing Chen, Markus Ariaans, Sheila E. Francis, Kim Van der Heiden, Victoria Ridger, Paul C. Evans
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Ioana Bica, Helena Andres-Terre, Ana Cvejic, Pietro Lio
SCIENTIFIC REPORTS
(2020)
Article
Cell & Tissue Engineering
Anna Maria Ranzoni, Andrea Tangherloni, Ivan Berest, Simone Giovanni Riva, Brynelle Myers, Paulina M. Strzelecka, Jiarui Xu, Elisa Panada, Irina Mohorianu, Judith B. Zaugg, Ana Cvejic
Summary: Using single-cell RNA sequencing and single-cell assay for transposase-accessible chromatin sequencing, researchers identified three highly proliferative oligopotent progenitor populations downstream of hematopoietic stem cells (HSCs)/multipotent progenitors (MPPs) in human fetal liver and bone marrow. They observed dynamic changes in chromatin accessibility and lineage-specific transcription factor activity along the differentiation trajectory, providing a useful framework for future investigation of human developmental hematopoiesis.
Review
Pharmacology & Pharmacy
George Bowley, Elizabeth Kugler, Rob Wilkinson, Allan Lawrie, Freek van Eeden, Tim J. A. Chico, Paul C. Evans, Emily S. Noel, Jovana Serbanovic-Canic
Summary: The use of zebrafish models in studying cardiovascular development and disease mechanisms has become increasingly popular. By comparing the anatomy and physiology of zebrafish and mammalian cardiovascular systems, researchers have found that zebrafish have unique advantages in simulating cardiac and vascular pathologies.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Giovanni Canu, Emmanouil Athanasiadis, Rodrigo A. Grandy, Jose Garcia-Bernardo, Paulina M. Strzelecka, Ludovic Vallier, Daniel Ortmann, Ana Cvejic
Article
Immunology
Pedro P. Hernandez, Paulina M. Strzelecka, Emmanouil Athanasiadis, Dominic Hall, Ana F. Robalo, Catherine M. Collins, Pierre Boudinot, Jean-Pierre Levraud, Ana Cvejic
SCIENCE IMMUNOLOGY
(2018)